ASPM is a Novel Candidate Gene Associated with Colorectal Cancer Cell Growth
Colorectal cancer (CRC) is one of the most prevalent diseases worldwide; however, the molecular mechanisms involved in CRC remain unclear. Thus, we aimed to explore a novel biomarker for CRC. In this study, we screened 361 differentially expressed genes; 152 downregulated genes; and 209 upregulated...
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Veröffentlicht in: | DNA and cell biology 2021-07, Vol.40 (7), p.921-935 |
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description | Colorectal cancer (CRC) is one of the most prevalent diseases worldwide; however, the molecular mechanisms involved in CRC remain unclear. Thus, we aimed to explore a novel biomarker for CRC. In this study, we screened 361 differentially expressed genes; 152 downregulated genes; and 209 upregulated genes) through analysis of the GSE44861, GSE110223, GSE110224, and GSE113513 CRC datasets. Next, ASPM, CCNA2, CCNB1, CEP55, KIF20A, MAD2L1, MELK, RRM2, TOP2A, TPX2, TRIP13, and TTK were identified as hub genes associated with the cell cycle in CRC through comprehensive bioinformatics analysis using the Cytoscape and Metascape software, the Database for Annotation, Visualization, and Integrated Discovery (DAVID), and the Oncomine and Gene Expression Profiling Interactive Analysis 2 (GEPIA2) databases. Furthermore, ASPM mRNA expression in CRC tissues was verified in Oncomine, The Cancer Genome Atlas and our data, and ASPM was found to be significantly upregulated in CRC tissues compared with that in the noncancer colon tissues. Functionally, we showed that overexpression of ASPM significantly promoted the proliferation and inhibited apoptosis; silencing of ASPM suppressed the proliferation of CRC cells by affecting the cell cycle G1/S transition by reducing cyclin E1 expression, and inducing apoptosis. Overall, our findings indicated that ASPM plays a crucial role in the regulation of CRC cell proliferation, and ASPM is a potential candidate diagnostic tool and therapeutic target for CRC. |
doi_str_mv | 10.1089/dna.2020.6457 |
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Thus, we aimed to explore a novel biomarker for CRC. In this study, we screened 361 differentially expressed genes; 152 downregulated genes; and 209 upregulated genes) through analysis of the GSE44861, GSE110223, GSE110224, and GSE113513 CRC datasets. Next, ASPM, CCNA2, CCNB1, CEP55, KIF20A, MAD2L1, MELK, RRM2, TOP2A, TPX2, TRIP13, and TTK were identified as hub genes associated with the cell cycle in CRC through comprehensive bioinformatics analysis using the Cytoscape and Metascape software, the Database for Annotation, Visualization, and Integrated Discovery (DAVID), and the Oncomine and Gene Expression Profiling Interactive Analysis 2 (GEPIA2) databases. Furthermore, ASPM mRNA expression in CRC tissues was verified in Oncomine, The Cancer Genome Atlas and our data, and ASPM was found to be significantly upregulated in CRC tissues compared with that in the noncancer colon tissues. Functionally, we showed that overexpression of ASPM significantly promoted the proliferation and inhibited apoptosis; silencing of ASPM suppressed the proliferation of CRC cells by affecting the cell cycle G1/S transition by reducing cyclin E1 expression, and inducing apoptosis. Overall, our findings indicated that ASPM plays a crucial role in the regulation of CRC cell proliferation, and ASPM is a potential candidate diagnostic tool and therapeutic target for CRC.