MiR-99b-5p suppressed proliferation of human osteoblasts by targeting FGFR3 in osteoporosis
Osteoporosis is a common skeletal disease characterized by reduced bone mass partially caused by an imbalance between bone resorption and formation. Considering the potential role of microRNAs (miRNAs) in osteoporosis, we attempted to identify deregulated miRNA that participates in the pathogenesis...
Gespeichert in:
| Veröffentlicht in: | Human cell : official journal of Human Cell Research Society 2021-09, Vol.34 (5), p.1398-1409 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1409 |
|---|---|
| container_issue | 5 |
| container_start_page | 1398 |
| container_title | Human cell : official journal of Human Cell Research Society |
| container_volume | 34 |
| creator | Ding, Muliang Liu, Bo Chen, Xia Ouyang, Zhengxiao Peng, Dan Zhou, Yu |
| description | Osteoporosis is a common skeletal disease characterized by reduced bone mass partially caused by an imbalance between bone resorption and formation. Considering the potential role of microRNAs (miRNAs) in osteoporosis, we attempted to identify deregulated miRNA that participates in the pathogenesis of osteoporosis. We analyzed online datasets for differentially expressed miRNAs and predicted deregulated miRNA target genes, applied these genes for signaling pathway enrichment annotation, and selected the possible miR-99b-5p/FGFR3 axis. Within osteoporosis bone tissues, miR-99b-5p was upregulated and FGFR3 was downregulated. miR-99b-5p overexpression inhibited osteoblast proliferation and osteogenesis-related genes expression, whereas FGFR3 overexpression exerted opposite effects upon the proliferation of osteoblasts and osteogenesis-related genes expression. By direct targeting, miR-99b-5p inhibited FGFR3 expression. Moreover, FGFR3 silencing significantly reversed the roles of miR-99b-5p inhibition in the proliferation of osteoblasts and osteogenesis-related genes expression. In conclusion, we identify a deregulated miRNA/mRNA axis in osteoporosis and osteogenic differentiation, namely the miR-99b-5p/FGFR3 axis; through targeting FGFR3, miR-99b-5p inhibits osteoblast proliferation and activity, which might subsequently affect the bone formation in osteoporosis progression. |
| doi_str_mv | 10.1007/s13577-021-00567-3 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2544160656</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2544160656</sourcerecordid><originalsourceid>FETCH-LOGICAL-c399t-1b25343cb1ab61fe6f4b3ea7254628bf9a3a4e8fc51b525866c28a70a33671d53</originalsourceid><addsrcrecordid>eNp9kLtOwzAUhi0EoqXwAgwoEguLwY5vyYgqWpCKkCqYGCw7dUqq3PBJhr49LikXMTD5SP7O798fQueUXFNC1A1QJpTCJKaYECEVZgdoTBVPMVGKH_6aR-gEYEMIF1zGx2jEOBVScjJGr4_FEqepxaKNoG9b7wDcKmp9Uxa586Yrmjpq8uitr0wYoHONLQ10ENlt1Bm_dl1Rr6PZfLZkUbEn2sY3UMApOspNCe5sf07Qy-zueXqPF0_zh-ntAmcsTTtMbSwYZ5mlxkqaO5lzy5xR8a5sYvPUMMNdkmeCWhGLRMosTowihjGp6EqwCboackPr995Bp6sCMleWpnZNDzoEcSqJFDKgl3_QTdP7OrQLlEgoUSQhgYoHKgv_AO9y3fqiMn6rKdE79XpQr4N6_ales7B0sY_ubeVW3ytfrgPABgDCVb12_uftf2I_AFTMjZk</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2558107080</pqid></control><display><type>article</type><title>MiR-99b-5p suppressed proliferation of human osteoblasts by targeting FGFR3 in osteoporosis</title><source>MEDLINE</source><source>Springer Nature - Complete Springer Journals</source><creator>Ding, Muliang ; Liu, Bo ; Chen, Xia ; Ouyang, Zhengxiao ; Peng, Dan ; Zhou, Yu</creator><creatorcontrib>Ding, Muliang ; Liu, Bo ; Chen, Xia ; Ouyang, Zhengxiao ; Peng, Dan ; Zhou, Yu</creatorcontrib><description>Osteoporosis is a common