β-Defensin from the Asian Sea Bass, Lates calcarifer: Molecular Prediction and Phylogenetic Analysis
Antimicrobial peptides (AMPs) are an important element of the innate immune system of all living organisms and serve as a barrier that safeguards the organisms against a wide range of pathogens. Fishes are proven to be a prospective source of AMPs, and β-defensins form an important family of AMPs wi...
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| Veröffentlicht in: | Probiotics and antimicrobial proteins 2021-12, Vol.13 (6), p.1798-1807 |
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| container_title | Probiotics and antimicrobial proteins |
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| creator | Raveendran, Athira L., Dhanya Lenin K. M.V., Anju S., Neelima V.V., Anooja P., Athira P. K., Archana Philip, Rosamma Antony, Swapna P. |
| description | Antimicrobial peptides (AMPs) are an important element of the innate immune system of all living organisms and serve as a barrier that safeguards the organisms against a wide range of pathogens. Fishes are proven to be a prospective source of AMPs, and β-defensins form an important family of AMPs with potent antimicrobial, chemotactic and immunomodulatory activities. The present study reports a β-defensin AMP sequence (
Lc
-BD) from the Asian sea bass,
Lates calcarifer
, a commercially important fish species in tropical and subtropical regions of Asia and the Pacific. A 202-bp cDNA fragment with an open reading frame encoding 63 amino acids (aa) was obtained from the mRNA of gill tissue by RT-PCR. The deduced aa sequence of
Lc
-BD possessed a signal and a mature peptide region with 20 and 43 aa residues, respectively.
Lc
-BD was characterized at the molecular level, and a molecular weight of 5.24 kDa and a net charge of +4.5 was predicted for the mature peptide. The molecular characterization of
Lc
-BD revealed the presence of three intramolecular disulphide bonds involving the six conserved cysteine residues in the sequence, and the phylogenetic analysis of
Lc
-BD showed a close relationship with β-defensins from fishes like
Siniperca chuatsi
,
Argyrosomus regius
,
Trachinotus ovatus
and
Oplegnathus fasciatus. |
| doi_str_mv | 10.1007/s12602-021-09804-5 |
| format | Article |
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Lc
-BD) from the Asian sea bass,
Lates calcarifer
, a commercially important fish species in tropical and subtropical regions of Asia and the Pacific. A 202-bp cDNA fragment with an open reading frame encoding 63 amino acids (aa) was obtained from the mRNA of gill tissue by RT-PCR. The deduced aa sequence of
Lc
-BD possessed a signal and a mature peptide region with 20 and 43 aa residues, respectively.
Lc
-BD was characterized at the molecular level, and a molecular weight of 5.24 kDa and a net charge of +4.5 was predicted for the mature peptide. The molecular characterization of
Lc
-BD revealed the presence of three intramolecular disulphide bonds involving the six conserved cysteine residues in the sequence, and the phylogenetic analysis of
Lc
-BD showed a close relationship with β-defensins from fishes like
Siniperca chuatsi
,
Argyrosomus regius
,
Trachinotus ovatus
and
Oplegnathus fasciatus.</description><identifier>ISSN: 1867-1306</identifier><identifier>EISSN: 1867-1314</identifier><identifier>DOI: 10.1007/s12602-021-09804-5</identifier><identifier>PMID: 34043156</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Amino Acids ; Animals ; Antimicrobial peptides ; Applied Microbiology ; beta-Defensins - genetics ; Chemistry ; Chemistry and Materials Science ; Chemistry/Food Science ; Conserved sequence ; Defensins ; Immune system ; Immunomodulation ; Innate immunity ; Lates calcarifer ; Microbiology ; Molecular weight ; mRNA ; Nutrition ; Peptides ; Perciformes - genetics ; Phylogenetics ; Phylogeny ; Polymerase chain reaction ; Prospective Studies ; Protein Science</subject><ispartof>Probiotics and antimicrobial proteins, 2021-12, Vol.13 (6), p.1798-1807</ispartof><rights>The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2021</rights><rights>2021. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.</rights><rights>The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2021.