</description><identifier>ISSN: 1044-5498</identifier><identifier>EISSN: 1557-7430</identifier><identifier>DOI: 10.1089/dna.2020.6457</identifier><language>eng</language><publisher>New Rochelle: Mary Ann Liebert, Inc</publisher><subject>Annotations ; Apoptosis ; Bioinformatics ; Biomarkers ; Cancer ; Cell cycle ; Cell growth ; Cell proliferation ; Colon ; Colorectal cancer ; Colorectal carcinoma ; Gene expression ; Genes ; Genomes ; Molecular modelling ; Tissues</subject><ispartof>DNA and cell biology, 2021-07, Vol.40 (7), p.921-935</ispartof><rights>Copyright Mary Ann Liebert, Inc. Jul 2021</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c298t-ec9468047d6937aa5e88cd96c4582b24e5ff5634da5342e6902af8103a6b711b3</citedby><cites>FETCH-LOGICAL-c298t-ec9468047d6937aa5e88cd96c4582b24e5ff5634da5342e6902af8103a6b711b3</cites><orcidid>0000-0002-4228-6508</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>Yang, Qian</creatorcontrib><creatorcontrib>Qi, Mingming</creatorcontrib><creatorcontrib>Chen, Yongyu</creatorcontrib><creatorcontrib>Tian, Shan</creatorcontrib><creatorcontrib>Liao, Fei</creatorcontrib><creatorcontrib>Dong, Weiguo</creatorcontrib><title>ASPM is a Novel Candidate Gene Associated with Colorectal Cancer Cell Growth</title><title>DNA and cell biology</title><description>Colorectal cancer (CRC) is one of the most prevalent diseases worldwide; however, the molecular mechanisms involved in CRC remain unclear. Thus, we aimed to explore a novel biomarker for CRC. In this study, we screened 361 differentially expressed genes; 152 downregulated genes; and 209 upregulated genes) through analysis of the GSE44861, GSE110223, GSE110224, and GSE113513 CRC datasets. Next, ASPM, CCNA2, CCNB1, CEP55, KIF20A, MAD2L1, MELK, RRM2, TOP2A, TPX2, TRIP13, and TTK were identified as hub genes associated with the cell cycle in CRC through comprehensive bioinformatics analysis using the Cytoscape and Metascape software, the Database for Annotation, Visualization, and Integrated Discovery (DAVID), and the Oncomine and Gene Expression Profiling Interactive Analysis 2 (GEPIA2) databases. Furthermore, ASPM mRNA expression in CRC tissues was verified in Oncomine, The Cancer Genome Atlas and our data, and ASPM was found to be significantly upregulated in CRC tissues compared with that in the noncancer colon tissues. Functionally, we showed that overexpression of ASPM significantly promoted the proliferation and inhibited apoptosis; silencing of ASPM suppressed the proliferation of CRC cells by affecting the cell cycle G1/S transition by reducing cyclin E1 expression, and inducing apoptosis. Overall, our findings indicated that ASPM plays a crucial role in the regulation of CRC cell proliferation, and ASPM is a potential candidate diagnostic tool and therapeutic target for CRC.</description><subject>Annotations</subject><subject>Apoptosis</subject><subject>Bioinformatics</subject><subject>Biomarkers</subject><subject>Cancer</subject><subject>Cell cycle</subject><subject>Cell growth</subject><subject>Cell proliferation</subject><subject>Colon</subject><subject>Colorectal cancer</subject><subject>Colorectal carcinoma</subject><subject>Gene expression</subject><subject>Genes</subject><subject>Genomes</subject><subject>Molecular modelling</subject><subject>Tissues</subject><issn>1044-5498</issn><issn>1557-7430</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNpdkEtLAzEUhYMoWKtL9wE3bqbmOUmWZdAq1Aeo65Bm7tAp00lNppb-e1PrytW5Bz4uhw-ha0omlGhzV_duwggjk1JIdYJGVEpVKMHJab6JEIUURp-ji5RWhBDJKBmh-fT97Rm3CTv8Er6hw5Xr67Z2A-AZ9ICnKQXf5lrjXTsscRW6EMEP7pf0EHEFXYdnMeyG5SU6a1yX4Oovx-jz4f6jeizmr7OnajovPDN6KMAbUWoiVF0arpyToLWvTemF1GzBBMimkSUXtZNcMCgNYa7RlHBXLhSlCz5Gt8e_mxi-tpAGu26TzztcD2GbLJOccyq44hm9-Yeuwjb2eV2mJCfMGCUzVRwpH0NKERq7ie3axb2lxB7c2uzWHtzag1v-A15fabI</recordid><startdate>20210701</startdate><enddate>20210701</enddate><creator>Yang, Qian</creator><creator>Qi, Mingming</creator><creator>Chen, Yongyu</creator><creator>Tian, Shan</creator><creator>Liao, Fei</creator><creator>Dong, Weiguo</creator><general>Mary Ann Liebert, Inc</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-4228-6508</orcidid></search><sort><creationdate>20210701</creationdate><title>ASPM