Light-emitting diodes and their potential in callus growth, plantlet development and saponin accumulation during somatic embryogenesis of Panax vietnamensis Ha et Grushv
In recent years, LED (light-emitting diode) has been the subject of research within the field of plant growth and development. However, there has been little discussion about using LED in vitro cultures of Panax vietnamensis, one of the important medicinal plants belonging to the Panax genus. This s...
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| Veröffentlicht in: | Biotechnology, biotechnological equipment biotechnological equipment, 2015-03, Vol.29 (2), p.299-308 |
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| creator | Nhut, Duong Tan Huy, Nguyen Phuc Tai, Ngo Thanh Nam, Nguyen Ba Luan, Vu Quoc Hien, Vu Thi Tung, Hoang Thanh Vinh, Bui The Luan, Tran Cong |
| description | In recent years, LED (light-emitting diode) has been the subject of research within the field of plant growth and development. However, there has been little discussion about using LED in vitro cultures of Panax vietnamensis, one of the important medicinal plants belonging to the Panax genus. This study examines the influence of various LED lamps on callus growth and plant formation of P. vietnamensis. Results show significant differences in growth and development, as various light conditions were suitable for different stages. Callus of 70 mg in fresh weight cultured under yellow LEDs resulted in growth of 1197 mg in fresh weight and 91.7 mg of dry weight, within a period of three months. The most effective plant formation was obtained when embryogenic calli were cultured under the combination of 60% red LED and 40% blue LED with an average of 11.21 plantlets per explant; the shoot clump fresh weight and dry weight were of 1147 and 127 mg, respectively, and the average plant height was 3.1 cm. It was also shown that this light condition was the most efficient for P. vietnamensis in vitro plant growth and development. This study provided additional evidence regarding the influence of different LEDs on ginsenoside production applying high-performance liquid chromatography (HPLC) analysis with photo-diode array (PDA) detection at ultraviolet (UV) wavelength 203 nm. The highest MR
2
content was recorded when plants maintained under 20% red LED combined with 80% blue LED. However, the highest Rg
1
and Rb
1
content was found under fluorescent light. The results presented might provide new strategies using LEDs for adequate micropropagation protocols of P. vietnamensis. |
| doi_str_mv | 10.1080/13102818.2014.1000210 |
| format | Article |
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2
content was recorded when plants maintained under 20% red LED combined with 80% blue LED. However, the highest Rg
1
and Rb
1
content was found under fluorescent light. The results presented might provide new strategies using LEDs for adequate micropropagation protocols of P. vietnamensis.</description><identifier>ISSN: 1310-2818</identifier><identifier>ISSN: 1314-3530</identifier><identifier>EISSN: 1314-3530</identifier><identifier>DOI: 10.1080/13102818.2014.1000210</identifier><identifier>PMID: 26019644</identifier><language>eng</language><publisher>England: Taylor & Francis</publisher><subject>Agriculture and Environmental Biotechnology ; biotechnology ; Callus ; Embryonic growth stage ; Fluorescence ; fluorescent lighting ; Ginsenosides ; Herbal medicine ; High performance liquid chromatography ; LEDs ; Light emitting diodes ; Liquid chromatography ; Medicinal plants ; Micropropagation ; Panax ; Panax vietnamensis ; Panax vietnamensis Ha et Grushv ; Plant growth ; plant height ; Plantlets ; Saponins ; Somatic embryogenesis ; spectrum ; wavelengths ; Weight</subject><ispartof>Biotechnology, biotechnological equipment, 2015-03, Vol.29 (2), p.299-308</ispartof><rights>2015 The Author(s). Published by Taylor & Francis. 2015</rights><rights>2015 The Author(s). Published by Taylor & Francis. This work is licensed under the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2015 The Author(s). Published by Taylor & Francis. 2015 The Author(s)</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c482t-5730a0daa46e3040c0a34ccd65e9e7367ae8a3f2842478581877cffe92d064b43</citedby><cites>FETCH-LOGICAL-c482t-5730a0daa46e3040c0a34ccd65e9e7367ae8a3f2842478581877cffe92d064b43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.tandfonline.com/doi/pdf/10.1080/13102818.2014.1000210$$EPDF$$P50$$Ginformaworld$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.tandfonline.com/doi/full/10.1080/13102818.2014.1000210$$EHTML$$P50$$Ginformaworld$$Hfree_for_read</linktohtml><link.rule.ids>230,314,776,780,860,881,27479,27901,27902,59116,59117</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26019644$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nhut, Duong Tan</creatorcontrib><creatorcontrib>Huy, Nguyen Phuc</creatorcontrib><creatorcontrib>Tai, Ngo Thanh</creatorcontrib><creatorcontrib>Nam, Nguyen Ba</creatorcontrib><creatorcontrib>Luan, Vu Quoc</creatorcontrib><creatorcontrib>Hien, Vu Thi</creatorcontrib><creatorcontrib>Tung, Hoang Thanh</creatorcontrib><creatorcontrib>Vinh, Bui The</creatorcontrib><creatorcontrib>Luan, Tran Cong</creatorcontrib><title>Light-emitting diodes and their potential in callus growth, plantlet development and saponin accumulation during somatic embryogenesis of Panax vietnamensis Ha et Grushv</title><title>Biotechnology, biotechnological equipment</title><addtitle>Biotechnol Biotechnol Equip</addtitle><description>In recent years, LED (light-emitting diode) has been the subject of research within the field of plant growth and development. However, there has been little discussion