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c375t-f1dd945fd80b29baa8205df471cd977ff6be590b42ee57738d3347dcb8af5f6d3</citedby><cites>FETCH-LOGICAL-c375t-f1dd945fd80b29baa8205df471cd977ff6be590b42ee57738d3347dcb8af5f6d3</cites><orcidid>0000-0002-9107-5897</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s12602-021-09804-5$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s12602-021-09804-5$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34043156$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Raveendran, Athira</creatorcontrib><creatorcontrib>L., Dhanya Lenin K.</creatorcontrib><creatorcontrib>M.V., Anju</creatorcontrib><creatorcontrib>S., Neelima</creatorcontrib><creatorcontrib>V.V., Anooja</creatorcontrib><creatorcontrib>P., Athira P.</creatorcontrib><creatorcontrib>K., Archana</creatorcontrib><creatorcontrib>Philip, Rosamma</creatorcontrib><creatorcontrib>Antony, Swapna P.</creatorcontrib><title>β-Defensin from the Asian Sea Bass, Lates calcarifer: Molecular Prediction and Phylogenetic Analysis</title><title>Probiotics and antimicrobial proteins</title><addtitle>Probiotics & Antimicro. Prot</addtitle><addtitle>Probiotics Antimicrob Proteins</addtitle><description>Antimicrobial peptides (AMPs) are an important element of the innate immune system of all living organisms and serve as a barrier that safeguards the organisms against a wide range of pathogens. Fishes are proven to be a prospective source of AMPs, and β-defensins form an important family of AMPs with potent antimicrobial, chemotactic and immunomodulatory activities. The present study reports a β-defensin AMP sequence (
Lc
-BD) from the Asian sea bass,
Lates calcarifer
, a commercially important fish species in tropical and subtropical regions of Asia and the Pacific. A 202-bp cDNA fragment with an open reading frame encoding 63 amino acids (aa) was obtained from the mRNA of gill tissue by RT-PCR. The deduced aa sequence of
Lc
-BD possessed a signal and a mature peptide region with 20 and 43 aa residues, respectively.
Lc
-BD was characterized at the molecular level, and a molecular weight of 5.24 kDa and a net charge of +4.5 was predicted for the mature peptide. The molecular characterization of
Lc
-BD revealed the presence of three intramolecular disulphide bonds involving the six conserved cysteine residues in the sequence, and the phylogenetic analysis of
Lc
-BD showed a close relationship with β-defensins from fishes like
Siniperca chuatsi
,
Argyrosomus regius
,
Trachinotus ovatus
and
Oplegnathus fasciatus.</description><subject>Amino Acids</subject><subject>Animals</subject><subject>Antimicrobial peptides</subject><subject>Applied Microbiology</subject><subject>beta-Defensins - genetics</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Chemistry/Food Science</subject><subject>Conserved sequence</subject><subject>Defensins</subject><subject>Immune system</subject><subject>Immunomodulation</subject><subject>Innate immunity</subject><subject>Lates calcarifer</subject><subject>Microbiology</subject><subject>Molecular weight</subject><subject>mRNA</subject><subject>Nutrition</subject><subject>Peptides</subject><subject>Perciformes - genetics</subject><subject>Phylogenetics</subject><subject>Phylogeny</subject><subject>Polymerase chain reaction</subject><subject>Prospective Studies</subject><subject>Protein Science</subject><issn>1867-1306</issn><issn>1867-1314</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp9kc1u1TAQhS0EoqXwAiyQJTYsCIz_4oTdpfxKF1EJWFuOPW5d5TrFkyzua_EgPBMptxSJBasZab5zZjSHsccCXggA-5KEbEE2IEUDfQe6MXfYseha2wgl9N3bHtoj9oDoEqBtlYT77Ehp0EqY9pjhzx_NG0xYKBee6rTj8wXyDWVf-Bf0_LUnes63fkbiwY_B15ywvuKfphHDMvrKzyrGHOY8Fe5L5GcX-3E6x4JzDnxT_LinTA_ZveRHwkc39YR9e_f26-mHZvv5_cfTzbYJypq5SSLGXpsUOxhkP3jfSTAxaStC7K1NqR3Q9DBoiWisVV1UStsYhs4nk9qoTtizg-9Vnb4vSLPbZQo4jr7gtJCTRiklhNX9ij79B72clrree031RoHttV4peaBCnYgqJndV887XvRPgrkNwhxDcGoL7HYIzq-jJjfUy7DDeSv58fQXUAaB1VM6x_t39H9tfE_2SYQ</recordid><startdate>20211201</startdate><enddate>20211201</enddate><creator>Raveendran, Athira</creator><creator>L., Dhanya Lenin K.</creator><creator>M.V., Anju</creator><creator>S., Neelima</creator><creator>V.V., Anooja</creator><creator>P., Athira P.</creator><creator>K., Archana</creator><creator>Philip, Rosamma</creator><creator>Antony, Swapna P.</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FE</scope><scope>8FH</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-9107-5897</orcidid></search><sort><creationdate>20211201</creationdate><title>β-Defensin from the Asian Sea Bass, Lates calcarifer: Molecular Prediction and Phylogenetic Analysis</title><author>Raveendran, Athira ; L., Dhanya Lenin K. ; M.V., Anju ; S., Neelima ; V.V., Anooja ; P., Athira P. ; K., Archana ; Philip, Rosamma ; Antony, Swapna P.