Cytotoxicity and genotoxicity of salicylate‐ and calcium silicate‐based root canal sealers on primer human periodontal ligament fibroblasts
The aim of this study was to evaluate the biocompatibility of epoxy‐resin‐based AHPlus, salicylate‐based MTA‐Fillapex and calcium silicate‐based iRootSP root canal sealers. Cytotoxicity was assessed by XTT test. The extracts from sealers of different setting times were serially diluted. Cell viabili...
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| Veröffentlicht in: | Australian endodontic journal 2021-12, Vol.47 (3), p.645-653 |
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| creator | Erdogan, Hilal Yildirim, Sibel Cobankara, Funda Kont |
| description | The aim of this study was to evaluate the biocompatibility of epoxy‐resin‐based AHPlus, salicylate‐based MTA‐Fillapex and calcium silicate‐based iRootSP root canal sealers. Cytotoxicity was assessed by XTT test. The extracts from sealers of different setting times were serially diluted. Cell viability was calculated as the percentage of the control group (100%). The optimal concentration of each sealer was used at genotoxicity test, and micronuclei formations were detected. Statistical analyses were done by using Kruskal–Wallis and Dunn post hoc test with Bonferroni correction. AHPlus and MTA‐Fillapex showed the lowest percentage of cell viability at higher concentrations (1:1, 1:2, 1:4), especially at first 12 h. iRootSP showed higher viability at all concentrations and times than AHPlus and MTA‐Fillapex. At genotoxicity assay, AHPlus increased the number of micronuclei. MTA‐Fillapex slightly induced micronucleus formation (not significant) and iRootSP was not increased. In conclusion, calcium silicate‐based iRootSP had lowest cytotoxic and genotoxic potential and can be considered as a highly biocompatible material. |
| doi_str_mv | 10.1111/aej.12537 |
| format | Article |
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Cytotoxicity was assessed by XTT test. The extracts from sealers of different setting times were serially diluted. Cell viability was calculated as the percentage of the control group (100%). The optimal concentration of each sealer was used at genotoxicity test, and micronuclei formations were detected. Statistical analyses were done by using Kruskal–Wallis and Dunn post hoc test with Bonferroni correction. AHPlus and MTA‐Fillapex showed the lowest percentage of cell viability at higher concentrations (1:1, 1:2, 1:4), especially at first 12 h. iRootSP showed higher viability at all concentrations and times than AHPlus and MTA‐Fillapex. At genotoxicity assay, AHPlus increased the number of micronuclei. MTA‐Fillapex slightly induced micronucleus formation (not significant) and iRootSP was not increased. In conclusion, calcium silicate‐based iRootSP had lowest cytotoxic and genotoxic potential and can be considered as a highly biocompatible material.</description><identifier>ISSN: 1329-1947</identifier><identifier>EISSN: 1747-4477</identifier><identifier>DOI: 10.1111/aej.12537</identifier><identifier>PMID: 34097343</identifier><language>eng</language><publisher>Australia: Wiley Subscription Services, Inc</publisher><subject>bioactive ; Biocompatibility ; biomaterials ; Calcium ; Calcium Compounds ; calcium silicate ; Cell viability ; Cytotoxicity ; Dental Pulp Cavity ; DNA Damage ; Endodontics ; Fibroblasts ; Genotoxicity ; Humans ; Micronuclei ; Periodontal Ligament ; Root canals ; salicylate ; Salicylates - toxicity ; Salicylic acid ; Sealing compounds ; Silicates ; Statistical analysis</subject><ispartof>Australian endodontic journal, 2021-12, Vol.47 (3), p.645-653</ispartof><rights>2021 Australian Society of Endodontology Inc</rights><rights>2021 Australian Society of Endodontology Inc.</rights><rights>Copyright © 2021 Australian Society of Endodontology Inc</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3537-d4f42d6aa221c2989756fea0c6b5e9da20eb623c853ccf2acbb6fffcd722e0d83</citedby><cites>FETCH-LOGICAL-c3537-d4f42d6aa221c2989756fea0c6b5e9da20eb623c853ccf2acbb6fffcd722e0d83</cites><orcidid>0000-0001-8044-1874 ; 0000-0001-5219-4693 ; 0000-0003-3031-0790</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Faej.12537$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Faej.12537$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34097343$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Erdogan, Hilal</creatorcontrib><creatorcontrib>Yildirim, Sibel</creatorcontrib><creatorcontrib>Cobankara, Funda Kont</creatorcontrib><title>Cytotoxicity and genotoxicity of salicylate‐ and calcium silicate‐based root canal sealers on primer human periodontal ligament fibroblasts</title><title>Australian endodontic journal</title><addtitle>Aust Endod J</addtitle><description>The aim of this study was to evaluate the biocompatibility of epoxy‐resin‐based AHPlus, salicylate‐based MTA‐Fillapex and calcium silicate‐based iRootSP root canal sealers. 