Identification of the iron-limitation stimulon in Staphylococcus lugdunensis
During the infectious process, pathogens such as Staphylococcus lugdunensis have to cope with the condition of host-induced iron-limitation. Using the RNAseq approach, we performed the first global transcriptomic analysis of S. lugdunensis cells incubated in the absence and presence of iron chelator...
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| Veröffentlicht in: | Archives of microbiology 2021-08, Vol.203 (6), p.3687-3694 |
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| creator | Aubourg, Marion Gravey, François Dhalluin, Anne Giard, Jean-Christophe |
| description | During the infectious process, pathogens such as
Staphylococcus lugdunensis
have to cope with the condition of host-induced iron-limitation. Using the RNAseq approach, we performed the first global transcriptomic analysis of
S. lugdunensis
cells incubated in the absence and presence of iron chelator. One hundred and seventy-five genes were identified as members of the iron-limitation stimulon (127 up- and 48 downregulated). Six gene clusters known or likely required for the acquisition of iron have been identified. Among them, a novel Energy-Coupling Factor type transporter (ECF), homologous to the
lhaSTA
operon, has been found into a 13-gene putative operon and strongly overexpressed under iron-limitation condition. Moreover, the transcription of genes involved in resistance to oxidative stress (including catalase), virulence, transcriptional regulation, and hemin detoxification were also modified. These data provide some answers on the cellular response to the iron-limitation stress that is important for the opportunistic behavior of this pathogen. |
| doi_str_mv | 10.1007/s00203-021-02342-2 |
| format | Article |
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Staphylococcus lugdunensis
have to cope with the condition of host-induced iron-limitation. Using the RNAseq approach, we performed the first global transcriptomic analysis of
S. lugdunensis
cells incubated in the absence and presence of iron chelator. One hundred and seventy-five genes were identified as members of the iron-limitation stimulon (127 up- and 48 downregulated). Six gene clusters known or likely required for the acquisition of iron have been identified. Among them, a novel Energy-Coupling Factor type transporter (ECF), homologous to the
lhaSTA
operon, has been found into a 13-gene putative operon and strongly overexpressed under iron-limitation condition. Moreover, the transcription of genes involved in resistance to oxidative stress (including catalase), virulence, transcriptional regulation, and hemin detoxification were also modified. These data provide some answers on the cellular response to the iron-limitation stress that is important for the opportunistic behavior of this pathogen.</description><identifier>ISSN: 0302-8933</identifier><identifier>EISSN: 1432-072X</identifier><identifier>DOI: 10.1007/s00203-021-02342-2</identifier><identifier>PMID: 33983488</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Biochemistry ; Biomedical and Life Sciences ; Biotechnology ; Catalase ; Cell Biology ; Detoxification ; Ecology ; Gene clusters ; Gene regulation ; Genes ; Hemin ; Homology ; Iron ; Life Sciences ; Microbial Ecology ; Microbiology ; Opportunist infection ; Oxidation resistance ; Oxidative stress ; Pathogens ; Short Communication ; Staphylococcus ; Transcription ; Transcriptomics ; Virulence</subject><ispartof>Archives of microbiology, 2021-08, Vol.203 (6), p.3687-3694</ispartof><rights>The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2021</rights><rights>The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2021.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c375t-14a73f73038f3369ae3fdf3f728b30c564dca7838397e17f8274e3a4324efedb3</citedby><cites>FETCH-LOGICAL-c375t-14a73f73038f3369ae3fdf3f728b30c564dca7838397e17f8274e3a4324efedb3</cites><orcidid>0000-0001-8588-2732</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00203-021-02342-2$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00203-021-02342-2$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33983488$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Aubourg, Marion</creatorcontrib><creatorcontrib>Gravey, François</creatorcontrib><creatorcontrib>Dhalluin, Anne</creatorcontrib><creatorcontrib>Giard, Jean-Christophe</creatorcontrib><title>Identification of the iron-limitation stimulon in Staphylococcus lugdunensis</title><title>Archives of microbiology</title><addtitle>Arch Microbiol</addtitle><addtitle>Arch Microbiol</addtitle><description>During the infectious process, pathogens such as
Staphylococcus lugdunensis
have to cope with the condition of host-induced iron-limitation. Using the RNAseq approach, we performed the first global transcriptomic analysis of
S. lugdunensis
cells incubated in the absence and presence of iron chelator. One hundred and seventy-five genes were identified as members of the iron-limitation stimulon (127 up- and 48 downregulated). Six gene clusters known or likely required for the acquisition of iron have been identified. Among them, a novel Energy-Coupling Factor type transporter (ECF), homologous to the
lhaSTA
operon, has been found into a 13-gene putative operon and strongly overexpressed under iron-limitation condition. Moreover, the transcription of genes involved in resistance to oxidative stress (including catalase), virulence, transcriptional regulation, and hemin detoxification were also modified. 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Staphylococcus lugdunensis
have to cope with the condition of host-induced iron-limitation. Using the RNAseq approach, we performed the first global transcriptomic analysis of
S. lugdunensis
cells incubated in the absence and presence of iron chelator. One hundred and seventy-five genes were identified as members of the iron-limitation stimulon (127 up- and 48 downregulated). Six gene clusters known or likely required for the acquisition of iron have been identified. Among them, a novel Energy-Coupling Factor type transporter (ECF), homologous to the
lhaSTA
operon, has been found into a 13-gene putative operon and strongly overexpressed under iron-limitation condition. Moreover, the transcription of genes involved in resistance to oxidative stress (including catalase), virulence, transcriptional regulation, and hemin detoxification were also modified. These data provide some answers on the cellular response to the iron-limitation stress that is important for the opportunistic behavior of this pathogen.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>33983488</pmid><doi>10.1007/s00203-021-02342-2</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0001-8588-2732</orcidid></addata></record> |
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| subjects | Biochemistry Biomedical and Life Sciences Biotechnology Catalase Cell Biology Detoxification Ecology Gene clusters Gene regulation Genes Hemin Homology Iron Life Sciences Microbial Ecology Microbiology Opportunist infection Oxidation resistance Oxidative stress Pathogens Short Communication Staphylococcus Transcription Transcriptomics Virulence |
| title | Identification of the iron-limitation stimulon in Staphylococcus lugdunensis |
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