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c375t-f1dd945fd80b29baa8205df471cd977ff6be590b42ee57738d3347dcb8af5f6d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Amino Acids</topic><topic>Animals</topic><topic>Antimicrobial peptides</topic><topic>Applied Microbiology</topic><topic>beta-Defensins - genetics</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Chemistry/Food Science</topic><topic>Conserved sequence</topic><topic>Defensins</topic><topic>Immune system</topic><topic>Immunomodulation</topic><topic>Innate immunity</topic><topic>Lates calcarifer</topic><topic>Microbiology</topic><topic>Molecular weight</topic><topic>mRNA</topic><topic>Nutrition</topic><topic>Peptides</topic><topic>Perciformes - genetics</topic><topic>Phylogenetics</topic><topic>Phylogeny</topic><topic>Polymerase chain reaction</topic><topic>Prospective Studies</topic><topic>Protein Science</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Raveendran, Athira</creatorcontrib><creatorcontrib>L., Dhanya Lenin K.</creatorcontrib><creatorcontrib>M.V., Anju</creatorcontrib><creatorcontrib>S., Neelima</creatorcontrib><creatorcontrib>V.V., Anooja</creatorcontrib><creatorcontrib>P., Athira P.</creatorcontrib><creatorcontrib>K., Archana</creatorcontrib><creatorcontrib>Philip, Rosamma</creatorcontrib><creatorcontrib>Antony, Swapna P.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>Biological Sciences</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>MEDLINE - Academic</collection><jtitle>Probiotics and antimicrobial proteins</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Raveendran, Athira</au><au>L., Dhanya Lenin K.</au><au>M.V., Anju</au><au>S., Neelima</au><au>V.V., Anooja</au><au>P., Athira P.</au><au>K., Archana</au><au>Philip, Rosamma</au><au>Antony, Swapna P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>β-Defensin from the Asian Sea Bass, Lates calcarifer: Molecular Prediction and Phylogenetic Analysis</atitle><jtitle>Probiotics and antimicrobial proteins</jtitle><stitle>Probiotics & Antimicro. Prot</stitle><addtitle>Probiotics Antimicrob Proteins</addtitle><date>2021-12-01</date><risdate>2021</risdate><volume>13</volume><issue>6</issue><spage>1798</spage><epage>1807</epage><pages>1798-1807</pages><issn>1867-1306</issn><eissn>1867-1314</eissn><abstract>Antimicrobial peptides (AMPs) are an important element of the innate immune system of all living organisms and serve as a barrier that safeguards the organisms against a wide range of pathogens. Fishes are proven to be a prospective source of AMPs, and β-defensins form an important family of AMPs with potent antimicrobial, chemotactic and immunomodulatory activities. The present study reports a β-defensin AMP sequence (
Lc
-BD) from the Asian sea bass,
Lates calcarifer
, a commercially important fish species in tropical and subtropical regions of Asia and the Pacific. A 202-bp cDNA fragment with an open reading frame encoding 63 amino acids (aa) was obtained from the mRNA of gill tissue by RT-PCR. The deduced aa sequence of
Lc
-BD possessed a signal and a mature peptide region with 20 and 43 aa residues, respectively.
Lc
-BD was characterized at the molecular level, and a molecular weight of 5.24 kDa and a net charge of +4.5 was predicted for the mature peptide. The molecular characterization of
Lc
-BD revealed the presence of three intramolecular disulphide bonds involving the six conserved cysteine residues in the sequence, and the phylogenetic analysis of
Lc
-BD showed a close relationship with β-defensins from fishes like
Siniperca chuatsi
,
Argyrosomus regius
,
Trachinotus ovatus
and
Oplegnathus fasciatus.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>34043156</pmid><doi>10.1007/s12602-021-09804-5</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0002-9107-5897</orcidid></addata></record> |
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| source | MEDLINE; SpringerLink |
| subjects | Amino Acids Animals Antimicrobial peptides Applied Microbiology beta-Defensins - genetics Chemistry Chemistry and Materials Science Chemistry/Food Science Conserved sequence Defensins Immune system Immunomodulation Innate immunity Lates calcarifer Microbiology Molecular weight mRNA Nutrition Peptides Perciformes - genetics Phylogenetics Phylogeny Polymerase chain reaction Prospective Studies Protein Science |
| title | β-Defensin from the Asian Sea Bass, Lates calcarifer: Molecular Prediction and Phylogenetic Analysis |
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