Specific recognition of protein by deep eutectic solvent–based magnetic β-cyclodextrin molecularly imprinted polymer
A magnetic β-cyclodextrin (MCD) surface molecularly imprinted polymer (MIP) based on deep eutectic solvents (DESs) as cross-linker and functional monomer (MCD@DES-MIP) was successfully synthesized for the specific recognition of bovine hemoglobin (BHb). The adsorption behavior of MCD@DES-MIP for BHb...
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| Veröffentlicht in: | Mikrochimica acta (1966) 2021-07, Vol.188 (7), p.232-232, Article 232 |
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| container_title | Mikrochimica acta (1966) |
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| creator | He, Xiyan Wang, Yuzhi Li, Heqiong Chen, Jing Liu, Ziwei Xu, Fangting Zhou, Yigang |
| description | A magnetic β-cyclodextrin (MCD) surface molecularly imprinted polymer (MIP) based on deep eutectic solvents (DESs) as cross-linker and functional monomer (MCD@DES-MIP) was successfully synthesized for the specific recognition of bovine hemoglobin (BHb). The adsorption behavior of MCD@DES-MIP for BHb was investigated by adsorption thermodynamics, adsorption kinetics, and pH control experiments. The maximum adsorption capacity of MCD@DES-MIP for BHb under the optimized conditions was 195.94 mg g
−1
and the imprinting factor was 4.68. In addition, the competitive adsorption experiments demonstrated that MCD@DES-MIP showed excellent selective extraction ability for BHb in the binary mixture of BHb and bovine serum albumin (BSA). The actual sample analysis manifested that MCD@DES-MIP effectively separated BHb from complex samples. The results of circular dichroism spectra proved that the secondary structure of BHb did not change during elution. The result indicated that MCD@DES-MIP can be used as a new imprinting material for the separation and purification of BHb.
Graphical abstract
Magnetic imprinted microspheres (MCD@DES-MIP) were prepared by free radical polymerization using magnetic β-cyclodextrin (MCD) as carrier, deep eutectic solvents (DESs) as functional monomer and cross-linker. MCD@DES-MIP show high adsorption capacity and excellent selectivity for BHb. |
| doi_str_mv | 10.1007/s00604-021-04887-x |
| format | Article |
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−1
and the imprinting factor was 4.68. In addition, the competitive adsorption experiments demonstrated that MCD@DES-MIP showed excellent selective extraction ability for BHb in the binary mixture of BHb and bovine serum albumin (BSA). The actual sample analysis manifested that MCD@DES-MIP effectively separated BHb from complex samples. The results of circular dichroism spectra proved that the secondary structure of BHb did not change during elution. The result indicated that MCD@DES-MIP can be used as a new imprinting material for the separation and purification of BHb.
Graphical abstract
Magnetic imprinted microspheres (MCD@DES-MIP) were prepared by free radical polymerization using magnetic β-cyclodextrin (MCD) as carrier, deep eutectic solvents (DESs) as functional monomer and cross-linker. MCD@DES-MIP show high adsorption capacity and excellent selectivity for BHb.</description><identifier>ISSN: 0026-3672</identifier><identifier>EISSN: 1436-5073</identifier><identifier>DOI: 10.1007/s00604-021-04887-x</identifier><identifier>PMID: 34137917</identifier><language>eng</language><publisher>Vienna: Springer Vienna</publisher><subject>Adsorption ; Analytical Chemistry ; Animals ; beta-Cyclodextrins - metabolism ; Binary mixtures ; Cattle ; Characterization and Evaluation of Materials ; Chemistry ; Chemistry and Materials Science ; Cyclodextrins ; Deep Eutectic Solvents - chemistry ; Dichroism ; Hemoglobin ; Humans ; Imprinted polymers ; Microengineering ; Molecularly Imprinted Polymers - metabolism ; Nanochemistry ; Nanotechnology ; Original Paper ; pH control ; Recognition ; Serum albumin ; Solvents ; Solvents - chemistry</subject><ispartof>Mikrochimica acta (1966), 2021-07, Vol.188 (7), p.232-232, Article 232</ispartof><rights>The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature 2021</rights><rights>The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature 2021.