Study on effects of miR-141-3p in proliferation, migration, invasion and apoptosis of colon cancer cells by inhibiting Bcl2
Purpose This study aimed to investigate the relationship between miR-141-3p and B lymphocyte-2 gene (Bcl2) gene and its biological behavior on colon cancer cell line SW480. Methods qRT-PCR was used to detect the expression level of miR-141-3p in colon cancer tissues and adjacent tissues, as well as...
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| Veröffentlicht in: | Clinical & translational oncology 2021-12, Vol.23 (12), p.2526-2535 |
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| container_title | Clinical & translational oncology |
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| creator | Tong, S. J. Zhang, X. Y. Guo, H. F. Yang, J. Qi, Y. P. Lu, S. |
| description | Purpose
This study aimed to investigate the relationship between miR-141-3p and B lymphocyte-2 gene (Bcl2) gene and its biological behavior on colon cancer cell line SW480.
Methods
qRT-PCR was used to detect the expression level of miR-141-3p in colon cancer tissues and adjacent tissues, as well as in colon cancer cell line and normal human colonic epithelial cell line FHC. MTT assay, wound assay, and Transwell demonstrated the effects of miR-141-3p on colon cancer proliferation, migration and invasion. Targetscan7.1 predictive software and dual luciferase reporter assays were used to detect the targeted regulation of miR-141-3p on the apoptosis-related gene Bcl2. MTT assay, wound assay, Transwell and flow cytometry were used to detect the effect of Bcl2 on miR-141-3p on colon cancer proliferation, migration, invasion and apoptosis.
Results
Compared with adjacent tissues, the expression of miR-141-3p in colon cancer tissues was significantly down-regulated. Colon cancer patients with low expression of miR-141-3p had poorer prognosis. Compared with normal colonic epithelial cells, miR-141-3p expression was significantly down-regulated in colon cancer cell lines, and overexpression of miR-141-3p significantly attenuated the proliferation, migration and invasion of colon cancer cells. Knockdown of miR-141-3p significantly promoted the proliferation, migration and invasion of colon cancer cells. miR-141-3p targets the negative regulation of Bcl2. Knockdown of Bcl2 significantly attenuated the promotion of miR-141-3p inhibitor on proliferation, migration and invasion of colon cancer cells and inhibition of apoptosis. Knockdown of Bcl2 significantly enhanced the inhibition effect of miR-141-3p inhibitor on proliferation, migration and invasion of colon cancer cells.
Conclusions
In conclusion, miR-141-3p can inhibit the cancer by regulating Bcl2, and miR-141-3p has the potential to become a potential therapeutic target for colon cancer. |
| doi_str_mv | 10.1007/s12094-021-02653-2 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2537637338</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2537637338</sourcerecordid><originalsourceid>FETCH-LOGICAL-c413t-d7216ec6fd6ecc6e7e3df789183a0d3878b1592e93b316036c4dfb9342ca8c773</originalsourceid><addsrcrecordid>eNp9kEtPxSAQhYnR-P4DLgxLF1aBaaFdqvGVmJj4SNwRSuGK6YUKrcmNf170Xl26AE5mzpwwH0IHlJxQQsRpoow0ZUEYzYdXULA1tE150xRAqmp9pUlZv2yhnZTeSK5ySjfRFpSk5qyCbfT5OE7dAgePjbVGjwkHi-fuoaAlLWDAzuMhht5ZE9Xogj_OzdmvdP5Dpayw8h1WQxjGkNxPgg59LmvltYlYm75PuF1k_6tr3ej8DJ_rnu2hDav6ZPZX7y56vrp8urgp7u6vby_O7gpdUhiLTjDKjea2y7fmRhjorKgbWoMiHdSibmnVMNNAC5QT4LrsbNtAybSqtRCwi46WuXmT98mkUc5d-v6U8iZMSWYQgoMAqLOVLa06hpSisXKIbq7iQlIiv6HLJXSZocsf6JLlocNV_tTOTfc38ks5G2BpSLnlZybKtzBFn3f-L_YLzA6NBA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2537637338</pqid></control><display><type>article</type><title>Study on effects of miR-141-3p in proliferation, migration, invasion and apoptosis of colon cancer cells by inhibiting Bcl2</title><source>MEDLINE</source><source>SpringerLink Journals - AutoHoldings</source><creator>Tong, S. J. ; Zhang, X. Y. ; Guo, H. F. ; Yang, J. ; Qi, Y. P. ; Lu, S.</creator><creatorcontrib>Tong, S. J. ; Zhang, X. Y. ; Guo, H. F. ; Yang, J. ; Qi, Y. P. ; Lu, S.</creatorcontrib><description>Purpose
This study aimed to investigate the relationship between miR-141-3p and B lymphocyte-2 gene (Bcl2) gene and its biological behavior on colon cancer cell line SW480.
