Mkx regulates the orthodontic tooth movement via osteoclast induction
Introduction The periodontal ligament (PDL) plays an important role in orthodontic tooth movement; however, the underlying molecular mechanism remains unclear. We have previously reported that the Mohawk homeobox ( Mkx ), a tendon-specific transcription factor, is expressed in the PDL and regulates...
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| Veröffentlicht in: | Journal of bone and mineral metabolism 2021-09, Vol.39 (5), p.780-786 |
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| creator | Miyazaki, Takayuki Kurimoto, Ryota Chiba, Tomoki Matsushima, Takahide Nakamichi, Ryo Tsutsumi, Hiroki Takada, Kaho Yagasaki, Lisa Kato, Tomomi Shishido, Kana Kobayashi, Yukiho Matsumoto, Tsutomu Moriyama, Keiji Asahara, Hiroshi |
| description | Introduction
The periodontal ligament (PDL) plays an important role in orthodontic tooth movement; however, the underlying molecular mechanism remains unclear. We have previously reported that the Mohawk homeobox (
Mkx
), a tendon-specific transcription factor, is expressed in the PDL and regulates its homeostasis.
Materials and methods
In the present study, we examined the role of
Mkx
in orthodontic tooth movement via bone remodeling induced by mechanical stimulation in
Mkx
-deficient rats, which are widely used as experimental animals for orthodontic force application. Orthodontic tooth movement of the maxillary first molar was performed in 7-week-old male
Mkx
-deficient rats (
n
= 4) and wild-type Wistar rats (
n
= 4) using coil springs for 14 days. Hematoxylin and eosin (H&E) staining and tartrate-resistant acid phosphatase (TRAP) staining were performed to evaluate morphological changes and osteoclasts. Furthermore, changes in the expression of receptor activator nuclear factor-kappa B ligand (RANKL) were demonstrated using immunostaining.
Results
The amount of tooth movement was significantly lower in
Mkx
-deficient rats than in wild-type rats. The number of TRAP-positive cells was suppressed in
Mkx
-deficient rats on the compression side.
Conclusion
Orthodontic tooth movement experiments in
Mkx
-deficient rats suggested that
Mkx
is involved in osteoclast induction at the alveolar bone surface on the compression side. This study reveals the possibility that Mkx plays a mechanosensory role in orthodontic tooth movement by inducing RANKL expression and osteoclastogenesis. |
| doi_str_mv | 10.1007/s00774-021-01233-2 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2528179692</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2528179692</sourcerecordid><originalsourceid>FETCH-LOGICAL-c549t-7ffaa6988ddc997afaeae3189d572444437ac4b645ce57a6f873836135434d9e3</originalsourceid><addsrcrecordid>eNp9kD1PwzAURS0EglL4AwwoEgtLwJ9xPCJUPqQiFpgt13lpA0lcbKeCf49LC0gMvMFv8PG91kHohOALgrG8DOmQPMeU5JhQxnK6g0aEM5GLAvNdNMKK8LyUUh2gwxBeMCZSSLKPDhhTZSmFGKHJw-t75mE-tCZCyOICMufjwlWuj43NonNxkXVuBR30MVs1JnMhgrOtCTFr-mqwsXH9EdqrTRvgeLvH6Plm8nR9l08fb--vr6a5FVzFXNa1MUWqriqrlDS1AQOMlKoSkvI0TBrLZwUXFoQ0RV1KVrKCMMEZrxSwMTrf5C69exsgRN01wULbmh7cEDQVtCRSFYom9OwP-uIG36ffJaoQilGiykTRDWW9C8FDrZe-6Yz_0ATrtWS9kayTZP0lWa-jT7fRw6yD6ufJt9UEsA0Q0lU_B__b_U_sJ26hhwc</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2565932198</pqid></control><display><type>article</type><title>Mkx regulates the orthodontic tooth movement via osteoclast induction</title><source>SpringerLink Journals</source><creator>Miyazaki, Takayuki ; Kurimoto, Ryota ; Chiba, Tomoki ; Matsushima, Takahide ; Nakamichi, Ryo ; Tsutsumi, Hiroki ; Takada, Kaho ; Yagasaki, Lisa ; Kato, Tomomi ; Shishido, Kana ; Kobayashi, Yukiho ; Matsumoto, Tsutomu ; Moriyama, Keiji ; Asahara, Hiroshi</creator><creatorcontrib>Miyazaki, Takayuki ; Kurimoto, Ryota ; Chiba, Tomoki ; Matsushima, Takahide ; Nakamichi, Ryo ; Tsutsumi, Hiroki ; Takada, Kaho ; Yagasaki, Lisa ; Kato, Tomomi ; Shishido, Kana ; Kobayashi, Yukiho ; Matsumoto, Tsutomu ; Moriyama, Keiji ; Asahara, Hiroshi</creatorcontrib><description>Introduction
The periodontal ligament (PDL) plays an important role in orthodontic tooth movement; however, the underlying molecular mechanism remains unclear. We have previously reported that the Mohawk homeobox (
Mkx
), a tendon-specific transcription factor, is expressed in the PDL and regulates its homeostasis.
