Automation of high CHO cell density seed intensification via online control of the cell specific perfusion rate and its impact on the N-stage inoculum quality

•Improvement of conventional CHO seed train strategies.•Precise online N-1 perfusion rate control facilitates process intensification.•Importance of accurate CSPR control for generation of high quality inocula.•Metabolic switches and apoptotic response can result from false perfusion control. Curren...

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Veröffentlicht in:	Journal of biotechnology 2021-07, Vol.335, p.65-75
Hauptverfasser:	Schulze, Markus, Lemke, Johannes, Pollard, David, Wijffels, Rene H., Matuszczyk, Jens, Martens, Dirk E.
Format:	Artikel
Sprache:	eng
Schlagworte:	Capacitance Cell-specific perfusion rate (CSPR) CHO cell culture N-1 perfusion Online control Process intensification
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