Microbiological and SEM assessment of atraumatic restorative treatment in adult dentition
Objectives To investigate the practicability of atraumatic restorative treatment (ART) in adults in terms of marginal adaptation of restorations and microbiological changes in residual carious dentin. Materials and methods The occlusal dentin caries of 25 permanent molar teeth were removed with hand...
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| Veröffentlicht in: | Clinical oral investigations 2021-12, Vol.25 (12), p.6871-6880 |
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| container_title | Clinical oral investigations |
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| creator | Tekbas Atay, Meltem Koray, Fatma |
| description | Objectives
To investigate the practicability of atraumatic restorative treatment (ART) in adults in terms of marginal adaptation of restorations and microbiological changes in residual carious dentin.
Materials and methods
The occlusal dentin caries of 25 permanent molar teeth were removed with hand instruments. The total counts of bacteria (TCB) and the facultative
anaerobic
bacteria (FAB), mutans streptococci (MS), and
Lactobacillus
spp. (LB) counts in the affected dentin were evaluated quantitatively. The weights of the samples were measured with an electronic balance (Shimadzu, Type AX200, Japan). The cavities were restored with glass ionomer cement (Ketac
TM
Molar Easymix, ESPE Dental AG, Seefeld, Germany). Twenty replicas of randomly selected ART restorations were prepared and marginal adaptation was evaluated by scanning electron microscopy (SEM). After 6 months, the same protocols were repeated. Data were analyzed with paired sample
t
-tests, Wilcoxon
t
-tests, Pearson and Spearman correlations, and chi-square tests (
p |
| doi_str_mv | 10.1007/s00784-021-03976-8 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2526306752</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2598833858</sourcerecordid><originalsourceid>FETCH-LOGICAL-c326t-441bb678b1f8559ecc46de83091287bc4b495d622b5e8dae1f2e4ad8a1ce70343</originalsourceid><addsrcrecordid>eNp9kMtKxjAQRoMo3l_AhQTcuKnm1iRdingDxYW6cBXSdCqRttEkFXx74_97ARdukgk5881wENqj5IgSoo5TObSoCKMV4Y2SlV5Bm1RwWXGl6OqiZpVsNN1AWyk9E0KFVHwdbXDeaEal2kSPN97F0PowhCfv7IDt1OG7sxtsU4KURpgyDj22Odp5tNk7HCHlEEv5BjhHsHnB-Anbbh4y7srLZx-mHbTW2yHB7te9jR7Oz-5PL6vr24ur05PrynEmcyUEbVupdEt7XdcNOCdkB5qThjKtWida0dSdZKytQXcWaM9A2E5b6kARLvg2OlzmvsTwOpflzOiTg2GwE4Q5GVYzyYlUNSvowR_0OcxxKtsVqtGac13rQrElVcSkFKE3L9GPNr4bSsyneLMUb4p4sxBvPpv2v6LndoTup-XbdAH4Ekjla3qC-Dv7n9gPIWeOng</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2598833858</pqid></control><display><type>article</type><title>Microbiological and SEM assessment of atraumatic restorative treatment in adult dentition</title><source>Springer Nature - Complete Springer Journals</source><creator>Tekbas Atay, Meltem ; Koray, Fatma</creator><creatorcontrib>Tekbas Atay, Meltem ; Koray, Fatma</creatorcontrib><description>Objectives
To investigate the practicability of atraumatic restorative treatment (ART) in adults in terms of marginal adaptation of restorations and microbiological changes in residual carious dentin.
Materials and methods
The occlusal dentin caries of 25 permanent molar teeth were removed with hand instruments. The total counts of bacteria (TCB) and the facultative
anaerobic
bacteria (FAB), mutans streptococci (MS), and
Lactobacillus
spp. (LB) counts in the affected dentin were evaluated quantitatively. The weights of the samples were measured with an electronic balance (Shimadzu, Type AX200, Japan). The cavities were restored with glass ionomer cement (Ketac
TM
Molar Easymix, ESPE Dental AG, Seefeld, Germany). Twenty replicas of randomly selected ART restorations were prepared and marginal adaptation was evaluated by scanning electron microscopy (SEM). After 6 months, the same protocols were repeated. Data were analyzed with paired sample
t
-tests, Wilcoxon
t
-tests, Pearson and Spearman correlations, and chi-square tests (
p
<0.05).
