Network pharmacology approach to elucidate possible action mechanisms of Sinomenii Caulis for treating osteoporosis
Sinomenii Caulis (SC) is a well-konwn traditional Chinese medicine used for treatment of rheumatoid arthritis (RA), dermatophytosis and paralysis. Patients with RA are usually secondary to osteoporosis, but the potential protective effect of SC on osteoporosis (OP) is seldom reported and its possibl...
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| Veröffentlicht in: | Journal of ethnopharmacology 2020-07, Vol.257 (NA), p.112871, Article 112871 |
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| creator | Liu, Wen-jin Jiang, Zheng-meng Chen, Yi Xiao, Ping-ting Wang, Zi-yuan Huang, Tian-qing Liu, E-hu |
| description | Sinomenii Caulis (SC) is a well-konwn traditional Chinese medicine used for treatment of rheumatoid arthritis (RA), dermatophytosis and paralysis. Patients with RA are usually secondary to osteoporosis, but the potential protective effect of SC on osteoporosis (OP) is seldom reported and its possible action mechanism is little known.
The purpose of this study was to demonstrate the anti-osteoporosis effects of SC extract and alkaloids in prednisolone (Pre)-induced OP of zebrafish, and then to explore the potential mechanism of SC on system level by network pharmacology.
Firstly, zebrafish OP model was established to investigate the anti-osteoporosis effect of SC. Secondly, the targets of SC and OP from multiple databases were collected, and Compound-Target-Pathway network based on protein-protein interaction (PPI) was constructed. Moreover, gene enrichment and annotation were performed via the DAVID server. Finally, the reliability of the network pharmacology prediction results in Pre-induced OP of zebrafish was verified by qRT-PCR.
The results indicated that SC extract and alkaloids have remarkable ability to promote bone formation of cranial bones and reduce TRAP contents in Pre-induced OP of zebrafish. 32 OP-related ingredients in SC and 77 OP-related targets were screened from multiple databases, and 15 OP-related pathways were enriched by the KEGG database. Further experimental validation indicated that SC extract and alkaloids could regulate the expression of MAPK14, CASP3, CXCL8, IL-1β, IL6, PTGS2, TNF-α, ESR1, and MMP9 for treatment of OP.
In summary, we conducted an integrative analysis to provide convincing evidence that SC may partially alleviate OP by inhibiting pro-inflammatory cytokines and regulating of RANK/RANKL/OPG system.
[Display omitted]
•C-T-P network based on protein-protein interaction (PPI) was constructed.•32 OP-related ingredients, 77 OP-related targets, and 15 pathways were enriched.•SC regulate the expression of PTGS2, IL-1β, TNF-α, and MMP9 for treatment of OP. |
| doi_str_mv | 10.1016/j.jep.2020.112871 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2536415014</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0378874120310539</els_id><sourcerecordid>2536415014</sourcerecordid><originalsourceid>FETCH-LOGICAL-c386t-d804690d9ad1a7e03d0721848e8c3be11411f8baf535763aed8e363219d1d0323</originalsourceid><addsrcrecordid>eNp9kMFu1DAQhi0EotvCA3BBPnLJ4rGT2CtOaFWgUgUH4Gx57UnXSxIHjwPq2-NqC0dOo5G--TX_x9grEFsQ0L89bU-4bKWQdQdpNDxhGzBaNrrT6inbCKVNY3QLF-yS6CSE0NCK5-xCSSU7MHLD6DOW3yn_4MvR5cn5NKa7e-6WJSfnj7wkjuPqY3AF-ZKI4mFE7nyJaeYT-qObI03E08C_xjlNOMfI924dI_EhZV4yuhLnO56oYFpSThTpBXs2uJHw5eO8Yt8_XH_bf2puv3y82b-_bbwyfWmCEW2_E2HnAjiNQgWhJZjWoPHqgAAtwGAObuhUp3vlMBhUvZKwCxBErXjF3pxza5mfK1KxUySP4-hmTCtZ2am-hU5AW1E4o75-SBkHu-Q4uXxvQdgH1_Zkq2v74NqeXdeb14_x62HC8O_ir9wKvDsDWEv-ipgt-YizxxAz-mJDiv-J_wPqZpDP</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2536415014</pqid></control><display><type>article</type><title>Network pharmacology approach to elucidate possible action mechanisms of Sinomenii Caulis for treating osteoporosis</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals Complete</source><creator>Liu, Wen-jin ; Jiang, Zheng-meng ; Chen, Yi ; Xiao, Ping-ting ; Wang, Zi-yuan ; Huang, Tian-qing ; Liu, E-hu</creator><creatorcontrib>Liu, Wen-jin ; Jiang, Zheng-meng ; Chen, Yi ; Xiao, Ping-ting ; Wang, Zi-yuan ; Huang, Tian-qing ; Liu, E-hu</creatorcontrib><description>Sinomenii Caulis (SC) is a well-konwn traditional Chinese medicine used for treatment of rheumatoid arthritis (RA), dermatophytosis and paralysis. Patients with RA are usually secondary to osteoporosis, but the potential protective effect of SC on osteoporosis (OP) is seldom reported and its possible action mechanism is little known.
