Accurate assessment of estrogen receptor profiles in non-functioning pituitary adenomas using RT-digital PCR and immunohistochemistry
Non-functioning pituitary adenomas (NFPAs) are common pituitary tumors, and surgery is generally the only treatment option. Few attempts have been made to explore target molecules for the development of NFPA pharmacological treatments. We quantitatively assessed the expression profiles of estrogen r...
Gespeichert in:
| Veröffentlicht in: | Life sciences (1973) 2020-11, Vol.260, p.118416-7, Article 118416 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 7 |
|---|---|
| container_issue | |
| container_start_page | 118416 |
| container_title | Life sciences (1973) |
| container_volume | 260 |
| creator | Hattori, Yujiro Ishii, Hirotaka Tahara, Shigeyuki Morita, Akio Ozawa, Hitoshi |
| description | Non-functioning pituitary adenomas (NFPAs) are common pituitary tumors, and surgery is generally the only treatment option. Few attempts have been made to explore target molecules for the development of NFPA pharmacological treatments.
We quantitatively assessed the expression profiles of estrogen receptor (ER) transcripts and proteins in NFPA samples, using reverse transcription-digital polymerase chain reaction (RT-dPCR) and immunohistochemistry, and further investigated the correlations between the expression levels of ER and those of downstream responsive genes. All patients had undergone surgery at the same high-volume hospital. A total of 20 patients with NFPAs were included. All patients were new-onset, and none were diagnosed with intratumoral hemorrhages or cysts.
NFPA samples exhibited a bimodal ESR1 expression pattern and were categorized into significantly different high- and low-ESR1 expression level groups (P |
| doi_str_mv | 10.1016/j.lfs.2020.118416 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2540489239</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0024320520311693</els_id><sourcerecordid>2442846583</sourcerecordid><originalsourceid>FETCH-LOGICAL-c457t-63425fe3f8183968796d7598ea87cdafcdeb2943cd2f97f96fe8d66066d519513</originalsourceid><addsrcrecordid>eNqFkc-KFDEQxoO44LjuA3gLePHSY_53Gk_L4KqwoCy75xCTymyG7mRM0sI-wL63GcaTBz0VRf2-Kr76EHpLyZYSqj4ctnOoW0ZY76kWVL1AG6rHaSCK05doQwgTA2dEvkKvaz0QQqQc-QY9Xzu3FtsA21qh1gVSwzlgqK3kPSRcwMGx5YKPJYc4Q8Ux4ZTTENbkWswppj0-xrbGZssTth5SXmzFaz0N7u4HH_d9NOPvuztsk8dxWdaUH2Nt2T3C0mt5eoMugp0rXP2pl-jh5tP97stw--3z19317eCEHNuguGAyAA-aaj6pbk_5UU4arB6dt8F5-MEmwZ1nYRrDpAJorxRRyks6Scov0fvz3m7m59o9mn7fwTzbBHmthklBhJ4Yn_6PCsG0UFLzjr77Cz3ktaRupFNKjpQIKjtFz5QrudYCwRxLXPrPDCXmlKE5mJ6hOWVozhl2zcezBvpTfkUoproIyYGPPZdmfI7_UP8GGlymDA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2465710415</pqid></control><display><type>article</type><title>Accurate assessment of estrogen receptor profiles in non-functioning pituitary adenomas using RT-digital PCR and immunohistochemistry</title><source>Elsevier ScienceDirect Journals</source><creator>Hattori, Yujiro ; Ishii, Hirotaka ; Tahara, Shigeyuki ; Morita, Akio ; Ozawa, Hitoshi</creator><creatorcontrib>Hattori, Yujiro ; Ishii, Hirotaka ; Tahara, Shigeyuki ; Morita, Akio ; Ozawa, Hitoshi</creatorcontrib><description>Non-functioning pituitary adenomas (NFPAs) are common pituitary tumors, and surgery is generally the only treatment option. Few attempts have been made to explore target molecules for the development of NFPA pharmacological treatments.
We quantitatively assessed the expression profiles of estrogen receptor (ER) transcripts and proteins in NFPA samples, using reverse transcription-digital polymerase chain reaction (RT-dPCR) and immunohistochemistry, and further investigated the correlations between the expression levels of ER and those of downstream responsive genes. All patients had undergone surgery at the same high-volume hospital. A total of 20 patients with NFPAs were included. All patients were new-onset, and none were diagnosed with intratumoral hemorrhages or cysts.
