The effect of dilution rate and successive semen collections on sperm quality and sexual motivation of sexually mature South African Merino rams
This study investigated whether dilution rate and successive semen collections influenced sperm viability, morphology, motility and male sexual motivation in sexually mature South African Merino rams (SAMR). Semen was collected from 11 rams up to either sperm or behavioural exhaustion. Semen was the...
Gespeichert in:
| Veröffentlicht in: | Tropical animal health and production 2021-03, Vol.53 (1), p.182-182, Article 182 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 182 |
|---|---|
| container_issue | 1 |
| container_start_page | 182 |
| container_title | Tropical animal health and production |
| container_volume | 53 |
| creator | Bonato, Maud Smith, Marna A. M. J. Malecki, Irek A. Cloete, Schalk W. P. |
| description | This study investigated whether dilution rate and successive semen collections influenced sperm viability, morphology, motility and male sexual motivation in sexually mature South African Merino rams (SAMR). Semen was collected from 11 rams up to either sperm or behavioural exhaustion. Semen was then immediately serial diluted to make 0, 2, 4, 8 and 16× dilutions with Hams F10 diluent. Following dilution, sperm motility was evaluated using computer-assisted sperm analysis (SCA®), while sperm morphology and viability were assessed using nigrosin–eosin staining and SYBR14/PI, respectively. Male sexual motivation was recorded by reaction time to first mount, while male dexterity was calculated as the ratio of mounts to ejaculations. An increase of dilution rate did not affect sperm motility (
P
> 0.05) but resulted in a significant decrease in the percentage of live normal sperm (
P
< 0.05). Furthermore, while sperm concentration and number decreased with semen collection number (
P
< 0.05), no effect was detected on sperm viability, morphology and motility (
P
> 0.05), except for average curvilinear velocity which showed a biphasic trend (
P
< 0.05). Mating success and reaction time were negatively affected by successive semen collections (
P
< 0.05), while male dexterity was unaffected (
P
> 0.05). Nevertheless, relatively high numbers of motile sperm (>2 billion) were collected up to the 4th successive semen collection, with a short initial reaction period (65%). SAMR thus withstood frequent semen collections without affecting their sperm reserves or sexual motivation. Further studies are required to investigate optimal conditions for semen collection and artificial insemination in this breed. |
| doi_str_mv | 10.1007/s11250-021-02627-0 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2540493089</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2529016754</sourcerecordid><originalsourceid>FETCH-LOGICAL-c436t-f1125a39a401a6c89ad33abf59667980f0091961b6b8c184c820ce95112808b13</originalsourceid><addsrcrecordid>eNqFks1u1TAQhS0EopfCC7BAltiwCYx_4p9lVUFBKmJBWUeO75i6SuJbO6l634JHrm9TQGJRFpbl8XfOaOxDyGsG7xmA_lAY4y00wFldiusGnpANa7VotBTmKdkASNtILfUReVHKFUCVGfWcHAmhhBS83ZBfF5dIMQT0M02BbuOwzDFNNLsZqZu2tCzeYynxBmnBESfq0zBUukKFVrDsMI_0enFDnPerAm_riY5pjjfu3qwar8VhT0c3Lxnp97TMl_Qk5OjdRL9ijlOqTcfykjwLbij46mE_Jj8-fbw4_dycfzv7cnpy3ngp1NyEw-xOWCeBOeWNdVshXB9aq5S2BgKAZVaxXvXGMyO94eDRtlVmwPRMHJN3q-8up-sFy9yNsXgcBjdhWkrHW1lfT4Cx_0elldxqMKKib_9Br9KSpzpINeQWmNKtfJSqPXlrGD9QfKV8TqVkDN0ux9HlfcegOwSgWwPQ1QB09wHooIrePFgv_YjbP5LfP14BsQKlXk0_Mf_t_YjtHQAIuqA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2493258124</pqid></control><display><type>article</type><title>The effect of dilution rate and successive semen collections on sperm quality and sexual motivation of sexually mature South African Merino rams</title><source>SpringerLink Journals</source><creator>Bonato, Maud ; Smith, Marna A. M. J. ; Malecki, Irek A. ; Cloete, Schalk W. P.</creator><creatorcontrib>Bonato, Maud ; Smith, Marna A. M. J. ; Malecki, Irek A. ; Cloete, Schalk W. P.</creatorcontrib><description>This study investigated whether dilution rate and successive semen collections influenced sperm viability, morphology, motility and male sexual motivation in sexually mature South African Merino rams (SAMR). Semen was collected from 11 rams up to either sperm or behavioural exhaustion. Semen was then immediately serial diluted to make 0, 2, 4, 8 and 16× dilutions with Hams F10 diluent. Following dilution, sperm motility was evaluated using computer-assisted sperm analysis (SCA®), while sperm morphology and viability were assessed using nigrosin–eosin staining and SYBR14/PI, respectively. Male sexual motivation was recorded by reaction time to first mount, while male dexterity was calculated as the ratio of mounts to ejaculations. An increase of dilution rate did not affect sperm motility (
