Self-assembling peptide hydrogel SPG-178 as a pancreatic fistula-preventing agent
Purpose Pancreatic fistula (PF) is a common and challenging complication after pancreatic surgery. The aim of this study was to investigate the efficacy of a new method for preventing PF utilizing self-assembling peptide hydrogel SPG-178 as a preclinical study. Methods The degradability of SPG-178 w...
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| Veröffentlicht in: | Langenbeck's archives of surgery 2022-02, Vol.407 (1), p.189-196 |
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| creator | Mikamori, Manabu Gotoh, Kunihito Kobayashi, Shogo Uesugi, Koji Iwagami, Yoshifumi Yamada, Daisaku Tomimaru, Yoshito Akita, Hirofumi Noda, Takehiro Doki, Yuichiro Eguchi, Hidetoshi |
| description | Purpose
Pancreatic fistula (PF) is a common and challenging complication after pancreatic surgery. The aim of this study was to investigate the efficacy of a new method for preventing PF utilizing self-assembling peptide hydrogel SPG-178 as a preclinical study.
Methods
The degradability of SPG-178 was confirmed by mixing it with protease. A PF rat model was then established to investigate the efficacy of SPG-178 at preventing PF. After transecting the pancreatic duct toward the spleen, SPG-178 was attached to both sides of the pancreatic stump. The levels of amylase and lipase in both the serum and ascites were measured and surgical specimens investigated pathologically.
Results
The hardness of SPG-178 did not change when treated with protease over a short period. The ascitic amylase level was significantly lower in rats treated with SPG-178 than rats who were not 3 days after transection of the pancreatic duct toward the spleen. Pathological examination showed fewer inflammatory cells and presence of a structure body on the surface of the pancreatic stump in the SPG-178-treated group. SPG-178 remained on the surface and many cells that covered it formed fibrous tissue or mesothelium.
Conclusion
Self-assembling peptide hydrogel SPG-178 has potential as a tool for preventing PF. |
| doi_str_mv | 10.1007/s00423-021-02226-5 |
| format | Article |
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Pancreatic fistula (PF) is a common and challenging complication after pancreatic surgery. The aim of this study was to investigate the efficacy of a new method for preventing PF utilizing self-assembling peptide hydrogel SPG-178 as a preclinical study.
Methods
The degradability of SPG-178 was confirmed by mixing it with protease. A PF rat model was then established to investigate the efficacy of SPG-178 at preventing PF. After transecting the pancreatic duct toward the spleen, SPG-178 was attached to both sides of the pancreatic stump. The levels of amylase and lipase in both the serum and ascites were measured and surgical specimens investigated pathologically.
Results
The hardness of SPG-178 did not change when treated with protease over a short period. The ascitic amylase level was significantly lower in rats treated with SPG-178 than rats who were not 3 days after transection of the pancreatic duct toward the spleen. Pathological examination showed fewer inflammatory cells and presence of a structure body on the surface of the pancreatic stump in the SPG-178-treated group. SPG-178 remained on the surface and many cells that covered it formed fibrous tissue or mesothelium.
Conclusion
Self-assembling peptide hydrogel SPG-178 has potential as a tool for preventing PF.</description><identifier>ISSN: 1435-2443</identifier><identifier>EISSN: 1435-2451</identifier><identifier>DOI: 10.1007/s00423-021-02226-5</identifier><identifier>PMID: 34100123</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Abdominal Surgery ; Amylases ; Animals ; Cardiac Surgery ; General Surgery ; Hydrogels ; Medicine ; Medicine & Public Health ; Original Article ; Pancreatic Fistula - prevention & control ; Pancreaticoduodenectomy ; Peptides ; Rats ; Thoracic Surgery ; Traumatic Surgery ; Vascular Surgery</subject><ispartof>Langenbeck's archives of surgery, 2022-02, Vol.407 (1), p.189-196</ispartof><rights>The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2021</rights><rights>2021. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c347t-4f836a71dbbd09bcb4588e244de349c1acc46e929d465a8b1148b5d93e137f263</citedby><cites>FETCH-LOGICAL-c347t-4f836a71dbbd09bcb4588e244de349c1acc46e929d465a8b1148b5d93e137f263</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00423-021-02226-5$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00423-021-02226-5$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,778,782,27911,27912,41475,42544,51306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34100123$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mikamori, Manabu</creatorcontrib><creatorcontrib>Gotoh, Kunihito</creatorcontrib><creatorcontrib>Kobayashi, Shogo</creatorcontrib><creatorcontrib>Uesugi, Koji</creatorcontrib><creatorcontrib>Iwagami, Yoshifumi</creatorcontrib><creatorcontrib>Yamada, Daisaku</creatorcontrib><creatorcontrib>Tomimaru, Yoshito</creatorcontrib><creatorcontrib>Akita, Hirofumi</creatorcontrib><creatorcontrib>Noda, Takehiro</creatorcontrib><creatorcontrib>Doki, Yuichiro</creatorcontrib><creatorcontrib>Eguchi, Hidetoshi</creatorcontrib><title>Self-assembling peptide hydrogel SPG-178 as a pancreatic fistula-preventing agent</title><title>Langenbeck's archives of surgery</title><addtitle>Langenbecks Arch Surg</addtitle><addtitle>Langenbecks Arch Surg</addtitle><description>Purpose
Pancreatic fistula (PF) is a common and challenging complication after pancreatic surgery. The aim of this study was to investigate the efficacy of a new method for preventing PF utilizing self-assembling peptide hydrogel SPG-178 as a preclinical study.
