Race-specific changes in endothelial inflammation and microRNA in response to an acute inflammatory stimulus
Both aberrant vascular reactivity to acute cardiovascular stress and epigenetic mechanisms such as microRNA (miR) may underlie the increased propensity for African Americans (AA) to develop cardiovascular disease. This study assessed racial differences in acute induced endothelial inflammation and r...
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| Veröffentlicht in: | American journal of physiology. Heart and circulatory physiology 2021-06, Vol.320 (6), p.H2371-H2384 |
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| container_title | American journal of physiology. Heart and circulatory physiology |
| container_volume | 320 |
| creator | Sapp, Ryan M Chesney, Catalina A Springer, Catherine B Laskowski, Matthew R Singer, Daniel B Eagan, Lauren E Mascone, Sara E Evans, William S Prior, Steven J Hagberg, James M Ranadive, Sushant M |
| description | Both aberrant vascular reactivity to acute cardiovascular stress and epigenetic mechanisms such as microRNA (miR) may underlie the increased propensity for African Americans (AA) to develop cardiovascular disease. This study assessed racial differences in acute induced endothelial inflammation and related miRs. Cultured human umbilical vein endothelial cells (HUVECs) derived from AA and Caucasian Americans (CA) were exposed to the influenza vaccine to determine changes in inflammatory markers, endothelial nitric oxide synthase (eNOS), and miR expression/release. Endothelial function (flow-mediated dilation [FMD]), circulating IL-6, and circulating miR were also measured in young, healthy AA and CA individuals before and after receiving the influenza vaccine. There were no significant racial differences in any parameters at baseline. The vaccine induced increases in IL-6 release (24%, P=0.02) and ICAM-1 mRNA (40%, P=0.03), as well as reduced eNOS mRNA (24%, P=0.04) in AA HUVECs, but not in CA HUVECs (all P>0.05). Intracellular levels of anti-inflammatory miR-221-3p and miR-222-3p increased specifically in CA HUVECs (72% and 53%, p=0.04 and p=0.06), while others did not change in either race. HUVEC secretion of several miRs decreased in both races, while the release of anti-inflammatory miR-150-5p was decreased only by AA cells (-30%, P=0.03). In individuals of both races, circulating IL-6 increased ~two-fold 24 hours after vaccination (both P |
| doi_str_mv | 10.1152/AJPHEART.00991.2020 |
| format | Article |
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This study assessed racial differences in acute induced endothelial inflammation and related miRs. Cultured human umbilical vein endothelial cells (HUVECs) derived from AA and Caucasian Americans (CA) were exposed to the influenza vaccine to determine changes in inflammatory markers, endothelial nitric oxide synthase (eNOS), and miR expression/release. Endothelial function (flow-mediated dilation [FMD]), circulating IL-6, and circulating miR were also measured in young, healthy AA and CA individuals before and after receiving the influenza vaccine. There were no significant racial differences in any parameters at baseline. The vaccine induced increases in IL-6 release (24%, P=0.02) and ICAM-1 mRNA (40%, P=0.03), as well as reduced eNOS mRNA (24%, P=0.04) in AA HUVECs, but not in CA HUVECs (all P>0.05). Intracellular levels of anti-inflammatory miR-221-3p and miR-222-3p increased specifically in CA HUVECs (72% and 53%, p=0.04 and p=0.06), while others did not change in either race. HUVEC secretion of several miRs decreased in both races, while the release of anti-inflammatory miR-150-5p was decreased only by AA cells (-30%, P=0.03). In individuals of both races, circulating IL-6 increased ~two-fold 24 hours after vaccination (both P<0.01) and returned to baseline levels by 48 hours, while FMD remained unchanged. Although macrovascular function was unaffected by acute inflammation in AA and CA individuals, AA endothelial cells exhibited increased susceptibility to acute inflammation and unique changes in related miR.