Microscopic Detection, Hematological Evaluation and Molecular Characterization of Piroplasms from Naturally Infected Dogs in Rio de Janeiro, Brazil
Purpose This study aimed to analyze the frequency of piroplasmids in the blood of dogs in Rio de Janeiro, compare the performance of microscopic techniques, assess the risk factors associated with infections and also molecularly and morphologically characterize the piroplasmids identified. Methods I...
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| Veröffentlicht in: | Acta parasitologica 2021-12, Vol.66 (4), p.1548-1560 |
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| creator | dos Santos, Fernanda Barbosa Gazêta, Gilberto Salles Corrêa, Laís Lisboa Lobão, Lucas Fernandes Palmer, João Pedro Siqueira Dib, Laís Verdan Damasceno, José André Lessa Moura-Martiniano, Nicole Oliveira Bastos, Otilio Machado Pereira Uchôa, Claudia Maria Antunes da Silva Barbosa, Alynne |
| description | Purpose
This study aimed to analyze the frequency of piroplasmids in the blood of dogs in Rio de Janeiro, compare the performance of microscopic techniques, assess the risk factors associated with infections and also molecularly and morphologically characterize the piroplasmids identified.
Methods
In all, 407 blood samples were collected from dogs between 2018 and 2019. These were subjected to microscopic parasitological techniques for thin and thick smears, stained with Giemsa and using a rapid staining kit. The slides were read under an optical microscope and the protozoa were characterized morphometrically. In addition, the blood samples were subjected to molecular characterization for diagnosing piroplasmid species using primers that amplified the gene 18S rRNA.
Results
Piroplasmids were detected in 38 (9.3%) samples. Of these, 33 samples presented nucleotide sequences compatible with
Babesia vogeli.
Most of the positive samples were young, male, defined breeds dogs that had been attended in clinics in São Gonçalo city. Thrombocytopenia and leukopenia were the hematological alterations more observed in positive samples, but positive samples without alterations were also detected. The sex was the only variable that showed statistical differences. Males dogs being more often infected than females (
p
|
| doi_str_mv | 10.1007/s11686-021-00426-z |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2541323063</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2541323063</sourcerecordid><originalsourceid>FETCH-LOGICAL-c396t-e1edfe6aec9bbe1860ef7ceab090ac9cc67a9cc89d41d1218979a68f5182330f3</originalsourceid><addsrcrecordid>eNp9kc1qGzEUhYfSQNwkL5CVoJsuPK1-xrJm2TpJk-D8EJK1uNZcuTKakSvNBOLX6AtX7hQCWWSjK7jfOVzOKYpTRr8ySuffEmNSyZJyVlJacVnuPhQTpmpZMjVjH_OfC1pyxdlh8SmlTYakUmpS_LlxJoZkwtYZcoY9mt6FbkousYU--LB2Bjw5fwY_wH5DoGvITfBoBg-RLH5BBNNjdLtxHSy5dzFsPaQ2ERtDS26hHyJ4_0KuOpv9sSFnYZ2I68iDC6RBcg0dZtGU_Iiwc_64OLDgE578n0fF08X54-KyXN79vFp8X5ZG1LIvkWFjUQKaerVCpiRFOzcIK1pTMLUxcg75VXVTsYbxnMa8BqnsjCkuBLXiqPgy-m5j-D1g6nXrkkHv8zlhSJrPKiZyblJk9PMbdBOG2OXrNJe0qhSfiSpTfKT2kaaIVm-jayG-aEb1vic99qRzT_pfT3qXRWIUpQx3a4yv1u-o_gIOiJkK</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2604482534</pqid></control><display><type>article</type><title>Microscopic Detection, Hematological Evaluation and Molecular Characterization of Piroplasms from Naturally Infected Dogs in Rio de Janeiro, Brazil</title><source>SpringerLink Journals</source><creator>dos Santos, Fernanda Barbosa ; Gazêta, Gilberto Salles ; Corrêa, Laís Lisboa ; Lobão, Lucas Fernandes ; Palmer, João Pedro Siqueira ; Dib, Laís Verdan ; Damasceno, José André Lessa ; Moura-Martiniano, Nicole Oliveira ; Bastos, Otilio Machado Pereira ; Uchôa, Claudia Maria Antunes ; da Silva Barbosa, Alynne</creator><creatorcontrib>dos Santos, Fernanda Barbosa ; Gazêta, Gilberto Salles ; Corrêa, Laís Lisboa ; Lobão, Lucas Fernandes ; Palmer, João Pedro Siqueira ; Dib, Laís Verdan ; Damasceno, José André Lessa ; Moura-Martiniano, Nicole Oliveira ; Bastos, Otilio Machado Pereira ; Uchôa, Claudia Maria Antunes ; da Silva Barbosa, Alynne</creatorcontrib><description>Purpose
This study aimed to analyze the frequency of piroplasmids in the blood of dogs in Rio de Janeiro, compare the performance of microscopic techniques, assess the risk factors associated with infections and also molecularly and morphologically characterize the piroplasmids identified.