NFPA samples exhibited a bimodal ESR1 expression pattern and were categorized into significantly different high- and low-ESR1 expression level groups (P < 0.05). In contrast, expression levels of ESR1 variants and ESR2 could barely be detected. Similar results were obtained through the immunohistochemical staining of NFPAs, using well-validated antibodies against ERs. The expression levels of ESR1 positively correlated with those of GREB1, an estrogen-responsive gene [correlation coefficient (r) = 0.623, P = 0.003].
ESR1 expression levels in NFPAs exhibited a bimodal pattern and were positively correlated with GREB1 expression levels. The accurate assessment of ER expression levels may further advance future NFPA-related research.
•Non-functioning pituitary adenomas are currently only treatable through surgery.•We examined estrogen receptor gene expression profiles in NFPAs using RT-dPCR.•We examined the ER protein expression using well-validated antibodies.•NFPA samples exhibited a bimodal ESR1 expression pattern.•ESR1 expression levels in NFPAs were positively correlated with GREB1 expression.</description><identifier>ISSN: 0024-3205</identifier><identifier>EISSN: 1879-0631</identifier><identifier>DOI: 10.1016/j.lfs.2020.118416</identifier><language>eng</language><publisher>New York: Elsevier Inc</publisher><subject>Antibodies ; Brain tumors ; Correlation coefficient ; Correlation coefficients ; Cysts ; Drug therapy ; ESR1 ; ESR2 ; Estrogen receptors ; Estrogen response gene ; Estrogens ; genes ; GREB1 ; Hemorrhage ; hospitals ; Immunohistochemistry ; Non-functioning pituitary adenomas ; Patients ; Pituitary ; Polymerase chain reaction ; Receptors ; Reverse transcription ; RT-dPCR ; Surgery ; Tumors</subject><ispartof>Life sciences (1973), 2020-11, Vol.260, p.118416-7, Article 118416</ispartof><rights>2020 Elsevier Inc.</rights><rights>Copyright Elsevier BV Nov 2020</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c457t-63425fe3f8183968796d7598ea87cdafcdeb2943cd2f97f96fe8d66066d519513</citedby><cites>FETCH-LOGICAL-c457t-63425fe3f8183968796d7598ea87cdafcdeb2943cd2f97f96fe8d66066d519513</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0024320520311693$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids></links><search><creatorcontrib>Hattori, Yujiro</creatorcontrib><creatorcontrib>Ishii, Hirotaka</creatorcontrib><creatorcontrib>Tahara, Shigeyuki</creatorcontrib><creatorcontrib>Morita, Akio</creatorcontrib><creatorcontrib>Ozawa, Hitoshi</creatorcontrib><title>Accurate assessment of estrogen receptor profiles in non-functioning pituitary adenomas using RT-digital PCR and immunohistochemistry</title><title>Life sciences (1973)</title><description>Non-functioning pituitary adenomas (NFPAs) are common pituitary tumors, and surgery is generally the only treatment option. Few attempts have been made to explore target molecules for the development of NFPA pharmacological treatments.
We quantitatively assessed the expression profiles of estrogen receptor (ER) transcripts and proteins in NFPA samples, using reverse transcription-digital polymerase chain reaction (RT-dPCR) and immunohistochemistry, and further investigated the correlations between the expression levels of ER and those of downstream responsive genes. All patients had undergone surgery at the same high-volume hospital. A total of 20 patients with NFPAs were included. All patients were new-onset, and none were diagnosed with intratumoral hemorrhages or cysts.
NFPA samples exhibited a bimodal ESR1 expression pattern and were categorized into significantly different high- and low-ESR1 expression level groups (P < 0.05). In contrast, expression levels of ESR1 variants and ESR2 could barely be detected. Similar results were obtained through the immunohistochemical staining of NFPAs, using well-validated antibodies against ERs. The expression levels of ESR1 positively correlated with those of GREB1, an estrogen-responsive gene [correlation coefficient (r) = 0.623, P = 0.003].
