FASCIN regulates actin assembly for spindle movement and polar body extrusion in mouse oocyte meiosis
During mouse oocyte meiotic maturation, actin filaments play multiple roles in meiosis such as spindle migration and cytokinesis. FASCIN is shown to be an actin‐binding and bundling protein, making actin filaments tightly packed and parallel‐aligned, and FASCIN is involved in several cellular proces...
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| Veröffentlicht in: | Journal of cellular physiology 2021-11, Vol.236 (11), p.7725-7733 |
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| creator | Hu, Lin‐Lin Pan, Meng‐Hao Yang, Feng‐Lian Zong, Zi‐Ao Tang, Feng Pan, Zhen‐Nan Lu, Xiang Ren, Yan‐Ping Wang, Jun‐Li Sun, Shao‐Chen |
| description | During mouse oocyte meiotic maturation, actin filaments play multiple roles in meiosis such as spindle migration and cytokinesis. FASCIN is shown to be an actin‐binding and bundling protein, making actin filaments tightly packed and parallel‐aligned, and FASCIN is involved in several cellular processes like adhesion and migration. FASCIN is also a potential prognostic biomarker and therapeutic target for the treatment of metastatic disease. However, little is known about the functions of FASCIN in oocyte meiosis. In the present study, we knocked down the expression of FASCIN, and our results showed that FASCIN was essential for oocyte maturation. FASCIN was all expressed in the different stages of oocyte meiosis, and it mainly localized at the cortex of oocytes from the GV stage to the MII stage and showed a similar localization pattern with actin and DAAM1. Depletion of FASCIN affected the extrusion of the first polar body, and we also observed that some oocytes extruded from the large polar bodies. This might have resulted from the defects of actin assembly, which further affected the meiotic spindle positioning. In addition, we showed that inhibition of PKC activity decreased FASCIN expression, indicating that FASCIN might be regulated by PKC. Taken together, our results provided evidence for the important role of FASCIN on actin filaments for spindle migration and polar body extrusion in mouse oocyte meiosis.
Our results provided evidence for the important roles of FASCIN on actin filaments for spindle migration and polar body extrusion in mouse oocyte meiosis. |
| doi_str_mv | 10.1002/jcp.30443 |
| format | Article |
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Our results provided evidence for the important roles of FASCIN on actin filaments for spindle migration and polar body extrusion in mouse oocyte meiosis.</description><identifier>ISSN: 0021-9541</identifier><identifier>EISSN: 1097-4652</identifier><identifier>DOI: 10.1002/jcp.30443</identifier><identifier>PMID: 34018605</identifier><language>eng</language><publisher>United States: Wiley Subscription Services, Inc</publisher><subject>Actin ; Actin Cytoskeleton - genetics ; Actin Cytoskeleton - metabolism ; Animals ; Assembly ; Biomarkers ; Carrier Proteins - genetics ; Carrier Proteins - metabolism ; Cells, Cultured ; Cytokinesis ; Depletion ; Extrusion ; FASCIN ; Female ; Filaments ; Gametocytes ; Localization ; Maturation ; Meiosis ; Metastases ; Mice ; Mice, Inbred ICR ; Microfilament Proteins - genetics ; Microfilament Proteins - metabolism ; oocyte ; Oocytes ; Oocytes - metabolism ; Polar Bodies - metabolism ; Protein kinase C ; Protein Kinase C - metabolism ; rho GTP-Binding Proteins - metabolism ; spindle ; Spindle Apparatus - genetics ; Spindle Apparatus - metabolism ; Spindles ; Therapeutic targets</subject><ispartof>Journal of cellular physiology, 2021-11, Vol.236 (11), p.7725-7733</ispartof><rights>2021 Wiley Periodicals LLC</rights><rights>2021 Wiley Periodicals LLC.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3533-efb3e6a56bfa895e0e6fb8b6a7b33d999e7ada7b8a0c7dffdfc5db2f0cbb83643</citedby><cites>FETCH-LOGICAL-c3533-efb3e6a56bfa895e0e6fb8b6a7b33d999e7ada7b8a0c7dffdfc5db2f0cbb83643</cites><orcidid>0000-0001-5060-1742</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjcp.30443$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjcp.30443$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34018605$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hu, Lin‐Lin</creatorcontrib><creatorcontrib>Pan, Meng‐Hao</creatorcontrib><creatorcontrib>Yang, Feng‐Lian</creatorcontrib><creatorcontrib>Zong, Zi‐Ao</creatorcontrib><creatorcontrib>Tang, Feng</creatorcontrib><creatorcontrib>Pan, Zhen‐Nan</creatorcontrib><creatorcontrib>Lu, Xiang</creatorcontrib><creatorcontrib>Ren, Yan‐Ping</creatorcontrib><creatorcontrib>Wang, Jun‐Li</creatorcontrib><creatorcontrib>Sun, Shao‐Chen</creatorcontrib><title>FASCIN regulates actin assembly for spindle movement and polar body extrusion in mouse oocyte meiosis</title><title>Journal of cellular physiology</title><addtitle>J Cell Physiol</addtitle><description>During mouse oocyte meiotic maturation, actin filaments play multiple roles in meiosis such as spindle migration and cytokinesis. FASCIN is shown to be an actin‐binding and bundling protein, making actin filaments tightly packed and parallel‐aligned, and FASCIN is involved in several cellular processes like adhesion and migration. FASCIN is also a potential prognostic biomarker and therapeutic target for the treatment of metastatic disease. However, little is known about the functions of FASCIN in oocyte meiosis. In the present study, we knocked down the expression of FASCIN, and our results showed that FASCIN was essential for oocyte maturation. FASCIN was all expressed in the different stages of oocyte meiosis, and it mainly localized at the cortex of oocytes from the GV stage to the MII stage and showed a similar localization pattern with actin and DAAM1. Depletion of FASCIN affected the extrusion of the first polar body, and we also observed that some oocytes extruded from the large polar bodies. This might have resulted from the defects of actin assembly, which further affected the meiotic spindle positioning. In addition, we showed that inhibition of PKC activity decreased FASCIN expression, indicating that FASCIN might be regulated by PKC. Taken together, our results provided evidence for the important role of FASCIN on actin filaments for spindle migration and polar body extrusion in mouse oocyte meiosis.
