Global profiling of RNA-binding protein target sites by LACE-seq

RNA-binding proteins (RBPs) have essential functions during germline and early embryo development. However, current methods are unable to identify the in vivo targets of a RBP in these low-abundance cells. Here, by coupling RBP-mediated reverse transcription termination with linear amplification of...

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Veröffentlicht in:	Nature cell biology 2021-06, Vol.23 (6), p.664-675
Hauptverfasser:	Su, Ruibao, Fan, Li-Hua, Cao, Changchang, Wang, Lei, Du, Zongchang, Cai, Zhaokui, Ouyang, Ying-Chun, Wang, Yue, Zhou, Qian, Wu, Ligang, Zhang, Nan, Zhu, Xiaoxiao, Lei, Wen-Long, Zhao, Hailian, Tian, Yong, He, Shunmin, Wong, Catherine C. L., Sun, Qing-Yuan, Xue, Yuanchao
Format:	Artikel
Sprache:	eng
Schlagworte:	631/136/2434/1706 631/1647/514/2254 631/337/384/521 631/337/574 Animals Argonaute 2 protein Argonaute Proteins - genetics Argonaute Proteins - metabolism Binding Sites Biomedical and Life Sciences Cancer Research Cell Biology Complementary DNA DEAD-box RNA Helicases - genetics DEAD-box RNA Helicases - metabolism Deoxyribonucleic acid Developmental Biology DNA DNA sequencing Embryogenesis Embryonic growth stage Embryos Female Gametocytes Gene expression Gene Expression Profiling Gene Expression Regulation, Developmental HeLa Cells Heterogeneous-Nuclear Ribonucleoproteins - genetics Heterogeneous-Nuclear Ribonucleoproteins - metabolism High-Throughput Nucleotide Sequencing Humans In vivo methods and tests K562 Cells Lace Life Sciences Long terminal repeat Mice Mice, Inbred C57BL Mice, Inbred ICR Mice, Knockout Nucleotides Oocytes Oocytes - metabolism Polypyrimidine Tract-Binding Protein - genetics Polypyrimidine Tract-Binding Protein - metabolism Protein Binding Proteins Proteomics Regulatory mechanisms (biology) Reverse transcription Ribonucleic acid RNA RNA, Messenger - genetics RNA, Messenger - metabolism RNA, Small Interfering - genetics RNA, Small Interfering - metabolism RNA-binding protein RNA-Binding Proteins - genetics RNA-Binding Proteins - metabolism RNA-Seq siRNA Slicing Stem Cells Target recognition technical-report Transcription termination Transcriptome Transcriptomes Transcriptomics
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