ESR1 expression levels in NFPAs exhibited a bimodal pattern and were positively correlated with GREB1 expression levels. The accurate assessment of ER expression levels may further advance future NFPA-related research.
•Non-functioning pituitary adenomas are currently only treatable through surgery.•We examined estrogen receptor gene expression profiles in NFPAs using RT-dPCR.•We examined the ER protein expression using well-validated antibodies.•NFPA samples exhibited a bimodal ESR1 expression pattern.•ESR1 expression levels in NFPAs were positively correlated with GREB1 expression.</description><subject>Antibodies</subject><subject>Brain tumors</subject><subject>Correlation coefficient</subject><subject>Correlation coefficients</subject><subject>Cysts</subject><subject>Drug therapy</subject><subject>ESR1</subject><subject>ESR2</subject><subject>Estrogen receptors</subject><subject>Estrogen response gene</subject><subject>Estrogens</subject><subject>genes</subject><subject>GREB1</subject><subject>Hemorrhage</subject><subject>hospitals</subject><subject>Immunohistochemistry</subject><subject>Non-functioning pituitary adenomas</subject><subject>Patients</subject><subject>Pituitary</subject><subject>Polymerase chain reaction</subject><subject>Receptors</subject><subject>Reverse transcription</subject><subject>RT-dPCR</subject><subject>Surgery</subject><subject>Tumors</subject><issn>0024-3205</issn><issn>1879-0631</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNqFkc-KFDEQxoO44LjuA3gLePHSY_53Gk_L4KqwoCy75xCTymyG7mRM0sI-wL63GcaTBz0VRf2-Kr76EHpLyZYSqj4ctnOoW0ZY76kWVL1AG6rHaSCK05doQwgTA2dEvkKvaz0QQqQc-QY9Xzu3FtsA21qh1gVSwzlgqK3kPSRcwMGx5YKPJYc4Q8Ux4ZTTENbkWswppj0-xrbGZssTth5SXmzFaz0N7u4HH_d9NOPvuztsk8dxWdaUH2Nt2T3C0mt5eoMugp0rXP2pl-jh5tP97stw--3z19317eCEHNuguGAyAA-aaj6pbk_5UU4arB6dt8F5-MEmwZ1nYRrDpAJorxRRyks6Scov0fvz3m7m59o9mn7fwTzbBHmthklBhJ4Yn_6PCsG0UFLzjr77Cz3ktaRupFNKjpQIKjtFz5QrudYCwRxLXPrPDCXmlKE5mJ6hOWVozhl2zcezBvpTfkUoproIyYGPPZdmfI7_UP8GGlymDA</recordid><startdate>20201101</startdate><enddate>20201101</enddate><creator>Hattori, Yujiro</creator><creator>Ishii, Hirotaka</creator><creator>Tahara, Shigeyuki</creator><creator>Morita, Akio</creator><creator>Ozawa, Hitoshi</creator><general>Elsevier Inc</general><general>Elsevier BV</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7U7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope></search><sort><creationdate>20201101</creationdate><title>Accurate assessment of estrogen receptor profiles in non-functioning pituitary adenomas using RT-digital PCR and immunohistochemistry</title><author>Hattori, Yujiro ; Ishii, Hirotaka ; Tahara, Shigeyuki ; Morita, Akio ; Ozawa, Hitoshi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c457t-63425fe3f8183968796d7598ea87cdafcdeb2943cd2f97f96fe8d66066d519513</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Antibodies</topic><topic>Brain tumors</topic><topic>Correlation coefficient</topic><topic>Correlation coefficients</topic><topic>Cysts</topic><topic>Drug therapy</topic><topic>ESR1</topic><topic>ESR2</topic><topic>Estrogen receptors</topic><topic>Estrogen response gene</topic><topic>Estrogens</topic><topic>genes</topic><topic>GREB1</topic><topic>Hemorrhage</topic><topic>hospitals</topic><topic>Immunohistochemistry</topic><topic>Non-functioning pituitary adenomas</topic><topic>Patients</topic><topic>Pituitary</topic><topic>Polymerase chain reaction</topic><topic>Receptors</topic><topic>Reverse transcription</topic><topic>RT-dPCR</topic><topic>Surgery</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hattori, Yujiro</creatorcontrib><creatorcontrib>Ishii, Hirotaka</creatorcontrib><creatorcontrib>Tahara, Shigeyuki</creatorcontrib><creatorcontrib>Morita, Akio</creatorcontrib><creatorcontrib>Ozawa, Hitoshi</creatorcontrib><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>Life sciences (1973)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hattori, Yujiro</au><au>Ishii, Hirotaka</au><au>Tahara, Shigeyuki</au><au>Morita, Akio</au><au>Ozawa, Hitoshi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Accurate assessment of estrogen receptor profiles in non-functioning pituitary adenomas using RT-digital PCR and immunohistochemistry</atitle><jtitle>Life sciences (1973)</jtitle><date>2020-11-01</date><risdate>2020</risdate><volume>260</volume><spage>118416</spage><epage>7</epage><pages>118416-7</pages><artnum>118416</artnum><issn>0024-3205</issn><eissn>1879-0631</eissn><abstract>Non-functioning pituitary adenomas (NFPAs) are common pituitary tumors, and surgery is generally the only treatment option. Few attempts have been made to explore target molecules for the development of NFPA pharmacological treatments.