is a Novel Candidate Gene Associated with Colorectal Cancer Cell Growth</title><author>Yang, Qian ; Qi, Mingming ; Chen, Yongyu ; Tian, Shan ; Liao, Fei ; Dong, Weiguo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c298t-ec9468047d6937aa5e88cd96c4582b24e5ff5634da5342e6902af8103a6b711b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Annotations</topic><topic>Apoptosis</topic><topic>Bioinformatics</topic><topic>Biomarkers</topic><topic>Cancer</topic><topic>Cell cycle</topic><topic>Cell growth</topic><topic>Cell proliferation</topic><topic>Colon</topic><topic>Colorectal cancer</topic><topic>Colorectal carcinoma</topic><topic>Gene expression</topic><topic>Genes</topic><topic>Genomes</topic><topic>Molecular modelling</topic><topic>Tissues</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yang, Qian</creatorcontrib><creatorcontrib>Qi, Mingming</creatorcontrib><creatorcontrib>Chen, Yongyu</creatorcontrib><creatorcontrib>Tian, Shan</creatorcontrib><creatorcontrib>Liao, Fei</creatorcontrib><creatorcontrib>Dong, Weiguo</creatorcontrib><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>DNA and cell biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yang, Qian</au><au>Qi, Mingming</au><au>Chen, Yongyu</au><au>Tian, Shan</au><au>Liao, Fei</au><au>Dong, Weiguo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>ASPM is a Novel Candidate Gene Associated with Colorectal Cancer Cell Growth</atitle><jtitle>DNA and cell biology</jtitle><date>2021-07-01</date><risdate>2021</risdate><volume>40</volume><issue>7</issue><spage>921</spage><epage>935</epage><pages>921-935</pages><issn>1044-5498</issn><eissn>1557-7430</eissn><abstract>Colorectal cancer (CRC) is one of the most prevalent diseases worldwide; however, the molecular mechanisms involved in CRC remain unclear. Thus, we aimed to explore a novel biomarker for CRC. In this study, we screened 361 differentially expressed genes; 152 downregulated genes; and 209 upregulated genes) through analysis of the GSE44861, GSE110223, GSE110224, and GSE113513 CRC datasets. Next, ASPM, CCNA2, CCNB1, CEP55, KIF20A, MAD2L1, MELK, RRM2, TOP2A, TPX2, TRIP13, and TTK were identified as hub genes associated with the cell cycle in CRC through comprehensive bioinformatics analysis using the Cytoscape and Metascape software, the Database for Annotation, Visualization, and Integrated Discovery (DAVID), and the Oncomine and Gene Expression Profiling Interactive Analysis 2 (GEPIA2) databases. Furthermore, ASPM mRNA expression in CRC tissues was verified in Oncomine, The Cancer Genome Atlas and our data, and ASPM was found to be significantly upregulated in CRC tissues compared with that in the noncancer colon tissues. Functionally, we showed that overexpression of ASPM significantly promoted the proliferation and inhibited apoptosis; silencing of ASPM suppressed the proliferation of CRC cells by affecting the cell cycle G1/S transition by reducing cyclin E1 expression, and inducing apoptosis. Overall, our findings indicated that ASPM plays a crucial role in the regulation of CRC cell proliferation, and ASPM is a potential candidate diagnostic tool and therapeutic target for CRC.</abstract><cop>New Rochelle</cop><pub>Mary Ann Liebert, Inc</pub><doi>10.1089/dna.2020.6457</doi><tpages>15</tpages><orcidid>https://orcid.org/0000-0002-4228-6508</orcidid></addata></record> |
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subjects | Annotations Apoptosis Bioinformatics Biomarkers Cancer Cell cycle Cell growth Cell proliferation Colon Colorectal cancer Colorectal carcinoma Gene expression Genes Genomes Molecular modelling Tissues |
title | ASPM is a Novel Candidate Gene Associated with Colorectal Cancer Cell Growth |
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