Methods
In all, 407 blood samples were collected from dogs between 2018 and 2019. These were subjected to microscopic parasitological techniques for thin and thick smears, stained with Giemsa and using a rapid staining kit. The slides were read under an optical microscope and the protozoa were characterized morphometrically. In addition, the blood samples were subjected to molecular characterization for diagnosing piroplasmid species using primers that amplified the gene 18S rRNA.
Results
Piroplasmids were detected in 38 (9.3%) samples. Of these, 33 samples presented nucleotide sequences compatible with
Babesia vogeli.
Most of the positive samples were young, male, defined breeds dogs that had been attended in clinics in São Gonçalo city. Thrombocytopenia and leukopenia were the hematological alterations more observed in positive samples, but positive samples without alterations were also detected. The sex was the only variable that showed statistical differences. Males dogs being more often infected than females (
p
< 0.05). The microscope slides mostly showed piriform and oval merozoites measuring greater than 2.5 µm in length, which were compatible with
B. vogeli
. However, smaller forms were also identified, thus demonstrating the polymorphic nature of this parasite.
Conclusion
Babesia vogeli
was detected in blood samples from dogs in the metropolitan cities of Rio de Janeiro by molecular techniques in different parasite morphotypes.</description><identifier>ISSN: 1230-2821</identifier><identifier>EISSN: 1896-1851</identifier><identifier>DOI: 10.1007/s11686-021-00426-z</identifier><language>eng</language><publisher>Cham: Springer International Publishing</publisher><subject>Animal Systematics/Taxonomy/Biogeography ; Babesia canis vogeli ; Biomedical and Life Sciences ; Biomedicine ; Blood ; Dogs ; Ecology ; Frequency analysis ; Health risks ; Hematology ; Leukopenia ; Medical Microbiology ; Merozoites ; Microbiology ; Nucleotides ; Optical microscopes ; Original Paper ; Parasites ; Parasitology ; Protozoa ; Risk analysis ; Risk factors ; rRNA 18S ; Statistical methods ; Thrombocytopenia</subject><ispartof>Acta parasitologica, 2021-12, Vol.66 (4), p.1548-1560</ispartof><rights>Witold Stefański Institute of Parasitology, Polish Academy of Sciences 2021</rights><rights>Witold Stefański Institute of Parasitology, Polish Academy of Sciences 2021.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c396t-e1edfe6aec9bbe1860ef7ceab090ac9cc67a9cc89d41d1218979a68f5182330f3</citedby><cites>FETCH-LOGICAL-c396t-e1edfe6aec9bbe1860ef7ceab090ac9cc67a9cc89d41d1218979a68f5182330f3</cites><orcidid>0000-0002-5007-1339</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11686-021-00426-z$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11686-021-00426-z$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids></links><search><creatorcontrib>dos Santos, Fernanda Barbosa</creatorcontrib><creatorcontrib>Gazêta, Gilberto Salles</creatorcontrib><creatorcontrib>Corrêa, Laís Lisboa</creatorcontrib><creatorcontrib>Lobão, Lucas Fernandes</creatorcontrib><creatorcontrib>Palmer, João Pedro Siqueira</creatorcontrib><creatorcontrib>Dib, Laís Verdan</creatorcontrib><creatorcontrib>Damasceno, José André Lessa</creatorcontrib><creatorcontrib>Moura-Martiniano, Nicole Oliveira</creatorcontrib><creatorcontrib>Bastos, Otilio Machado Pereira</creatorcontrib><creatorcontrib>Uchôa, Claudia Maria Antunes</creatorcontrib><creatorcontrib>da Silva Barbosa, Alynne</creatorcontrib><title>Microscopic Detection, Hematological Evaluation and Molecular Characterization of Piroplasms from Naturally Infected Dogs in Rio de Janeiro, Brazil</title><title>Acta parasitologica</title><addtitle>Acta Parasit</addtitle><description>Purpose
This study aimed to analyze the frequency of piroplasmids in the blood of dogs in Rio de Janeiro, compare the performance of microscopic techniques, assess the risk factors associated with infections and also molecularly and morphologically characterize the piroplasmids identified.
Methods
In all, 407 blood samples were collected from dogs between 2018 and 2019. These were subjected to microscopic parasitological techniques for thin and thick smears, stained with Giemsa and using a rapid staining kit. The slides were read under an optical microscope and the protozoa were characterized morphometrically. In addition, the blood samples were subjected to molecular characterization for diagnosing piroplasmid species using primers that amplified the gene 18S rRNA.
