Design of experiments driven optimization of alkaline pretreatment and saccharification for sugarcane bagasse
•Simultaneous optimisation of pretreatment and saccharification using design tool.•High-resolution Design of Experiments maximizes sugar release in sugarcane bagasse.•Fractional Factorial Design was combined with a Central Composite Orthogonal design.•Design of Experiments monitored by compositional...
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Veröffentlicht in: | Bioresource technology 2021-02, Vol.321, p.124499-124499, Article 124499 |
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creator | Mota, Thatiane R. Oliveira, Dyoni M. Simister, Rachael Whitehead, Caragh Lanot, Alexandra dos Santos, Wanderley D. Rezende, Camila A. McQueen-Mason, Simon J. Gomez, Leonardo D. |
description | •Simultaneous optimisation of pretreatment and saccharification using design tool.•High-resolution Design of Experiments maximizes sugar release in sugarcane bagasse.•Fractional Factorial Design was combined with a Central Composite Orthogonal design.•Design of Experiments monitored by compositional analysis of biomass.
To maximize the sugar release from sugarcane bagasse, a high-resolution Fractional Factorial Design (FFD) was combined with a Central Composite Orthogonal (CCO) design to simultaneously evaluate a wide range of variables for alkaline pretreatment (NaOH: 0.1–1 mol/L, temperature: 100–220 °C, and time: 20–80 min) and enzymatic saccharification (enzyme loading: 2.5–17.5%, and reaction volume: 550–850 µL). A total of 46 experimental conditions were evaluated and the maximum sugar yield (423 mg/g) was obtained after 18 h enzymatic hydrolysis under optimized conditions (0.25 mol/L NaOH at 202 °C for 40 min, with 12.5% of enzyme loading). Biomass compositional analyses showed that the pretreatments strongly removed lignin (up to 70%), silica (up to 80%) and promoted cellulose enrichment (25–110%). This robust design of experiments resulted in maximizing enzymatic hydrolysis efficiency of sugarcane bagasse and further indicated that this combined approach is versatile for other lignocellulosic biomasses. |
doi_str_mv | 10.1016/j.biortech.2020.124499 |
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To maximize the sugar release from sugarcane bagasse, a high-resolution Fractional Factorial Design (FFD) was combined with a Central Composite Orthogonal (CCO) design to simultaneously evaluate a wide range of variables for alkaline pretreatment (NaOH: 0.1–1 mol/L, temperature: 100–220 °C, and time: 20–80 min) and enzymatic saccharification (enzyme loading: 2.5–17.5%, and reaction volume: 550–850 µL). A total of 46 experimental conditions were evaluated and the maximum sugar yield (423 mg/g) was obtained after 18 h enzymatic hydrolysis under optimized conditions (0.25 mol/L NaOH at 202 °C for 40 min, with 12.5% of enzyme loading). Biomass compositional analyses showed that the pretreatments strongly removed lignin (up to 70%), silica (up to 80%) and promoted cellulose enrichment (25–110%). This robust design of experiments resulted in maximizing enzymatic hydrolysis efficiency of sugarcane bagasse and further indicated that this combined approach is versatile for other lignocellulosic biomasses.</description><identifier>ISSN: 0960-8524</identifier><identifier>EISSN: 1873-2976</identifier><identifier>DOI: 10.1016/j.biortech.2020.124499</identifier><identifier>PMID: 33310387</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>alkali treatment ; Alkaline pretreatment ; biomass ; Biomass pretreatment ; Cellulose ; Central composite orthogonal design ; enzymatic hydrolysis ; enzymes ; Fractional factorial design ; Hydrolysis ; Lignin ; Lignocellulose ; saccharification ; Saccharum ; silica ; sugarcane bagasse ; sugars ; temperature</subject><ispartof>Bioresource technology, 2021-02, Vol.321, p.124499-124499, Article 124499</ispartof><rights>2020 Elsevier Ltd</rights><rights>Copyright © 2020 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c449t-e9daac5307db15f5390f804112f78cea1eb63da9450b64cdfb3a5473db0c0bab3</citedby><cites>FETCH-LOGICAL-c449t-e9daac5307db15f5390f804112f78cea1eb63da9450b64cdfb3a5473db0c0bab3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.biortech.2020.124499$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,778,782,3539,27911,27912,45982</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33310387$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mota, Thatiane R.</creatorcontrib><creatorcontrib>Oliveira, Dyoni M.</creatorcontrib><creatorcontrib>Simister, Rachael</creatorcontrib><creatorcontrib>Whitehead, Caragh</creatorcontrib><creatorcontrib>Lanot, Alexandra</creatorcontrib><creatorcontrib>dos Santos, Wanderley D.</creatorcontrib><creatorcontrib>Rezende, Camila A.</creatorcontrib><creatorcontrib>McQueen-Mason, Simon J.</creatorcontrib><creatorcontrib>Gomez, Leonardo D.</creatorcontrib><title>Design of experiments driven optimization of alkaline pretreatment and saccharification for sugarcane bagasse</title><title>Bioresource technology</title><addtitle>Bioresour Technol</addtitle><description>•Simultaneous optimisation of pretreatment and saccharification using design tool.•High-resolution Design of Experiments maximizes sugar release in sugarcane bagasse.•Fractional Factorial Design was combined with a Central Composite Orthogonal design.•Design of Experiments monitored by compositional analysis of biomass.
