Development of an absolute quantification method for ribosomal RNA gene copy numbers per eukaryotic single cell by digital PCR

•Application of a new universal primer-probe set targeting the 18S rRNA gene.•Absolute quantification of ribosomal RNA gene copy numbers in microalgae by dPCR.•The copy numbers varied from 44 - 2,031,500 copies cell-1 in 16 microalgae species.•Dinoflagellates had more ribosomal RNA copies per cell t...

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Veröffentlicht in:	Harmful algae 2021-03, Vol.103, p.102008-102008, Article 102008
Hauptverfasser:	Yarimizu, Kyoko, Sildever, Sirje, Hamamoto, Yoko, Tazawa, Satoshi, Oikawa, Hiroshi, Yamaguchi, Haruo, Basti, Leila, Mardones, Jorge I., Paredes-Mella, Javier, Nagai, Satoshi
Format:	Artikel
Sprache:	eng
Schlagworte:	Absolute quantification Digital PCR Eukaryotes HAB Phytoplankton rRNA gene copy number Universal primer and probe
Online-Zugang:	Volltext
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