Materials and methods
In the present study, we examined the role of
Mkx
in orthodontic tooth movement via bone remodeling induced by mechanical stimulation in
Mkx
-deficient rats, which are widely used as experimental animals for orthodontic force application. Orthodontic tooth movement of the maxillary first molar was performed in 7-week-old male
Mkx
-deficient rats (
n
= 4) and wild-type Wistar rats (
n
= 4) using coil springs for 14 days. Hematoxylin and eosin (H&E) staining and tartrate-resistant acid phosphatase (TRAP) staining were performed to evaluate morphological changes and osteoclasts. Furthermore, changes in the expression of receptor activator nuclear factor-kappa B ligand (RANKL) were demonstrated using immunostaining.
Results
The amount of tooth movement was significantly lower in
Mkx
-deficient rats than in wild-type rats. The number of TRAP-positive cells was suppressed in
Mkx
-deficient rats on the compression side.
Conclusion
Orthodontic tooth movement experiments in
Mkx
-deficient rats suggested that
Mkx
is involved in osteoclast induction at the alveolar bone surface on the compression side. This study reveals the possibility that Mkx plays a mechanosensory role in orthodontic tooth movement by inducing RANKL expression and osteoclastogenesis.</description><identifier>ISSN: 0914-8779</identifier><identifier>EISSN: 1435-5604</identifier><identifier>DOI: 10.1007/s00774-021-01233-2</identifier><identifier>PMID: 33988755</identifier><language>eng</language><publisher>Singapore: Springer Singapore</publisher><subject>Acid phosphatase (tartrate-resistant) ; Alveolar bone ; Bone remodeling ; Compression ; Homeobox ; Homeostasis ; Mechanical stimuli ; Medicine ; Medicine & Public Health ; Metabolic Diseases ; NF-κB protein ; Original Article ; Orthodontics ; Orthopedics ; Osteoclastogenesis ; Osteoclasts ; Periodontal ligament ; Teeth ; TRANCE protein</subject><ispartof>Journal of bone and mineral metabolism, 2021-09, Vol.39 (5), p.780-786</ispartof><rights>The Japanese Society Bone and Mineral Research 2021</rights><rights>The Japanese Society Bone and Mineral Research 2021.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c549t-7ffaa6988ddc997afaeae3189d572444437ac4b645ce57a6f873836135434d9e3</citedby><cites>FETCH-LOGICAL-c549t-7ffaa6988ddc997afaeae3189d572444437ac4b645ce57a6f873836135434d9e3</cites><orcidid>0000-0002-5215-8745</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00774-021-01233-2$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00774-021-01233-2$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,777,781,27905,27906,41469,42538,51300</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33988755$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Miyazaki, Takayuki</creatorcontrib><creatorcontrib>Kurimoto, Ryota</creatorcontrib><creatorcontrib>Chiba, Tomoki</creatorcontrib><creatorcontrib>Matsushima, Takahide</creatorcontrib><creatorcontrib>Nakamichi, Ryo</creatorcontrib><creatorcontrib>Tsutsumi, Hiroki</creatorcontrib><creatorcontrib>Takada, Kaho</creatorcontrib><creatorcontrib>Yagasaki, Lisa</creatorcontrib><creatorcontrib>Kato, Tomomi</creatorcontrib><creatorcontrib>Shishido, Kana</creatorcontrib><creatorcontrib>Kobayashi, Yukiho</creatorcontrib><creatorcontrib>Matsumoto, Tsutomu</creatorcontrib><creatorcontrib>Moriyama, Keiji</creatorcontrib><creatorcontrib>Asahara, Hiroshi</creatorcontrib><title>Mkx regulates the orthodontic tooth movement via osteoclast induction</title><title>Journal of bone and mineral metabolism</title><addtitle>J Bone Miner Metab</addtitle><addtitle>J Bone Miner Metab</addtitle><description>Introduction
The periodontal ligament (PDL) plays an important role in orthodontic tooth movement; however, the underlying molecular mechanism remains unclear. We have previously reported that the Mohawk homeobox (
Mkx
), a tendon-specific transcription factor, is expressed in the PDL and regulates its homeostasis.
