C‐C chemokine hepatocellular carcinoma motif ligand 5‐deficiency promotes hepatocellular carcinoma progression by affecting B cell recruitment
Objective To evaluate the expression of C‐C motif chemokine ligand 5 (CCL5) in hepatocellular carcinoma (HCC) and to explore its role in regulating the immune microenvironment and the related mechanism in tumor immunity. Methods The mRNA expression level of CCL5 in HCC and adjacent non‐cancerous tis...
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| Veröffentlicht in: | Journal of digestive diseases 2021-07, Vol.22 (7), p.433-441 |
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| creator | Li, Xiang Han, Qiu Cheng Yu, Chang Luo, Yi Chun Wang, Fang Sun, Xue Hua Gao, Yue Qiu Tan, Wei Feng Xia, Qiang |
| description | Objective
To evaluate the expression of C‐C motif chemokine ligand 5 (CCL5) in hepatocellular carcinoma (HCC) and to explore its role in regulating the immune microenvironment and the related mechanism in tumor immunity.
Methods
The mRNA expression level of CCL5 in HCC and adjacent non‐cancerous tissues was measured by quantitative polymerase chain reaction and the protein expression was examined by immunohistochemistry. Serum CCL5 expression was measured by an enzyme‐linked immunosorbent assay (ELISA). C57BL/6 wild‐type (WT) and Ccl5‐knockout (Ccl5−/−) mice were utilized to conduct the diethylnitrosamine‐induced HCC model. The immune cell population was determined by flow cytometry, and peripheral serum immunoglobulin M (IgM) level was quantified by ELISA.
Results
CCL5 expression was low in HCC tissue and peripheral blood compared with adjacent non‐cancerous tissues or controls, and its expression was correlated with the overall survival, cancer recurrence and distant metastasis. In the HCC mouse model, liver‐to‐body weight ratio was of the Ccl5−/− group were higher than that of the WT group. Moreover, compared with the WT mice, the number of B cells in the tumor tissue of the Ccl5−/− mice was lower, while there were no significant differences in the other immune cell populations. Furthermore, serum IgM level of the Ccl5−/− mice was significantly lower than that of the WT mice.
Conclusion
CCL5 expression is decreased in HCC tissues. CCL5 deficiency reduces B cell recruitment and decreases IgM secretion in HCC, potentially leading to tumor progression.
CCL5 expression was low in hepatocellular carcinoma (HCC) tissue and peripheral blood of the HCC patients, and its expression was correlated with the overall survival, recurrence and metastasis of the tumor. Deficiency of CCL5 in hepatocellular carcinoma accelerates tumor progression by decreasing B‐cell recruitment and IgM secretion. |
| doi_str_mv | 10.1111/1751-2980.12997 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2526142330</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2550173046</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3487-2fea6edce7f3866bc8df1adebe5d765a07a34546d137d81d03a2577f8c2afebd3</originalsourceid><addsrcrecordid>eNqFkctqGzEUhofSQtOk624F3XTjRJeRNF4249zAkE26FrJ0ZCudkRxphuJdHiHkEfMk0dTBi0KJNrp93-GHv6q-EXxKyjojkpMZnTflSudz-aE6Orx8PJwl_Vx9yfkeYy5kI46q5_bl8alFZgN9_O0DoA1s9RANdN3Y6YSMTsaH2GvUx8E71Pm1DhbxYllw3ngIZoe2KZZvyP-3C7FOkLOPAa12SDsHZvBhjc7RRKMEJo1-6CEMJ9Unp7sMX9_24-rX5cVdez1b3l7dtD-XM8PqRs6oAy3AGpCONUKsTGMd0RZWwK0UXGOpWc1rYQmTtiEWM025lK4xVDtYWXZc_djPLdkeRsiD6n2ewugAccyKcipITRnDBf3-D3ofxxRKukJxTCTDtSjU2Z4yKeacwKlt8r1OO0WwmjpSUwtqakT97agYfG_88R3s3sNVu1jsvVf56pj7</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2550173046</pqid></control><display><type>article</type><title>C‐C chemokine hepatocellular carcinoma motif ligand 5‐deficiency promotes hepatocellular carcinoma progression by affecting B cell recruitment</title><source>Access via Wiley Online Library</source><creator>Li, Xiang ; Han, Qiu Cheng ; Yu, Chang ; Luo, Yi Chun ; Wang, Fang ; Sun, Xue Hua ; Gao, Yue Qiu ; Tan, Wei Feng ; Xia, Qiang</creator><creatorcontrib>Li, Xiang ; Han, Qiu Cheng ; Yu, Chang ; Luo, Yi Chun ; Wang, Fang ; Sun, Xue Hua ; Gao, Yue Qiu ; Tan, Wei Feng ; Xia, Qiang</creatorcontrib><description>Objective
To evaluate the expression of C‐C motif chemokine ligand 5 (CCL5) in hepatocellular carcinoma (HCC) and to explore its role in regulating the immune microenvironment and the related mechanism in tumor immunity.
