Antibacterial Effect and Bioactivity of Innovative and Currently Used Intracanal Medicaments in Regenerative Endodontics
The purpose of this study was to determine the antibacterial effect and bioactivity of triple antibiotic paste (TAP), calcium hydroxide (Ca[OH]2), and calcium hypochlorite (Ca[OCl]2). Root canals were infected with 3-week-old Enterococcus faecalis biofilm and then medicated for 7 days with TAP, Ca(O...
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| Veröffentlicht in: | Journal of endodontics 2021-08, Vol.47 (8), p.1294-1300 |
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| creator | Alfadda, Sarah Alquria, Theeb Karaismailoglu, Eda Aksel, Hacer Azim, Adham A. |
| description | The purpose of this study was to determine the antibacterial effect and bioactivity of triple antibiotic paste (TAP), calcium hydroxide (Ca[OH]2), and calcium hypochlorite (Ca[OCl]2).
Root canals were infected with 3-week-old Enterococcus faecalis biofilm and then medicated for 7 days with TAP, Ca(OH)2, or Ca(OCl)2 (n = 10/group). Untreated and uninfected canals were used as positive and negative controls. The antibacterial effect was determined using colony-forming units and a Live/Dead bacterial viability kit. Dental pulp stem cells were seeded on medicated dentin surfaces for 7 days. Sodium thiosulfate and various concentrations of ascorbic acid (1%, 5%, and 10%) were also used to neutralize the samples treated with Ca(OCl)2 before cell seeding (n = 3 in triplicate). Cell viability and morphology were evaluated using a viability assay and Live/Dead cell analysis. Alkaline phosphatase (ALP) activity was also measured to determine the cells’ mineralization activity.
All medicaments decreased the initial bacterial load (P |
| doi_str_mv | 10.1016/j.joen.2021.05.005 |
| format | Article |
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Root canals were infected with 3-week-old Enterococcus faecalis biofilm and then medicated for 7 days with TAP, Ca(OH)2, or Ca(OCl)2 (n = 10/group). Untreated and uninfected canals were used as positive and negative controls. The antibacterial effect was determined using colony-forming units and a Live/Dead bacterial viability kit. Dental pulp stem cells were seeded on medicated dentin surfaces for 7 days. Sodium thiosulfate and various concentrations of ascorbic acid (1%, 5%, and 10%) were also used to neutralize the samples treated with Ca(OCl)2 before cell seeding (n = 3 in triplicate). Cell viability and morphology were evaluated using a viability assay and Live/Dead cell analysis. Alkaline phosphatase (ALP) activity was also measured to determine the cells’ mineralization activity.
All medicaments decreased the initial bacterial load (P < .05). The highest bacterial reduction in the main canal and dentinal tubules was observed in the Ca(OCl)2 group (P < .05). TAP- or Ca(OH)2-treated dentin surface improved cell viability and ALP activity compared with the untreated dentin surface (P < .05), whereas Ca(OCl)2 decreased cell viability and ALP activity (P < .05). Ten percent ascorbic acid neutralized the effect of Ca(OCl)2 on the treated dentin surface, showing higher cell viability (P < .05) and similar ALP activity with the untreated dentin surface and the other groups (P > .05).