Results
In the sixth month, restoration loss and pulpitis were not observed. The mean weight of samples removed from the cavity floor was less than the baseline (0.014±0.009 and 0.023±0.013 g, respectively) (
p
<0.01), and the counts of total bacteria, FAB, MS, and LB significantly decreased compared to baseline (
p
<0.01). The frequency of marginal gaps was increased (
p
< 0.01).
Conclusions
ART showed that the counts of microorganisms decreased after 6 months although the marginal gap rates of restorations increased.
Clinical relevance
ART can be a reliable treatment approach in adults for 6 months due to the decrease in microorganism counts, although gaps exist.</description><identifier>ISSN: 1432-6981</identifier><identifier>EISSN: 1436-3771</identifier><identifier>DOI: 10.1007/s00784-021-03976-8</identifier><identifier>PMID: 33982167</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Anaerobic bacteria ; Bacteria ; Dental caries ; Dentin ; Dentistry ; Dentition ; Medicine ; Microorganisms ; Original Article ; Scanning electron microscopy ; Teeth</subject><ispartof>Clinical oral investigations, 2021-12, Vol.25 (12), p.6871-6880</ispartof><rights>The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2021</rights><rights>The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2021.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c326t-441bb678b1f8559ecc46de83091287bc4b495d622b5e8dae1f2e4ad8a1ce70343</cites><orcidid>0000-0002-1762-830X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00784-021-03976-8$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00784-021-03976-8$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,778,782,27911,27912,41475,42544,51306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33982167$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tekbas Atay, Meltem</creatorcontrib><creatorcontrib>Koray, Fatma</creatorcontrib><title>Microbiological and SEM assessment of atraumatic restorative treatment in adult dentition</title><title>Clinical oral investigations</title><addtitle>Clin Oral Invest</addtitle><addtitle>Clin Oral Investig</addtitle><description>Objectives
To investigate the practicability of atraumatic restorative treatment (ART) in adults in terms of marginal adaptation of restorations and microbiological changes in residual carious dentin.
Materials and methods
The occlusal dentin caries of 25 permanent molar teeth were removed with hand instruments. The total counts of bacteria (TCB) and the facultative
anaerobic
bacteria (FAB), mutans streptococci (MS), and
Lactobacillus
spp. (LB) counts in the affected dentin were evaluated quantitatively. The weights of the samples were measured with an electronic balance (Shimadzu, Type AX200, Japan). The cavities were restored with glass ionomer cement (Ketac
TM
Molar Easymix, ESPE Dental AG, Seefeld, Germany). Twenty replicas of randomly selected ART restorations were prepared and marginal adaptation was evaluated by scanning electron microscopy (SEM). After 6 months, the same protocols were repeated. Data were analyzed with paired sample
t
-tests, Wilcoxon
t
-tests, Pearson and Spearman correlations, and chi-square tests (
p
<0.05).
Results
In the sixth month, restoration loss and pulpitis were not observed. The mean weight of samples removed from the cavity floor was less than the baseline (0.014±0.009 and 0.023±0.013 g, respectively) (
p
<0.01), and the counts of total bacteria, FAB, MS, and LB significantly decreased compared to baseline (
p
<0.01). The frequency of marginal gaps was increased (
p
< 0.01).
Conclusions
ART showed that the counts of microorganisms decreased after 6 months although the marginal gap rates of restorations increased.
