Analysis of neuronal Ca2+ handling properties by combining perforated patch clamp recordings and the added buffer approach

[Display omitted] •Combining added buffer approach and perforated patch clamp optimizes quantification of Ca2+ handling.•β-escin as perforating agent allows controlled Ca2+ buffer loading while preserving cytoplasmic pathways.•Mobile and Immobile Ca2+ buffers can be quantified. Ca2+ functions as an...

Ausführliche Beschreibung

Bibliographische Detailangaben

Veröffentlicht in:	Cell calcium (Edinburgh) 2021-07, Vol.97, p.102411-102411, Article 102411
Hauptverfasser:	Hess, Simon, Pouzat, Christophe, Paeger, Lars, Pippow, Andreas, Kloppenburg, Peter
Format:	Artikel
Sprache:	eng
Schlagworte:	Added buffer approach Calcium buffering Calcium-binding ratio Cerebellum Fura-2 Midbrain Perforated patch clamp β-Escin
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!