P
> 0.05) but resulted in a significant decrease in the percentage of live normal sperm (
P
< 0.05). Furthermore, while sperm concentration and number decreased with semen collection number (
P
< 0.05), no effect was detected on sperm viability, morphology and motility (
P
> 0.05), except for average curvilinear velocity which showed a biphasic trend (
P
< 0.05). Mating success and reaction time were negatively affected by successive semen collections (
P
< 0.05), while male dexterity was unaffected (
P
> 0.05). Nevertheless, relatively high numbers of motile sperm (>2 billion) were collected up to the 4th successive semen collection, with a short initial reaction period (<25 s) and good success rate (>65%). SAMR thus withstood frequent semen collections without affecting their sperm reserves or sexual motivation. Further studies are required to investigate optimal conditions for semen collection and artificial insemination in this breed.</description><identifier>ISSN: 0049-4747</identifier><identifier>EISSN: 1573-7438</identifier><identifier>DOI: 10.1007/s11250-021-02627-0</identifier><identifier>PMID: 33634325</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Artificial insemination ; Biomedical and Life Sciences ; Collection ; Collections ; Dilution ; Life Sciences ; Males ; Merino ; Morphology ; Motility ; Motivation ; Rams ; Reaction time ; Regular Articles ; Reproduction (biology) ; Semen ; sexual maturity ; Sperm ; sperm concentration ; sperm motility ; sperm quality ; Veterinary Medicine/Veterinary Science ; Viability ; Zoology</subject><ispartof>Tropical animal health and production, 2021-03, Vol.53 (1), p.182-182, Article 182</ispartof><rights>The Author(s), under exclusive licence to Springer Nature B.V. part of Springer Nature 2021</rights><rights>The Author(s), under exclusive licence to Springer Nature B.V. part of Springer Nature 2021.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c436t-f1125a39a401a6c89ad33abf59667980f0091961b6b8c184c820ce95112808b13</citedby><cites>FETCH-LOGICAL-c436t-f1125a39a401a6c89ad33abf59667980f0091961b6b8c184c820ce95112808b13</cites><orcidid>0000-0002-0790-0859</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11250-021-02627-0$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11250-021-02627-0$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33634325$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bonato, Maud</creatorcontrib><creatorcontrib>Smith, Marna A. M. J.</creatorcontrib><creatorcontrib>Malecki, Irek A.</creatorcontrib><creatorcontrib>Cloete, Schalk W. P.</creatorcontrib><title>The effect of dilution rate and successive semen collections on sperm quality and sexual motivation of sexually mature South African Merino rams</title><title>Tropical animal health and production</title><addtitle>Trop Anim Health Prod</addtitle><addtitle>Trop Anim Health Prod</addtitle><description>This study investigated whether dilution rate and successive semen collections influenced sperm viability, morphology, motility and male sexual motivation in sexually mature South African Merino rams (SAMR). Semen was collected from 11 rams up to either sperm or behavioural exhaustion. Semen was then immediately serial diluted to make 0, 2, 4, 8 and 16× dilutions with Hams F10 diluent. Following dilution, sperm motility was evaluated using computer-assisted sperm analysis (SCA®), while sperm morphology and viability were assessed using nigrosin–eosin staining and SYBR14/PI, respectively. Male sexual motivation was recorded by reaction time to first mount, while male dexterity was calculated as the ratio of mounts to ejaculations. An increase of dilution rate did not affect sperm motility (