Ca(OCl)2 medication improved root canal disinfection against E. faecalis biofilm compared with TAP and Ca(OH)2. The adverse effects caused by Ca(OCl)2 on cell viability and mineralization activity can be neutralized with 10% ascorbic acid.</description><identifier>ISSN: 0099-2399</identifier><identifier>EISSN: 1878-3554</identifier><identifier>DOI: 10.1016/j.joen.2021.05.005</identifier><language>eng</language><publisher>Elsevier Inc</publisher><subject>Ascorbic acid ; calcium hypochlorite ; disinfection ; intracanal medicament ; neutralization ; regenerative endodontics</subject><ispartof>Journal of endodontics, 2021-08, Vol.47 (8), p.1294-1300</ispartof><rights>2021</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c333t-b7049c5e881a68ba86aa728bddc7ae42862bfc8abfa2f042e0725c724618fdfc3</citedby><cites>FETCH-LOGICAL-c333t-b7049c5e881a68ba86aa728bddc7ae42862bfc8abfa2f042e0725c724618fdfc3</cites><orcidid>0000-0002-4603-5650</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0099239921003666$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids></links><search><creatorcontrib>Alfadda, Sarah</creatorcontrib><creatorcontrib>Alquria, Theeb</creatorcontrib><creatorcontrib>Karaismailoglu, Eda</creatorcontrib><creatorcontrib>Aksel, Hacer</creatorcontrib><creatorcontrib>Azim, Adham A.</creatorcontrib><title>Antibacterial Effect and Bioactivity of Innovative and Currently Used Intracanal Medicaments in Regenerative Endodontics</title><title>Journal of endodontics</title><description>The purpose of this study was to determine the antibacterial effect and bioactivity of triple antibiotic paste (TAP), calcium hydroxide (Ca[OH]2), and calcium hypochlorite (Ca[OCl]2).
Root canals were infected with 3-week-old Enterococcus faecalis biofilm and then medicated for 7 days with TAP, Ca(OH)2, or Ca(OCl)2 (n = 10/group). Untreated and uninfected canals were used as positive and negative controls. The antibacterial effect was determined using colony-forming units and a Live/Dead bacterial viability kit. Dental pulp stem cells were seeded on medicated dentin surfaces for 7 days. Sodium thiosulfate and various concentrations of ascorbic acid (1%, 5%, and 10%) were also used to neutralize the samples treated with Ca(OCl)2 before cell seeding (n = 3 in triplicate). Cell viability and morphology were evaluated using a viability assay and Live/Dead cell analysis. Alkaline phosphatase (ALP) activity was also measured to determine the cells’ mineralization activity.
All medicaments decreased the initial bacterial load (P < .05). The highest bacterial reduction in the main canal and dentinal tubules was observed in the Ca(OCl)2 group (P < .05). TAP- or Ca(OH)2-treated dentin surface improved cell viability and ALP activity compared with the untreated dentin surface (P < .05), whereas Ca(OCl)2 decreased cell viability and ALP activity (P < .05). Ten percent ascorbic acid neutralized the effect of Ca(OCl)2 on the treated dentin surface, showing higher cell viability (P < .05) and similar ALP activity with the untreated dentin surface and the other groups (P > .05).
Ca(OCl)2 medication improved root canal disinfection against E. faecalis biofilm compared with TAP and Ca(OH)2. The adverse effects caused by Ca(OCl)2 on cell viability and mineralization activity can be neutralized with 10% ascorbic acid.</description><subject>Ascorbic acid</subject><subject>calcium hypochlorite</subject><subject>disinfection</subject><subject>intracanal medicament</subject><subject>neutralization</subject><subject>regenerative endodontics</subject><issn>0099-2399</issn><issn>1878-3554</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNp9kE9rGzEQxUVJoU7aL9DTHnPZ7UjaP1rIJTVOGkgIlOYsZqVRkVlLibQ29bevHPfc0zDz3pthfox95dBw4P23bbONFBoBgjfQNQDdB7bialC17Lr2gq0AxrEWchw_scuctwB8kHJYsT-3YfETmoWSx7naOEdmqTDY6ruPZewPfjlW0VUPIcQDlp7e1fU-JQrLfKxeMtmiLgkNhrLiiaw3uCtirnyoftJvCpTOyU2w0cZy0eTP7KPDOdOXf_WKvdxtfq1_1I_P9w_r28faSCmXehqgHU1HSnHs1YSqRxyEmqw1A1IrVC8mZxRODoWDVhAMojODaHuunHVGXrHr897XFN_2lBe989nQPGOguM9adFKIlrcAxSrOVpNizomcfk1-h-moOegTZr3VJ8z6hFlDpwvmEro5h6g8cfCUdDaegikUUkGpbfT_i_8Fe9SJBw</recordid><startdate>202108</startdate><enddate>202108</enddate><creator>Alfadda, Sarah</creator><creator>Alquria, Theeb</creator><creator>Karaismailoglu, Eda</creator><creator>Aksel, Hacer</creator><creator>Azim, Adham A.