A GRA2 minimal promoter improves the efficiency of TATi / Tet-Off conditional regulation of gene expression in Toxoplasma gondii

•The Tet-Off / TATi gene regulation technique in T. gondii was improved with a GRA2 minimal promoter.•The Signal to Noise Ratio of a regulated reporter gene increased 23-fold.•Gene expression without TATi revealed biphasic background activity of the SAG1 minimal promoter. A tetracycline-responsive t...

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Veröffentlicht in:	Molecular and biochemical parasitology 2021-07, Vol.244, p.111384-111384, Article 111384
Hauptverfasser:	Sharifpour, Mohammad Farouq, Khadiv, Shadi, Meissner, Markus, McAllister, Milton M.
Format:	Artikel
Sprache:	eng
Schlagworte:	Apicomplexa Gene control Improvement Minimal promoter Regulation Signal to Noise Ratio TATi Tet-Off Transcription
Online-Zugang:	Volltext
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