P
> 0.05) but resulted in a significant decrease in the percentage of live normal sperm (
P
< 0.05). Furthermore, while sperm concentration and number decreased with semen collection number (
P
< 0.05), no effect was detected on sperm viability, morphology and motility (
P
> 0.05), except for average curvilinear velocity which showed a biphasic trend (
P
< 0.05). Mating success and reaction time were negatively affected by successive semen collections (
P
< 0.05), while male dexterity was unaffected (
P
> 0.05). Nevertheless, relatively high numbers of motile sperm (>2 billion) were collected up to the 4th successive semen collection, with a short initial reaction period (<25 s) and good success rate (>65%). SAMR thus withstood frequent semen collections without affecting their sperm reserves or sexual motivation. Further studies are required to investigate optimal conditions for semen collection and artificial insemination in this breed.</description><subject>Artificial insemination</subject><subject>Biomedical and Life Sciences</subject><subject>Collection</subject><subject>Collections</subject><subject>Dilution</subject><subject>Life Sciences</subject><subject>Males</subject><subject>Merino</subject><subject>Morphology</subject><subject>Motility</subject><subject>Motivation</subject><subject>Rams</subject><subject>Reaction time</subject><subject>Regular Articles</subject><subject>Reproduction (biology)</subject><subject>Semen</subject><subject>sexual maturity</subject><subject>Sperm</subject><subject>sperm concentration</subject><subject>sperm motility</subject><subject>sperm quality</subject><subject>Veterinary Medicine/Veterinary Science</subject><subject>Viability</subject><subject>Zoology</subject><issn>0049-4747</issn><issn>1573-7438</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNqFks1u1TAQhS0EopfCC7BAltiwCYx_4p9lVUFBKmJBWUeO75i6SuJbO6l634JHrm9TQGJRFpbl8XfOaOxDyGsG7xmA_lAY4y00wFldiusGnpANa7VotBTmKdkASNtILfUReVHKFUCVGfWcHAmhhBS83ZBfF5dIMQT0M02BbuOwzDFNNLsZqZu2tCzeYynxBmnBESfq0zBUukKFVrDsMI_0enFDnPerAm_riY5pjjfu3qwar8VhT0c3Lxnp97TMl_Qk5OjdRL9ijlOqTcfykjwLbij46mE_Jj8-fbw4_dycfzv7cnpy3ngp1NyEw-xOWCeBOeWNdVshXB9aq5S2BgKAZVaxXvXGMyO94eDRtlVmwPRMHJN3q-8up-sFy9yNsXgcBjdhWkrHW1lfT4Cx_0elldxqMKKib_9Br9KSpzpINeQWmNKtfJSqPXlrGD9QfKV8TqVkDN0ux9HlfcegOwSgWwPQ1QB09wHooIrePFgv_YjbP5LfP14BsQKlXk0_Mf_t_YjtHQAIuqA</recordid><startdate>20210301</startdate><enddate>20210301</enddate><creator>Bonato, Maud</creator><creator>Smith, Marna A. M. J.</creator><creator>Malecki, Irek A.</creator><creator>Cloete, Schalk W. P.</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7U7</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope><orcidid>https://orcid.org/0000-0002-0790-0859</orcidid></search><sort><creationdate>20210301</creationdate><title>The effect of dilution rate and successive semen collections on sperm quality and sexual motivation of sexually mature South African Merino rams</title><author>Bonato, Maud ; Smith, Marna A. M. J. ; Malecki, Irek A. ; Cloete, Schalk W. P.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c436t-f1125a39a401a6c89ad33abf59667980f0091961b6b8c184c820ce95112808b13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Artificial insemination</topic><topic>Biomedical and Life Sciences</topic><topic>Collection</topic><topic>Collections</topic><topic>Dilution</topic><topic>Life Sciences</topic><topic>Males</topic><topic>Merino</topic><topic>Morphology</topic><topic>Motility</topic><topic>Motivation</topic><topic>Rams</topic><topic>Reaction time</topic><topic>Regular Articles</topic><topic>Reproduction (biology)</topic><topic>Semen</topic><topic>sexual maturity</topic><topic>Sperm</topic><topic>sperm concentration</topic><topic>sperm motility</topic><topic>sperm quality</topic><topic>Veterinary Medicine/Veterinary Science</topic><topic>Viability</topic><topic>Zoology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bonato, Maud</creatorcontrib><creatorcontrib>Smith, Marna A. M. J.</creatorcontrib><creatorcontrib>Malecki, Irek A.</creatorcontrib><creatorcontrib>Cloete, Schalk W. P.