</creator><general>Elsevier Inc</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-4603-5650</orcidid></search><sort><creationdate>202108</creationdate><title>Antibacterial Effect and Bioactivity of Innovative and Currently Used Intracanal Medicaments in Regenerative Endodontics</title><author>Alfadda, Sarah ; Alquria, Theeb ; Karaismailoglu, Eda ; Aksel, Hacer ; Azim, Adham A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c333t-b7049c5e881a68ba86aa728bddc7ae42862bfc8abfa2f042e0725c724618fdfc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Ascorbic acid</topic><topic>calcium hypochlorite</topic><topic>disinfection</topic><topic>intracanal medicament</topic><topic>neutralization</topic><topic>regenerative endodontics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Alfadda, Sarah</creatorcontrib><creatorcontrib>Alquria, Theeb</creatorcontrib><creatorcontrib>Karaismailoglu, Eda</creatorcontrib><creatorcontrib>Aksel, Hacer</creatorcontrib><creatorcontrib>Azim, Adham A.</creatorcontrib><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of endodontics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Alfadda, Sarah</au><au>Alquria, Theeb</au><au>Karaismailoglu, Eda</au><au>Aksel, Hacer</au><au>Azim, Adham A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Antibacterial Effect and Bioactivity of Innovative and Currently Used Intracanal Medicaments in Regenerative Endodontics</atitle><jtitle>Journal of endodontics</jtitle><date>2021-08</date><risdate>2021</risdate><volume>47</volume><issue>8</issue><spage>1294</spage><epage>1300</epage><pages>1294-1300</pages><issn>0099-2399</issn><eissn>1878-3554</eissn><abstract>The purpose of this study was to determine the antibacterial effect and bioactivity of triple antibiotic paste (TAP), calcium hydroxide (Ca[OH]2), and calcium hypochlorite (Ca[OCl]2).
Root canals were infected with 3-week-old Enterococcus faecalis biofilm and then medicated for 7 days with TAP, Ca(OH)2, or Ca(OCl)2 (n = 10/group). Untreated and uninfected canals were used as positive and negative controls. The antibacterial effect was determined using colony-forming units and a Live/Dead bacterial viability kit. Dental pulp stem cells were seeded on medicated dentin surfaces for 7 days. Sodium thiosulfate and various concentrations of ascorbic acid (1%, 5%, and 10%) were also used to neutralize the samples treated with Ca(OCl)2 before cell seeding (n = 3 in triplicate). Cell viability and morphology were evaluated using a viability assay and Live/Dead cell analysis. Alkaline phosphatase (ALP) activity was also measured to determine the cells’ mineralization activity.
All medicaments decreased the initial bacterial load (P < .05). The highest bacterial reduction in the main canal and dentinal tubules was observed in the Ca(OCl)2 group (P < .05). TAP- or Ca(OH)2-treated dentin surface improved cell viability and ALP activity compared with the untreated dentin surface (P < .05), whereas Ca(OCl)2 decreased cell viability and ALP activity (P < .05). Ten percent ascorbic acid neutralized the effect of Ca(OCl)2 on the treated dentin surface, showing higher cell viability (P < .05) and similar ALP activity with the untreated dentin surface and the other groups (P > .05).
Ca(OCl)2 medication improved root canal disinfection against E. faecalis biofilm compared with TAP and Ca(OH)2. The adverse effects caused by Ca(OCl)2 on cell viability and mineralization activity can be neutralized with 10% ascorbic acid.</abstract><pub>Elsevier Inc</pub><doi>10.1016/j.joen.2021.05.005</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0002-4603-5650</orcidid></addata></record> |
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| subjects | Ascorbic acid calcium hypochlorite disinfection intracanal medicament neutralization regenerative endodontics |
| title | Antibacterial Effect and Bioactivity of Innovative and Currently Used Intracanal Medicaments in Regenerative Endodontics |
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