The relation between ACKR4 and CCR7 genes expression and breast cancer metastasis
Breast cancer is the most severe malignant tumor in women. Chemokines and their receptors appear to be implicated in tumorigenesis and metastatic pattern. Also the scavenger atypical chemokine receptors are emerging as crucial regulators for the availability of chemokines. Therefore the aim of the p...
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| Veröffentlicht in: | Life sciences (1973) 2021-08, Vol.279, p.119691-119691, Article 119691 |
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| container_title | Life sciences (1973) |
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| creator | Mohammed, Mostafa M. Shaker, Olfat Ramzy, Maggie M. Gaber, Shereen S. Kamel, Heba S. Abed EL Baky, Mohamed F. |
| description | Breast cancer is the most severe malignant tumor in women. Chemokines and their receptors appear to be implicated in tumorigenesis and metastatic pattern. Also the scavenger atypical chemokine receptors are emerging as crucial regulators for the availability of chemokines. Therefore the aim of the present study is to evaluate the expression of CCR7, ACKR4 and their ligand; CCL21 in human breast cancer.
In this study, RT-PCR was done to detect the expression of CCR7 and ACKR4 in 50 non-metastatic and 30 metastatic breast cancer tissue. Also CCL21 level in the serum of study group was detected by ELISA. The expression of all markers is compared to 80 control healthy individual.
Our results revealed the increase in expression of CCR7 and CCL21 level in metastatic group compared to non-metastatic and control groups while ACKR4 expression is significantly increased in breast tissues of non-metastatic patients compared to both control and metastatic groups. Also there was significant positive correlation between CCR7 expression and CCL21 level in cancer patients and significant negative correlation between ACKR4 and both CCR-7 and CCL21 in both non-metastatic and metastatic cancer groups.
Thus, it might be elucidating that ACKR4 and CCR7 could be a novel target for tumor therapy as targeting the chemokine-receptors axis might represent a powerful tool to prevent infiltration and metastasis and consequently improve cancer prognosis and treatment. |
| doi_str_mv | 10.1016/j.lfs.2021.119691 |
| format | Article |
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In this study, RT-PCR was done to detect the expression of CCR7 and ACKR4 in 50 non-metastatic and 30 metastatic breast cancer tissue. Also CCL21 level in the serum of study group was detected by ELISA. The expression of all markers is compared to 80 control healthy individual.
Our results revealed the increase in expression of CCR7 and CCL21 level in metastatic group compared to non-metastatic and control groups while ACKR4 expression is significantly increased in breast tissues of non-metastatic patients compared to both control and metastatic groups. Also there was significant positive correlation between CCR7 expression and CCL21 level in cancer patients and significant negative correlation between ACKR4 and both CCR-7 and CCL21 in both non-metastatic and metastatic cancer groups.
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In this study, RT-PCR was done to detect the expression of CCR7 and ACKR4 in 50 non-metastatic and 30 metastatic breast cancer tissue. Also CCL21 level in the serum of study group was detected by ELISA. The expression of all markers is compared to 80 control healthy individual.
Our results revealed the increase in expression of CCR7 and CCL21 level in metastatic group compared to non-metastatic and control groups while ACKR4 expression is significantly increased in breast tissues of non-metastatic patients compared to both control and metastatic groups. Also there was significant positive correlation between CCR7 expression and CCL21 level in cancer patients and significant negative correlation between ACKR4 and both CCR-7 and CCL21 in both non-metastatic and metastatic cancer groups.