Results
Piroplasmids were detected in 38 (9.3%) samples. Of these, 33 samples presented nucleotide sequences compatible with
Babesia vogeli.
Most of the positive samples were young, male, defined breeds dogs that had been attended in clinics in São Gonçalo city. Thrombocytopenia and leukopenia were the hematological alterations more observed in positive samples, but positive samples without alterations were also detected. The sex was the only variable that showed statistical differences. Males dogs being more often infected than females (
p
< 0.05). The microscope slides mostly showed piriform and oval merozoites measuring greater than 2.5 µm in length, which were compatible with
B. vogeli
. However, smaller forms were also identified, thus demonstrating the polymorphic nature of this parasite.
Conclusion
Babesia vogeli
was detected in blood samples from dogs in the metropolitan cities of Rio de Janeiro by molecular techniques in different parasite morphotypes.</description><subject>Animal Systematics/Taxonomy/Biogeography</subject><subject>Babesia canis vogeli</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Blood</subject><subject>Dogs</subject><subject>Ecology</subject><subject>Frequency analysis</subject><subject>Health risks</subject><subject>Hematology</subject><subject>Leukopenia</subject><subject>Medical Microbiology</subject><subject>Merozoites</subject><subject>Microbiology</subject><subject>Nucleotides</subject><subject>Optical microscopes</subject><subject>Original Paper</subject><subject>Parasites</subject><subject>Parasitology</subject><subject>Protozoa</subject><subject>Risk analysis</subject><subject>Risk factors</subject><subject>rRNA 18S</subject><subject>Statistical methods</subject><subject>Thrombocytopenia</subject><issn>1230-2821</issn><issn>1896-1851</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNp9kc1qGzEUhYfSQNwkL5CVoJsuPK1-xrJm2TpJk-D8EJK1uNZcuTKakSvNBOLX6AtX7hQCWWSjK7jfOVzOKYpTRr8ySuffEmNSyZJyVlJacVnuPhQTpmpZMjVjH_OfC1pyxdlh8SmlTYakUmpS_LlxJoZkwtYZcoY9mt6FbkousYU--LB2Bjw5fwY_wH5DoGvITfBoBg-RLH5BBNNjdLtxHSy5dzFsPaQ2ERtDS26hHyJ4_0KuOpv9sSFnYZ2I68iDC6RBcg0dZtGU_Iiwc_64OLDgE578n0fF08X54-KyXN79vFp8X5ZG1LIvkWFjUQKaerVCpiRFOzcIK1pTMLUxcg75VXVTsYbxnMa8BqnsjCkuBLXiqPgy-m5j-D1g6nXrkkHv8zlhSJrPKiZyblJk9PMbdBOG2OXrNJe0qhSfiSpTfKT2kaaIVm-jayG-aEb1vic99qRzT_pfT3qXRWIUpQx3a4yv1u-o_gIOiJkK</recordid><startdate>20211201</startdate><enddate>20211201</enddate><creator>dos Santos, Fernanda Barbosa</creator><creator>Gazêta, Gilberto Salles</creator><creator>Corrêa, Laís Lisboa</creator><creator>Lobão, Lucas Fernandes</creator><creator>Palmer, João Pedro Siqueira</creator><creator>Dib, Laís Verdan</creator><creator>Damasceno, José André Lessa</creator><creator>Moura-Martiniano, Nicole Oliveira</creator><creator>Bastos, Otilio Machado Pereira</creator><creator>Uchôa, Claudia Maria Antunes</creator><creator>da Silva Barbosa, Alynne</creator><general>Springer International Publishing</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QL</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7T7</scope><scope>7TM</scope><scope>7U7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-5007-1339</orcidid></search><sort><creationdate>20211201</creationdate><title>Microscopic Detection, Hematological Evaluation and Molecular Characterization of Piroplasms from Naturally Infected Dogs in Rio de Janeiro, Brazil</title><author>dos Santos, Fernanda Barbosa ; Gazêta, Gilberto Salles ; Corrêa, Laís Lisboa ; Lobão, Lucas Fernandes ; Palmer, João Pedro Siqueira ; Dib, Laís Verdan ; Damasceno, José André Lessa ; Moura-Martiniano, Nicole Oliveira ; Bastos, Otilio Machado Pereira ; Uchôa, Claudia Maria Antunes ; da Silva Barbosa, Alynne</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c396t-e1edfe6aec9bbe1860ef7ceab090ac9cc67a9cc89d41d1218979a68f5182330f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Animal Systematics/Taxonomy/Biogeography</topic><topic>Babesia canis vogeli</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Blood</topic><topic>Dogs</topic><topic>Ecology</topic><topic>Frequency analysis</topic><topic>Health risks</topic><topic>Hematology</topic><topic>Leukopenia</topic><topic>Medical Microbiology</topic><topic>Merozoites</topic><topic>Microbiology</topic><topic>Nucleotides</topic><topic>Optical microscopes</topic><topic>Original Paper</topic><topic>Parasites</topic><topic>Parasitology</topic><topic>Protozoa</topic><topic>Risk analysis</topic><topic>Risk