Methods
qRT-PCR was used to detect the expression level of miR-141-3p in colon cancer tissues and adjacent tissues, as well as in colon cancer cell line and normal human colonic epithelial cell line FHC. MTT assay, wound assay, and Transwell demonstrated the effects of miR-141-3p on colon cancer proliferation, migration and invasion. Targetscan7.1 predictive software and dual luciferase reporter assays were used to detect the targeted regulation of miR-141-3p on the apoptosis-related gene Bcl2. MTT assay, wound assay, Transwell and flow cytometry were used to detect the effect of Bcl2 on miR-141-3p on colon cancer proliferation, migration, invasion and apoptosis.
Results
Compared with adjacent tissues, the expression of miR-141-3p in colon cancer tissues was significantly down-regulated. Colon cancer patients with low expression of miR-141-3p had poorer prognosis. Compared with normal colonic epithelial cells, miR-141-3p expression was significantly down-regulated in colon cancer cell lines, and overexpression of miR-141-3p significantly attenuated the proliferation, migration and invasion of colon cancer cells. Knockdown of miR-141-3p significantly promoted the proliferation, migration and invasion of colon cancer cells. miR-141-3p targets the negative regulation of Bcl2. Knockdown of Bcl2 significantly attenuated the promotion of miR-141-3p inhibitor on proliferation, migration and invasion of colon cancer cells and inhibition of apoptosis. Knockdown of Bcl2 significantly enhanced the inhibition effect of miR-141-3p inhibitor on proliferation, migration and invasion of colon cancer cells.
Conclusions
In conclusion, miR-141-3p can inhibit the cancer by regulating Bcl2, and miR-141-3p has the potential to become a potential therapeutic target for colon cancer.</description><identifier>ISSN: 1699-048X</identifier><identifier>EISSN: 1699-3055</identifier><identifier>DOI: 10.1007/s12094-021-02653-2</identifier><identifier>PMID: 34086253</identifier><language>eng</language><publisher>Cham: Springer International Publishing</publisher><subject>Apoptosis ; Biomarkers, Tumor - genetics ; Biomarkers, Tumor - metabolism ; Cell Movement ; Cell Proliferation ; Colonic Neoplasms - genetics ; Colonic Neoplasms - metabolism ; Colonic Neoplasms - pathology ; Gene Expression Regulation, Neoplastic ; Humans ; Medicine ; Medicine & Public Health ; MicroRNAs - genetics ; Neoplasm Invasiveness ; Oncology ; Prognosis ; Proto-Oncogene Proteins c-bcl-2 - antagonists & inhibitors ; Proto-Oncogene Proteins c-bcl-2 - genetics ; Proto-Oncogene Proteins c-bcl-2 - metabolism ; Research Article ; Survival Rate ; Tumor Cells, Cultured</subject><ispartof>Clinical & translational oncology, 2021-12, Vol.23 (12), p.2526-2535</ispartof><rights>Federación de Sociedades Españolas de Oncología (FESEO) 2021</rights><rights>2021. Federación de Sociedades Españolas de Oncología (FESEO).</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c413t-d7216ec6fd6ecc6e7e3df789183a0d3878b1592e93b316036c4dfb9342ca8c773</citedby><cites>FETCH-LOGICAL-c413t-d7216ec6fd6ecc6e7e3df789183a0d3878b1592e93b316036c4dfb9342ca8c773</cites><orcidid>0000-0003-3831-8447</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s12094-021-02653-2$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s12094-021-02653-2$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34086253$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tong, S. J.</creatorcontrib><creatorcontrib>Zhang, X. Y.</creatorcontrib><creatorcontrib>Guo, H. F.</creatorcontrib><creatorcontrib>Yang, J.</creatorcontrib><creatorcontrib>Qi, Y. P.</creatorcontrib><creatorcontrib>Lu, S.</creatorcontrib><title>Study on effects of miR-141-3p in proliferation, migration, invasion and apoptosis of colon cancer cells by inhibiting Bcl2</title><title>Clinical & translational oncology</title><addtitle>Clin Transl Oncol</addtitle><addtitle>Clin Transl Oncol</addtitle><description>Purpose
This study aimed to investigate the relationship between miR-141-3p and B lymphocyte-2 gene (Bcl2) gene and its biological behavior on colon cancer cell line SW480.