skeletal disease characterized by reduced bone mass partially caused by an imbalance between bone resorption and formation. Considering the potential role of microRNAs (miRNAs) in osteoporosis, we attempted to identify deregulated miRNA that participates in the pathogenesis of osteoporosis. We analyzed online datasets for differentially expressed miRNAs and predicted deregulated miRNA target genes, applied these genes for signaling pathway enrichment annotation, and selected the possible miR-99b-5p/FGFR3 axis. Within osteoporosis bone tissues, miR-99b-5p was upregulated and FGFR3 was downregulated. miR-99b-5p overexpression inhibited osteoblast proliferation and osteogenesis-related genes expression, whereas FGFR3 overexpression exerted opposite effects upon the proliferation of osteoblasts and osteogenesis-related genes expression. By direct targeting, miR-99b-5p inhibited FGFR3 expression. Moreover, FGFR3 silencing significantly reversed the roles of miR-99b-5p inhibition in the proliferation of osteoblasts and osteogenesis-related genes expression. In conclusion, we identify a deregulated miRNA/mRNA axis in osteoporosis and osteogenic differentiation, namely the miR-99b-5p/FGFR3 axis; through targeting FGFR3, miR-99b-5p inhibits osteoblast proliferation and activity, which might subsequently affect the bone formation in osteoporosis progression.</description><identifier>ISSN: 1749-0774</identifier><identifier>ISSN: 0914-7470</identifier><identifier>EISSN: 1749-0774</identifier><identifier>DOI: 10.1007/s13577-021-00567-3</identifier><identifier>PMID: 34156640</identifier><language>eng</language><publisher>Singapore: Springer Singapore</publisher><subject>Aged ; Biomedical and Life Sciences ; Bone growth ; Bone mass ; Bone resorption ; Cell Biology ; Cell Proliferation - genetics ; Cells, Cultured ; Female ; Fibroblast growth factor receptors ; Gene Expression - genetics ; Gene Expression Regulation, Developmental - genetics ; Gene Expression Regulation, Developmental - physiology ; Gynecology ; Humans ; Life Sciences ; Male ; MicroRNAs ; MicroRNAs - physiology ; Middle Aged ; miRNA ; mRNA ; Oncology ; Osteoblastogenesis ; Osteoblasts ; Osteoblasts - physiology ; Osteogenesis ; Osteogenesis - genetics ; Osteogenesis - physiology ; Osteoporosis ; Osteoporosis - genetics ; Osteoporosis - pathology ; Receptor, Fibroblast Growth Factor, Type 3 - genetics ; Receptor, Fibroblast Growth Factor, Type 3 - metabolism ; Reproductive Medicine ; Research Article ; Signal transduction ; Signal Transduction - genetics ; Signal Transduction - physiology ; Stem Cells ; Surgery</subject><ispartof>Human cell : official journal of Human Cell Research Society, 2021-09, Vol.34 (5), p.1398-1409</ispartof><rights>Japan Human Cell Society 2021</rights><rights>2021. Japan Human Cell Society.</rights><rights>Japan Human Cell Society 2021.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c399t-1b25343cb1ab61fe6f4b3ea7254628bf9a3a4e8fc51b525866c28a70a33671d53</citedby><cites>FETCH-LOGICAL-c399t-1b25343cb1ab61fe6f4b3ea7254628bf9a3a4e8fc51b525866c28a70a33671d53</cites><orcidid>0000-0003-2339-7568</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s13577-021-00567-3$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s13577-021-00567-3$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27903,27904,41467,42536,51298</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34156640$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ding, Muliang</creatorcontrib><creatorcontrib>Liu, Bo</creatorcontrib><creatorcontrib>Chen, Xia</creatorcontrib><creatorcontrib>Ouyang, Zhengxiao</creatorcontrib><creatorcontrib>Peng, Dan</creatorcontrib><creatorcontrib>Zhou, Yu</creatorcontrib><title>MiR-99b-5p