Comparative study of IgG binding to megakaryocytes in immune and myelodysplastic thrombocytopenic patients
Immune thrombocytopenia (ITP) is a disorder in which autoantibodies are responsible for destruction and decreased production of platelets. In the meantime, thrombocytopenia is frequent in patients with myelodysplastic syndromes (MDS) and immune clearance of megakaryocytes could be a reason. The aim...
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| Veröffentlicht in: | Annals of hematology 2021-07, Vol.100 (7), p.1701-1709 |
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| container_title | Annals of hematology |
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| creator | Elzaeem, Doaa I. Sharkawi, Esmat A. El Zaki, Eman M. Ghobrial, Ayman G. El-Fatah, Aliaa S. Abd El-Hamed, Waleed M. Abd |
| description | Immune thrombocytopenia (ITP) is a disorder in which autoantibodies are responsible for destruction and decreased production of platelets. In the meantime, thrombocytopenia is frequent in patients with myelodysplastic syndromes (MDS) and immune clearance of megakaryocytes could be a reason. The aim of the present study is to evaluate and compare IgG binding to megakaryocytes in bone marrow of ITP and MDS patients to determine megakaryocytes targeting by autoantibodies in vivo as a mechanism of platelet underproduction in these disorders. The study was carried out on 20 ITP (group I) patients, 20 thrombocytopenic patients with (MDS) (group II), and 20 non-ITP patients as a control (group III) who were admitted to Minia University Hospital. Serial histological sections from bone marrow biopsies were stained for IgG. All patients in group I and 50% of group II patients showed bleeding tendency and the difference was significant (p |
| doi_str_mv | 10.1007/s00277-021-04556-2 |
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El ; Zaki, Eman M. ; Ghobrial, Ayman G. ; El-Fatah, Aliaa S. Abd ; El-Hamed, Waleed M. Abd</creator><creatorcontrib>Elzaeem, Doaa I. ; Sharkawi, Esmat A. El ; Zaki, Eman M. ; Ghobrial, Ayman G. ; El-Fatah, Aliaa S. Abd ; El-Hamed, Waleed M. Abd</creatorcontrib><description>Immune thrombocytopenia (ITP) is a disorder in which autoantibodies are responsible for destruction and decreased production of platelets. In the meantime, thrombocytopenia is frequent in patients with myelodysplastic syndromes (MDS) and immune clearance of megakaryocytes could be a reason. The aim of the present study is to evaluate and compare IgG binding to megakaryocytes in bone marrow of ITP and MDS patients to determine megakaryocytes targeting by autoantibodies in vivo as a mechanism of platelet underproduction in these disorders. The study was carried out on 20 ITP (group I) patients, 20 thrombocytopenic patients with (MDS) (group II), and 20 non-ITP patients as a control (group III) who were admitted to Minia University Hospital. Serial histological sections from bone marrow biopsies were stained for IgG. All patients in group I and 50% of group II patients showed bleeding tendency and the difference was significant (p < 0.001). No patient experienced fatigue in group I while 35% of patients in group II complained of easy fatigability, and the difference was significant (p < 0.008). High IgG antibody binding was found in ITP and MDS compared to the control group but no significant difference between ITP and MDS patients (14/20 (70%) vs. 13/20 (65%)) (p value = 0.736). Antibody binding to megakaryocytes in a proportion of MDS patients suggests that immune-mediated mechanism underlies platelet underproduction in those patients.</description><identifier>ISSN: 0939-5555</identifier><identifier>EISSN: 1432-0584</identifier><identifier>DOI: 10.1007/s00277-021-04556-2</identifier><identifier>PMID: 33982136</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Bone marrow ; Hematology ; Medicine ; Medicine & Public Health ; Myelodysplastic syndromes ; Oncology ; Original Article ; Thrombocytopenia</subject><ispartof>Annals of hematology, 2021-07, Vol.100 (7), p.1701-1709</ispartof><rights>The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2021</rights><rights>The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2021.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c375t-31668d4eeb27fbc93d2f0939ed26546a6225bd9395bed879007313b3335e0ea93</citedby><cites>FETCH-LOGICAL-c375t-31668d4eeb27fbc93d2f0939ed26546a6225bd9395bed879007313b3335e0ea93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00277-021-04556-2$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00277-021-04556-2$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33982136$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Elzaeem, Doaa I.</creatorcontrib><creatorcontrib>Sharkawi, Esmat A. 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The aim of the present study is to evaluate and compare IgG binding to megakaryocytes in bone marrow of ITP and MDS patients to determine megakaryocytes targeting by autoantibodies in vivo as a mechanism of platelet underproduction in these disorders. The study was carried out on 20 ITP (group I) patients, 20 thrombocytopenic patients with (MDS) (group II), and 20 non-ITP patients as a control (group III) who were admitted to Minia University Hospital. Serial histological sections from bone marrow biopsies were stained for IgG. All patients in group I and 50% of group II patients showed bleeding tendency and the difference was significant (p < 0.001). No patient experienced fatigue in group I while 35% of patients in group II complained of easy fatigability, and the difference was significant (p < 0.008). High IgG antibody binding was found in ITP and MDS compared to the control group but no significant difference between ITP and MDS patients (14/20 (70%) vs. 13/20 (65%)) (p value = 0.736). Antibody binding to megakaryocytes in a proportion of MDS patients suggests that immune-mediated mechanism underlies platelet underproduction in those patients.</description><subject>Bone marrow</subject><subject>Hematology</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Myelodysplastic syndromes</subject><subject>Oncology</subject><subject>Original Article</subject><subject>Thrombocytopenia</subject><issn>0939-5555</issn><issn>1432-0584</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNp9kU9v3CAQxVGVqNmk_QI9VEi59OIEGOM_x2qVJpEi5ZKeETbjrbcGXMCR_O3DdtNWyqFzGWn4zQPeI-QTZ1ecsfo6MibqumCCF6yUsirEO7LhJYiCyaY8IRvWQlvIXGfkPMY9Y1w0pXhPzgDaRnCoNmS_9XbWQafxGWlMi1mpH-j97pZ2ozOj29HkqcWd_qnD6vs1YaSjo6O1i0OqnaF2xcmbNc6TjmnsafoRvO0OqJ_R5cGcxdGl-IGcDnqK-PG1X5Dv326etnfFw-Pt_fbrQ9FDLVMBvKoaUyJ2oh66vgUjhsNH0IhKlpWuhJCdyQPZoWnqNjsBHDoAkMhQt3BBvhx15-B_LRiTsmPscZq0Q79EJaSogMkSWEYv36B7vwSXX5epkmXDai4yJY5UH3yMAQc1h9FmPxRn6pCEOiahchLqdxLqsPT5VXrpLJq_K3-szwAcgZiP3A7Dv7v_I_sCbHWUdA</recordid><startdate>20210701</startdate><enddate>20210701</enddate><creator>Elzaeem, Doaa I.</creator><creator>Sharkawi, Esmat A. 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| subjects | Bone marrow Hematology Medicine Medicine & Public Health Myelodysplastic syndromes Oncology Original Article Thrombocytopenia |
| title | Comparative study of IgG binding to megakaryocytes in immune and myelodysplastic thrombocytopenic patients |
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