To maximize the sugar release from sugarcane bagasse, a high-resolution Fractional Factorial Design (FFD) was combined with a Central Composite Orthogonal (CCO) design to simultaneously evaluate a wide range of variables for alkaline pretreatment (NaOH: 0.1–1 mol/L, temperature: 100–220 °C, and time: 20–80 min) and enzymatic saccharification (enzyme loading: 2.5–17.5%, and reaction volume: 550–850 µL). A total of 46 experimental conditions were evaluated and the maximum sugar yield (423 mg/g) was obtained after 18 h enzymatic hydrolysis under optimized conditions (0.25 mol/L NaOH at 202 °C for 40 min, with 12.5% of enzyme loading). Biomass compositional analyses showed that the pretreatments strongly removed lignin (up to 70%), silica (up to 80%) and promoted cellulose enrichment (25–110%). This robust design of experiments resulted in maximizing enzymatic hydrolysis efficiency of sugarcane bagasse and further indicated that this combined approach is versatile for other lignocellulosic biomasses.</description><subject>alkali treatment</subject><subject>Alkaline pretreatment</subject><subject>biomass</subject><subject>Biomass pretreatment</subject><subject>Cellulose</subject><subject>Central composite orthogonal design</subject><subject>enzymatic hydrolysis</subject><subject>enzymes</subject><subject>Fractional factorial design</subject><subject>Hydrolysis</subject><subject>Lignin</subject><subject>Lignocellulose</subject><subject>saccharification</subject><subject>Saccharum</subject><subject>silica</subject><subject>sugarcane bagasse</subject><subject>sugars</subject><subject>temperature</subject><issn>0960-8524</issn><issn>1873-2976</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkctuFDEQRS0EIkPgF6JesumhbPfLO1B4SpHYwNoq2-WJh35heyLg6_HQCdusSiqdcpXvYeyKw54D794c9yYsMZO93QsQpSmaRqknbMeHXtZC9d1TtgPVQT20orlgL1I6AoDkvXjOLqSUHOTQ79j0nlI4zNXiK_q1UgwTzTlVLoY7Kt01hyn8wRyWfwiOP3AMM1VrpBwJ85mucHZVQmtvMQYf7Eb7JVbpdMBosfAGD5gSvWTPPI6JXt3XS_b944dv15_rm6-fvly_u6lt-UWuSTlE20roneGtb6UCP0DDufD9YAk5mU46VE0Lpmus80Zi2_TSGbBg0MhL9np7d43LzxOlrKeQLI1juWU5JS1KJkIJGLrH0aYvsQFXvKDdhtq4pBTJ67XkhfG35qDPVvRRP1jRZyt6s1IGr-53nMxE7v_Yg4YCvN0AKqHcBYo62UCzJRci2azdEh7b8ReYuaPu</recordid><startdate>202102</startdate><enddate>202102</enddate><creator>Mota, Thatiane R.</creator><creator>Oliveira, Dyoni M.</creator><creator>Simister, Rachael</creator><creator>Whitehead, Caragh</creator><creator>Lanot, Alexandra</creator><creator>dos Santos, Wanderley D.</creator><creator>Rezende, Camila A.</creator><creator>McQueen-Mason, Simon J.</creator><creator>Gomez, Leonardo D.</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope></search><sort><creationdate>202102</creationdate><title>Design of experiments driven optimization of alkaline pretreatment and saccharification for sugarcane bagasse</title><author>Mota, Thatiane R. ; Oliveira, Dyoni M. ; Simister, Rachael ; Whitehead, Caragh ; Lanot, Alexandra ; dos Santos, Wanderley D. ; Rezende, Camila A. ; McQueen-Mason, Simon J. ; Gomez, Leonardo D.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c449t-e9daac5307db15f5390f804112f78cea1eb63da9450b64cdfb3a5473db0c0bab3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>alkali treatment</topic><topic>Alkaline pretreatment</topic><topic>biomass</topic><topic>Biomass pretreatment</topic><topic>Cellulose</topic><topic>Central composite orthogonal design</topic><topic>enzymatic hydrolysis</topic><topic>enzymes</topic><topic>Fractional factorial design</topic><topic>Hydrolysis</topic><topic>Lignin</topic><topic>Lignocellulose</topic><topic>saccharification</topic><topic>Saccharum</topic><topic>silica</topic><topic>sugarcane bagasse</topic><topic>sugars</topic><topic>temperature</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mota, Thatiane R.