suppressed proliferation of human osteoblasts by targeting FGFR3 in osteoporosis</title><title>Human cell : official journal of Human Cell Research Society</title><addtitle>Human Cell</addtitle><addtitle>Hum Cell</addtitle><description>Osteoporosis is a common skeletal disease characterized by reduced bone mass partially caused by an imbalance between bone resorption and formation. Considering the potential role of microRNAs (miRNAs) in osteoporosis, we attempted to identify deregulated miRNA that participates in the pathogenesis of osteoporosis. We analyzed online datasets for differentially expressed miRNAs and predicted deregulated miRNA target genes, applied these genes for signaling pathway enrichment annotation, and selected the possible miR-99b-5p/FGFR3 axis. Within osteoporosis bone tissues, miR-99b-5p was upregulated and FGFR3 was downregulated. miR-99b-5p overexpression inhibited osteoblast proliferation and osteogenesis-related genes expression, whereas FGFR3 overexpression exerted opposite effects upon the proliferation of osteoblasts and osteogenesis-related genes expression. By direct targeting, miR-99b-5p inhibited FGFR3 expression. Moreover, FGFR3 silencing significantly reversed the roles of miR-99b-5p inhibition in the proliferation of osteoblasts and osteogenesis-related genes expression. In conclusion, we identify a deregulated miRNA/mRNA axis in osteoporosis and osteogenic differentiation, namely the miR-99b-5p/FGFR3 axis; through targeting FGFR3, miR-99b-5p inhibits osteoblast proliferation and activity, which might subsequently affect the bone formation in osteoporosis progression.</description><subject>Aged</subject><subject>Biomedical and Life Sciences</subject><subject>Bone growth</subject><subject>Bone mass</subject><subject>Bone resorption</subject><subject>Cell Biology</subject><subject>Cell Proliferation - genetics</subject><subject>Cells, Cultured</subject><subject>Female</subject><subject>Fibroblast growth factor receptors</subject><subject>Gene Expression - genetics</subject><subject>Gene Expression Regulation, Developmental - genetics</subject><subject>Gene Expression Regulation, Developmental - physiology</subject><subject>Gynecology</subject><subject>Humans</subject><subject>Life Sciences</subject><subject>Male</subject><subject>MicroRNAs</subject><subject>MicroRNAs - physiology</subject><subject>Middle Aged</subject><subject>miRNA</subject><subject>mRNA</subject><subject>Oncology</subject><subject>Osteoblastogenesis</subject><subject>Osteoblasts</subject><subject>Osteoblasts - physiology</subject><subject>Osteogenesis</subject><subject>Osteogenesis - genetics</subject><subject>Osteogenesis - physiology</subject><subject>Osteoporosis</subject><subject>Osteoporosis - genetics</subject><subject>Osteoporosis - pathology</subject><subject>Receptor, Fibroblast Growth Factor, Type 3 - genetics</subject><subject>Receptor, Fibroblast Growth Factor, Type 3 - metabolism</subject><subject>Reproductive Medicine</subject><subject>Research Article</subject><subject>Signal transduction</subject><subject>Signal Transduction - genetics</subject><subject>Signal Transduction - physiology</subject><subject>Stem