Improving the activity and stability of Bacillus clausii alkaline protease using directed evolution and molecular dynamics simulation

•B. clausii alkaline protease (PRO) mutant with better properties was obtained.•The PRO mutant demonstrated a 9 times specific activity of wild type at 60 °C.•The PRO mutant possessed improved thermostability and alkaline stability.•Molecular dynamics clarified the mechanism of PRO mutant with impro...

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Veröffentlicht in:	Enzyme and microbial technology 2021-06, Vol.147, p.109787-109787, Article 109787
Hauptverfasser:	Li, Jialin, Jiang, Luying, Cao, Xue, Wu, Yifan, Lu, Fuping, Liu, Fufeng, Li, Yu, Liu, Yihan
Format:	Artikel
Sprache:	eng
Schlagworte:	Alkaline protease Alkaline stability Molecular dynamics simulation Specific activity Thermostability
Online-Zugang:	Volltext
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