factors</topic><topic>rRNA 18S</topic><topic>Statistical methods</topic><topic>Thrombocytopenia</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>dos Santos, Fernanda Barbosa</creatorcontrib><creatorcontrib>Gazêta, Gilberto Salles</creatorcontrib><creatorcontrib>Corrêa, Laís Lisboa</creatorcontrib><creatorcontrib>Lobão, Lucas Fernandes</creatorcontrib><creatorcontrib>Palmer, João Pedro Siqueira</creatorcontrib><creatorcontrib>Dib, Laís Verdan</creatorcontrib><creatorcontrib>Damasceno, José André Lessa</creatorcontrib><creatorcontrib>Moura-Martiniano, Nicole Oliveira</creatorcontrib><creatorcontrib>Bastos, Otilio Machado Pereira</creatorcontrib><creatorcontrib>Uchôa, Claudia Maria Antunes</creatorcontrib><creatorcontrib>da Silva Barbosa, Alynne</creatorcontrib><collection>CrossRef</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Acta parasitologica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>dos Santos, Fernanda Barbosa</au><au>Gazêta, Gilberto Salles</au><au>Corrêa, Laís Lisboa</au><au>Lobão, Lucas Fernandes</au><au>Palmer, João Pedro Siqueira</au><au>Dib, Laís Verdan</au><au>Damasceno, José André Lessa</au><au>Moura-Martiniano, Nicole Oliveira</au><au>Bastos, Otilio Machado Pereira</au><au>Uchôa, Claudia Maria Antunes</au><au>da Silva Barbosa, Alynne</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Microscopic Detection, Hematological Evaluation and Molecular Characterization of Piroplasms from Naturally Infected Dogs in Rio de Janeiro, Brazil</atitle><jtitle>Acta parasitologica</jtitle><stitle>Acta Parasit</stitle><date>2021-12-01</date><risdate>2021</risdate><volume>66</volume><issue>4</issue><spage>1548</spage><epage>1560</epage><pages>1548-1560</pages><issn>1230-2821</issn><eissn>1896-1851</eissn><abstract>Purpose
This study aimed to analyze the frequency of piroplasmids in the blood of dogs in Rio de Janeiro, compare the performance of microscopic techniques, assess the risk factors associated with infections and also molecularly and morphologically characterize the piroplasmids identified.
Methods
In all, 407 blood samples were collected from dogs between 2018 and 2019. These were subjected to microscopic parasitological techniques for thin and thick smears, stained with Giemsa and using a rapid staining kit. The slides were read under an optical microscope and the protozoa were characterized morphometrically. In addition, the blood samples were subjected to molecular characterization for diagnosing piroplasmid species using primers that amplified the gene 18S rRNA.
Results
Piroplasmids were detected in 38 (9.3%) samples. Of these, 33 samples presented nucleotide sequences compatible with
Babesia vogeli.
Most of the positive samples were young, male, defined breeds dogs that had been attended in clinics in São Gonçalo city. Thrombocytopenia and leukopenia were the hematological alterations more observed in positive samples, but positive samples without alterations were also detected. The sex was the only variable that showed statistical differences. Males dogs being more often infected than females (
p
< 0.05). The microscope slides mostly showed piriform and oval merozoites measuring greater than 2.5 µm in length, which were compatible with
B. vogeli
. However, smaller forms were also identified, thus demonstrating the polymorphic nature of this parasite.
Conclusion
Babesia vogeli
was detected in blood samples from dogs in the metropolitan cities of Rio de Janeiro by molecular techniques in different parasite morphotypes.</abstract><cop>Cham</cop><pub>Springer International Publishing</pub><doi>10.1007/s11686-021-00426-z</doi><tpages>13</tpages><orcidid>https://orcid.org/0000-0002-5007-1339</orcidid><oa>free_for_read</oa></addata></record> |
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| language | eng |
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| subjects | Animal Systematics/Taxonomy/Biogeography Babesia canis vogeli Biomedical and Life Sciences Biomedicine Blood Dogs Ecology Frequency analysis Health risks Hematology Leukopenia Medical Microbiology Merozoites Microbiology Nucleotides Optical microscopes Original Paper Parasites Parasitology Protozoa Risk analysis Risk factors rRNA 18S Statistical methods Thrombocytopenia |
| title | Microscopic Detection, Hematological Evaluation and Molecular Characterization of Piroplasms from Naturally Infected Dogs in Rio de Janeiro, Brazil |
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