The purpose of this study was to demonstrate the anti-osteoporosis effects of SC extract and alkaloids in prednisolone (Pre)-induced OP of zebrafish, and then to explore the potential mechanism of SC on system level by network pharmacology.
Firstly, zebrafish OP model was established to investigate the anti-osteoporosis effect of SC. Secondly, the targets of SC and OP from multiple databases were collected, and Compound-Target-Pathway network based on protein-protein interaction (PPI) was constructed. Moreover, gene enrichment and annotation were performed via the DAVID server. Finally, the reliability of the network pharmacology prediction results in Pre-induced OP of zebrafish was verified by qRT-PCR.
The results indicated that SC extract and alkaloids have remarkable ability to promote bone formation of cranial bones and reduce TRAP contents in Pre-induced OP of zebrafish. 32 OP-related ingredients in SC and 77 OP-related targets were screened from multiple databases, and 15 OP-related pathways were enriched by the KEGG database. Further experimental validation indicated that SC extract and alkaloids could regulate the expression of MAPK14, CASP3, CXCL8, IL-1β, IL6, PTGS2, TNF-α, ESR1, and MMP9 for treatment of OP.
In summary, we conducted an integrative analysis to provide convincing evidence that SC may partially alleviate OP by inhibiting pro-inflammatory cytokines and regulating of RANK/RANKL/OPG system.
[Display omitted]
•C-T-P network based on protein-protein interaction (PPI) was constructed.•32 OP-related ingredients, 77 OP-related targets, and 15 pathways were enriched.•SC regulate the expression of PTGS2, IL-1β, TNF-α, and MMP9 for treatment of OP.</description><identifier>ISSN: 0378-8741</identifier><identifier>EISSN: 1872-7573</identifier><identifier>DOI: 10.1016/j.jep.2020.112871</identifier><identifier>PMID: 32325182</identifier><language>eng</language><publisher>Ireland: Elsevier B.V</publisher><subject>Animals ; Anti-Inflammatory Agents - pharmacology ; Bone and Bones - drug effects ; Bone and Bones - metabolism ; Bone and Bones - physiopathology ; Bone Density Conservation Agents - pharmacology ; Bone Remodeling - drug effects ; Bone Remodeling - genetics ; Cytokines - genetics ; Cytokines - metabolism ; Databases, Protein ; Disease Models, Animal ; Drugs, Chinese Herbal - pharmacology ; Gene Expression Regulation ; Gene Regulatory Networks ; Inflammation Mediators - metabolism ; Network pharmacology ; Osteoporosis ; Osteoporosis - chemically induced ; Osteoporosis - genetics ; Osteoporosis - metabolism ; Osteoporosis - physiopathology ; Osteoprotegerin - genetics ; Osteoprotegerin - metabolism ; Prednisolone ; Protein Interaction Maps ; RANK Ligand - genetics ; RANK Ligand - metabolism ; Receptor Activator of Nuclear Factor-kappa B - genetics ; Receptor Activator of Nuclear Factor-kappa B - metabolism ; Signal Transduction ; Sinomenii caulis ; Systems Biology ; Zebrafish ; Zebrafish Proteins - genetics ; Zebrafish Proteins - metabolism</subject><ispartof>Journal of ethnopharmacology, 2020-07, Vol.257 (NA), p.112871, Article 112871</ispartof><rights>2020 Elsevier B.V.</rights><rights>Copyright © 2020 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c386t-d804690d9ad1a7e03d0721848e8c3be11411f8baf535763aed8e363219d1d0323</citedby><cites>FETCH-LOGICAL-c386t-d804690d9ad1a7e03d0721848e8c3be11411f8baf535763aed8e363219d1d0323</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0378874120310539$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32325182$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Liu, Wen-jin</creatorcontrib><creatorcontrib>Jiang, Zheng-meng</creatorcontrib><creatorcontrib>Chen, Yi</creatorcontrib><creatorcontrib>Xiao, Ping-ting</creatorcontrib><creatorcontrib>Wang, Zi-yuan</creatorcontrib><creatorcontrib>Huang, Tian-qing</creatorcontrib><creatorcontrib>Liu, E-hu</creatorcontrib><title>Network pharmacology approach to elucidate possible action mechanisms of Sinomenii Caulis for treating osteoporosis</title><title>Journal of ethnopharmacology</title><addtitle>J Ethnopharmacol</addtitle><description>Sinomenii Caulis (SC) is a well-konwn traditional Chinese medicine used for treatment of rheumatoid arthritis (RA), dermatophytosis and paralysis. Patients with RA are usually secondary to osteoporosis, but the potential protective effect of SC on osteoporosis (OP) is seldom reported and its possible action mechanism is little known.
