Identification of reliable reference genes for expression studies in the magnum of laying hens housed in cage and cage‐free systems
Stress factors during poultry production can evoke changes in gene transcription and protein synthesis in the hen oviduct and could affect the internal and external egg quality. Studies of relative gene expression require the identification of the most stable reference genes for the quantitative pol...
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| creator | Rodríguez‐Hernández, Roy Oviedo‐Rondón, Edgar O. Rondón‐Barragán, Iang S. |
| description | Stress factors during poultry production can evoke changes in gene transcription and protein synthesis in the hen oviduct and could affect the internal and external egg quality. Studies of relative gene expression require the identification of the most stable reference genes for the quantitative polymerase chain reaction (qPCR) to investigate the reproductive tissues' response in laying hens kept in different production systems. The objective of this study was to determine the most stable reference genes of the magnum tissues of laying hens housed in two different production systems. Hy‐Line Brown hens were reared under the same sanitary conditions until 15 weeks of age. Later on, they were transferred into two different production systems, conventional cage (CC) and cage free (CF), up to 82 weeks of age. At 50 and 60 weeks, a total of six hens from each production system were euthanized, and magnum samples were collected. The qPCR was used to determine the RNA transcription level of five reference genes, ACTB, 18S, GAPDH, MSX2 and HMBS. These genes were evaluated for transcript expression in magnum tissues by NormFinder, BestKeeper, geNorm and RefFinder software. The results indicated that the most stable gene in the CF housing system was HMBS in three of the algorithms and in the CC housing system was the 18S, and the best combination of reference genes was HMBS/GAPDH in CF and 18S/HMBS in CC. In conclusion, HMBS, 18S and GAPDH genes could be used together as reference genes for the normalization of the magnum tissues transcript expression of hens in CF and CC housing systems.
Stressor factors during poultry production can produce changes in gene transcription and protein synthesis on the hen oviduct. Studies of relative gene expression require the identification of the most stable reference genes. The aim of this study was to determine the most stable reference genes of the magnum tissues of laying hens housed in two different production systems for welfare studies. |
| doi_str_mv | 10.1002/vms3.507 |
| format | Article |
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Stressor factors during poultry production can produce changes in gene transcription and protein synthesis on the hen oviduct. Studies of relative gene expression require the identification of the most stable reference genes. The aim of this study was to determine the most stable reference genes of the magnum tissues of laying hens housed in two different production systems for welfare studies.</description><identifier>ISSN: 2053-1095</identifier><identifier>EISSN: 2053-1095</identifier><identifier>DOI: 10.1002/vms3.507</identifier><identifier>PMID: 34015187</identifier><language>eng</language><publisher>England: John Wiley & Sons, Inc</publisher><subject>Animals ; Chickens - genetics ; Dehydrogenases ; Female ; Gene expression ; Glyceraldehyde-3-phosphate dehydrogenase ; Housing conditions ; Housing, Animal ; Laboratory animals ; magnum ; Msx2 protein ; normalization ; Original ; Oviduct ; Polymerase chain reaction ; Poultry ; Protein biosynthesis ; qPCR ; Software ; Thermal cycling ; Transcription ; transcriptomic ; welfare</subject><ispartof>Veterinary medicine and science, 2021-09, Vol.7 (5), p.1890-1898</ispartof><rights>2021 The Authors Published by John Wiley & Sons Ltd</rights><rights>2021 The Authors Veterinary Medicine and Science Published by John Wiley & Sons Ltd.</rights><rights>2021. This work is published under http://creativecommons.org/licenses/by-nc/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5047-e44709ad7d14c6f5510bca28d59f8fe049769df36fccb7b4ec47007b7be78d93</citedby><cites>FETCH-LOGICAL-c5047-e44709ad7d14c6f5510bca28d59f8fe049769df36fccb7b4ec47007b7be78d93</cites><orcidid>0000-0002-4187-7381 ; 0000-0002-1532-8282 ; 0000-0001-6980-892X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8464264/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8464264/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,1417,2102,11562,27924,27925,45574,45575,46052,46476,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34015187$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rodríguez‐Hernández, Roy</creatorcontrib><creatorcontrib>Oviedo‐Rondón, Edgar O.</creatorcontrib><creatorcontrib>Rondón‐Barragán, Iang S.</creatorcontrib><title>Identification of reliable reference genes for expression studies in the magnum of laying hens housed in cage and cage‐free systems</title><title>Veterinary medicine and science</title><addtitle>Vet Med Sci</addtitle><description>Stress factors during poultry production can evoke changes in gene transcription and protein synthesis in the hen oviduct and could affect the internal and external egg quality. Studies of relative gene expression require the identification of the most stable reference genes for the quantitative polymerase chain reaction (qPCR) to investigate the reproductive tissues' response in laying hens kept in different production systems. The objective of this study was to determine the most stable reference genes of the magnum tissues of laying hens housed in two different production systems. Hy‐Line Brown hens were reared under the same sanitary conditions until 15 weeks of age. Later on, they were transferred into two different production systems, conventional cage (CC) and cage free (CF), up to 82 weeks of age. At 50 and 60 weeks, a total of six hens from each production system were euthanized, and magnum samples were collected. The qPCR was used to determine the RNA transcription level of five reference genes, ACTB, 18S, GAPDH, MSX2 and HMBS. These genes were evaluated for transcript expression in magnum tissues by NormFinder, BestKeeper, geNorm and RefFinder software. The results indicated that the most stable gene in the CF housing system was HMBS in three of the algorithms and in the CC housing system was the 18S, and the best combination of reference genes was HMBS/GAPDH in CF and 18S/HMBS in CC. In conclusion, HMBS, 18S and GAPDH genes could be used together as reference genes for the normalization of the magnum tissues transcript expression of hens in CF and CC housing systems.