We quantitatively assessed the expression profiles of estrogen receptor (ER) transcripts and proteins in NFPA samples, using reverse transcription-digital polymerase chain reaction (RT-dPCR) and immunohistochemistry, and further investigated the correlations between the expression levels of ER and those of downstream responsive genes. All patients had undergone surgery at the same high-volume hospital. A total of 20 patients with NFPAs were included. All patients were new-onset, and none were diagnosed with intratumoral hemorrhages or cysts.
NFPA samples exhibited a bimodal ESR1 expression pattern and were categorized into significantly different high- and low-ESR1 expression level groups (P < 0.05). In contrast, expression levels of ESR1 variants and ESR2 could barely be detected. Similar results were obtained through the immunohistochemical staining of NFPAs, using well-validated antibodies against ERs. The expression levels of ESR1 positively correlated with those of GREB1, an estrogen-responsive gene [correlation coefficient (r) = 0.623, P = 0.003].
ESR1 expression levels in NFPAs exhibited a bimodal pattern and were positively correlated with GREB1 expression levels. The accurate assessment of ER expression levels may further advance future NFPA-related research.
•Non-functioning pituitary adenomas are currently only treatable through surgery.•We examined estrogen receptor gene expression profiles in NFPAs using RT-dPCR.•We examined the ER protein expression using well-validated antibodies.•NFPA samples exhibited a bimodal ESR1 expression pattern.•ESR1 expression levels in NFPAs were positively correlated with GREB1 expression.</abstract><cop>New York</cop><pub>Elsevier Inc</pub><doi>10.1016/j.lfs.2020.118416</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0024-3205 |
| ispartof | Life sciences (1973), 2020-11, Vol.260, p.118416-7, Article 118416 |
| issn | 0024-3205 1879-0631 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2540489239 |
| source | Elsevier ScienceDirect Journals |
| subjects | Antibodies Brain tumors Correlation coefficient Correlation coefficients Cysts Drug therapy ESR1 ESR2 Estrogen receptors Estrogen response gene Estrogens genes GREB1 Hemorrhage hospitals Immunohistochemistry Non-functioning pituitary adenomas Patients Pituitary Polymerase chain reaction Receptors Reverse transcription RT-dPCR Surgery Tumors |
| title | Accurate assessment of estrogen receptor profiles in non-functioning pituitary adenomas using RT-digital PCR and immunohistochemistry |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-05T04%3A09%3A02IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Accurate%20assessment%20of%20estrogen%20receptor%20profiles%20in%20non-functioning%20pituitary%20adenomas%20using%20RT-digital%20PCR%20and%20immunohistochemistry&rft.jtitle=Life%20sciences%20(1973)&rft.au=Hattori,%20Yujiro&rft.date=2020-11-01&rft.volume=260&rft.spage=118416&rft.epage=7&rft.pages=118416-7&rft.artnum=118416&rft.issn=0024-3205&rft.eissn=1879-0631&rft_id=info:doi/10.1016/j.lfs.2020.118416&rft_dat=%3Cproquest_cross%3E2442846583%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2465710415&rft_id=info:pmid/&rft_els_id=S0024320520311693&rfr_iscdi=true |