Materials and methods
In the present study, we examined the role of
Mkx
in orthodontic tooth movement via bone remodeling induced by mechanical stimulation in
Mkx
-deficient rats, which are widely used as experimental animals for orthodontic force application. Orthodontic tooth movement of the maxillary first molar was performed in 7-week-old male
Mkx
-deficient rats (
n
= 4) and wild-type Wistar rats (
n
= 4) using coil springs for 14 days. Hematoxylin and eosin (H&E) staining and tartrate-resistant acid phosphatase (TRAP) staining were performed to evaluate morphological changes and osteoclasts. Furthermore, changes in the expression of receptor activator nuclear factor-kappa B ligand (RANKL) were demonstrated using immunostaining.
Results
The amount of tooth movement was significantly lower in
Mkx
-deficient rats than in wild-type rats. The number of TRAP-positive cells was suppressed in
Mkx
-deficient rats on the compression side.
Conclusion
Orthodontic tooth movement experiments in
Mkx
-deficient rats suggested that
Mkx
is involved in osteoclast induction at the alveolar bone surface on the compression side. This study reveals the possibility that Mkx plays a mechanosensory role in orthodontic tooth movement by inducing RANKL expression and osteoclastogenesis.</description><subject>Acid phosphatase (tartrate-resistant)</subject><subject>Alveolar bone</subject><subject>Bone remodeling</subject><subject>Compression</subject><subject>Homeobox</subject><subject>Homeostasis</subject><subject>Mechanical stimuli</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Metabolic Diseases</subject><subject>NF-κB protein</subject><subject>Original Article</subject><subject>Orthodontics</subject><subject>Orthopedics</subject><subject>Osteoclastogenesis</subject><subject>Osteoclasts</subject><subject>Periodontal ligament</subject><subject>Teeth</subject><subject>TRANCE protein</subject><issn>0914-8779</issn><issn>1435-5604</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><recordid>eNp9kD1PwzAURS0EglL4AwwoEgtLwJ9xPCJUPqQiFpgt13lpA0lcbKeCf49LC0gMvMFv8PG91kHohOALgrG8DOmQPMeU5JhQxnK6g0aEM5GLAvNdNMKK8LyUUh2gwxBeMCZSSLKPDhhTZSmFGKHJw-t75mE-tCZCyOICMufjwlWuj43NonNxkXVuBR30MVs1JnMhgrOtCTFr-mqwsXH9EdqrTRvgeLvH6Plm8nR9l08fb--vr6a5FVzFXNa1MUWqriqrlDS1AQOMlKoSkvI0TBrLZwUXFoQ0RV1KVrKCMMEZrxSwMTrf5C69exsgRN01wULbmh7cEDQVtCRSFYom9OwP-uIG36ffJaoQilGiykTRDWW9C8FDrZe-6Yz_0ATrtWS9kayTZP0lWa-jT7fRw6yD6ufJt9UEsA0Q0lU_B__b_U_sJ26hhwc</recordid><startdate>20210901</startdate><enddate>20210901</enddate><creator>Miyazaki, Takayuki</creator><creator>Kurimoto, Ryota</creator><creator>Chiba, Tomoki</creator><creator>Matsushima, Takahide</creator><creator>Nakamichi, Ryo</creator><creator>Tsutsumi, Hiroki</creator><creator>Takada, Kaho</creator><creator>Yagasaki, Lisa</creator><creator>Kato, Tomomi</creator><creator>Shishido, Kana</creator><creator>Kobayashi, Yukiho</creator><creator>Matsumoto, Tsutomu</creator><creator>Moriyama, Keiji</creator><creator>Asahara, Hiroshi</creator><general>Springer Singapore</general><general>Springer Nature B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QP</scope><scope>7RV</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>KB0</scope><scope>M0S</scope><scope>M1P</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-5215-8745</orcidid></search><sort><creationdate>20210901</creationdate><title>Mkx regulates the orthodontic tooth movement via osteoclast induction</title><author>Miyazaki, Takayuki ; Kurimoto, Ryota ; Chiba, Tomoki ; Matsushima, Takahide ; Nakamichi, Ryo ; Tsutsumi, Hiroki ; Takada, Kaho ; Yagasaki, Lisa ; Kato, Tomomi ; Shishido, Kana ; Kobayashi, Yukiho ; Matsumoto, Tsutomu ; Moriyama, Keiji ; Asahara, Hiroshi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c549t-7ffaa6988ddc997afaeae3189d572444437ac4b645ce57a6f873836135434d9e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Acid phosphatase (tartrate-resistant)</topic><topic>Alveolar bone</topic><topic>Bone remodeling</topic><topic>Compression</topic><topic>Homeobox</topic><topic>Homeostasis</topic><topic>Mechanical stimuli</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Metabolic Diseases</topic><topic>NF-κB protein</topic><topic>Original