Profiling of circular RNAs and circTPCN/miR-634/mTOR regulatory pathway in cervical cancer
Circular RNAs (circRNAs) are highly stable forms of endogenous non-coding RNA molecules with diverse biological functions. Some of them have been demonstrated to play crucial roles in the initiation or development of cancers through regulation of gene expression. However, the profiles and the roles...
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| Veröffentlicht in: | Genomics (San Diego, Calif.) Calif.), 2021-07, Vol.113 (4), p.2253-2263 |
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| creator | Tian-zhao, Du Yang, Yang Xing-xuan, Wang Yu-xin, Che Xue-lian, Wang |
| description | Circular RNAs (circRNAs) are highly stable forms of endogenous non-coding RNA molecules with diverse biological functions. Some of them have been demonstrated to play crucial roles in the initiation or development of cancers through regulation of gene expression. However, the profiles and the roles of circRNAs in tumorigenesis of cervical cancer remain largely unknown. In the current study, we investigated the expression profiles of circRNAs and their potential oncogenic mechanisms in cervical cancer. The expression patterns, obtained using a microarray assay, revealed a total of 192 differentially expressed circRNAs, of which 106 were upregulated and 86 were downregulated, in cervical cancer samples compared with normal cervical samples. The differential expression of circRNAs was validated using quantitative real-time polymerase chain reaction. Two circRNAs (circTPCN and circFAM185A) were confirmed to be significantly upregulated in cervical cancer samples, indicating that they represent potential biomarkers of cervical cancer. The role and the potential molecular mechanism of circTPCN in cervical cancer tumorigenesis were further investigated. Knockdown of circTPCN significantly suppressed proliferation, migration, and invasion and increased apoptosis of cervical cancer cells in vitro. Molecular analysis revealed that circTPCN acted as a sponge of miR-634 to enhance mTOR expression. Thus, the circTPCN/miR-634/mTOR regulatory pathway might be involved in cervical cancer tumorigenesis, and circTPCN is a potential therapeutic target in cervical cancer.
•CircTPCN and circFAM185A were significantly up-regulated in in cervical cancer samples compared to normal cervix samples.•Knockdown of circTPCN suppressed proliferation, migration, invasion and increased the apoptosis of cervical cancer cells.•CircTPCN acted as a sponge of miR-634 to enhance mTOR expression.•CircTPCN/miR-634/mTOR regulatory pathway might be involved in cervical cancer tumorigenesis. |
| doi_str_mv | 10.1016/j.ygeno.2021.05.026 |
| format | Article |
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•CircTPCN and circFAM185A were significantly up-regulated in in cervical cancer samples compared to normal cervix samples.•Knockdown of circTPCN suppressed proliferation, migration, invasion and increased the apoptosis of cervical cancer cells.•CircTPCN acted as a sponge of miR-634 to enhance mTOR expression.•CircTPCN/miR-634/mTOR regulatory pathway might be involved in cervical cancer tumorigenesis.</description><identifier>ISSN: 0888-7543</identifier><identifier>EISSN: 1089-8646</identifier><identifier>DOI: 10.1016/j.ygeno.2021.05.026</identifier><identifier>PMID: 34029698</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>apoptosis ; biomarkers ; carcinogenesis ; Carcinogenesis - genetics ; Cell Proliferation - genetics ; Cervical cancer ; circTPCN ; Female ; gene expression regulation ; genomics ; Humans ; microarray technology ; MicroRNAs - genetics ; MicroRNAs - metabolism ; miR-634 ; mTOR ; non-coding RNA ; quantitative polymerase chain reaction ; RNA, Circular ; therapeutics ; TOR Serine-Threonine Kinases - genetics ; uterine cervical neoplasms ; Uterine Cervical Neoplasms - genetics</subject><ispartof>Genomics (San Diego, Calif.), 2021-07, Vol.113 (4), p.2253-2263</ispartof><rights>2021 Elsevier Inc.</rights><rights>Copyright © 2021 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c437t-b183e0f328e36d143ffcd55d255111b0c19ea09e946415a292f11c27541cbfc33</citedby><cites>FETCH-LOGICAL-c437t-b183e0f328e36d143ffcd55d255111b0c19ea09e946415a292f11c27541cbfc33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.ygeno.2021.05.026$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,45974</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34029698$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tian-zhao, Du</creatorcontrib><creatorcontrib>Yang, Yang</creatorcontrib><creatorcontrib>Xing-xuan, Wang</creatorcontrib><creatorcontrib>Yu-xin, Che</creatorcontrib><creatorcontrib>Xue-lian, Wang</creatorcontrib><title>Profiling of circular RNAs and circTPCN/miR-634/mTOR regulatory pathway in cervical cancer</title><title>Genomics (San Diego, Calif.)