Stressor factors during poultry production can produce changes in gene transcription and protein synthesis on the hen oviduct. Studies of relative gene expression require the identification of the most stable reference genes. The aim of this study was to determine the most stable reference genes of the magnum tissues of laying hens housed in two different production systems for welfare studies.</description><subject>Animals</subject><subject>Chickens - genetics</subject><subject>Dehydrogenases</subject><subject>Female</subject><subject>Gene expression</subject><subject>Glyceraldehyde-3-phosphate dehydrogenase</subject><subject>Housing conditions</subject><subject>Housing, Animal</subject><subject>Laboratory animals</subject><subject>magnum</subject><subject>Msx2 protein</subject><subject>normalization</subject><subject>Original</subject><subject>Oviduct</subject><subject>Polymerase chain reaction</subject><subject>Poultry</subject><subject>Protein biosynthesis</subject><subject>qPCR</subject><subject>Software</subject><subject>Thermal cycling</subject><subject>Transcription</subject><subject>transcriptomic</subject><subject>welfare</subject><issn>2053-1095</issn><issn>2053-1095</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>24P</sourceid><sourceid>WIN</sourceid><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNp1ks1u1DAQgCMEolWpxBMgS1y4pDj-iZMLEqqgrFTEgYqr5djjrFeJvdhJy9649M4z8iQ43VJtkTh5PP70eTQzRfGywmcVxuTt9ZjoGcfiSXFMMKdlhVv-9CA-Kk5T2mCMK84E5eJ5cURZvlSNOC5uVwb85KzTanLBo2BRhMGpboAcWIjgNaAePCRkQ0TwYxshpQVN02xcTjuPpjWgUfV-HhfBoHbO92gNPqF1mBOYhdGqB6S8uQt-__xlIwBKuzTBmF4Uz6waEpzenyfF1ccPV-efyssvF6vz95el5piJEhgTuFVGmIrp2nJe4U4r0hje2sYCZq2oW2NpbbXuRMdAZx6LHIJoTEtPitVea4LayG10o4o7GZSTd4kQe6ni5PQAEoMVhHSNyL8x04CirSKEKMWhbjTw7Hq3d23nbgSjcxejGh5JH794t5Z9uJYNqxmpWRa8uRfE8H2GNMnRJQ3DoDzkpknCaUUIo22V0df_oJswR587lSlR52lyfiDUMaSUh_dQTIXlsily2RSZNyWjrw6LfwD_7kUGyj1w4wbY_Vckv33-ShfhHzTwywI</recordid><startdate>202109</startdate><enddate>202109</enddate><creator>Rodríguez‐Hernández, Roy</creator><creator>Oviedo‐Rondón, Edgar O.</creator><creator>Rondón‐Barragán, Iang S.</creator><general>John Wiley & Sons, Inc</general><general>John Wiley and Sons Inc</general><general>Wiley</general><scope>24P</scope><scope>WIN</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FE</scope><scope>8FH</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0002-4187-7381</orcidid><orcidid>https://orcid.org/0000-0002-1532-8282</orcidid><orcidid>https://orcid.org/0000-0001-6980-892X</orcidid></search><sort><creationdate>202109</creationdate><title>Identification of reliable reference genes for expression studies in the magnum of laying hens housed in cage and cage‐free systems</title><author>Rodríguez‐Hernández, Roy ; Oviedo‐Rondón, Edgar O. ; Rondón‐Barragán, Iang S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5047-e44709ad7d14c6f5510bca28d59f8fe049769df36fccb7b4ec47007b7be78d93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Animals</topic><topic>Chickens - genetics</topic><topic>Dehydrogenases</topic><topic>Female</topic><topic>Gene expression</topic><topic>Glyceraldehyde-3-phosphate dehydrogenase</topic><topic>Housing conditions</topic><topic>Housing, Animal</topic><topic>Laboratory animals</topic><topic>magnum</topic><topic>Msx2 protein</topic><topic>normalization</topic><topic>Original</topic><topic>Oviduct</topic><topic>Polymerase chain reaction</topic><topic>Poultry</topic><topic>Protein biosynthesis</topic><topic>qPCR</topic><topic>Software</topic><topic>Thermal cycling</topic><topic>Transcription</topic><topic>transcriptomic</topic><topic>welfare</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rodríguez‐Hernández, Roy</creatorcontrib><creatorcontrib>Oviedo‐Rondón, Edgar O.</creatorcontrib><creatorcontrib>Rondón‐Barragán, Iang S.