Methods
The mRNA expression level of CCL5 in HCC and adjacent non‐cancerous tissues was measured by quantitative polymerase chain reaction and the protein expression was examined by immunohistochemistry. Serum CCL5 expression was measured by an enzyme‐linked immunosorbent assay (ELISA). C57BL/6 wild‐type (WT) and Ccl5‐knockout (Ccl5−/−) mice were utilized to conduct the diethylnitrosamine‐induced HCC model. The immune cell population was determined by flow cytometry, and peripheral serum immunoglobulin M (IgM) level was quantified by ELISA.
Results
CCL5 expression was low in HCC tissue and peripheral blood compared with adjacent non‐cancerous tissues or controls, and its expression was correlated with the overall survival, cancer recurrence and distant metastasis. In the HCC mouse model, liver‐to‐body weight ratio was of the Ccl5−/− group were higher than that of the WT group. Moreover, compared with the WT mice, the number of B cells in the tumor tissue of the Ccl5−/− mice was lower, while there were no significant differences in the other immune cell populations. Furthermore, serum IgM level of the Ccl5−/− mice was significantly lower than that of the WT mice.
Conclusion
CCL5 expression is decreased in HCC tissues. CCL5 deficiency reduces B cell recruitment and decreases IgM secretion in HCC, potentially leading to tumor progression.
CCL5 expression was low in hepatocellular carcinoma (HCC) tissue and peripheral blood of the HCC patients, and its expression was correlated with the overall survival, recurrence and metastasis of the tumor. Deficiency of CCL5 in hepatocellular carcinoma accelerates tumor progression by decreasing B‐cell recruitment and IgM secretion.</description><identifier>ISSN: 1751-2972</identifier><identifier>EISSN: 1751-2980</identifier><identifier>DOI: 10.1111/1751-2980.12997</identifier><language>eng</language><publisher>Melbourne: Wiley Publishing Asia Pty Ltd</publisher><subject>B cell recruitment ; Body weight ; chemokine CCL5 ; Chemokines ; Diethylnitrosamine ; disease progression ; Enzyme-linked immunosorbent assay ; Flow cytometry ; Gene expression ; Hepatocellular carcinoma ; hepatocellular carcinomaimmunoglobulin M ; Immunoglobulin M ; Immunohistochemistry ; Ligands ; Liver cancer ; Lymphocytes B ; Metastases ; Microenvironments ; Peripheral blood ; Polymerase chain reaction</subject><ispartof>Journal of digestive diseases, 2021-07, Vol.22 (7), p.433-441</ispartof><rights>2021 Chinese Medical Association Shanghai Branch, Chinese Society of Gastroenterology, Renji Hospital Affiliated to Shanghai Jiaotong University School of Medicine and John Wiley & Sons Australia, Ltd.</rights><rights>2021 Chinese Medical Association Shanghai Branch, Chinese Society of Gastroenterology, Renji Hospital Affiliated to Shanghai Jiaotong University School of Medicine and John Wiley & Sons Australia, Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3487-2fea6edce7f3866bc8df1adebe5d765a07a34546d137d81d03a2577f8c2afebd3</citedby><cites>FETCH-LOGICAL-c3487-2fea6edce7f3866bc8df1adebe5d765a07a34546d137d81d03a2577f8c2afebd3</cites><orcidid>0000-0001-7644-1994</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2F1751-2980.12997$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2F1751-2980.12997$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids></links><search><creatorcontrib>Li, Xiang</creatorcontrib><creatorcontrib>Han, Qiu Cheng</creatorcontrib><creatorcontrib>Yu, Chang</creatorcontrib><creatorcontrib>Luo, Yi Chun</creatorcontrib><creatorcontrib>Wang, Fang</creatorcontrib><creatorcontrib>Sun, Xue Hua</creatorcontrib><creatorcontrib>Gao, Yue Qiu</creatorcontrib><creatorcontrib>Tan, Wei Feng</creatorcontrib><creatorcontrib>Xia, Qiang</creatorcontrib><title>C‐C chemokine hepatocellular carcinoma motif ligand 5‐deficiency promotes hepatocellular carcinoma progression by affecting B cell recruitment</title><title>Journal of digestive diseases</title><description>Objective
To evaluate the expression of C‐C motif chemokine ligand 5 (CCL5) in hepatocellular carcinoma (HCC) and to explore its role in regulating the immune microenvironment and the related mechanism in tumor immunity.