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c375t-77f996eb0d702126254edcf0cd5168383c33ea1ae4b88f7cd57f8bdf3eb75f5b3</citedby><cites>FETCH-LOGICAL-c375t-77f996eb0d702126254edcf0cd5168383c33ea1ae4b88f7cd57f8bdf3eb75f5b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00604-021-04887-x$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00604-021-04887-x$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27923,27924,41487,42556,51318</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34137917$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>He, Xiyan</creatorcontrib><creatorcontrib>Wang, Yuzhi</creatorcontrib><creatorcontrib>Li, Heqiong</creatorcontrib><creatorcontrib>Chen, Jing</creatorcontrib><creatorcontrib>Liu, Ziwei</creatorcontrib><creatorcontrib>Xu, Fangting</creatorcontrib><creatorcontrib>Zhou, Yigang</creatorcontrib><title>Specific recognition of protein by deep eutectic solvent–based magnetic β-cyclodextrin molecularly imprinted polymer</title><title>Mikrochimica acta (1966)</title><addtitle>Microchim Acta</addtitle><addtitle>Mikrochim Acta</addtitle><description>A magnetic β-cyclodextrin (MCD) surface molecularly imprinted polymer (MIP) based on deep eutectic solvents (DESs) as cross-linker and functional monomer (MCD@DES-MIP) was successfully synthesized for the specific recognition of bovine hemoglobin (BHb). The adsorption behavior of MCD@DES-MIP for BHb was investigated by adsorption thermodynamics, adsorption kinetics, and pH control experiments. The maximum adsorption capacity of MCD@DES-MIP for BHb under the optimized conditions was 195.94 mg g
−1
and the imprinting factor was 4.68. In addition, the competitive adsorption experiments demonstrated that MCD@DES-MIP showed excellent selective extraction ability for BHb in the binary mixture of BHb and bovine serum albumin (BSA). The actual sample analysis manifested that MCD@DES-MIP effectively separated BHb from complex samples. The results of circular dichroism spectra proved that the secondary structure of BHb did not change during elution. The result indicated that MCD@DES-MIP can be used as a new imprinting material for the separation and purification of BHb.
Graphical abstract
Magnetic imprinted microspheres (MCD@DES-MIP) were prepared by free radical polymerization using magnetic β-cyclodextrin (MCD) as carrier, deep eutectic solvents (DESs) as functional monomer and cross-linker. MCD@DES-MIP show high adsorption capacity and excellent selectivity for BHb.</description><subject>Adsorption</subject><subject>Analytical Chemistry</subject><subject>Animals</subject><subject>beta-Cyclodextrins - metabolism</subject><subject>Binary mixtures</subject><subject>Cattle</subject><subject>Characterization and Evaluation of Materials</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Cyclodextrins</subject><subject>Deep Eutectic Solvents - chemistry</subject><subject>Dichroism</subject><subject>Hemoglobin</subject><subject>Humans</subject><subject>Imprinted polymers</subject><subject>Microengineering</subject><subject>Molecularly Imprinted Polymers - metabolism</subject><subject>Nanochemistry</subject><subject>Nanotechnology</subject><subject>Original Paper</subject><subject>pH control</subject><subject>Recognition</subject><subject>Serum albumin</subject><subject>Solvents</subject><subject>Solvents - chemistry</subject><issn>0026-3672</issn><issn>1436-5073</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU2O1DAQhS0EYnoGLsACRWLDxlC2E9u9RCP-pJFYAGsrccqtjJw42A50dnMHbsJBOAQnwU0GkFiwslT-3nM9P0IeMXjGANTzBCChpsAZhVprRY93yI7VQtIGlLhLdgBcUiEVPyPnKV0DMCV5fZ-ciZoJtWdqR768n9EObrBVRBsO05CHMFXBVXMMGYep6taqR5wrXDLaXLgU_Gec8o-br12bsK_G9jDh6eL7N2pX60OPxxyLcgwe7eLb6NdqGOcyygWfg19HjA_IPdf6hA9vzwvy8dXLD5dv6NW7128vX1xRK1STqVJuv5fYQa9KTC55U2NvHdi-YVILLawQ2LIW605rp8pYOd31TmCnGtd04oI83XxLnk8LpmzGIVn0vp0wLMkUQy4kE1oW9Mk_6HVY4lS22yjNgDeF4htlY0gpojMl2djG1TAwp1rMVosp-5pftZhjET2-tV66Efs_kt89FEBsQDr90wHj37f_Y_sT4SadKQ</recordid><startdate>20210701</startdate><enddate>20210701</enddate><creator>He, Xiyan</creator><creator>Wang, Yuzhi</creator><creator>Li, Heqiong</creator><creator>Chen, Jing</creator><creator>Liu, Ziwei</creator><creator>Xu, Fangting</creator><creator>Zhou, Yigang</creator><general>Springer Vienna</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>K9.