Design of experiments driven optimization of alkaline pretreatment and saccharification for sugarcane bagasse

•Simultaneous optimisation of pretreatment and saccharification using design tool.•High-resolution Design of Experiments maximizes sugar release in sugarcane bagasse.•Fractional Factorial Design was combined with a Central Composite Orthogonal design.•Design of Experiments monitored by compositional...

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Veröffentlicht in:Bioresource technology 2021-02, Vol.321, p.124499-124499, Article 124499
Hauptverfasser: Mota, Thatiane R., Oliveira, Dyoni M., Simister, Rachael, Whitehead, Caragh, Lanot, Alexandra, dos Santos, Wanderley D., Rezende, Camila A., McQueen-Mason, Simon J., Gomez, Leonardo D.
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container_end_page 124499
container_issue
container_start_page 124499
container_title Bioresource technology
container_volume 321
creator Mota, Thatiane R.
Oliveira, Dyoni M.
Simister, Rachael
Whitehead, Caragh
Lanot, Alexandra
dos Santos, Wanderley D.
Rezende, Camila A.
McQueen-Mason, Simon J.
Gomez, Leonardo D.
description •Simultaneous optimisation of pretreatment and saccharification using design tool.•High-resolution Design of Experiments maximizes sugar release in sugarcane bagasse.•Fractional Factorial Design was combined with a Central Composite Orthogonal design.•Design of Experiments monitored by compositional analysis of biomass. To maximize the sugar release from sugarcane bagasse, a high-resolution Fractional Factorial Design (FFD) was combined with a Central Composite Orthogonal (CCO) design to simultaneously evaluate a wide range of variables for alkaline pretreatment (NaOH: 0.1–1 mol/L, temperature: 100–220 °C, and time: 20–80 min) and enzymatic saccharification (enzyme loading: 2.5–17.5%, and reaction volume: 550–850 µL). A total of 46 experimental conditions were evaluated and the maximum sugar yield (423 mg/g) was obtained after 18 h enzymatic hydrolysis under optimized conditions (0.25 mol/L NaOH at 202 °C for 40 min, with 12.5% of enzyme loading). Biomass compositional analyses showed that the pretreatments strongly removed lignin (up to 70%), silica (up to 80%) and promoted cellulose enrichment (25–110%). This robust design of experiments resulted in maximizing enzymatic hydrolysis efficiency of sugarcane bagasse and further indicated that this combined approach is versatile for other lignocellulosic biomasses.
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To maximize the sugar release from sugarcane bagasse, a high-resolution Fractional Factorial Design (FFD) was combined with a Central Composite Orthogonal (CCO) design to simultaneously evaluate a wide range of variables for alkaline pretreatment (NaOH: 0.1–1 mol/L, temperature: 100–220 °C, and time: 20–80 min) and enzymatic saccharification (enzyme loading: 2.5–17.5%, and reaction volume: 550–850 µL). A total of 46 experimental conditions were evaluated and the maximum sugar yield (423 mg/g) was obtained after 18 h enzymatic hydrolysis under optimized conditions (0.25 mol/L NaOH at 202 °C for 40 min, with 12.5% of enzyme loading). Biomass compositional analyses showed that the pretreatments strongly removed lignin (up to 70%), silica (up to 80%) and promoted cellulose enrichment (25–110%). 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To maximize the sugar release from sugarcane bagasse, a high-resolution Fractional Factorial Design (FFD) was combined with a Central Composite Orthogonal (CCO) design to simultaneously evaluate a wide range of variables for alkaline pretreatment (NaOH: 0.1–1 mol/L, temperature: 100–220 °C, and time: 20–80 min) and enzymatic saccharification (enzyme loading: 2.5–17.5%, and reaction volume: 550–850 µL). A total of 46 experimental conditions were evaluated and the maximum sugar yield (423 mg/g) was obtained after 18 h enzymatic hydrolysis under optimized conditions (0.25 mol/L NaOH at 202 °C for 40 min, with 12.5% of enzyme loading). Biomass compositional analyses showed that the pretreatments strongly removed lignin (up to 70%), silica (up to 80%) and promoted cellulose enrichment (25–110%). This robust design of experiments resulted in maximizing enzymatic hydrolysis efficiency of sugarcane bagasse and further indicated that this combined approach is versatile for other lignocellulosic biomasses.</description><subject>alkali treatment</subject><subject>Alkaline pretreatment</subject><subject>biomass</subject><subject>Biomass pretreatment</subject><subject>Cellulose</subject><subject>Central composite orthogonal design</subject><subject>enzymatic hydrolysis</subject><subject>enzymes</subject><subject>Fractional factorial design</subject><subject>Hydrolysis</subject><subject>Lignin</subject><subject>Lignocellulose</subject><subject>saccharification</subject><subject>Saccharum</subject><subject>silica</subject><subject>sugarcane bagasse</subject><subject>sugars</subject><subject>temperature</subject><issn>0960-8524</issn><issn>1873-2976</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkctuFDEQRS0EIkPgF6JesumhbPfLO1B4SpHYwNoq2-WJh35heyLg6_HQCdusSiqdcpXvYeyKw54D794c9yYsMZO93QsQpSmaRqknbMeHXtZC9d1TtgPVQT20orlgL1I6AoDkvXjOLqSUHOTQ79j0nlI4zNXiK_q1UgwTzTlVLoY7Kt01hyn8wRyWfwiOP3AMM1VrpBwJ85mucHZVQmtvMQYf7Eb7JVbpdMBosfAGD5gSvWTPPI6JXt3XS_b944dv15_rm6-fvly_u6lt-UWuSTlE20roneGtb6UCP0DDufD9YAk5mU46VE0Lpmus80Zi2_TSGbBg0MhL9np7d43LzxOlrKeQLI1juWU5JS1KJkIJGLrH0aYvsQFXvKDdhtq4pBTJ67XkhfG35qDPVvRRP1jRZyt6s1IGr-53nMxE7v_Yg4YCvN0AKqHcBYo62UCzJRci2azdEh7b8ReYuaPu</recordid><startdate>202102</startdate><enddate>202102</enddate><creator>Mota, Thatiane R.</creator><creator>Oliveira, Dyoni M.</creator><creator>Simister, Rachael</creator><creator>Whitehead, Caragh</creator><creator>Lanot, Alexandra</creator><creator>dos Santos, Wanderley D.</creator><creator>Rezende, Camila A.</creator><creator>McQueen-Mason, Simon J.</creator><creator>Gomez, Leonardo D.</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope></search><sort><creationdate>202102</creationdate><title>Design of experiments driven optimization of alkaline pretreatment and saccharification for sugarcane bagasse</title><author>Mota, Thatiane R. ; 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subjects alkali treatment
Alkaline pretreatment
biomass
Biomass pretreatment
Cellulose
Central composite orthogonal design
enzymatic hydrolysis
enzymes
Fractional factorial design
Hydrolysis
Lignin
Lignocellulose
saccharification
Saccharum
silica
sugarcane bagasse
sugars
temperature
title Design of experiments driven optimization of alkaline pretreatment and saccharification for sugarcane bagasse
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