Our results provided evidence for the important roles of FASCIN on actin filaments for spindle migration and polar body extrusion in mouse oocyte meiosis.</description><subject>Actin</subject><subject>Actin Cytoskeleton - genetics</subject><subject>Actin Cytoskeleton - metabolism</subject><subject>Animals</subject><subject>Assembly</subject><subject>Biomarkers</subject><subject>Carrier Proteins - genetics</subject><subject>Carrier Proteins - metabolism</subject><subject>Cells, Cultured</subject><subject>Cytokinesis</subject><subject>Depletion</subject><subject>Extrusion</subject><subject>FASCIN</subject><subject>Female</subject><subject>Filaments</subject><subject>Gametocytes</subject><subject>Localization</subject><subject>Maturation</subject><subject>Meiosis</subject><subject>Metastases</subject><subject>Mice</subject><subject>Mice, Inbred ICR</subject><subject>Microfilament Proteins - genetics</subject><subject>Microfilament Proteins - metabolism</subject><subject>oocyte</subject><subject>Oocytes</subject><subject>Oocytes - metabolism</subject><subject>Polar Bodies - metabolism</subject><subject>Protein kinase C</subject><subject>Protein Kinase C - metabolism</subject><subject>rho GTP-Binding Proteins - metabolism</subject><subject>spindle</subject><subject>Spindle Apparatus - genetics</subject><subject>Spindle Apparatus - metabolism</subject><subject>Spindles</subject><subject>Therapeutic targets</subject><issn>0021-9541</issn><issn>1097-4652</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp10F1LwzAUBuAgipvTC_-ABLzRi25p04_0cgynk6GCel3ycSIdbVOTVu2_N7rpheBVOOQ5L4cXodOQTENCotlGtlNK4pjuoXFI8iyI0yTaR2P_FwZ5EocjdOTchhCS55QeohGNSchSkowRLOePi9UdtvDSV7wDh7nsygZz56AW1YC1sdi1ZaMqwLV5gxqaDvNG4dZU3GJh1IDho7O9K02D_WZtegfYGDl0fgNK40p3jA40rxyc7N4Jel5ePS1ugvX99WoxXweSJpQGoAWFlCep0JzlCRBItWAi5ZmgVOV5DhlXfmCcyExprbRMlIg0kUIwmsZ0gi62ua01rz24rqhLJ6GqeAP-rCJKaBhFNGPE0_M_dGN62_jrvGI0ZnHKmFeXWyWtcc6CLlpb1twORUiKr-4L333x3b23Z7vEXtSgfuVP2R7MtuC9rGD4P6m4XTxsIz8BsYOPbA</recordid><startdate>202111</startdate><enddate>202111</enddate><creator>Hu, Lin‐Lin</creator><creator>Pan, Meng‐Hao</creator><creator>Yang, Feng‐Lian</creator><creator>Zong, Zi‐Ao</creator><creator>Tang, Feng</creator><creator>Pan, Zhen‐Nan</creator><creator>Lu, Xiang</creator><creator>Ren, Yan‐Ping</creator><creator>Wang, Jun‐Li</creator><creator>Sun, Shao‐Chen</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>K9.</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-5060-1742</orcidid></search><sort><creationdate>202111</creationdate><title>FASCIN regulates actin assembly for spindle movement and polar body extrusion in mouse oocyte meiosis</title><author>Hu, Lin‐Lin ; Pan, Meng‐Hao ; Yang, Feng‐Lian ; Zong, Zi‐Ao ; Tang, Feng ; Pan, Zhen‐Nan ; Lu, Xiang ; Ren, Yan‐Ping ; Wang, Jun‐Li ; Sun, Shao‐Chen</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3533-efb3e6a56bfa895e0e6fb8b6a7b33d999e7ada7b8a0c7dffdfc5db2f0cbb83643</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Actin</topic><topic>Actin Cytoskeleton - genetics</topic><topic>Actin Cytoskeleton - metabolism</topic><topic>Animals</topic><topic>Assembly</topic><topic>Biomarkers</topic><topic>Carrier Proteins - genetics</topic><topic>Carrier Proteins - metabolism</topic><topic>Cells, Cultured</topic><topic>Cytokinesis</topic><topic>Depletion</topic><topic>Extrusion</topic><topic>FASCIN</topic><topic>Female</topic><topic>Filaments</topic><topic>Gametocytes</topic><topic>Localization</topic><topic>Maturation</topic><topic>Meiosis</topic><topic>Metastases</topic><topic>Mice</topic><topic>Mice, Inbred ICR</topic><topic>Microfilament Proteins - genetics</topic><topic>Microfilament Proteins - metabolism</topic><topic>oocyte</topic><topic>Oocytes</topic><topic>Oocytes - metabolism</topic><topic>Polar Bodies - metabolism</topic><topic>Protein kinase C</topic><topic>Protein Kinase C - metabolism</topic><topic>rho GTP-Binding Proteins - metabolism</topic><topic>spindle</topic><topic>Spindle Apparatus - genetics</topic><topic>Spindle