Methods
The mRNA expression level of CCL5 in HCC and adjacent non‐cancerous tissues was measured by quantitative polymerase chain reaction and the protein expression was examined by immunohistochemistry. Serum CCL5 expression was measured by an enzyme‐linked immunosorbent assay (ELISA). C57BL/6 wild‐type (WT) and Ccl5‐knockout (Ccl5−/−) mice were utilized to conduct the diethylnitrosamine‐induced HCC model. The immune cell population was determined by flow cytometry, and peripheral serum immunoglobulin M (IgM) level was quantified by ELISA.
Results
CCL5 expression was low in HCC tissue and peripheral blood compared with adjacent non‐cancerous tissues or controls, and its expression was correlated with the overall survival, cancer recurrence and distant metastasis. In the HCC mouse model, liver‐to‐body weight ratio was of the Ccl5−/− group were higher than that of the WT group. Moreover, compared with the WT mice, the number of B cells in the tumor tissue of the Ccl5−/− mice was lower, while there were no significant differences in the other immune cell populations. Furthermore, serum IgM level of the Ccl5−/− mice was significantly lower than that of the WT mice.
Conclusion
CCL5 expression is decreased in HCC tissues. CCL5 deficiency reduces B cell recruitment and decreases IgM secretion in HCC, potentially leading to tumor progression.
CCL5 expression was low in hepatocellular carcinoma (HCC) tissue and peripheral blood of the HCC patients, and its expression was correlated with the overall survival, recurrence and metastasis of the tumor. Deficiency of CCL5 in hepatocellular carcinoma accelerates tumor progression by decreasing B‐cell recruitment and IgM secretion.</description><subject>B cell recruitment</subject><subject>Body weight</subject><subject>chemokine CCL5</subject><subject>Chemokines</subject><subject>Diethylnitrosamine</subject><subject>disease progression</subject><subject>Enzyme-linked immunosorbent assay</subject><subject>Flow cytometry</subject><subject>Gene expression</subject><subject>Hepatocellular carcinoma</subject><subject>hepatocellular carcinomaimmunoglobulin M</subject><subject>Immunoglobulin M</subject><subject>Immunohistochemistry</subject><subject>Ligands</subject><subject>Liver cancer</subject><subject>Lymphocytes B</subject><subject>Metastases</subject><subject>Microenvironments</subject><subject>Peripheral blood</subject><subject>Polymerase chain reaction</subject><issn>1751-2972</issn><issn>1751-2980</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNqFkctqGzEUhofSQtOk624F3XTjRJeRNF4249zAkE26FrJ0ZCudkRxphuJdHiHkEfMk0dTBi0KJNrp93-GHv6q-EXxKyjojkpMZnTflSudz-aE6Orx8PJwl_Vx9yfkeYy5kI46q5_bl8alFZgN9_O0DoA1s9RANdN3Y6YSMTsaH2GvUx8E71Pm1DhbxYllw3ngIZoe2KZZvyP-3C7FOkLOPAa12SDsHZvBhjc7RRKMEJo1-6CEMJ9Unp7sMX9_24-rX5cVdez1b3l7dtD-XM8PqRs6oAy3AGpCONUKsTGMd0RZWwK0UXGOpWc1rYQmTtiEWM025lK4xVDtYWXZc_djPLdkeRsiD6n2ewugAccyKcipITRnDBf3-D3ofxxRKukJxTCTDtSjU2Z4yKeacwKlt8r1OO0WwmjpSUwtqakT97agYfG_88R3s3sNVu1jsvVf56pj7</recordid><startdate>202107</startdate><enddate>202107</enddate><creator>Li, Xiang</creator><creator>Han, Qiu Cheng</creator><creator>Yu, Chang</creator><creator>Luo, Yi Chun</creator><creator>Wang, Fang</creator><creator>Sun, Xue Hua</creator><creator>Gao, Yue Qiu</creator><creator>Tan, Wei Feng</creator><creator>Xia, Qiang</creator><general>Wiley Publishing Asia Pty Ltd</general><general>Wiley Subscription Services, Inc</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>K9.</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-7644-1994</orcidid></search><sort><creationdate>202107</creationdate><title>C‐C chemokine hepatocellular carcinoma motif ligand 5‐deficiency promotes hepatocellular carcinoma progression by affecting B cell recruitment</title><author>Li, Xiang ; Han, Qiu Cheng ; Yu, Chang ; Luo, Yi Chun ; Wang, Fang ; Sun, Xue Hua ; Gao, Yue Qiu ; Tan, Wei Feng ; Xia, Qiang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3487-2fea6edce7f3866bc8df1adebe5d765a07a34546d137d81d03a2577f8c2afebd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>B cell recruitment</topic><topic>Body weight</topic><topic>chemokine CCL5</topic><topic>Chemokines</topic><topic>Diethylnitrosamine</topic><topic>disease progression</topic><topic>Enzyme-linked immunosorbent assay</topic><topic>Flow cytometry</topic><topic>Gene expression</topic><topic>Hepatocellular