Bio-inspired PtNPs/Graphene nanocomposite based electrocatalytic sensing of metabolites of dipyrone

Noble metal nanoparticles are known to electrocatalyze various redox reactions by improving the electron transfer kinetics. In the present study, we have introduced a facile bioinspired synthesis of PtNPs and their integration for the formation of PtNPs/graphene nanocomposite using Psidium guajava (guava) leaves extract. Graphene used in nanocomposite formulation was synthesized by exfoliation of graphite in water/acetone (25:75 v/v) mixture followed by mechanical shearing using ultrasonication and microwave irradiation. PtNPs/graphene nanocomposite was drop-cast onto a glassy carbon electrode (GCE, 3 mm dia). The electrocatalytic activity of PtNPs/graphene nanocomposite was tested in a three-electrode system for sensing of metabolic products of dipyrone (DIP) formed through 1 e− and 2 e− transfer reactions. The modified electrode exhibited almost 50% reduction in electrode resistance. The limit of detection was found to be 0.142 μM with sensitivities of 0.820 and 0.445 μA․μM−1cm−2 for DIP concentration below and above 100 μM, respectively, using square wave voltammetry. The signal of sensing of metabolites of DIP was almost invariant in the presence of glucose, dopamine, uric acid, and ciprofloxacin; however, the response current was decayed by 20% within the 10th cycle. The sensing of DIP spiked in treated sewage-water and running tap-water samples was ∼100% recoverable and comparable with HPLC. [Display omitted] •PtNPs/graphene nanocomposite synthesis via green methodology using analytes found in P. guajava leaves.•Electrocatalytic sensing of dipyrone drug and its metabolites formed on PtNPs/graphene/glassy carbon electrode.•Sensitivity of dipyrone sensing of 0.820–0.445μA.μM−1cm−2 using square wave voltammetry.•Highly selective dipyrone sensing response at modified electrode in presence of common interferents.•Completely recoverable dipyrone sensing even after spiking with sewage-water and tap-water.