</description><identifier>ISSN: 0363-6135</identifier><identifier>EISSN: 1522-1539</identifier><identifier>DOI: 10.1152/AJPHEART.00991.2020</identifier><identifier>PMID: 33961505</identifier><language>eng</language><publisher>United States: American Physiological Society</publisher><subject>Cardiovascular diseases ; Endothelial cells ; Epigenetics ; Inflammation ; Influenza ; Intercellular adhesion molecule 1 ; Interleukin 6 ; Intracellular levels ; MicroRNAs ; Minority & ethnic groups ; miRNA ; mRNA ; Nitric oxide ; Nitric-oxide synthase ; Race ; Races ; Racial differences ; Ribonucleic acid ; RNA ; Umbilical vein ; Vaccination ; Vaccines</subject><ispartof>American journal of physiology. Heart and circulatory physiology, 2021-06, Vol.320 (6), p.H2371-H2384</ispartof><rights>Copyright American Physiological Society Jun 2021</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c333t-fe69c0ff5a767571ee599dbc8e58bb6fb777822371ecad58214b1e8c8d84138f3</citedby><cites>FETCH-LOGICAL-c333t-fe69c0ff5a767571ee599dbc8e58bb6fb777822371ecad58214b1e8c8d84138f3</cites><orcidid>0000-0002-2845-4544 ; 0000-0003-3769-4723 ; 0000-0002-4627-6489 ; 0000-0001-7598-3679</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,3039,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33961505$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sapp, Ryan M</creatorcontrib><creatorcontrib>Chesney, Catalina A</creatorcontrib><creatorcontrib>Springer, Catherine B</creatorcontrib><creatorcontrib>Laskowski, Matthew R</creatorcontrib><creatorcontrib>Singer, Daniel B</creatorcontrib><creatorcontrib>Eagan, Lauren E</creatorcontrib><creatorcontrib>Mascone, Sara E</creatorcontrib><creatorcontrib>Evans, William S</creatorcontrib><creatorcontrib>Prior, Steven J</creatorcontrib><creatorcontrib>Hagberg, James M</creatorcontrib><creatorcontrib>Ranadive, Sushant M</creatorcontrib><title>Race-specific changes in endothelial inflammation and microRNA in response to an acute inflammatory stimulus</title><title>American journal of physiology. Heart and circulatory physiology</title><addtitle>Am J Physiol Heart Circ Physiol</addtitle><description>Both aberrant vascular reactivity to acute cardiovascular stress and epigenetic mechanisms such as microRNA (miR) may underlie the increased propensity for African Americans (AA) to develop cardiovascular disease. This study assessed racial differences in acute induced endothelial inflammation and related miRs. Cultured human umbilical vein endothelial cells (HUVECs) derived from AA and Caucasian Americans (CA) were exposed to the influenza vaccine to determine changes in inflammatory markers, endothelial nitric oxide synthase (eNOS), and miR expression/release. Endothelial function (flow-mediated dilation [FMD]), circulating IL-6, and circulating miR were also measured in young, healthy AA and CA individuals before and after receiving the influenza vaccine. There were no significant racial differences in any parameters at baseline. The vaccine induced increases in IL-6 release (24%, P=0.02) and ICAM-1 mRNA (40%, P=0.03), as well as reduced eNOS mRNA (24%, P=0.04) in AA HUVECs, but not in CA HUVECs (all P>0.05). Intracellular levels of anti-inflammatory miR-221-3p and miR-222-3p increased specifically in CA HUVECs (72% and 53%, p=0.04 and p=0.06), while others did not change in either race. HUVEC secretion of several miRs decreased in both races, while the release of anti-inflammatory miR-150-5p was decreased only by AA cells (-30%, P=0.03). In individuals of both races, circulating IL-6 increased ~two-fold 24 hours after vaccination (both P<0.01) and returned to baseline levels by 48 hours, while FMD remained unchanged. Although macrovascular function was unaffected by acute inflammation in AA and CA individuals, AA endothelial cells exhibited increased susceptibility to acute inflammation and unique changes in related miR.