</title><addtitle>Genomics</addtitle><description>Circular RNAs (circRNAs) are highly stable forms of endogenous non-coding RNA molecules with diverse biological functions. Some of them have been demonstrated to play crucial roles in the initiation or development of cancers through regulation of gene expression. However, the profiles and the roles of circRNAs in tumorigenesis of cervical cancer remain largely unknown. In the current study, we investigated the expression profiles of circRNAs and their potential oncogenic mechanisms in cervical cancer. The expression patterns, obtained using a microarray assay, revealed a total of 192 differentially expressed circRNAs, of which 106 were upregulated and 86 were downregulated, in cervical cancer samples compared with normal cervical samples. The differential expression of circRNAs was validated using quantitative real-time polymerase chain reaction. Two circRNAs (circTPCN and circFAM185A) were confirmed to be significantly upregulated in cervical cancer samples, indicating that they represent potential biomarkers of cervical cancer. The role and the potential molecular mechanism of circTPCN in cervical cancer tumorigenesis were further investigated. Knockdown of circTPCN significantly suppressed proliferation, migration, and invasion and increased apoptosis of cervical cancer cells in vitro. Molecular analysis revealed that circTPCN acted as a sponge of miR-634 to enhance mTOR expression. Thus, the circTPCN/miR-634/mTOR regulatory pathway might be involved in cervical cancer tumorigenesis, and circTPCN is a potential therapeutic target in cervical cancer.
•CircTPCN and circFAM185A were significantly up-regulated in in cervical cancer samples compared to normal cervix samples.•Knockdown of circTPCN suppressed proliferation, migration, invasion and increased the apoptosis of cervical cancer cells.•CircTPCN acted as a sponge of miR-634 to enhance mTOR expression.•CircTPCN/miR-634/mTOR regulatory pathway might be involved in cervical cancer tumorigenesis.</description><subject>apoptosis</subject><subject>biomarkers</subject><subject>carcinogenesis</subject><subject>Carcinogenesis - genetics</subject><subject>Cell Proliferation - genetics</subject><subject>Cervical cancer</subject><subject>circTPCN</subject><subject>Female</subject><subject>gene expression regulation</subject><subject>genomics</subject><subject>Humans</subject><subject>microarray technology</subject><subject>MicroRNAs - genetics</subject><subject>MicroRNAs - metabolism</subject><subject>miR-634</subject><subject>mTOR</subject><subject>non-coding RNA</subject><subject>quantitative polymerase chain reaction</subject><subject>RNA, Circular</subject><subject>therapeutics</subject><subject>TOR Serine-Threonine Kinases - genetics</subject><subject>uterine cervical neoplasms</subject><subject>Uterine Cervical Neoplasms - genetics</subject><issn>0888-7543</issn><issn>1089-8646</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkE1PGzEQhi3UqqS0vwAJ-djLbsZfG--BA4pKWwkBitJLL5bjHQdH-xHsTar8ewyhPSJOMxo974zmIeScQcmAVdNNeVhjP5QcOCtBlcCrEzJhoOtCV7L6QCagtS5mSopT8jmlDQDUQvNP5FRI4HVV6wn5cx8HH9rQr-ngqQvR7Vob6eL2KlHbNy-T5f38dtqFRVEJOe2WdwsacZ2xcYgHurXjw197oKGnDuM-ONtSZ_vcfyEfvW0Tfn2tZ-T39ffl_Gdxc_fj1_zqpnBSzMZixbRA8IJrFFXDpPDeNUo1XCnG2Aocq9FCjbWsJFOW19wz5nh-i7mVd0KckW_Hvds4PO4wjaYLyWHb2h6HXTJ5EQcJM9DvQAXnCuRMZlQcUReHlCJ6s42hs_FgGJhn_2ZjXvybZ_8GlMn-c-ri9cBu1WHzP_NPeAYujwBmI_uA0SQXMOtqQkQ3mmYIbx54AjoslXs</recordid><startdate>202107</startdate><enddate>202107</enddate><creator>Tian-zhao, Du</creator><creator>Yang, Yang</creator><creator>Xing-xuan, Wang</creator><creator>Yu-xin, Che</creator><creator>Xue-lian, Wang</creator><general>Elsevier Inc</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope></search><sort><creationdate>202107</creationdate><title>Profiling of circular RNAs and circTPCN/miR-634/mTOR regulatory pathway in cervical cancer</title><author>Tian-zhao, Du ; Yang, Yang ; Xing-xuan, Wang ; Yu-xin, Che ; Xue-lian, Wang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c437t-b183e0f328e36d143ffcd55d255111b0c19ea09e946415a292f11c27541cbfc33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>apoptosis</topic><topic>biomarkers</topic><topic>carcinogenesis</topic><topic>Carcinogenesis - genetics</topic><topic>Cell Proliferation - genetics</topic><topic>Cervical cancer</topic><topic>circTPCN</topic><topic>Female</topic><topic>gene expression regulation</topic><topic>genomics</topic><topic>Humans</topic><topic>microarray technology</topic><topic>MicroRNAs - genetics</topic><topic>MicroRNAs - metabolism</topic><topic>miR-634</topic><topic>mTOR</topic><topic>non-coding RNA</topic><topic>quantitative polymerase chain reaction</topic><topic>RNA, Circular</topic><topic>therapeutics</topic><topic>TOR Serine-Threonine Kinases - genetics</topic><topic>uterine cervical neoplasms</topic><topic>Uterine Cervical Neoplasms - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tian-zhao, Du</creatorcontrib><creatorcontrib>Yang, Yang</creatorcontrib><creatorcontrib>Xing-xuan, Wang</creatorcontrib><creatorcontrib>Yu-xin, Che</creatorcontrib><creatorcontrib>Xue-lian, Wang</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>Genomics (San Diego, Calif.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tian-zhao, Du</au><au>Yang, Yang</au><au>Xing-xuan, Wang</au><au>Yu-xin, Che</au><au>Xue-lian, Wang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Profiling of circular RNAs and circTPCN/miR-634/mTOR regulatory pathway in cervical cancer</atitle><jtitle>Genomics (San Diego, Calif.)</jtitle><addtitle>Genomics</addtitle><date>2021-07</date><risdate>2021</risdate><volume>113</volume><issue>4</issue><spage>2253</spage><epage>2263</epage><pages>2253-2263</pages><issn>0888-7543</issn><eissn>1089-8646</eissn><abstract>Circular RNAs (circRNAs) are highly stable forms of endogenous non-coding RNA molecules with diverse biological functions. Some of them have been demonstrated to play crucial roles in the initiation or development of cancers through regulation of gene expression. However, the profiles and the roles of circRNAs in tumorigenesis of cervical cancer remain largely unknown. In the current study, we investigated the expression profiles of circRNAs and their potential oncogenic mechanisms in cervical cancer. The expression patterns, obtained using a microarray assay, revealed a total of 192 differentially expressed circRNAs, of which 106 were upregulated and 86 were downregulated, in cervical cancer samples compared with normal cervical samples. The differential expression of circRNAs was validated using quantitative real-time polymerase chain reaction. Two circRNAs (circTPCN and circFAM185A) were confirmed to be significantly upregulated in cervical cancer samples, indicating that they represent potential biomarkers of cervical cancer. The role and the potential molecular mechanism of circTPCN in cervical cancer tumorigenesis were further investigated. Knockdown of circTPCN significantly suppressed proliferation, migration, and invasion and increased apoptosis of cervical cancer cells in vitro. Molecular analysis revealed that circTPCN acted as a sponge of miR-634 to enhance mTOR expression. Thus, the circTPCN/miR-634/mTOR regulatory pathway might be involved in cervical cancer tumorigenesis, and circTPCN is a potential therapeutic target in cervical cancer.
•CircTPCN and circFAM185A were significantly up-regulated in in cervical cancer samples compared to normal cervix samples.•Knockdown of circTPCN suppressed proliferation, migration, invasion and increased the apoptosis of cervical cancer cells.•CircTPCN acted as a sponge of miR-634 to enhance mTOR expression.•CircTPCN/miR-634/mTOR regulatory pathway might be involved in cervical cancer tumorigenesis.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>34029698</pmid><doi>10.1016/j.ygeno.2021.05.026</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | apoptosis biomarkers carcinogenesis Carcinogenesis - genetics Cell Proliferation - genetics Cervical cancer circTPCN Female gene expression regulation genomics Humans microarray technology MicroRNAs - genetics MicroRNAs - metabolism miR-634 mTOR non-coding RNA quantitative polymerase chain reaction RNA, Circular therapeutics TOR Serine-Threonine Kinases - genetics uterine cervical neoplasms Uterine Cervical Neoplasms - genetics |
| title | Profiling of circular RNAs and circTPCN/miR-634/mTOR regulatory pathway in cervical cancer |
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