Thus, it might be elucidating that ACKR4 and CCR7 could be a novel target for tumor therapy as targeting the chemokine-receptors axis might represent a powerful tool to prevent infiltration and metastasis and consequently improve cancer prognosis and treatment.</description><subject>ACKR4</subject><subject>Atypical chemokine receptors</subject><subject>Breast cancer</subject><subject>CC chemokine receptors</subject><subject>CCL21</subject><subject>CCL21 protein</subject><subject>CCR7</subject><subject>CCR7 protein</subject><subject>Chemokine receptors</subject><subject>Chemokines</subject><subject>Gene expression</subject><subject>Medical prognosis</subject><subject>Metastases</subject><subject>Metastasis</subject><subject>Patients</subject><subject>Polymerase chain reaction</subject><subject>Receptors</subject><subject>Tumorigenesis</subject><subject>Tumors</subject><issn>0024-3205</issn><issn>1879-0631</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNp9kE1LxDAQhoMouK7-AG8BL15aM0nTNnhail-4IC7rOaTpVLt02zXp-vHvTaknD56GYZ53mHkIOQcWA4P0ahO3tY854xADqFTBAZlBnqmIpQIOyYwxnkSCM3lMTrzfMMakzMSMPK_fkDpszdD0HS1x-ETs6KJ4XCXUdBUtilVGX7FDT_Fr59D7kRsnpUPjB2pNZ9HRLQ6hM77xp-SoNq3Hs986Jy-3N-viPlo-3T0Ui2VkhcyHSFVJjYgyzcO9iawYr4XJclUpKLPUZpUQkNnwSMk5S3mdoqqrMAHFWQ4KxJxcTnt3rn_fox_0tvEW29Z02O-95lIoyYEzEdCLP-im37suXBcoCRlACixQMFHW9d47rPXONVvjvjUwPUrWGx0k61GyniSHzPWUwfDpR4NOe9tgMFI1Du2gq775J_0DgJWBpg</recordid><startdate>20210815</startdate><enddate>20210815</enddate><creator>Mohammed, Mostafa M.</creator><creator>Shaker, Olfat</creator><creator>Ramzy, Maggie M.</creator><creator>Gaber, Shereen S.</creator><creator>Kamel, Heba S.</creator><creator>Abed EL Baky, Mohamed F.</creator><general>Elsevier Inc</general><general>Elsevier BV</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7U7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20210815</creationdate><title>The relation between ACKR4 and CCR7 genes expression and breast cancer metastasis</title><author>Mohammed, Mostafa M. ; Shaker, Olfat ; Ramzy, Maggie M. ; Gaber, Shereen S. ; Kamel, Heba S. ; Abed EL Baky, Mohamed F.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c358t-9d4feee56811945d02f3a789d91b76c7d3317c691b22062f6e9fdb76192081913</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>ACKR4</topic><topic>Atypical chemokine receptors</topic><topic>Breast cancer</topic><topic>CC chemokine receptors</topic><topic>CCL21</topic><topic>CCL21 protein</topic><topic>CCR7</topic><topic>CCR7 protein</topic><topic>Chemokine receptors</topic><topic>Chemokines</topic><topic>Gene expression</topic><topic>Medical prognosis</topic><topic>Metastases</topic><topic>Metastasis</topic><topic>Patients</topic><topic>Polymerase chain reaction</topic><topic>Receptors</topic><topic>Tumorigenesis</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mohammed, Mostafa M.</creatorcontrib><creatorcontrib>Shaker, Olfat</creatorcontrib><creatorcontrib>Ramzy, Maggie M.</creatorcontrib><creatorcontrib>Gaber, Shereen S.</creatorcontrib><creatorcontrib>Kamel, Heba S.</creatorcontrib><creatorcontrib>Abed EL Baky, Mohamed F.</creatorcontrib><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Life sciences (1973)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mohammed, Mostafa M.</au><au>Shaker, Olfat</au><au>Ramzy, Maggie M.</au><au>Gaber, Shereen S.</au><au>Kamel, Heba S.</au><au>Abed EL Baky, Mohamed F.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The relation between ACKR4 and CCR7 genes expression and breast cancer metastasis</atitle><jtitle>Life sciences (1973)</jtitle><date>2021-08-15</date><risdate>2021</risdate><volume>279</volume><spage>119691</spage><epage>119691</epage><pages>119691-119691</pages><artnum>119691</artnum><issn>0024-3205</issn><eissn>1879-0631</eissn><abstract>Breast cancer is the most severe malignant tumor in women. Chemokines and their receptors appear to be implicated in tumorigenesis and metastatic pattern. Also the scavenger atypical chemokine receptors are emerging as crucial regulators for the availability of chemokines. Therefore the aim of the present study is to evaluate the expression of CCR7, ACKR4 and their ligand; CCL21 in human breast cancer.
In this study, RT-PCR was done to detect the expression of CCR7 and ACKR4 in 50 non-metastatic and 30 metastatic breast cancer tissue. Also CCL21 level in the serum of study group was detected by ELISA. The expression of all markers is compared to 80 control healthy individual.
Our results revealed the increase in expression of CCR7 and CCL21 level in metastatic group compared to non-metastatic and control groups while ACKR4 expression is significantly increased in breast tissues of non-metastatic patients compared to both control and metastatic groups. Also there was significant positive correlation between CCR7 expression and CCL21 level in cancer patients and significant negative correlation between ACKR4 and both CCR-7 and CCL21 in both non-metastatic and metastatic cancer groups.
Thus, it might be elucidating that ACKR4 and CCR7 could be a novel target for tumor therapy as targeting the chemokine-receptors axis might represent a powerful tool to prevent infiltration and metastasis and consequently improve cancer prognosis and treatment.</abstract><cop>New York</cop><pub>Elsevier Inc</pub><doi>10.1016/j.lfs.2021.119691</doi><tpages>1</tpages></addata></record> |
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| subjects | ACKR4 Atypical chemokine receptors Breast cancer CC chemokine receptors CCL21 CCL21 protein CCR7 CCR7 protein Chemokine receptors Chemokines Gene expression Medical prognosis Metastases Metastasis Patients Polymerase chain reaction Receptors Tumorigenesis Tumors |
| title | The relation between ACKR4 and CCR7 genes expression and breast cancer metastasis |
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