carcinoma</topic><topic>hepatocellular carcinomaimmunoglobulin M</topic><topic>Immunoglobulin M</topic><topic>Immunohistochemistry</topic><topic>Ligands</topic><topic>Liver cancer</topic><topic>Lymphocytes B</topic><topic>Metastases</topic><topic>Microenvironments</topic><topic>Peripheral blood</topic><topic>Polymerase chain reaction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Xiang</creatorcontrib><creatorcontrib>Han, Qiu Cheng</creatorcontrib><creatorcontrib>Yu, Chang</creatorcontrib><creatorcontrib>Luo, Yi Chun</creatorcontrib><creatorcontrib>Wang, Fang</creatorcontrib><creatorcontrib>Sun, Xue Hua</creatorcontrib><creatorcontrib>Gao, Yue Qiu</creatorcontrib><creatorcontrib>Tan, Wei Feng</creatorcontrib><creatorcontrib>Xia, Qiang</creatorcontrib><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of digestive diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Xiang</au><au>Han, Qiu Cheng</au><au>Yu, Chang</au><au>Luo, Yi Chun</au><au>Wang, Fang</au><au>Sun, Xue Hua</au><au>Gao, Yue Qiu</au><au>Tan, Wei Feng</au><au>Xia, Qiang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>C‐C chemokine hepatocellular carcinoma motif ligand 5‐deficiency promotes hepatocellular carcinoma progression by affecting B cell recruitment</atitle><jtitle>Journal of digestive diseases</jtitle><date>2021-07</date><risdate>2021</risdate><volume>22</volume><issue>7</issue><spage>433</spage><epage>441</epage><pages>433-441</pages><issn>1751-2972</issn><eissn>1751-2980</eissn><abstract>Objective
To evaluate the expression of C‐C motif chemokine ligand 5 (CCL5) in hepatocellular carcinoma (HCC) and to explore its role in regulating the immune microenvironment and the related mechanism in tumor immunity.
Methods
The mRNA expression level of CCL5 in HCC and adjacent non‐cancerous tissues was measured by quantitative polymerase chain reaction and the protein expression was examined by immunohistochemistry. Serum CCL5 expression was measured by an enzyme‐linked immunosorbent assay (ELISA). C57BL/6 wild‐type (WT) and Ccl5‐knockout (Ccl5−/−) mice were utilized to conduct the diethylnitrosamine‐induced HCC model. The immune cell population was determined by flow cytometry, and peripheral serum immunoglobulin M (IgM) level was quantified by ELISA.
Results
CCL5 expression was low in HCC tissue and peripheral blood compared with adjacent non‐cancerous tissues or controls, and its expression was correlated with the overall survival, cancer recurrence and distant metastasis. In the HCC mouse model, liver‐to‐body weight ratio was of the Ccl5−/− group were higher than that of the WT group. Moreover, compared with the WT mice, the number of B cells in the tumor tissue of the Ccl5−/− mice was lower, while there were no significant differences in the other immune cell populations. Furthermore, serum IgM level of the Ccl5−/− mice was significantly lower than that of the WT mice.
Conclusion
CCL5 expression is decreased in HCC tissues. CCL5 deficiency reduces B cell recruitment and decreases IgM secretion in HCC, potentially leading to tumor progression.
CCL5 expression was low in hepatocellular carcinoma (HCC) tissue and peripheral blood of the HCC patients, and its expression was correlated with the overall survival, recurrence and metastasis of the tumor. Deficiency of CCL5 in hepatocellular carcinoma accelerates tumor progression by decreasing B‐cell recruitment and IgM secretion.</abstract><cop>Melbourne</cop><pub>Wiley Publishing Asia Pty Ltd</pub><doi>10.1111/1751-2980.12997</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0001-7644-1994</orcidid></addata></record> |
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| ispartof | Journal of digestive diseases, 2021-07, Vol.22 (7), p.433-441 |
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| subjects | B cell recruitment Body weight chemokine CCL5 Chemokines Diethylnitrosamine disease progression Enzyme-linked immunosorbent assay Flow cytometry Gene expression Hepatocellular carcinoma hepatocellular carcinomaimmunoglobulin M Immunoglobulin M Immunohistochemistry Ligands Liver cancer Lymphocytes B Metastases Microenvironments Peripheral blood Polymerase chain reaction |
| title | C‐C chemokine hepatocellular carcinoma motif ligand 5‐deficiency promotes hepatocellular carcinoma progression by affecting B cell recruitment |
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