Metabolite fingerprinting, in vitro antimicrobial and antioxidant activities and in-silico docking in Alloteropsis cimicina and its endophytic fungus Penicillium pinophilum
Endophytic fungal interaction with medicinal plant resulting in the production of bioactive compounds influenced the present study. Endophytic fungus Penicillium pinophilum Hedgc. from Alloteropsis cimicina with high incidence was isolated by incubation methods and characterized by morphological and...
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| Veröffentlicht in: | Molecular biology reports 2021-05, Vol.48 (5), p.4021-4037 |
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| description | Endophytic fungal interaction with medicinal plant resulting in the production of bioactive compounds influenced the present study. Endophytic fungus
Penicillium pinophilum
Hedgc. from
Alloteropsis cimicina
with high incidence was isolated by incubation methods and characterized by morphological and molecular (ITS rDNA region) methods.
Penicillium pinophilum
was cultured on PD broth and metabolites of host and endophyte were extracted with ethyl acetate and methanol. Metabolites were assayed for antimicrobial potential by well diffusion and scavenging ability by spectrophotometric and electrochemical methods. Metabolite profiling by Orbitrap High-Resolution Liquid Chromatography-Mass Spectrometry and Fourier-transform infrared spectroscopy and by validation of antimicrobial and antioxidant activities in-silico drug-likeness of spectral compound prediction and molecular docking were performed. Host and
P. pinophilum
extracts showed strong antimicrobial potential against certain clinical bacterial strains and
Fusarium oxysporum.
Fungal extracts exhibited higher antioxidant activity than
A. cimicina
extract. Metabolite profiling indicated 14 and 21 antimicrobial, 10 and 13 antioxidant compounds in
A. cimicina
and
P. pinophilum
extracts, respectively. There were eight spectral compounds common to endophyte and host with high binding affinity towards receptors. The present study revealed that
P. pinophilum
and
A. cimicina
are natural reservoirs of novel bioactive compounds with antimicrobial and antioxidant properties.
Graphic Abstract |
| doi_str_mv | 10.1007/s11033-021-06410-0 |
| format | Article |
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Penicillium pinophilum
Hedgc. from
Alloteropsis cimicina
with high incidence was isolated by incubation methods and characterized by morphological and molecular (ITS rDNA region) methods.
Penicillium pinophilum
was cultured on PD broth and metabolites of host and endophyte were extracted with ethyl acetate and methanol. Metabolites were assayed for antimicrobial potential by well diffusion and scavenging ability by spectrophotometric and electrochemical methods. Metabolite profiling by Orbitrap High-Resolution Liquid Chromatography-Mass Spectrometry and Fourier-transform infrared spectroscopy and by validation of antimicrobial and antioxidant activities in-silico drug-likeness of spectral compound prediction and molecular docking were performed. Host and
P. pinophilum
extracts showed strong antimicrobial potential against certain clinical bacterial strains and
Fusarium oxysporum.
Fungal extracts exhibited higher antioxidant activity than
A. cimicina
extract. Metabolite profiling indicated 14 and 21 antimicrobial, 10 and 13 antioxidant compounds in
A. cimicina
and
P. pinophilum
extracts, respectively. There were eight spectral compounds common to endophyte and host with high binding affinity towards receptors. The present study revealed that
P. pinophilum
and
A. cimicina
are natural reservoirs of novel bioactive compounds with antimicrobial and antioxidant properties.