Article</topic><topic>Orthodontics</topic><topic>Orthopedics</topic><topic>Osteoclastogenesis</topic><topic>Osteoclasts</topic><topic>Periodontal ligament</topic><topic>Teeth</topic><topic>TRANCE protein</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Miyazaki, Takayuki</creatorcontrib><creatorcontrib>Kurimoto, Ryota</creatorcontrib><creatorcontrib>Chiba, Tomoki</creatorcontrib><creatorcontrib>Matsushima, Takahide</creatorcontrib><creatorcontrib>Nakamichi, Ryo</creatorcontrib><creatorcontrib>Tsutsumi, Hiroki</creatorcontrib><creatorcontrib>Takada, Kaho</creatorcontrib><creatorcontrib>Yagasaki, Lisa</creatorcontrib><creatorcontrib>Kato, Tomomi</creatorcontrib><creatorcontrib>Shishido, Kana</creatorcontrib><creatorcontrib>Kobayashi, Yukiho</creatorcontrib><creatorcontrib>Matsumoto, Tsutomu</creatorcontrib><creatorcontrib>Moriyama, Keiji</creatorcontrib><creatorcontrib>Asahara, Hiroshi</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of bone and mineral metabolism</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Miyazaki, Takayuki</au><au>Kurimoto, Ryota</au><au>Chiba, Tomoki</au><au>Matsushima, Takahide</au><au>Nakamichi, Ryo</au><au>Tsutsumi, Hiroki</au><au>Takada, Kaho</au><au>Yagasaki, Lisa</au><au>Kato, Tomomi</au><au>Shishido, Kana</au><au>Kobayashi, Yukiho</au><au>Matsumoto, Tsutomu</au><au>Moriyama, Keiji</au><au>Asahara, Hiroshi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Mkx regulates the orthodontic tooth movement via osteoclast induction</atitle><jtitle>Journal of bone and mineral metabolism</jtitle><stitle>J Bone Miner Metab</stitle><addtitle>J Bone Miner Metab</addtitle><date>2021-09-01</date><risdate>2021</risdate><volume>39</volume><issue>5</issue><spage>780</spage><epage>786</epage><pages>780-786</pages><issn>0914-8779</issn><eissn>1435-5604</eissn><abstract>Introduction
The periodontal ligament (PDL) plays an important role in orthodontic tooth movement; however, the underlying molecular mechanism remains unclear. We have previously reported that the Mohawk homeobox (
Mkx
), a tendon-specific transcription factor, is expressed in the PDL and regulates its homeostasis.
Materials and methods
In the present study, we examined the role of
Mkx
in orthodontic tooth movement via bone remodeling induced by mechanical stimulation in
Mkx
-deficient rats, which are widely used as experimental animals for orthodontic force application. Orthodontic tooth movement of the maxillary first molar was performed in 7-week-old male
Mkx
-deficient rats (
n
= 4) and wild-type Wistar rats (
n
= 4) using coil springs for 14 days. Hematoxylin and eosin (H&E) staining and tartrate-resistant acid phosphatase (TRAP) staining were performed to evaluate morphological changes and osteoclasts. Furthermore, changes in the expression of receptor activator nuclear factor-kappa B ligand (RANKL) were demonstrated using immunostaining.
Results
The amount of tooth movement was significantly lower in
Mkx
-deficient rats than in wild-type rats. The number of TRAP-positive cells was suppressed in
Mkx
-deficient rats on the compression side.
Conclusion
Orthodontic tooth movement experiments in
Mkx
-deficient rats suggested that
Mkx
is involved in osteoclast induction at the alveolar bone surface on the compression side. This study reveals the possibility that Mkx plays a mechanosensory role in orthodontic tooth movement by inducing RANKL expression and osteoclastogenesis.</abstract><cop>Singapore</cop><pub>Springer Singapore</pub><pmid>33988755</pmid><doi>10.1007/s00774-021-01233-2</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0002-5215-8745</orcidid></addata></record> |
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| language | eng |
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| source | SpringerLink Journals |
| subjects | Acid phosphatase (tartrate-resistant) Alveolar bone Bone remodeling Compression Homeobox Homeostasis Mechanical stimuli Medicine Medicine & Public Health Metabolic Diseases NF-κB protein Original Article Orthodontics Orthopedics Osteoclastogenesis Osteoclasts Periodontal ligament Teeth TRANCE protein |
| title | Mkx regulates the orthodontic tooth movement via osteoclast induction |
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