The purpose of this study was to demonstrate the anti-osteoporosis effects of SC extract and alkaloids in prednisolone (Pre)-induced OP of zebrafish, and then to explore the potential mechanism of SC on system level by network pharmacology.
Firstly, zebrafish OP model was established to investigate the anti-osteoporosis effect of SC. Secondly, the targets of SC and OP from multiple databases were collected, and Compound-Target-Pathway network based on protein-protein interaction (PPI) was constructed. Moreover, gene enrichment and annotation were performed via the DAVID server. Finally, the reliability of the network pharmacology prediction results in Pre-induced OP of zebrafish was verified by qRT-PCR.
The results indicated that SC extract and alkaloids have remarkable ability to promote bone formation of cranial bones and reduce TRAP contents in Pre-induced OP of zebrafish. 32 OP-related ingredients in SC and 77 OP-related targets were screened from multiple databases, and 15 OP-related pathways were enriched by the KEGG database. Further experimental validation indicated that SC extract and alkaloids could regulate the expression of MAPK14, CASP3, CXCL8, IL-1β, IL6, PTGS2, TNF-α, ESR1, and MMP9 for treatment of OP.
In summary, we conducted an integrative analysis to provide convincing evidence that SC may partially alleviate OP by inhibiting pro-inflammatory cytokines and regulating of RANK/RANKL/OPG system.
[Display omitted]
•C-T-P network based on protein-protein interaction (PPI) was constructed.•32 OP-related ingredients, 77 OP-related targets, and 15 pathways were enriched.•SC regulate the expression of PTGS2, IL-1β, TNF-α, and MMP9 for treatment of OP.</description><subject>Animals</subject><subject>Anti-Inflammatory Agents - pharmacology</subject><subject>Bone and Bones - drug effects</subject><subject>Bone and Bones - metabolism</subject><subject>Bone and Bones - physiopathology</subject><subject>Bone Density Conservation Agents - pharmacology</subject><subject>Bone Remodeling - drug effects</subject><subject>Bone Remodeling - genetics</subject><subject>Cytokines - genetics</subject><subject>Cytokines - metabolism</subject><subject>Databases, Protein</subject><subject>Disease Models, Animal</subject><subject>Drugs, Chinese Herbal - pharmacology</subject><subject>Gene Expression Regulation</subject><subject>Gene Regulatory Networks</subject><subject>Inflammation Mediators - metabolism</subject><subject>Network pharmacology</subject><subject>Osteoporosis</subject><subject>Osteoporosis - chemically induced</subject><subject>Osteoporosis - genetics</subject><subject>Osteoporosis - metabolism</subject><subject>Osteoporosis - physiopathology</subject><subject>Osteoprotegerin - genetics</subject><subject>Osteoprotegerin - metabolism</subject><subject>Prednisolone</subject><subject>Protein Interaction Maps</subject><subject>RANK Ligand - genetics</subject><subject>RANK Ligand - metabolism</subject><subject>Receptor Activator of Nuclear Factor-kappa B - genetics</subject><subject>Receptor Activator of Nuclear Factor-kappa B - metabolism</subject><subject>Signal Transduction</subject><subject>Sinomenii caulis</subject><subject>Systems Biology</subject><subject>Zebrafish</subject><subject>Zebrafish Proteins - genetics</subject><subject>Zebrafish Proteins - metabolism</subject><issn>0378-8741</issn><issn>1872-7573</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMFu1DAQhi0EotvCA3BBPnLJ4rGT2CtOaFWgUgUH4Gx57UnXSxIHjwPq2-NqC0dOo5G--TX_x9grEFsQ0L89bU-4bKWQdQdpNDxhGzBaNrrT6inbCKVNY3QLF-yS6CSE0NCK5-xCSSU7MHLD6DOW3yn_4MvR5cn5NKa7e-6WJSfnj7wkjuPqY3AF-ZKI4mFE7nyJaeYT-qObI03E08C_xjlNOMfI924dI_EhZV4yuhLnO56oYFpSThTpBXs2uJHw5eO8Yt8_XH_bf2puv3y82b-_bbwyfWmCEW2_E2HnAjiNQgWhJZjWoPHqgAAtwGAObuhUp3vlMBhUvZKwCxBErXjF3pxza5mfK1KxUySP4-hmTCtZ2am-hU5AW1E4o75-SBkHu-Q4uXxvQdgH1_Zkq2v74NqeXdeb14_x62HC8O_ir9wKvDsDWEv-ipgt-YizxxAz-mJDiv-J_wPqZpDP</recordid><startdate>20200715</startdate><enddate>20200715</enddate><creator>Liu, Wen-jin</creator><creator>Jiang, Zheng-meng</creator><creator>Chen, Yi</creator><creator>Xiao, Ping-ting</creator><creator>Wang, Zi-yuan</creator><creator>Huang, Tian-qing</creator><creator>Liu, E-hu</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20200715</creationdate><title>Network pharmacology approach to elucidate possible action mechanisms of Sinomenii Caulis for treating osteoporosis</title><author>Liu, Wen-jin ; Jiang, Zheng-meng ; Chen, Yi ; Xiao, Ping-ting ; Wang, Zi-yuan ; Huang, Tian-qing ; Liu, E-hu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c386t-d804690d9ad1a7e03d0721848e8c3be11411f8baf535763aed8e363219d1d0323</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Animals</topic><topic>Anti-Inflammatory Agents - pharmacology</topic><topic>Bone and Bones - drug effects</topic><topic>Bone and Bones - metabolism</topic><topic>Bone and Bones - physiopathology</topic><topic>Bone Density Conservation Agents - pharmacology</topic><topic>Bone Remodeling - drug effects</topic><topic>Bone Remodeling - genetics</topic><topic>Cytokines - genetics</topic><topic>Cytokines - metabolism</topic><topic>Databases, Protein</topic><topic>Disease Models, Animal</topic><topic>Drugs, Chinese Herbal - pharmacology</topic><topic>Gene Expression Regulation</topic><topic>Gene Regulatory Networks</topic><topic>Inflammation Mediators - metabolism</topic><topic>Network pharmacology</topic><topic>Osteoporosis</topic><topic>Osteoporosis - chemically induced</topic><topic>Osteoporosis - genetics</topic><topic>Osteoporosis - metabolism</topic><topic>Osteoporosis - physiopathology</topic><topic>Osteoprotegerin - genetics</topic><topic>Osteoprotegerin - metabolism</topic><topic>Prednisolone</topic><topic>Protein Interaction Maps</topic><topic>RANK Ligand - genetics</topic><topic>RANK Ligand - metabolism</topic><topic>Receptor Activator of Nuclear Factor-kappa B - genetics</topic><topic>Receptor Activator of Nuclear Factor-kappa B - metabolism</topic><topic>Signal Transduction</topic><topic>Sinomenii caulis</topic><topic>Systems Biology</topic><topic>Zebrafish</topic><topic>Zebrafish Proteins - genetics</topic><topic>Zebrafish Proteins - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liu, Wen-jin</creatorcontrib><creatorcontrib>Jiang, Zheng-meng</creatorcontrib><creatorcontrib>Chen, Yi</creatorcontrib><creatorcontrib>Xiao, Ping-ting</creatorcontrib><creatorcontrib>Wang, Zi-yuan</creatorcontrib><creatorcontrib>Huang, Tian-qing</creatorcontrib><creatorcontrib>Liu, E-hu</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>Journal of ethnopharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liu, Wen-jin</au><au>Jiang, Zheng-meng</au><au>Chen, Yi</au><au>Xiao, Ping-ting</au><au>Wang, Zi-yuan</au><au>Huang, Tian-qing</au><au>Liu, E-hu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Network pharmacology approach to elucidate possible action mechanisms of Sinomenii Caulis for treating osteoporosis</atitle><jtitle>Journal of ethnopharmacology</jtitle><addtitle>J Ethnopharmacol</addtitle><date>2020-07-15</date><risdate>2020</risdate><volume>257</volume><issue>NA</issue><spage>112871</spage><pages>112871-</pages><artnum>112871</artnum><issn>0378-8741</issn><eissn>1872-7573</eissn><abstract>Sinomenii Caulis (SC) is a well-konwn traditional Chinese medicine used for treatment of rheumatoid arthritis (RA), dermatophytosis and paralysis. Patients with RA are usually secondary to osteoporosis, but the potential protective effect of SC on osteoporosis (OP) is seldom reported and its possible action mechanism is little known.