</description><subject>Cardiovascular diseases</subject><subject>Endothelial cells</subject><subject>Epigenetics</subject><subject>Inflammation</subject><subject>Influenza</subject><subject>Intercellular adhesion molecule 1</subject><subject>Interleukin 6</subject><subject>Intracellular levels</subject><subject>MicroRNAs</subject><subject>Minority & ethnic groups</subject><subject>miRNA</subject><subject>mRNA</subject><subject>Nitric oxide</subject><subject>Nitric-oxide synthase</subject><subject>Race</subject><subject>Races</subject><subject>Racial differences</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>Umbilical vein</subject><subject>Vaccination</subject><subject>Vaccines</subject><issn>0363-6135</issn><issn>1522-1539</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNpdkU1r3DAQhkVJaTab_oJAMeTSi7eSZmVJxyWk-SC0YUnPRpZHXS225Ur2If8-2nw0kNMwvM87zMxLyBmjK8YE_7G5vb--3GwfVpRqzVaccvqJLLLCSyZAH5EFhQrKioE4Jicp7SmlQlbwhRwD6IoJKhak2xqLZRrReudtYXdm-Iup8EOBQxumHXbedLl1nel7M_kwFGZoi97bGLa_NgcwYhrDkLCYQtYKY-cJ3x0hPhZp8v3czemUfHamS_j1tS7Jn5-XDxfX5d3vq5uLzV1pAWAqHVbaUueEkZUUkiEKrdvGKhSqaSrXSCkV55AVa1qhOFs3DJVVrVozUA6W5PvL3DGGfzOmqe59sth1ZsAwp5oLvoaKasUyev4B3Yc5Dnm7TIHWIGWuSwIvVL46pYiuHqPvTXysGa0PYdRmP-7QxKl-DqM-hJFd315nz02P7X_P2_fhCVkHh3w</recordid><startdate>20210601</startdate><enddate>20210601</enddate><creator>Sapp, Ryan M</creator><creator>Chesney, Catalina A</creator><creator>Springer, Catherine B</creator><creator>Laskowski, Matthew R</creator><creator>Singer, Daniel B</creator><creator>Eagan, Lauren E</creator><creator>Mascone, Sara E</creator><creator>Evans, William S</creator><creator>Prior, Steven J</creator><creator>Hagberg, James M</creator><creator>Ranadive, Sushant M</creator><general>American Physiological Society</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7QR</scope><scope>7TS</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-2845-4544</orcidid><orcidid>https://orcid.org/0000-0003-3769-4723</orcidid><orcidid>https://orcid.org/0000-0002-4627-6489</orcidid><orcidid>https://orcid.org/0000-0001-7598-3679</orcidid></search><sort><creationdate>20210601</creationdate><title>Race-specific changes in endothelial inflammation and microRNA in response to an acute inflammatory stimulus</title><author>Sapp, Ryan M ; Chesney, Catalina A ; Springer, Catherine B ; Laskowski, Matthew R ; Singer, Daniel B ; Eagan, Lauren E ; Mascone, Sara E ; Evans, William S ; Prior, Steven J ; Hagberg, James M ; Ranadive, Sushant M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c333t-fe69c0ff5a767571ee599dbc8e58bb6fb777822371ecad58214b1e8c8d84138f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Cardiovascular diseases</topic><topic>Endothelial cells</topic><topic>Epigenetics</topic><topic>Inflammation</topic><topic>Influenza</topic><topic>Intercellular adhesion molecule 1</topic><topic>Interleukin 6</topic><topic>Intracellular levels</topic><topic>MicroRNAs</topic><topic>Minority & ethnic groups</topic><topic>miRNA</topic><topic>mRNA</topic><topic>Nitric oxide</topic><topic>Nitric-oxide synthase</topic><topic>Race</topic><topic>Races</topic><topic>Racial differences</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>Umbilical vein</topic><topic>Vaccination</topic><topic>Vaccines</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sapp, Ryan