Methods
The degradability of SPG-178 was confirmed by mixing it with protease. A PF rat model was then established to investigate the efficacy of SPG-178 at preventing PF. After transecting the pancreatic duct toward the spleen, SPG-178 was attached to both sides of the pancreatic stump. The levels of amylase and lipase in both the serum and ascites were measured and surgical specimens investigated pathologically.
Results
The hardness of SPG-178 did not change when treated with protease over a short period. The ascitic amylase level was significantly lower in rats treated with SPG-178 than rats who were not 3 days after transection of the pancreatic duct toward the spleen. Pathological examination showed fewer inflammatory cells and presence of a structure body on the surface of the pancreatic stump in the SPG-178-treated group. SPG-178 remained on the surface and many cells that covered it formed fibrous tissue or mesothelium.
Conclusion
Self-assembling peptide hydrogel SPG-178 has potential as a tool for preventing PF.</description><subject>Abdominal Surgery</subject><subject>Amylases</subject><subject>Animals</subject><subject>Cardiac Surgery</subject><subject>General Surgery</subject><subject>Hydrogels</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Original Article</subject><subject>Pancreatic Fistula - prevention & control</subject><subject>Pancreaticoduodenectomy</subject><subject>Peptides</subject><subject>Rats</subject><subject>Thoracic Surgery</subject><subject>Traumatic Surgery</subject><subject>Vascular Surgery</subject><issn>1435-2443</issn><issn>1435-2451</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMtOwzAQRS0EolD4ARYoSzYGP_NYogoKUiVAhbVlO5OQKi_sBKl_j0tKlyxGM9LcezVzELqi5JYSktx5QgTjmDAairEYyyN0RgWXmAlJjw-z4DN07v2GEBInmThFMy5CAGX8DL2toS6w9h4aU1dtGfXQD1UO0ec2d10JdbR-XWKapJH2kY563VoHeqhsVFR-GGuNewff0A47ry7DcIFOCl17uNz3Ofp4fHhfPOHVy_J5cb_ClotkwKJIeawTmhuTk8xYI2SaQjg2By4yS7W1IoaMZbmIpU4NpSI1Ms84UJ4ULOZzdDPl9q77GsEPqqm8hbrWLXSjV0zyjBHJMhGkbJJa13nvoFC9qxrttooStUOpJpQqoFS_KJUMput9_mgayA-WP3ZBwCeBD6u2BKc23eja8PN_sT93sH3P</recordid><startdate>20220201</startdate><enddate>20220201</enddate><creator>Mikamori, Manabu</creator><creator>Gotoh, Kunihito</creator><creator>Kobayashi, Shogo</creator><creator>Uesugi, Koji</creator><creator>Iwagami, Yoshifumi</creator><creator>Yamada, Daisaku</creator><creator>Tomimaru, Yoshito</creator><creator>Akita, Hirofumi</creator><creator>Noda, Takehiro</creator><creator>Doki, Yuichiro</creator><creator>Eguchi, Hidetoshi</creator><general>Springer Berlin Heidelberg</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20220201</creationdate><title>Self-assembling peptide hydrogel SPG-178 as a pancreatic fistula-preventing agent</title><author>Mikamori, Manabu ; Gotoh, Kunihito ; Kobayashi, Shogo ; Uesugi, Koji ; Iwagami, Yoshifumi ; Yamada, Daisaku ; Tomimaru, Yoshito ; Akita, Hirofumi ; Noda, Takehiro ; Doki, Yuichiro ; Eguchi, Hidetoshi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c347t-4f836a71dbbd09bcb4588e244de349c1acc46e929d465a8b1148b5d93e137f263</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Abdominal Surgery</topic><topic>Amylases</topic><topic>Animals</topic><topic>Cardiac Surgery</topic><topic>General Surgery</topic><topic>Hydrogels</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Original Article</topic><topic>Pancreatic Fistula - prevention & control</topic><topic>Pancreaticoduodenectomy</topic><topic>Peptides</topic><topic>Rats</topic><topic>Thoracic