</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>Tropical animal health and production</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bonato, Maud</au><au>Smith, Marna A. M. J.</au><au>Malecki, Irek A.</au><au>Cloete, Schalk W. P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The effect of dilution rate and successive semen collections on sperm quality and sexual motivation of sexually mature South African Merino rams</atitle><jtitle>Tropical animal health and production</jtitle><stitle>Trop Anim Health Prod</stitle><addtitle>Trop Anim Health Prod</addtitle><date>2021-03-01</date><risdate>2021</risdate><volume>53</volume><issue>1</issue><spage>182</spage><epage>182</epage><pages>182-182</pages><artnum>182</artnum><issn>0049-4747</issn><eissn>1573-7438</eissn><abstract>This study investigated whether dilution rate and successive semen collections influenced sperm viability, morphology, motility and male sexual motivation in sexually mature South African Merino rams (SAMR). Semen was collected from 11 rams up to either sperm or behavioural exhaustion. Semen was then immediately serial diluted to make 0, 2, 4, 8 and 16× dilutions with Hams F10 diluent. Following dilution, sperm motility was evaluated using computer-assisted sperm analysis (SCA®), while sperm morphology and viability were assessed using nigrosin–eosin staining and SYBR14/PI, respectively. Male sexual motivation was recorded by reaction time to first mount, while male dexterity was calculated as the ratio of mounts to ejaculations. An increase of dilution rate did not affect sperm motility (
P
> 0.05) but resulted in a significant decrease in the percentage of live normal sperm (
P
< 0.05). Furthermore, while sperm concentration and number decreased with semen collection number (
P
< 0.05), no effect was detected on sperm viability, morphology and motility (
P
> 0.05), except for average curvilinear velocity which showed a biphasic trend (
P
< 0.05). Mating success and reaction time were negatively affected by successive semen collections (
P
< 0.05), while male dexterity was unaffected (
P
> 0.05). Nevertheless, relatively high numbers of motile sperm (>2 billion) were collected up to the 4th successive semen collection, with a short initial reaction period (<25 s) and good success rate (>65%). SAMR thus withstood frequent semen collections without affecting their sperm reserves or sexual motivation. Further studies are required to investigate optimal conditions for semen collection and artificial insemination in this breed.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>33634325</pmid><doi>10.1007/s11250-021-02627-0</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0002-0790-0859</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0049-4747 |
| ispartof | Tropical animal health and production, 2021-03, Vol.53 (1), p.182-182, Article 182 |
| issn | 0049-4747 1573-7438 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2540493089 |
| source | SpringerLink Journals |
| subjects | Artificial insemination Biomedical and Life Sciences Collection Collections Dilution Life Sciences Males Merino Morphology Motility Motivation Rams Reaction time Regular Articles Reproduction (biology) Semen sexual maturity Sperm sperm concentration sperm motility sperm quality Veterinary Medicine/Veterinary Science Viability Zoology |
| title | The effect of dilution rate and successive semen collections on sperm quality and sexual motivation of sexually mature South African Merino rams |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-09T03%3A50%3A46IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=The%20effect%20of%20dilution%20rate%20and%20successive%20semen%20collections%20on%20sperm%20quality%20and%20sexual%20motivation%20of%20sexually%20mature%20South%20African%20Merino%20rams&rft.jtitle=Tropical%20animal%20health%20and%20production&rft.au=Bonato,%20Maud&rft.date=2021-03-01&rft.volume=53&rft.issue=1&rft.spage=182&rft.epage=182&rft.pages=182-182&rft.artnum=182&rft.issn=0049-4747&rft.eissn=1573-7438&rft_id=info:doi/10.1007/s11250-021-02627-0&rft_dat=%3Cproquest_cross%3E2529016754%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2493258124&rft_id=info:pmid/33634325&rfr_iscdi=true |