</scope><scope>7X8</scope></search><sort><creationdate>20210701</creationdate><title>Specific recognition of protein by deep eutectic solvent–based magnetic β-cyclodextrin molecularly imprinted polymer</title><author>He, Xiyan ; Wang, Yuzhi ; Li, Heqiong ; Chen, Jing ; Liu, Ziwei ; Xu, Fangting ; Zhou, Yigang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c375t-77f996eb0d702126254edcf0cd5168383c33ea1ae4b88f7cd57f8bdf3eb75f5b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Adsorption</topic><topic>Analytical Chemistry</topic><topic>Animals</topic><topic>beta-Cyclodextrins - metabolism</topic><topic>Binary mixtures</topic><topic>Cattle</topic><topic>Characterization and Evaluation of Materials</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Cyclodextrins</topic><topic>Deep Eutectic Solvents - chemistry</topic><topic>Dichroism</topic><topic>Hemoglobin</topic><topic>Humans</topic><topic>Imprinted polymers</topic><topic>Microengineering</topic><topic>Molecularly Imprinted Polymers - metabolism</topic><topic>Nanochemistry</topic><topic>Nanotechnology</topic><topic>Original Paper</topic><topic>pH control</topic><topic>Recognition</topic><topic>Serum albumin</topic><topic>Solvents</topic><topic>Solvents - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>He, Xiyan</creatorcontrib><creatorcontrib>Wang, Yuzhi</creatorcontrib><creatorcontrib>Li, Heqiong</creatorcontrib><creatorcontrib>Chen, Jing</creatorcontrib><creatorcontrib>Liu, Ziwei</creatorcontrib><creatorcontrib>Xu, Fangting</creatorcontrib><creatorcontrib>Zhou, Yigang</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>Mikrochimica acta (1966)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>He, Xiyan</au><au>Wang, Yuzhi</au><au>Li, Heqiong</au><au>Chen, Jing</au><au>Liu, Ziwei</au><au>Xu, Fangting</au><au>Zhou, Yigang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Specific recognition of protein by deep eutectic solvent–based magnetic β-cyclodextrin molecularly imprinted polymer</atitle><jtitle>Mikrochimica acta (1966)</jtitle><stitle>Microchim Acta</stitle><addtitle>Mikrochim Acta</addtitle><date>2021-07-01</date><risdate>2021</risdate><volume>188</volume><issue>7</issue><spage>232</spage><epage>232</epage><pages>232-232</pages><artnum>232</artnum><issn>0026-3672</issn><eissn>1436-5073</eissn><abstract>A magnetic β-cyclodextrin (MCD) surface molecularly imprinted polymer (MIP) based on deep eutectic solvents (DESs) as cross-linker and functional monomer (MCD@DES-MIP) was successfully synthesized for the specific recognition of bovine hemoglobin (BHb). The adsorption behavior of MCD@DES-MIP for BHb was investigated by adsorption thermodynamics, adsorption kinetics, and pH control experiments. The maximum adsorption capacity of MCD@DES-MIP for BHb under the optimized conditions was 195.94 mg g
−1
and the imprinting factor was 4.68. In addition, the competitive adsorption experiments demonstrated that MCD@DES-MIP showed excellent selective extraction ability for BHb in the binary mixture of BHb and bovine serum albumin (BSA). The actual sample analysis manifested that MCD@DES-MIP effectively separated BHb from complex samples. The results of circular dichroism spectra proved that the secondary structure of BHb did not change during elution. The result indicated that MCD@DES-MIP can be used as a new imprinting material for the separation and purification of BHb.
Graphical abstract
Magnetic imprinted microspheres (MCD@DES-MIP) were prepared by free radical polymerization using magnetic β-cyclodextrin (MCD) as carrier, deep eutectic solvents (DESs) as functional monomer and cross-linker. MCD@DES-MIP show high adsorption capacity and excellent selectivity for BHb.</abstract><cop>Vienna</cop><pub>Springer Vienna</pub><pmid>34137917</pmid><doi>10.1007/s00604-021-04887-x</doi><tpages>1</tpages></addata></record> |
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| subjects | Adsorption Analytical Chemistry Animals beta-Cyclodextrins - metabolism Binary mixtures Cattle Characterization and Evaluation of Materials Chemistry Chemistry and Materials Science Cyclodextrins Deep Eutectic Solvents - chemistry Dichroism Hemoglobin Humans Imprinted polymers Microengineering Molecularly Imprinted Polymers - metabolism Nanochemistry Nanotechnology Original Paper pH control Recognition Serum albumin Solvents Solvents - chemistry |
| title | Specific recognition of protein by deep eutectic solvent–based magnetic β-cyclodextrin molecularly imprinted polymer |
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