M</creatorcontrib><creatorcontrib>Chesney, Catalina A</creatorcontrib><creatorcontrib>Springer, Catherine B</creatorcontrib><creatorcontrib>Laskowski, Matthew R</creatorcontrib><creatorcontrib>Singer, Daniel B</creatorcontrib><creatorcontrib>Eagan, Lauren E</creatorcontrib><creatorcontrib>Mascone, Sara E</creatorcontrib><creatorcontrib>Evans, William S</creatorcontrib><creatorcontrib>Prior, Steven J</creatorcontrib><creatorcontrib>Hagberg, James M</creatorcontrib><creatorcontrib>Ranadive, Sushant M</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Physical Education Index</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>American journal of physiology. 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Heart and circulatory physiology</jtitle><addtitle>Am J Physiol Heart Circ Physiol</addtitle><date>2021-06-01</date><risdate>2021</risdate><volume>320</volume><issue>6</issue><spage>H2371</spage><epage>H2384</epage><pages>H2371-H2384</pages><issn>0363-6135</issn><eissn>1522-1539</eissn><abstract>Both aberrant vascular reactivity to acute cardiovascular stress and epigenetic mechanisms such as microRNA (miR) may underlie the increased propensity for African Americans (AA) to develop cardiovascular disease. This study assessed racial differences in acute induced endothelial inflammation and related miRs. Cultured human umbilical vein endothelial cells (HUVECs) derived from AA and Caucasian Americans (CA) were exposed to the influenza vaccine to determine changes in inflammatory markers, endothelial nitric oxide synthase (eNOS), and miR expression/release. Endothelial function (flow-mediated dilation [FMD]), circulating IL-6, and circulating miR were also measured in young, healthy AA and CA individuals before and after receiving the influenza vaccine. There were no significant racial differences in any parameters at baseline. The vaccine induced increases in IL-6 release (24%, P=0.02) and ICAM-1 mRNA (40%, P=0.03), as well as reduced eNOS mRNA (24%, P=0.04) in AA HUVECs, but not in CA HUVECs (all P>0.05). Intracellular levels of anti-inflammatory miR-221-3p and miR-222-3p increased specifically in CA HUVECs (72% and 53%, p=0.04 and p=0.06), while others did not change in either race. HUVEC secretion of several miRs decreased in both races, while the release of anti-inflammatory miR-150-5p was decreased only by AA cells (-30%, P=0.03). In individuals of both races, circulating IL-6 increased ~two-fold 24 hours after vaccination (both P<0.01) and returned to baseline levels by 48 hours, while FMD remained unchanged. Although macrovascular function was unaffected by acute inflammation in AA and CA individuals, AA endothelial cells exhibited increased susceptibility to acute inflammation and unique changes in related miR.</abstract><cop>United States</cop><pub>American Physiological Society</pub><pmid>33961505</pmid><doi>10.1152/AJPHEART.00991.2020</doi><orcidid>https://orcid.org/0000-0002-2845-4544</orcidid><orcidid>https://orcid.org/0000-0003-3769-4723</orcidid><orcidid>https://orcid.org/0000-0002-4627-6489</orcidid><orcidid>https://orcid.org/0000-0001-7598-3679</orcidid></addata></record> |
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| source | American Physiological Society; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Cardiovascular diseases Endothelial cells Epigenetics Inflammation Influenza Intercellular adhesion molecule 1 Interleukin 6 Intracellular levels MicroRNAs Minority & ethnic groups miRNA mRNA Nitric oxide Nitric-oxide synthase Race Races Racial differences Ribonucleic acid RNA Umbilical vein Vaccination Vaccines |
| title | Race-specific changes in endothelial inflammation and microRNA in response to an acute inflammatory stimulus |
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