</creatorcontrib><collection>Wiley-Blackwell Open Access Titles</collection><collection>Wiley Free Content</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection (ProQuest)</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Biological Science Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Veterinary medicine and science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rodríguez‐Hernández, Roy</au><au>Oviedo‐Rondón, Edgar O.</au><au>Rondón‐Barragán, Iang S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of reliable reference genes for expression studies in the magnum of laying hens housed in cage and cage‐free systems</atitle><jtitle>Veterinary medicine and science</jtitle><addtitle>Vet Med Sci</addtitle><date>2021-09</date><risdate>2021</risdate><volume>7</volume><issue>5</issue><spage>1890</spage><epage>1898</epage><pages>1890-1898</pages><issn>2053-1095</issn><eissn>2053-1095</eissn><abstract>Stress factors during poultry production can evoke changes in gene transcription and protein synthesis in the hen oviduct and could affect the internal and external egg quality. Studies of relative gene expression require the identification of the most stable reference genes for the quantitative polymerase chain reaction (qPCR) to investigate the reproductive tissues' response in laying hens kept in different production systems. The objective of this study was to determine the most stable reference genes of the magnum tissues of laying hens housed in two different production systems. Hy‐Line Brown hens were reared under the same sanitary conditions until 15 weeks of age. Later on, they were transferred into two different production systems, conventional cage (CC) and cage free (CF), up to 82 weeks of age. At 50 and 60 weeks, a total of six hens from each production system were euthanized, and magnum samples were collected. The qPCR was used to determine the RNA transcription level of five reference genes, ACTB, 18S, GAPDH, MSX2 and HMBS. These genes were evaluated for transcript expression in magnum tissues by NormFinder, BestKeeper, geNorm and RefFinder software. The results indicated that the most stable gene in the CF housing system was HMBS in three of the algorithms and in the CC housing system was the 18S, and the best combination of reference genes was HMBS/GAPDH in CF and 18S/HMBS in CC. In conclusion, HMBS, 18S and GAPDH genes could be used together as reference genes for the normalization of the magnum tissues transcript expression of hens in CF and CC housing systems.
Stressor factors during poultry production can produce changes in gene transcription and protein synthesis on the hen oviduct. Studies of relative gene expression require the identification of the most stable reference genes. The aim of this study was to determine the most stable reference genes of the magnum tissues of laying hens housed in two different production systems for welfare studies.</abstract><cop>England</cop><pub>John Wiley & Sons, Inc</pub><pmid>34015187</pmid><doi>10.1002/vms3.507</doi><tpages>0</tpages><orcidid>https://orcid.org/0000-0002-4187-7381</orcidid><orcidid>https://orcid.org/0000-0002-1532-8282</orcidid><orcidid>https://orcid.org/0000-0001-6980-892X</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Chickens - genetics Dehydrogenases Female Gene expression Glyceraldehyde-3-phosphate dehydrogenase Housing conditions Housing, Animal Laboratory animals magnum Msx2 protein normalization Original Oviduct Polymerase chain reaction Poultry Protein biosynthesis qPCR Software Thermal cycling Transcription transcriptomic welfare |
| title | Identification of reliable reference genes for expression studies in the magnum of laying hens housed in cage and cage‐free systems |
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