Surgery</topic><topic>Traumatic Surgery</topic><topic>Vascular Surgery</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mikamori, Manabu</creatorcontrib><creatorcontrib>Gotoh, Kunihito</creatorcontrib><creatorcontrib>Kobayashi, Shogo</creatorcontrib><creatorcontrib>Uesugi, Koji</creatorcontrib><creatorcontrib>Iwagami, Yoshifumi</creatorcontrib><creatorcontrib>Yamada, Daisaku</creatorcontrib><creatorcontrib>Tomimaru, Yoshito</creatorcontrib><creatorcontrib>Akita, Hirofumi</creatorcontrib><creatorcontrib>Noda, Takehiro</creatorcontrib><creatorcontrib>Doki, Yuichiro</creatorcontrib><creatorcontrib>Eguchi, Hidetoshi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Langenbeck's archives of surgery</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mikamori, Manabu</au><au>Gotoh, Kunihito</au><au>Kobayashi, Shogo</au><au>Uesugi, Koji</au><au>Iwagami, Yoshifumi</au><au>Yamada, Daisaku</au><au>Tomimaru, Yoshito</au><au>Akita, Hirofumi</au><au>Noda, Takehiro</au><au>Doki, Yuichiro</au><au>Eguchi, Hidetoshi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Self-assembling peptide hydrogel SPG-178 as a pancreatic fistula-preventing agent</atitle><jtitle>Langenbeck's archives of surgery</jtitle><stitle>Langenbecks Arch Surg</stitle><addtitle>Langenbecks Arch Surg</addtitle><date>2022-02-01</date><risdate>2022</risdate><volume>407</volume><issue>1</issue><spage>189</spage><epage>196</epage><pages>189-196</pages><issn>1435-2443</issn><eissn>1435-2451</eissn><abstract>Purpose
Pancreatic fistula (PF) is a common and challenging complication after pancreatic surgery. The aim of this study was to investigate the efficacy of a new method for preventing PF utilizing self-assembling peptide hydrogel SPG-178 as a preclinical study.
Methods
The degradability of SPG-178 was confirmed by mixing it with protease. A PF rat model was then established to investigate the efficacy of SPG-178 at preventing PF. After transecting the pancreatic duct toward the spleen, SPG-178 was attached to both sides of the pancreatic stump. The levels of amylase and lipase in both the serum and ascites were measured and surgical specimens investigated pathologically.
Results
The hardness of SPG-178 did not change when treated with protease over a short period. The ascitic amylase level was significantly lower in rats treated with SPG-178 than rats who were not 3 days after transection of the pancreatic duct toward the spleen. Pathological examination showed fewer inflammatory cells and presence of a structure body on the surface of the pancreatic stump in the SPG-178-treated group. SPG-178 remained on the surface and many cells that covered it formed fibrous tissue or mesothelium.
Conclusion
Self-assembling peptide hydrogel SPG-178 has potential as a tool for preventing PF.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>34100123</pmid><doi>10.1007/s00423-021-02226-5</doi><tpages>8</tpages></addata></record> |
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| source | MEDLINE; Springer Nature - Complete Springer Journals |
| subjects | Abdominal Surgery Amylases Animals Cardiac Surgery General Surgery Hydrogels Medicine Medicine & Public Health Original Article Pancreatic Fistula - prevention & control Pancreaticoduodenectomy Peptides Rats Thoracic Surgery Traumatic Surgery Vascular Surgery |
| title | Self-assembling peptide hydrogel SPG-178 as a pancreatic fistula-preventing agent |
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