The purpose of this study was to demonstrate the anti-osteoporosis effects of SC extract and alkaloids in prednisolone (Pre)-induced OP of zebrafish, and then to explore the potential mechanism of SC on system level by network pharmacology.
Firstly, zebrafish OP model was established to investigate the anti-osteoporosis effect of SC. Secondly, the targets of SC and OP from multiple databases were collected, and Compound-Target-Pathway network based on protein-protein interaction (PPI) was constructed. Moreover, gene enrichment and annotation were performed via the DAVID server. Finally, the reliability of the network pharmacology prediction results in Pre-induced OP of zebrafish was verified by qRT-PCR.
The results indicated that SC extract and alkaloids have remarkable ability to promote bone formation of cranial bones and reduce TRAP contents in Pre-induced OP of zebrafish. 32 OP-related ingredients in SC and 77 OP-related targets were screened from multiple databases, and 15 OP-related pathways were enriched by the KEGG database. Further experimental validation indicated that SC extract and alkaloids could regulate the expression of MAPK14, CASP3, CXCL8, IL-1β, IL6, PTGS2, TNF-α, ESR1, and MMP9 for treatment of OP.
In summary, we conducted an integrative analysis to provide convincing evidence that SC may partially alleviate OP by inhibiting pro-inflammatory cytokines and regulating of RANK/RANKL/OPG system.
[Display omitted]
•C-T-P network based on protein-protein interaction (PPI) was constructed.•32 OP-related ingredients, 77 OP-related targets, and 15 pathways were enriched.•SC regulate the expression of PTGS2, IL-1β, TNF-α, and MMP9 for treatment of OP.</abstract><cop>Ireland</cop><pub>Elsevier B.V</pub><pmid>32325182</pmid><doi>10.1016/j.jep.2020.112871</doi></addata></record> |
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| subjects | Animals Anti-Inflammatory Agents - pharmacology Bone and Bones - drug effects Bone and Bones - metabolism Bone and Bones - physiopathology Bone Density Conservation Agents - pharmacology Bone Remodeling - drug effects Bone Remodeling - genetics Cytokines - genetics Cytokines - metabolism Databases, Protein Disease Models, Animal Drugs, Chinese Herbal - pharmacology Gene Expression Regulation Gene Regulatory Networks Inflammation Mediators - metabolism Network pharmacology Osteoporosis Osteoporosis - chemically induced Osteoporosis - genetics Osteoporosis - metabolism Osteoporosis - physiopathology Osteoprotegerin - genetics Osteoprotegerin - metabolism Prednisolone Protein Interaction Maps RANK Ligand - genetics RANK Ligand - metabolism Receptor Activator of Nuclear Factor-kappa B - genetics Receptor Activator of Nuclear Factor-kappa B - metabolism Signal Transduction Sinomenii caulis Systems Biology Zebrafish Zebrafish Proteins - genetics Zebrafish Proteins - metabolism |
| title | Network pharmacology approach to elucidate possible action mechanisms of Sinomenii Caulis for treating osteoporosis |
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