Apparatus - metabolism</topic><topic>Spindles</topic><topic>Therapeutic targets</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hu, Lin‐Lin</creatorcontrib><creatorcontrib>Pan, Meng‐Hao</creatorcontrib><creatorcontrib>Yang, Feng‐Lian</creatorcontrib><creatorcontrib>Zong, Zi‐Ao</creatorcontrib><creatorcontrib>Tang, Feng</creatorcontrib><creatorcontrib>Pan, Zhen‐Nan</creatorcontrib><creatorcontrib>Lu, Xiang</creatorcontrib><creatorcontrib>Ren, Yan‐Ping</creatorcontrib><creatorcontrib>Wang, Jun‐Li</creatorcontrib><creatorcontrib>Sun, Shao‐Chen</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of cellular physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hu, Lin‐Lin</au><au>Pan, Meng‐Hao</au><au>Yang, Feng‐Lian</au><au>Zong, Zi‐Ao</au><au>Tang, Feng</au><au>Pan, Zhen‐Nan</au><au>Lu, Xiang</au><au>Ren, Yan‐Ping</au><au>Wang, Jun‐Li</au><au>Sun, Shao‐Chen</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>FASCIN regulates actin assembly for spindle movement and polar body extrusion in mouse oocyte meiosis</atitle><jtitle>Journal of cellular physiology</jtitle><addtitle>J Cell Physiol</addtitle><date>2021-11</date><risdate>2021</risdate><volume>236</volume><issue>11</issue><spage>7725</spage><epage>7733</epage><pages>7725-7733</pages><issn>0021-9541</issn><eissn>1097-4652</eissn><abstract>During mouse oocyte meiotic maturation, actin filaments play multiple roles in meiosis such as spindle migration and cytokinesis. FASCIN is shown to be an actin‐binding and bundling protein, making actin filaments tightly packed and parallel‐aligned, and FASCIN is involved in several cellular processes like adhesion and migration. FASCIN is also a potential prognostic biomarker and therapeutic target for the treatment of metastatic disease. However, little is known about the functions of FASCIN in oocyte meiosis. In the present study, we knocked down the expression of FASCIN, and our results showed that FASCIN was essential for oocyte maturation. FASCIN was all expressed in the different stages of oocyte meiosis, and it mainly localized at the cortex of oocytes from the GV stage to the MII stage and showed a similar localization pattern with actin and DAAM1. Depletion of FASCIN affected the extrusion of the first polar body, and we also observed that some oocytes extruded from the large polar bodies. This might have resulted from the defects of actin assembly, which further affected the meiotic spindle positioning. In addition, we showed that inhibition of PKC activity decreased FASCIN expression, indicating that FASCIN might be regulated by PKC. Taken together, our results provided evidence for the important role of FASCIN on actin filaments for spindle migration and polar body extrusion in mouse oocyte meiosis.
Our results provided evidence for the important roles of FASCIN on actin filaments for spindle migration and polar body extrusion in mouse oocyte meiosis.</abstract><cop>United States</cop><pub>Wiley Subscription Services, Inc</pub><pmid>34018605</pmid><doi>10.1002/jcp.30443</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0001-5060-1742</orcidid></addata></record> |
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| subjects | Actin Actin Cytoskeleton - genetics Actin Cytoskeleton - metabolism Animals Assembly Biomarkers Carrier Proteins - genetics Carrier Proteins - metabolism Cells, Cultured Cytokinesis Depletion Extrusion FASCIN Female Filaments Gametocytes Localization Maturation Meiosis Metastases Mice Mice, Inbred ICR Microfilament Proteins - genetics Microfilament Proteins - metabolism oocyte Oocytes Oocytes - metabolism Polar Bodies - metabolism Protein kinase C Protein Kinase C - metabolism rho GTP-Binding Proteins - metabolism spindle Spindle Apparatus - genetics Spindle Apparatus - metabolism Spindles Therapeutic targets |
| title | FASCIN regulates actin assembly for spindle movement and polar body extrusion in mouse oocyte meiosis |
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