The Transcription Factor Gene tclB2 Regulates Mannanolytic Enzyme Production in the Fungus Talaromyces cellulolyticus
The filamentous fungus Talaromyces cellulolyticus is a well-characterized cellulolytic and hemicellulolytic enzyme producer. In this study, the function of the tclB2 gene, which is a homolog of the manR / clrB / clr-2 gene in other filamentous fungi, in mannanolytic enzyme production by T. celluloly...
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| Veröffentlicht in: | Applied biochemistry and biotechnology 2021-10, Vol.193 (10), p.3163-3172 |
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| creator | Fujii, Tatsuya Inoue, Hiroyuki Matsushika, Akinori |
| description | The filamentous fungus
Talaromyces cellulolyticus
is a well-characterized cellulolytic and hemicellulolytic enzyme producer. In this study, the function of the
tclB2
gene, which is a homolog of the
manR
/
clrB
/
clr-2
gene in other filamentous fungi, in mannanolytic enzyme production by
T. cellulolyticus
was investigated. When a
tclB2
-disrupted strain (YDTclB) was grown in the presence of glucomannan, the production of β-mannanase, β-mannosidase, and α-galactosidase was decreased at the protein and transcriptional levels when compared to the control strain. In addition, a
tclB2
-overexpressing strain (YHTclB) showed higher β-mannanase and β-mannosidase production. When cellulose was used as a carbon source, the expression of genes encoding mannanolytic enzymes also decreased in YDTclB. These results suggested that TclB2 contributes to mannanolytic enzyme production in
T. cellulolyticus
. This work is the first study to identify a transcriptional regulator of mannanolytic enzyme genes in
T. cellulolyticus
. |
| doi_str_mv | 10.1007/s12010-021-03588-8 |
| format | Article |
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Talaromyces cellulolyticus
is a well-characterized cellulolytic and hemicellulolytic enzyme producer. In this study, the function of the
tclB2
gene, which is a homolog of the
manR
/
clrB
/
clr-2
gene in other filamentous fungi, in mannanolytic enzyme production by
T. cellulolyticus
was investigated. When a
tclB2
-disrupted strain (YDTclB) was grown in the presence of glucomannan, the production of β-mannanase, β-mannosidase, and α-galactosidase was decreased at the protein and transcriptional levels when compared to the control strain. In addition, a
tclB2
-overexpressing strain (YHTclB) showed higher β-mannanase and β-mannosidase production. When cellulose was used as a carbon source, the expression of genes encoding mannanolytic enzymes also decreased in YDTclB. These results suggested that TclB2 contributes to mannanolytic enzyme production in
T. cellulolyticus
. This work is the first study to identify a transcriptional regulator of mannanolytic enzyme genes in
T. cellulolyticus
.</description><identifier>ISSN: 0273-2289</identifier><identifier>ISSN: 1559-0291</identifier><identifier>EISSN: 1559-0291</identifier><identifier>DOI: 10.1007/s12010-021-03588-8</identifier><identifier>PMID: 34086255</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>beta-Mannosidase - biosynthesis ; beta-Mannosidase - genetics ; beta-Mannosidase - metabolism ; Biochemistry ; Biotechnology ; Carbon sources ; Cellulose ; Cellulose - metabolism ; Chemistry ; Chemistry and Materials Science ; Enzymes ; Fungal Proteins - genetics ; Fungal Proteins - metabolism ; Fungi ; Galactosidase ; Gene expression ; Gene Expression Regulation, Fungal ; Genes ; Homology ; Mannanases ; Mannans - biosynthesis ; Mannans - metabolism ; Mannosidase ; Original Article ; Talaromyces ; Talaromyces - enzymology ; Talaromyces - genetics ; Transcription Factors - genetics ; Transcription Factors - metabolism</subject><ispartof>Applied biochemistry and biotechnology, 2021-10, Vol.193 (10), p.3163-3172</ispartof><rights>The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2021</rights><rights>2021. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.</rights><rights>The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2021.