Methods
qRT-PCR was used to detect the expression level of miR-141-3p in colon cancer tissues and adjacent tissues, as well as in colon cancer cell line and normal human colonic epithelial cell line FHC. MTT assay, wound assay, and Transwell demonstrated the effects of miR-141-3p on colon cancer proliferation, migration and invasion. Targetscan7.1 predictive software and dual luciferase reporter assays were used to detect the targeted regulation of miR-141-3p on the apoptosis-related gene Bcl2. MTT assay, wound assay, Transwell and flow cytometry were used to detect the effect of Bcl2 on miR-141-3p on colon cancer proliferation, migration, invasion and apoptosis.
Results
Compared with adjacent tissues, the expression of miR-141-3p in colon cancer tissues was significantly down-regulated. Colon cancer patients with low expression of miR-141-3p had poorer prognosis. Compared with normal colonic epithelial cells, miR-141-3p expression was significantly down-regulated in colon cancer cell lines, and overexpression of miR-141-3p significantly attenuated the proliferation, migration and invasion of colon cancer cells. Knockdown of miR-141-3p significantly promoted the proliferation, migration and invasion of colon cancer cells. miR-141-3p targets the negative regulation of Bcl2. Knockdown of Bcl2 significantly attenuated the promotion of miR-141-3p inhibitor on proliferation, migration and invasion of colon cancer cells and inhibition of apoptosis. Knockdown of Bcl2 significantly enhanced the inhibition effect of miR-141-3p inhibitor on proliferation, migration and invasion of colon cancer cells.
Conclusions
In conclusion, miR-141-3p can inhibit the cancer by regulating Bcl2, and miR-141-3p has the potential to become a potential therapeutic target for colon cancer.</description><subject>Apoptosis</subject><subject>Biomarkers, Tumor - genetics</subject><subject>Biomarkers, Tumor - metabolism</subject><subject>Cell Movement</subject><subject>Cell Proliferation</subject><subject>Colonic Neoplasms - genetics</subject><subject>Colonic Neoplasms - metabolism</subject><subject>Colonic Neoplasms - pathology</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>Humans</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>MicroRNAs - genetics</subject><subject>Neoplasm Invasiveness</subject><subject>Oncology</subject><subject>Prognosis</subject><subject>Proto-Oncogene Proteins c-bcl-2 - antagonists & inhibitors</subject><subject>Proto-Oncogene Proteins c-bcl-2 - genetics</subject><subject>Proto-Oncogene Proteins c-bcl-2 - metabolism</subject><subject>Research Article</subject><subject>Survival Rate</subject><subject>Tumor Cells, Cultured</subject><issn>1699-048X</issn><issn>1699-3055</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEtPxSAQhYnR-P4DLgxLF1aBaaFdqvGVmJj4SNwRSuGK6YUKrcmNf170Xl26AE5mzpwwH0IHlJxQQsRpoow0ZUEYzYdXULA1tE150xRAqmp9pUlZv2yhnZTeSK5ySjfRFpSk5qyCbfT5OE7dAgePjbVGjwkHi-fuoaAlLWDAzuMhht5ZE9Xogj_OzdmvdP5Dpayw8h1WQxjGkNxPgg59LmvltYlYm75PuF1k_6tr3ej8DJ_rnu2hDav6ZPZX7y56vrp8urgp7u6vby_O7gpdUhiLTjDKjea2y7fmRhjorKgbWoMiHdSibmnVMNNAC5QT4LrsbNtAybSqtRCwi46WuXmT98mkUc5d-v6U8iZMSWYQgoMAqLOVLa06hpSisXKIbq7iQlIiv6HLJXSZocsf6JLlocNV_tTOTfc38ks5G2BpSLnlZybKtzBFn3f-L_YLzA6NBA</recordid><startdate>20211201</startdate><enddate>20211201</enddate><creator>Tong, S. J.</creator><creator>Zhang, X. Y.</creator><creator>Guo, H. F.</creator><creator>Yang, J.</creator><creator>Qi, Y. P.</creator><creator>Lu, S.</creator><general>Springer International Publishing</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-3831-8447</orcidid></search><sort><creationdate>20211201</creationdate><title>Study on effects of miR-141-3p in proliferation, migration, invasion and apoptosis of colon cancer cells by inhibiting Bcl2</title><author>Tong, S. J. ; Zhang, X. Y. ; Guo, H. F. ; Yang, J. ; Qi, Y. P. ; Lu, S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c413t-d7216ec6fd6ecc6e7e3df789183a0d3878b1592e93b316036c4dfb9342ca8c773</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Apoptosis</topic><topic>Biomarkers, Tumor - genetics</topic><topic>Biomarkers, Tumor - metabolism</topic><topic>Cell Movement</topic><topic>Cell Proliferation</topic><topic>Colonic Neoplasms - genetics</topic><topic>Colonic Neoplasms - metabolism</topic><topic>Colonic Neoplasms - pathology</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>Humans</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>MicroRNAs - genetics</topic><topic>Neoplasm Invasiveness</topic><topic>Oncology</topic><topic>Prognosis</topic><topic>Proto-Oncogene Proteins c-bcl-2 - antagonists & inhibitors</topic><topic>Proto-Oncogene Proteins c-bcl-2 - genetics</topic><topic>Proto-Oncogene Proteins c-bcl-2 - metabolism</topic><topic>Research Article</topic><topic>Survival Rate</topic><topic>Tumor Cells, Cultured</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tong, S. J.