Cells</subject><subject>Surgery</subject><issn>1749-0774</issn><issn>0914-7470</issn><issn>1749-0774</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kLtOwzAUhi0EoqXwAgwoEguLwY5vyYgqWpCKkCqYGCw7dUqq3PBJhr49LikXMTD5SP7O798fQueUXFNC1A1QJpTCJKaYECEVZgdoTBVPMVGKH_6aR-gEYEMIF1zGx2jEOBVScjJGr4_FEqepxaKNoG9b7wDcKmp9Uxa586Yrmjpq8uitr0wYoHONLQ10ENlt1Bm_dl1Rr6PZfLZkUbEn2sY3UMApOspNCe5sf07Qy-zueXqPF0_zh-ntAmcsTTtMbSwYZ5mlxkqaO5lzy5xR8a5sYvPUMMNdkmeCWhGLRMosTowihjGp6EqwCboackPr995Bp6sCMleWpnZNDzoEcSqJFDKgl3_QTdP7OrQLlEgoUSQhgYoHKgv_AO9y3fqiMn6rKdE79XpQr4N6_ales7B0sY_ubeVW3ytfrgPABgDCVb12_uftf2I_AFTMjZk</recordid><startdate>20210901</startdate><enddate>20210901</enddate><creator>Ding, Muliang</creator><creator>Liu, Bo</creator><creator>Chen, Xia</creator><creator>Ouyang, Zhengxiao</creator><creator>Peng, Dan</creator><creator>Zhou, Yu</creator><general>Springer Singapore</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-2339-7568</orcidid></search><sort><creationdate>20210901</creationdate><title>MiR-99b-5p suppressed proliferation of human osteoblasts by targeting FGFR3 in osteoporosis</title><author>Ding, Muliang ; Liu, Bo ; Chen, Xia ; Ouyang, Zhengxiao ; Peng, Dan ; Zhou, Yu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c399t-1b25343cb1ab61fe6f4b3ea7254628bf9a3a4e8fc51b525866c28a70a33671d53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Aged</topic><topic>Biomedical and Life Sciences</topic><topic>Bone growth</topic><topic>Bone mass</topic><topic>Bone resorption</topic><topic>Cell Biology</topic><topic>Cell Proliferation - genetics</topic><topic>Cells, Cultured</topic><topic>Female</topic><topic>Fibroblast growth factor receptors</topic><topic>Gene Expression - genetics</topic><topic>Gene Expression Regulation, Developmental - genetics</topic><topic>Gene Expression Regulation, Developmental - physiology</topic><topic>Gynecology</topic><topic>Humans</topic><topic>Life Sciences</topic><topic>Male</topic><topic>MicroRNAs</topic><topic>MicroRNAs - physiology</topic><topic>Middle Aged</topic><topic>miRNA</topic><topic>mRNA</topic><topic>Oncology</topic><topic>Osteoblastogenesis</topic><topic>Osteoblasts</topic><topic>Osteoblasts - physiology</topic><topic>Osteogenesis</topic><topic>Osteogenesis - genetics</topic><topic>Osteogenesis - physiology</topic><topic>Osteoporosis</topic><topic>Osteoporosis - genetics</topic><topic>Osteoporosis - pathology</topic><topic>Receptor, Fibroblast Growth Factor, Type 3 - genetics</topic><topic>Receptor, Fibroblast Growth Factor, Type 3 - metabolism</topic><topic>Reproductive Medicine</topic><topic>Research Article</topic><topic>Signal transduction</topic><topic>Signal Transduction - genetics</topic><topic>Signal Transduction - physiology</topic><topic>Stem Cells</topic><topic>Surgery</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ding, Muliang</creatorcontrib><creatorcontrib>Liu, Bo</creatorcontrib><creatorcontrib>Chen, Xia</creatorcontrib><creatorcontrib>Ouyang, Zhengxiao</creatorcontrib><creatorcontrib>Peng, Dan</creatorcontrib><creatorcontrib>Zhou, Yu</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Human cell : official journal of Human Cell Research Society</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ding, Muliang</au><au>Liu, Bo</au><au>Chen, Xia</au><au>Ouyang, Zhengxiao</au><au>Peng, Dan</au><au>Zhou, Yu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>MiR-99b-5p suppressed proliferation of human osteoblasts by targeting FGFR3 in osteoporosis</atitle><jtitle>Human cell : official journal of Human Cell Research Society</jtitle><stitle>Human Cell</stitle><addtitle>Hum Cell</addtitle><date>2021-09-01</date><risdate>2021</risdate><volume>34</volume><issue>5</issue><spage>1398</spage><epage>1409</epage><pages>1398-1409</pages><issn>1749-0774</issn><issn>0914-7470</issn><eissn>1749-0774</eissn><abstract>Osteoporosis