Graphic Abstract</description><identifier>ISSN: 0301-4851</identifier><identifier>EISSN: 1573-4978</identifier><identifier>DOI: 10.1007/s11033-021-06410-0</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Acetic acid ; Alloteropsis cimicina ; Animal Anatomy ; Animal Biochemistry ; Antioxidants ; Bioactive compounds ; Biomedical and Life Sciences ; Endophytes ; Ethyl acetate ; Fingerprinting ; Histology ; Infrared spectroscopy ; Life Sciences ; Liquid chromatography ; Mass spectroscopy ; Medicinal plants ; Metabolites ; Morphology ; Original Article ; Penicillium ; Spectrophotometry</subject><ispartof>Molecular biology reports, 2021-05, Vol.48 (5), p.4021-4037</ispartof><rights>The Author(s), under exclusive licence to Springer Nature B.V. 2021</rights><rights>The Author(s), under exclusive licence to Springer Nature B.V. 2021.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c352t-de9a5ea38bf531062c3bb1b7fb60a45895b3368ff689787811fd658b54dc5ee83</citedby><cites>FETCH-LOGICAL-c352t-de9a5ea38bf531062c3bb1b7fb60a45895b3368ff689787811fd658b54dc5ee83</cites><orcidid>0000-0001-5308-2917</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11033-021-06410-0$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11033-021-06410-0$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids></links><search><creatorcontrib>Nischitha, R.</creatorcontrib><creatorcontrib>Shivanna, M. B.</creatorcontrib><title>Metabolite fingerprinting, in vitro antimicrobial and antioxidant activities and in-silico docking in Alloteropsis cimicina and its endophytic fungus Penicillium pinophilum</title><title>Molecular biology reports</title><addtitle>Mol Biol Rep</addtitle><description>Endophytic fungal interaction with medicinal plant resulting in the production of bioactive compounds influenced the present study. Endophytic fungus
Penicillium pinophilum
Hedgc. from
Alloteropsis cimicina
with high incidence was isolated by incubation methods and characterized by morphological and molecular (ITS rDNA region) methods.
Penicillium pinophilum
was cultured on PD broth and metabolites of host and endophyte were extracted with ethyl acetate and methanol. Metabolites were assayed for antimicrobial potential by well diffusion and scavenging ability by spectrophotometric and electrochemical methods. Metabolite profiling by Orbitrap High-Resolution Liquid Chromatography-Mass Spectrometry and Fourier-transform infrared spectroscopy and by validation of antimicrobial and antioxidant activities in-silico drug-likeness of spectral compound prediction and molecular docking were performed. Host and
P. pinophilum
extracts showed strong antimicrobial potential against certain clinical bacterial strains and
Fusarium oxysporum.
Fungal extracts exhibited higher antioxidant activity than
A. cimicina
extract. Metabolite profiling indicated 14 and 21 antimicrobial, 10 and 13 antioxidant compounds in
A. cimicina
and
P. pinophilum
extracts, respectively. There were eight spectral compounds common to endophyte and host with high binding affinity towards receptors. The present study revealed that
P. pinophilum
and
A. cimicina
are natural reservoirs of novel bioactive compounds with antimicrobial and antioxidant properties.
Graphic Abstract</description><subject>Acetic acid</subject><subject>Alloteropsis cimicina</subject><subject>Animal Anatomy</subject><subject>Animal Biochemistry</subject><subject>Antioxidants</subject><subject>Bioactive compounds</subject><subject>Biomedical and Life Sciences</subject><subject>Endophytes</subject><subject>Ethyl acetate</subject><subject>Fingerprinting</subject><subject>Histology</subject><subject>Infrared spectroscopy</subject><subject>Life Sciences</subject><subject>Liquid chromatography</subject><subject>Mass spectroscopy</subject><subject>Medicinal plants</subject><subject>Metabolites</subject><subject>Morphology</subject><subject>Original Article</subject><subject>Penicillium</subject><subject>Spectrophotometry</subject><issn>0301-4851</issn><issn>1573-4978</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kc9uFSEUh4mxidfqC7gicePCURhg4C6bxn9JTV3UNQEG6qkMjANj2nfyIWXumJi4cAWc850v5PwQekHJG0qIfFsoJYx1pKcdGTglHXmEDlRI1vGjVI_RgTBCO64EfYKelnJHCOFUigP69dlXY3OE6nGAdOuXeYFU2-01hoR_Ql0yNq0wgVuyBRPbazxV8j2M7cTGVWgc-HJqQeoKRHAZj9l9b6LNcxFjrn7Jc4GC3SaDZHa8FuzTmOdvDxUcDmu6XQv-4lNDYoR1wjOk1oW4Ts_QWTCx-Od_znP09f27m8uP3dX1h0-XF1edY6Kv3eiPRnjDlA2CUTL0jllLrQx2IIYLdRSWsUGFMKi2HKkoDeMglBV8dMJ7xc7Rq907L_nH6kvVExTnYzTJ57XovmkF75UkDX35D3qX1yW13zVKSsk5Y7xR_U61HZay-KDbliezPGhK9Bag3gPULUB9ClBvarYPlS2Slsxf9X-mfgPYSaKJ</recordid><startdate>20210501</startdate><enddate>20210501</enddate><creator>Nischitha, R.