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c375t-2dee867e7690055296d340e87a71c99217ef0f91caaa68c8c535d2837af98a533</citedby><cites>FETCH-LOGICAL-c375t-2dee867e7690055296d340e87a71c99217ef0f91caaa68c8c535d2837af98a533</cites><orcidid>0000-0001-7247-9442 ; 0000-0002-5283-2875 ; 0000-0003-0190-2893</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s12010-021-03588-8$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s12010-021-03588-8$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34086255$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fujii, Tatsuya</creatorcontrib><creatorcontrib>Inoue, Hiroyuki</creatorcontrib><creatorcontrib>Matsushika, Akinori</creatorcontrib><title>The Transcription Factor Gene tclB2 Regulates Mannanolytic Enzyme Production in the Fungus Talaromyces cellulolyticus</title><title>Applied biochemistry and biotechnology</title><addtitle>Appl Biochem Biotechnol</addtitle><addtitle>Appl Biochem Biotechnol</addtitle><description>The filamentous fungus
Talaromyces cellulolyticus
is a well-characterized cellulolytic and hemicellulolytic enzyme producer. In this study, the function of the
tclB2
gene, which is a homolog of the
manR
/
clrB
/
clr-2
gene in other filamentous fungi, in mannanolytic enzyme production by
T. cellulolyticus
was investigated. When a
tclB2
-disrupted strain (YDTclB) was grown in the presence of glucomannan, the production of β-mannanase, β-mannosidase, and α-galactosidase was decreased at the protein and transcriptional levels when compared to the control strain. In addition, a
tclB2
-overexpressing strain (YHTclB) showed higher β-mannanase and β-mannosidase production. When cellulose was used as a carbon source, the expression of genes encoding mannanolytic enzymes also decreased in YDTclB. These results suggested that TclB2 contributes to mannanolytic enzyme production in
T. cellulolyticus
. This work is the first study to identify a transcriptional regulator of mannanolytic enzyme genes in
T. cellulolyticus
.</description><subject>beta-Mannosidase - biosynthesis</subject><subject>beta-Mannosidase - genetics</subject><subject>beta-Mannosidase - metabolism</subject><subject>Biochemistry</subject><subject>Biotechnology</subject><subject>Carbon sources</subject><subject>Cellulose</subject><subject>Cellulose - metabolism</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Enzymes</subject><subject>Fungal Proteins - genetics</subject><subject>Fungal Proteins - metabolism</subject><subject>Fungi</subject><subject>Galactosidase</subject><subject>Gene expression</subject><subject>Gene Expression Regulation, Fungal</subject><subject>Genes</subject><subject>Homology</subject><subject>Mannanases</subject><subject>Mannans - biosynthesis</subject><subject>Mannans - metabolism</subject><subject>Mannosidase</subject><subject>Original Article</subject><subject>Talaromyces</subject><subject>Talaromyces - enzymology</subject><subject>Talaromyces - genetics</subject><subject>Transcription Factors - genetics</subject><subject>Transcription Factors - metabolism</subject><issn>0273-2289</issn><issn>1559-0291</issn><issn>1559-0291</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kc1LHDEchkNR6mr7D_RQAl56Gc3H5uuo4qqgKGV7DmnmN9uRmWSbTA7bv97o2BY8eAokz_PmhRehL5ScUELUaaaMUNIQRhvChdaN_oAWVAhTrwzdQwvCFG8Y0-YAHeb8SAhlWqiP6IAviZZMiAUq61-A18mF7FO_nfoY8Mr5KSZ8BQHw5Idzhr_DpgxugozvXAguxGE39R5fhj-7EfBDim3xL2of8FTzViVsSsZrN7gUx52voodhKMMslvwJ7XduyPD59TxCP1aX64vr5vb-6ubi7LbxXImpYS2AlgqUNIQIwYxsa3PQyinqjWFUQUc6Q71zTmqvveCiZZor1xntBOdH6Nucu03xd4E82bHPz1VcgFiyZYIrydVS04oev0EfY0mhtquUkloulZSVYjPlU8w5QWe3qR9d2llK7PModh7F1lHsyyhWV-nra3T5OUL7T_m7QgX4DOT6FDaQ_v_9TuwT7h6X-w</recordid><startdate>20211001</startdate><enddate>20211001</enddate><creator>Fujii, Tatsuya</creator><creator>Inoue, Hiroyuki</creator><creator>Matsushika, Akinori</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7ST</scope><scope>7T7</scope><scope>7TM</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>RC3</scope><scope>SOI</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-7247-9442</orcidid><orcidid>https://orcid.org/0000-0002-5283-2875</orcidid><orcidid>https://orcid.org/0000-0003-0190-2893</orcidid></search><sort><creationdate>20211001</creationdate><title>The Transcription Factor Gene tclB2 Regulates Mannanolytic Enzyme Production in the Fungus Talaromyces cellulolyticus</title><author>Fujii, Tatsuya ; Inoue, Hiroyuki ; Matsushika, Akinori</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c375t-2dee867e7690055296d340e87a71c99217ef0f91caaa68c8c535d2837af98a533</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>beta-Mannosidase - biosynthesis</topic><topic>beta-Mannosidase - genetics</topic><topic>beta-Mannosidase - metabolism</topic><topic>Biochemistry</topic><topic>Biotechnology</topic><topic>Carbon sources</topic><topic>Cellulose</topic><topic>Cellulose - metabolism</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Enzymes</topic><topic>Fungal Proteins - genetics</topic><topic>Fungal Proteins - metabolism</topic><topic>Fungi</topic><topic>Galactosidase</topic><topic>Gene expression</topic><topic>Gene Expression Regulation, Fungal</topic><topic>Genes</topic><topic>Homology</topic><topic>Mannanases</topic><topic>Mannans - biosynthesis</topic><topic>Mannans - metabolism</topic><topic>Mannosidase</topic><topic>Original Article</topic><topic>Talaromyces</topic><topic>Talaromyces - enzymology</topic><topic>Talaromyces - genetics</topic><topic>Transcription Factors - genetics</topic><topic>Transcription Factors - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fujii, Tatsuya</creatorcontrib><creatorcontrib>Inoue, Hiroyuki</creatorcontrib><creatorcontrib>Matsushika, Akinori</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Environment Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>Environment Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Applied biochemistry and biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fujii, Tatsuya</au><au>Inoue, Hiroyuki</au><au>Matsushika, Akinori</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The Transcription Factor Gene tclB2 Regulates Mannanolytic Enzyme Production in the Fungus Talaromyces cellulolyticus</atitle><jtitle>Applied biochemistry and biotechnology</jtitle><stitle>Appl Biochem Biotechnol</stitle><addtitle>Appl Biochem Biotechnol</addtitle><date>2021-10-01</date><risdate>2021</risdate><volume>193</volume><issue>10</issue><spage>3163</spage><epage>3172</epage><pages>3163-3172</pages><issn>0273-2289</issn><issn>1559-0291</issn><eissn>1559-0291</eissn><abstract>The filamentous fungus
Talaromyces cellulolyticus
is a well-characterized cellulolytic and hemicellulolytic enzyme producer. In this study, the function of the
tclB2
gene, which is a homolog of the
manR
/
clrB
/
clr-2
gene in other filamentous fungi, in mannanolytic enzyme production by
T. cellulolyticus
was investigated. When a
tclB2
-disrupted strain (YDTclB) was grown in the presence of glucomannan, the production of β-mannanase, β-mannosidase, and α-galactosidase was decreased at the protein and transcriptional levels when compared to the control strain. In addition, a
tclB2
-overexpressing strain (YHTclB) showed higher β-mannanase and β-mannosidase production. When cellulose was used as a carbon source, the expression of genes encoding mannanolytic enzymes also decreased in YDTclB. These results suggested that TclB2 contributes to mannanolytic enzyme production in
T. cellulolyticus
. This work is the first study to identify a transcriptional regulator of mannanolytic enzyme genes in
T. cellulolyticus
.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>34086255</pmid><doi>10.1007/s12010-021-03588-8</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0001-7247-9442</orcidid><orcidid>https://orcid.org/0000-0002-5283-2875</orcidid><orcidid>https://orcid.org/0000-0003-0190-2893</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0273-2289 |
| ispartof | Applied biochemistry and biotechnology, 2021-10, Vol.193 (10), p.3163-3172 |
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| language | eng |
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| subjects | beta-Mannosidase - biosynthesis beta-Mannosidase - genetics beta-Mannosidase - metabolism Biochemistry Biotechnology Carbon sources Cellulose Cellulose - metabolism Chemistry Chemistry and Materials Science Enzymes Fungal Proteins - genetics Fungal Proteins - metabolism Fungi Galactosidase Gene expression Gene Expression Regulation, Fungal Genes Homology Mannanases Mannans - biosynthesis Mannans - metabolism Mannosidase Original Article Talaromyces Talaromyces - enzymology Talaromyces - genetics Transcription Factors - genetics Transcription Factors - metabolism |
| title | The Transcription Factor Gene tclB2 Regulates Mannanolytic Enzyme Production in the Fungus Talaromyces cellulolyticus |
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