is a common skeletal disease characterized by reduced bone mass partially caused by an imbalance between bone resorption and formation. Considering the potential role of microRNAs (miRNAs) in osteoporosis, we attempted to identify deregulated miRNA that participates in the pathogenesis of osteoporosis. We analyzed online datasets for differentially expressed miRNAs and predicted deregulated miRNA target genes, applied these genes for signaling pathway enrichment annotation, and selected the possible miR-99b-5p/FGFR3 axis. Within osteoporosis bone tissues, miR-99b-5p was upregulated and FGFR3 was downregulated. miR-99b-5p overexpression inhibited osteoblast proliferation and osteogenesis-related genes expression, whereas FGFR3 overexpression exerted opposite effects upon the proliferation of osteoblasts and osteogenesis-related genes expression. By direct targeting, miR-99b-5p inhibited FGFR3 expression. Moreover, FGFR3 silencing significantly reversed the roles of miR-99b-5p inhibition in the proliferation of osteoblasts and osteogenesis-related genes expression. In conclusion, we identify a deregulated miRNA/mRNA axis in osteoporosis and osteogenic differentiation, namely the miR-99b-5p/FGFR3 axis; through targeting FGFR3, miR-99b-5p inhibits osteoblast proliferation and activity, which might subsequently affect the bone formation in osteoporosis progression.</abstract><cop>Singapore</cop><pub>Springer Singapore</pub><pmid>34156640</pmid><doi>10.1007/s13577-021-00567-3</doi><tpages>12</tpages><orcidid>https://orcid.org/0000-0003-2339-7568</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1749-0774 |
| ispartof | Human cell : official journal of Human Cell Research Society, 2021-09, Vol.34 (5), p.1398-1409 |
| issn | 1749-0774 0914-7470 1749-0774 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2544160656 |
| source | MEDLINE; Springer Nature - Complete Springer Journals |
| subjects | Aged Biomedical and Life Sciences Bone growth Bone mass Bone resorption Cell Biology Cell Proliferation - genetics Cells, Cultured Female Fibroblast growth factor receptors Gene Expression - genetics Gene Expression Regulation, Developmental - genetics Gene Expression Regulation, Developmental - physiology Gynecology Humans Life Sciences Male MicroRNAs MicroRNAs - physiology Middle Aged miRNA mRNA Oncology Osteoblastogenesis Osteoblasts Osteoblasts - physiology Osteogenesis Osteogenesis - genetics Osteogenesis - physiology Osteoporosis Osteoporosis - genetics Osteoporosis - pathology Receptor, Fibroblast Growth Factor, Type 3 - genetics Receptor, Fibroblast Growth Factor, Type 3 - metabolism Reproductive Medicine Research Article Signal transduction Signal Transduction - genetics Signal Transduction - physiology Stem Cells Surgery |
| title | MiR-99b-5p suppressed proliferation of human osteoblasts by targeting FGFR3 in osteoporosis |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-21T18%3A16%3A25IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=MiR-99b-5p%20suppressed%20proliferation%20of%20human%20osteoblasts%20by%20targeting%20FGFR3%20in%20osteoporosis&rft.jtitle=Human%20cell%20:%20official%20journal%20of%20Human%20Cell%20Research%20Society&rft.au=Ding,%20Muliang&rft.date=2021-09-01&rft.volume=34&rft.issue=5&rft.spage=1398&rft.epage=1409&rft.pages=1398-1409&rft.issn=1749-0774&rft.eissn=1749-0774&rft_id=info:doi/10.1007/s13577-021-00567-3&rft_dat=%3Cproquest_cross%3E2544160656%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2558107080&rft_id=info:pmid/34156640&rfr_iscdi=true |