</creator><creator>Shivanna, M. B.</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TK</scope><scope>7TM</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-5308-2917</orcidid></search><sort><creationdate>20210501</creationdate><title>Metabolite fingerprinting, in vitro antimicrobial and antioxidant activities and in-silico docking in Alloteropsis cimicina and its endophytic fungus Penicillium pinophilum</title><author>Nischitha, R. ; Shivanna, M. B.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c352t-de9a5ea38bf531062c3bb1b7fb60a45895b3368ff689787811fd658b54dc5ee83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Acetic acid</topic><topic>Alloteropsis cimicina</topic><topic>Animal Anatomy</topic><topic>Animal Biochemistry</topic><topic>Antioxidants</topic><topic>Bioactive compounds</topic><topic>Biomedical and Life Sciences</topic><topic>Endophytes</topic><topic>Ethyl acetate</topic><topic>Fingerprinting</topic><topic>Histology</topic><topic>Infrared spectroscopy</topic><topic>Life Sciences</topic><topic>Liquid chromatography</topic><topic>Mass spectroscopy</topic><topic>Medicinal plants</topic><topic>Metabolites</topic><topic>Morphology</topic><topic>Original Article</topic><topic>Penicillium</topic><topic>Spectrophotometry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nischitha, R.</creatorcontrib><creatorcontrib>Shivanna, M. 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B.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Metabolite fingerprinting, in vitro antimicrobial and antioxidant activities and in-silico docking in Alloteropsis cimicina and its endophytic fungus Penicillium pinophilum</atitle><jtitle>Molecular biology reports</jtitle><stitle>Mol Biol Rep</stitle><date>2021-05-01</date><risdate>2021</risdate><volume>48</volume><issue>5</issue><spage>4021</spage><epage>4037</epage><pages>4021-4037</pages><issn>0301-4851</issn><eissn>1573-4978</eissn><abstract>Endophytic fungal interaction with medicinal plant resulting in the production of bioactive compounds influenced the present study. Endophytic fungus
Penicillium pinophilum
Hedgc. from
Alloteropsis cimicina
with high incidence was isolated by incubation methods and characterized by morphological and molecular (ITS rDNA region) methods.
Penicillium pinophilum
was cultured on PD broth and metabolites of host and endophyte were extracted with ethyl acetate and methanol. Metabolites were assayed for antimicrobial potential by well diffusion and scavenging ability by spectrophotometric and electrochemical methods. Metabolite profiling by Orbitrap High-Resolution Liquid Chromatography-Mass Spectrometry and Fourier-transform infrared spectroscopy and by validation of antimicrobial and antioxidant activities in-silico drug-likeness of spectral compound prediction and molecular docking were performed. Host and
P. pinophilum
extracts showed strong antimicrobial potential against certain clinical bacterial strains and
Fusarium oxysporum.
Fungal extracts exhibited higher antioxidant activity than
A. cimicina
extract. Metabolite profiling indicated 14 and 21 antimicrobial, 10 and 13 antioxidant compounds in
A. cimicina
and
P. pinophilum
extracts, respectively. There were eight spectral compounds common to endophyte and host with high binding affinity towards receptors. The present study revealed that
P. pinophilum
and
A. cimicina
are natural reservoirs of novel bioactive compounds with antimicrobial and antioxidant properties.
Graphic Abstract</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><doi>10.1007/s11033-021-06410-0</doi><tpages>17</tpages><orcidid>https://orcid.org/0000-0001-5308-2917</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0301-4851 |
| ispartof | Molecular biology reports, 2021-05, Vol.48 (5), p.4021-4037 |
| issn | 0301-4851 1573-4978 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2531542870 |
| source | SpringerLink Journals - AutoHoldings |
| subjects | Acetic acid Alloteropsis cimicina Animal Anatomy Animal Biochemistry Antioxidants Bioactive compounds Biomedical and Life Sciences Endophytes Ethyl acetate Fingerprinting Histology Infrared spectroscopy Life Sciences Liquid chromatography Mass spectroscopy Medicinal plants Metabolites Morphology Original Article Penicillium Spectrophotometry |
| title | Metabolite fingerprinting, in vitro antimicrobial and antioxidant activities and in-silico docking in Alloteropsis cimicina and its endophytic fungus Penicillium pinophilum |
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