</creatorcontrib><creatorcontrib>Zhang, X. Y.</creatorcontrib><creatorcontrib>Guo, H. F.</creatorcontrib><creatorcontrib>Yang, J.</creatorcontrib><creatorcontrib>Qi, Y. P.</creatorcontrib><creatorcontrib>Lu, S.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Clinical & translational oncology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tong, S. J.</au><au>Zhang, X. Y.</au><au>Guo, H. F.</au><au>Yang, J.</au><au>Qi, Y. P.</au><au>Lu, S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Study on effects of miR-141-3p in proliferation, migration, invasion and apoptosis of colon cancer cells by inhibiting Bcl2</atitle><jtitle>Clinical & translational oncology</jtitle><stitle>Clin Transl Oncol</stitle><addtitle>Clin Transl Oncol</addtitle><date>2021-12-01</date><risdate>2021</risdate><volume>23</volume><issue>12</issue><spage>2526</spage><epage>2535</epage><pages>2526-2535</pages><issn>1699-048X</issn><eissn>1699-3055</eissn><abstract>Purpose
This study aimed to investigate the relationship between miR-141-3p and B lymphocyte-2 gene (Bcl2) gene and its biological behavior on colon cancer cell line SW480.
Methods
qRT-PCR was used to detect the expression level of miR-141-3p in colon cancer tissues and adjacent tissues, as well as in colon cancer cell line and normal human colonic epithelial cell line FHC. MTT assay, wound assay, and Transwell demonstrated the effects of miR-141-3p on colon cancer proliferation, migration and invasion. Targetscan7.1 predictive software and dual luciferase reporter assays were used to detect the targeted regulation of miR-141-3p on the apoptosis-related gene Bcl2. MTT assay, wound assay, Transwell and flow cytometry were used to detect the effect of Bcl2 on miR-141-3p on colon cancer proliferation, migration, invasion and apoptosis.
Results
Compared with adjacent tissues, the expression of miR-141-3p in colon cancer tissues was significantly down-regulated. Colon cancer patients with low expression of miR-141-3p had poorer prognosis. Compared with normal colonic epithelial cells, miR-141-3p expression was significantly down-regulated in colon cancer cell lines, and overexpression of miR-141-3p significantly attenuated the proliferation, migration and invasion of colon cancer cells. Knockdown of miR-141-3p significantly promoted the proliferation, migration and invasion of colon cancer cells. miR-141-3p targets the negative regulation of Bcl2. Knockdown of Bcl2 significantly attenuated the promotion of miR-141-3p inhibitor on proliferation, migration and invasion of colon cancer cells and inhibition of apoptosis. Knockdown of Bcl2 significantly enhanced the inhibition effect of miR-141-3p inhibitor on proliferation, migration and invasion of colon cancer cells.
Conclusions
In conclusion, miR-141-3p can inhibit the cancer by regulating Bcl2, and miR-141-3p has the potential to become a potential therapeutic target for colon cancer.</abstract><cop>Cham</cop><pub>Springer International Publishing</pub><pmid>34086253</pmid><doi>10.1007/s12094-021-02653-2</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0003-3831-8447</orcidid></addata></record> |
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| subjects | Apoptosis Biomarkers, Tumor - genetics Biomarkers, Tumor - metabolism Cell Movement Cell Proliferation Colonic Neoplasms - genetics Colonic Neoplasms - metabolism Colonic Neoplasms - pathology Gene Expression Regulation, Neoplastic Humans Medicine Medicine & Public Health MicroRNAs - genetics Neoplasm Invasiveness Oncology Prognosis Proto-Oncogene Proteins c-bcl-2 - antagonists & inhibitors Proto-Oncogene Proteins c-bcl-2 - genetics Proto-Oncogene Proteins c-bcl-2 - metabolism Research Article Survival Rate Tumor Cells, Cultured |
| title | Study on effects of miR-141-3p in proliferation, migration, invasion and apoptosis of colon cancer cells by inhibiting Bcl2 |
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