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In conclusion, calcium silicate‐based iRootSP had lowest cytotoxic and genotoxic potential and can be considered as a highly biocompatible material.</description><subject>bioactive</subject><subject>Biocompatibility</subject><subject>biomaterials</subject><subject>Calcium</subject><subject>Calcium Compounds</subject><subject>calcium silicate</subject><subject>Cell viability</subject><subject>Cytotoxicity</subject><subject>Dental Pulp Cavity</subject><subject>DNA Damage</subject><subject>Endodontics</subject><subject>Fibroblasts</subject><subject>Genotoxicity</subject><subject>Humans</subject><subject>Micronuclei</subject><subject>Periodontal Ligament</subject><subject>Root canals</subject><subject>salicylate</subject><subject>Salicylates - toxicity</subject><subject>Salicylic acid</subject><subject>Sealing compounds</subject><subject>Silicates</subject><subject>Statistical analysis</subject><issn>1329-1947</issn><issn>1747-4477</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kc1u1TAQhS0Eoj-w4AWQJTZ0kdaxnThZVlflT5XYwDqa2OPiKycutiPIjjeAZ-RJ8G0KSEjMxuOZT0c6cwh5VrPzutQF4P685o1QD8hxraSqpFTqYekF76u6l-qInKS0Z4xLoerH5EhI1ishxTH5vltzyOGr0y6vFGZDb3D-OwiWJvBOrx4y_vz2447Q4LVbJppc2WzzERIaGkPIZTuDpwnBY0w0zPQ2ugkj_bRMUD4YXTBhzoXx7gYmnDO1boxh9JByekIeWfAJn96_p-Tjq6sPuzfV9fvXb3eX15UWxWdlpJXctACc15r3Xa-a1iIw3Y4N9gY4w7HlQneN0Npy0OPYWmu1UZwjM504JS833dsYPi-Y8jC5pNF7mDEsaSjX7JjspOoL-uIfdB-WWEwWqi3nFw1vDoJnG6VjSCmiHQ6-Ia5DzYZDSkNJabhLqbDP7xWXcULzh_wdSwEuNuCL87j-X2m4vHq3Sf4CpkOhYw</recordid><startdate>202112</startdate><enddate>202112</enddate><creator>Erdogan, Hilal</creator><creator>Yildirim, Sibel</creator><creator>Cobankara, Funda Kont</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>K9.</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-8044-1874</orcidid><orcidid>https://orcid.org/0000-0001-5219-4693</orcidid><orcidid>https://orcid.org/0000-0003-3031-0790</orcidid></search><sort><creationdate>202112</creationdate><title>Cytotoxicity and genotoxicity of salicylate‐ and calcium silicate‐based root canal sealers on primer human periodontal ligament fibroblasts</title><author>Erdogan, Hilal ; Yildirim, Sibel ; Cobankara, Funda Kont</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3537-d4f42d6aa221c2989756fea0c6b5e9da20eb623c853ccf2acbb6fffcd722e0d83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>bioactive</topic><topic>Biocompatibility</topic><topic>biomaterials</topic><topic>Calcium</topic><topic>Calcium Compounds</topic><topic>calcium silicate</topic><topic>Cell viability</topic><topic>Cytotoxicity</topic><topic>Dental Pulp Cavity</topic><topic>DNA Damage</topic><topic>Endodontics</topic><topic>Fibroblasts</topic><topic>Genotoxicity</topic><topic>Humans</topic><topic>Micronuclei</topic><topic>Periodontal Ligament</topic><topic>Root canals</topic><topic>salicylate</topic><topic>Salicylates - toxicity</topic><topic>Salicylic acid</topic><topic>Sealing compounds</topic><topic>Silicates</topic><topic>Statistical analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Erdogan, Hilal</creatorcontrib><creatorcontrib>Yildirim, Sibel</creatorcontrib><creatorcontrib>Cobankara, Funda Kont</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>Australian endodontic journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Erdogan, Hilal</au><au>Yildirim, Sibel</au><au>Cobankara, Funda Kont</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cytotoxicity and genotoxicity of salicylate‐ and calcium silicate‐based root canal sealers on primer human periodontal ligament fibroblasts</atitle><jtitle>Australian endodontic journal</jtitle><addtitle>Aust Endod J</addtitle><date>2021-12</date><risdate>2021</risdate><volume>47</volume><issue>3</issue><spage>645</spage><epage>653</epage><pages>645-653</pages><issn>1329-1947</issn><eissn>1747-4477</eissn><abstract>The aim of this study was to evaluate the biocompatibility of epoxy‐resin‐based AHPlus, salicylate‐based MTA‐Fillapex and calcium silicate‐based iRootSP root canal sealers. 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| subjects | bioactive Biocompatibility biomaterials Calcium Calcium Compounds calcium silicate Cell viability Cytotoxicity Dental Pulp Cavity DNA Damage Endodontics Fibroblasts Genotoxicity Humans Micronuclei Periodontal Ligament Root canals salicylate Salicylates - toxicity Salicylic acid Sealing compounds Silicates Statistical analysis |
| title | Cytotoxicity and genotoxicity of salicylate‐ and calcium silicate‐based root canal sealers on primer human periodontal ligament fibroblasts |
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