Clinical relevance
ART can be a reliable treatment approach in adults for 6 months due to the decrease in microorganism counts, although gaps exist.</description><subject>Anaerobic bacteria</subject><subject>Bacteria</subject><subject>Dental caries</subject><subject>Dentin</subject><subject>Dentistry</subject><subject>Dentition</subject><subject>Medicine</subject><subject>Microorganisms</subject><subject>Original Article</subject><subject>Scanning electron microscopy</subject><subject>Teeth</subject><issn>1432-6981</issn><issn>1436-3771</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kMtKxjAQRoMo3l_AhQTcuKnm1iRdingDxYW6cBXSdCqRttEkFXx74_97ARdukgk5881wENqj5IgSoo5TObSoCKMV4Y2SlV5Bm1RwWXGl6OqiZpVsNN1AWyk9E0KFVHwdbXDeaEal2kSPN97F0PowhCfv7IDt1OG7sxtsU4KURpgyDj22Odp5tNk7HCHlEEv5BjhHsHnB-Anbbh4y7srLZx-mHbTW2yHB7te9jR7Oz-5PL6vr24ur05PrynEmcyUEbVupdEt7XdcNOCdkB5qThjKtWida0dSdZKytQXcWaM9A2E5b6kARLvg2OlzmvsTwOpflzOiTg2GwE4Q5GVYzyYlUNSvowR_0OcxxKtsVqtGac13rQrElVcSkFKE3L9GPNr4bSsyneLMUb4p4sxBvPpv2v6LndoTup-XbdAH4Ekjla3qC-Dv7n9gPIWeOng</recordid><startdate>20211201</startdate><enddate>20211201</enddate><creator>Tekbas Atay, Meltem</creator><creator>Koray, Fatma</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-1762-830X</orcidid></search><sort><creationdate>20211201</creationdate><title>Microbiological and SEM assessment of atraumatic restorative treatment in adult dentition</title><author>Tekbas Atay, Meltem ; Koray, Fatma</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c326t-441bb678b1f8559ecc46de83091287bc4b495d622b5e8dae1f2e4ad8a1ce70343</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Anaerobic bacteria</topic><topic>Bacteria</topic><topic>Dental caries</topic><topic>Dentin</topic><topic>Dentistry</topic><topic>Dentition</topic><topic>Medicine</topic><topic>Microorganisms</topic><topic>Original Article</topic><topic>Scanning electron microscopy</topic><topic>Teeth</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tekbas Atay, Meltem</creatorcontrib><creatorcontrib>Koray, Fatma</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Clinical oral investigations</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tekbas Atay, Meltem</au><au>Koray, Fatma</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Microbiological and SEM assessment of atraumatic restorative treatment in adult dentition</atitle><jtitle>Clinical oral investigations</jtitle><stitle>Clin Oral Invest</stitle><addtitle>Clin Oral Investig</addtitle><date>2021-12-01</date><risdate>2021</risdate><volume>25</volume><issue>12</issue><spage>6871</spage><epage>6880</epage><pages>6871-6880</pages><issn>1432-6981</issn><eissn>1436-3771</eissn><abstract>Objectives
To investigate the practicability of atraumatic restorative treatment (ART) in adults in terms of marginal adaptation of restorations and microbiological changes in residual carious dentin.
Materials and methods
The occlusal dentin caries of 25 permanent molar teeth were removed with hand instruments. The total counts of bacteria (TCB) and the facultative
anaerobic
bacteria (FAB), mutans streptococci (MS), and
Lactobacillus
spp. (LB) counts in the affected dentin were evaluated quantitatively. The weights of the samples were measured with an electronic balance (Shimadzu, Type AX200, Japan). The cavities were restored with glass ionomer cement (Ketac
TM
Molar Easymix, ESPE Dental AG, Seefeld, Germany). Twenty replicas of randomly selected ART restorations were prepared and marginal adaptation was evaluated by scanning electron microscopy (SEM). After 6 months, the same protocols were repeated. Data were analyzed with paired sample
t
-tests, Wilcoxon
t
-tests, Pearson and Spearman correlations, and chi-square tests (
p
<0.05).
Results
In the sixth month, restoration loss and pulpitis were not observed. The mean weight of samples removed from the cavity floor was less than the baseline (0.014±0.009 and 0.023±0.013 g, respectively) (
p
<0.01), and the counts of total bacteria, FAB, MS, and LB significantly decreased compared to baseline (
p
<0.01). The frequency of marginal gaps was increased (
p
< 0.01).
Conclusions
ART showed that the counts of microorganisms decreased after 6 months although the marginal gap rates of restorations increased.
Clinical relevance
ART can be a reliable treatment approach in adults for 6 months due to the decrease in microorganism counts, although gaps exist.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>33982167</pmid><doi>10.1007/s00784-021-03976-8</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0002-1762-830X</orcidid></addata></record> |
| fulltext | fulltext |
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| ispartof | Clinical oral investigations, 2021-12, Vol.25 (12), p.6871-6880 |
| issn | 1432-6981 1436-3771 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2526306752 |
| source | Springer Nature - Complete Springer Journals |
| subjects | Anaerobic bacteria Bacteria Dental caries Dentin Dentistry Dentition Medicine Microorganisms Original Article Scanning electron microscopy Teeth |
| title | Microbiological and SEM assessment of atraumatic restorative treatment in adult dentition |
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