</creatorcontrib><creatorcontrib>Oliveira, Dyoni M.</creatorcontrib><creatorcontrib>Simister, Rachael</creatorcontrib><creatorcontrib>Whitehead, Caragh</creatorcontrib><creatorcontrib>Lanot, Alexandra</creatorcontrib><creatorcontrib>dos Santos, Wanderley D.</creatorcontrib><creatorcontrib>Rezende, Camila A.</creatorcontrib><creatorcontrib>McQueen-Mason, Simon J.</creatorcontrib><creatorcontrib>Gomez, Leonardo D.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>Bioresource technology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mota, Thatiane R.</au><au>Oliveira, Dyoni M.</au><au>Simister, Rachael</au><au>Whitehead, Caragh</au><au>Lanot, Alexandra</au><au>dos Santos, Wanderley D.</au><au>Rezende, Camila A.</au><au>McQueen-Mason, Simon J.</au><au>Gomez, Leonardo D.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Design of experiments driven optimization of alkaline pretreatment and saccharification for sugarcane bagasse</atitle><jtitle>Bioresource technology</jtitle><addtitle>Bioresour Technol</addtitle><date>2021-02</date><risdate>2021</risdate><volume>321</volume><spage>124499</spage><epage>124499</epage><pages>124499-124499</pages><artnum>124499</artnum><issn>0960-8524</issn><eissn>1873-2976</eissn><abstract>•Simultaneous optimisation of pretreatment and saccharification using design tool.•High-resolution Design of Experiments maximizes sugar release in sugarcane bagasse.•Fractional Factorial Design was combined with a Central Composite Orthogonal design.•Design of Experiments monitored by compositional analysis of biomass.
To maximize the sugar release from sugarcane bagasse, a high-resolution Fractional Factorial Design (FFD) was combined with a Central Composite Orthogonal (CCO) design to simultaneously evaluate a wide range of variables for alkaline pretreatment (NaOH: 0.1–1 mol/L, temperature: 100–220 °C, and time: 20–80 min) and enzymatic saccharification (enzyme loading: 2.5–17.5%, and reaction volume: 550–850 µL). A total of 46 experimental conditions were evaluated and the maximum sugar yield (423 mg/g) was obtained after 18 h enzymatic hydrolysis under optimized conditions (0.25 mol/L NaOH at 202 °C for 40 min, with 12.5% of enzyme loading). Biomass compositional analyses showed that the pretreatments strongly removed lignin (up to 70%), silica (up to 80%) and promoted cellulose enrichment (25–110%). This robust design of experiments resulted in maximizing enzymatic hydrolysis efficiency of sugarcane bagasse and further indicated that this combined approach is versatile for other lignocellulosic biomasses.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>33310387</pmid><doi>10.1016/j.biortech.2020.124499</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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subjects | alkali treatment Alkaline pretreatment biomass Biomass pretreatment Cellulose Central composite orthogonal design enzymatic hydrolysis enzymes Fractional factorial design Hydrolysis Lignin Lignocellulose saccharification Saccharum silica sugarcane bagasse sugars temperature |
title | Design of experiments driven optimization of alkaline pretreatment and saccharification for sugarcane bagasse |
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