about using LED in vitro cultures of Panax vietnamensis, one of the important medicinal plants belonging to the Panax genus. This study examines the influence of various LED lamps on callus growth and plant formation of P. vietnamensis. Results show significant differences in growth and development, as various light conditions were suitable for different stages. Callus of 70 mg in fresh weight cultured under yellow LEDs resulted in growth of 1197 mg in fresh weight and 91.7 mg of dry weight, within a period of three months. The most effective plant formation was obtained when embryogenic calli were cultured under the combination of 60% red LED and 40% blue LED with an average of 11.21 plantlets per explant; the shoot clump fresh weight and dry weight were of 1147 and 127 mg, respectively, and the average plant height was 3.1 cm. It was also shown that this light condition was the most efficient for P. vietnamensis in vitro plant growth and development. This study provided additional evidence regarding the influence of different LEDs on ginsenoside production applying high-performance liquid chromatography (HPLC) analysis with photo-diode array (PDA) detection at ultraviolet (UV) wavelength 203 nm. The highest MR
2
content was recorded when plants maintained under 20% red LED combined with 80% blue LED. However, the highest Rg
1
and Rb
1
content was found under fluorescent light. The results presented might provide new strategies using LEDs for adequate micropropagation protocols of P. vietnamensis.</description><subject>Agriculture and Environmental Biotechnology</subject><subject>biotechnology</subject><subject>Callus</subject><subject>Embryonic growth stage</subject><subject>Fluorescence</subject><subject>fluorescent lighting</subject><subject>Ginsenosides</subject><subject>Herbal medicine</subject><subject>High performance liquid chromatography</subject><subject>LEDs</subject><subject>Light emitting diodes</subject><subject>Liquid chromatography</subject><subject>Medicinal plants</subject><subject>Micropropagation</subject><subject>Panax</subject><subject>Panax vietnamensis</subject><subject>Panax vietnamensis Ha et Grushv</subject><subject>Plant growth</subject><subject>plant height</subject><subject>Plantlets</subject><subject>Saponins</subject><subject>Somatic embryogenesis</subject><subject>spectrum</subject><subject>wavelengths</subject><subject>Weight</subject><issn>1310-2818</issn><issn>1314-3530</issn><issn>1314-3530</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>0YH</sourceid><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqFkk1v1DAQhiMEoqXwE0CWuHAgZfyRj70gUAUt0kpwgLM16zhZV44dbGfL_iT-JU53WwEHONkeP_OOZ_wWxXMK5xRaeEM5BdbS9pwBFTkEwCg8KE5zXJS84vDwdg_lAp0UT2K8BmgAaPO4OGE10FUtxGnxc22GbSr1aFIybiCd8Z2OBF1H0labQCaftEsGLTGOKLR2jmQI_iZtX5PJoktWJ9LpnbZ-GjN5mxpx8i7zqNQ8zhaT8Y50c1gqRD_msyJ63IS9H7TT0UTie_IFHf4gO6OTw6y0RK-QZPXLMMft7mnxqEcb9bPjelZ8-_jh68VVuf58-eni_bpUomWprBoOCB2iqDUHAQqQC6W6utIr3fC6Qd0i71krmGjaKg-naVTf6xXroBYbwc-Ktwfdad6MulO5p4BWTsGMGPbSo5F_3jizlYPfSSE4X8Ei8OooEPz3WcckRxOVtnlY2s9RsooJ1tYNsP-itGV1TZu64hl9-Rd67efg8iQkY2z5TF4vgtWBUsHHGHR__24KcvGNvPONXHwjj77JeS9-b_o-684oGXh3AIzrfRjxxgfbyYR760Mf0CkTJf93jV8DHtV8</recordid><startdate>20150304</startdate><enddate>20150304</enddate><creator>Nhut, Duong Tan</creator><creator>Huy, Nguyen Phuc</creator><creator>Tai, Ngo Thanh</creator><creator>Nam, Nguyen Ba</creator><creator>Luan, Vu Quoc</creator><creator>Hien, Vu Thi</creator><creator>Tung, Hoang Thanh</creator><creator>Vinh, Bui The</creator><creator>Luan, Tran Cong</creator><general>Taylor & Francis</general><general>Taylor & Francis Ltd</general><scope>0YH</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QO</scope><scope>7ST</scope><scope>7XB</scope><scope>8FD</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>M2O</scope><scope>MBDVC</scope><scope>P64</scope><scope>PHGZM</scope><scope>PHGZT</scope><scope>PKEHL</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>SOI</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope><scope>5PM</scope></search><sort><creationdate>20150304</creationdate><title>Light-emitting diodes and their potential in callus growth, plantlet development and saponin accumulation during somatic embryogenesis of Panax vietnamensis Ha et Grushv</title><author>Nhut, Duong Tan ; 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However, there has been little discussion about using LED in vitro cultures of Panax vietnamensis, one of the important medicinal plants belonging to the Panax genus. This study examines the influence of various LED lamps on callus growth and plant formation of P. vietnamensis. Results show significant differences in growth and development, as various light conditions were suitable for different stages. Callus of 70 mg in fresh weight cultured under yellow LEDs resulted in growth of 1197 mg in fresh weight and 91.7 mg of dry weight, within a period of three months. The most effective plant formation was obtained when embryogenic calli were cultured under the combination of 60% red LED and 40% blue LED with an average of 11.21 plantlets per explant; the shoot clump fresh weight and dry weight were of 1147 and 127 mg, respectively, and the average plant height was 3.1 cm. It was also shown that this light condition was the most efficient for P. vietnamensis in vitro plant growth and development. This study provided additional evidence regarding the influence of different LEDs on ginsenoside production applying high-performance liquid chromatography (HPLC) analysis with photo-diode array (PDA) detection at ultraviolet (UV) wavelength 203 nm. The highest MR
2
content was recorded when plants maintained under 20% red LED combined with 80% blue LED. However, the highest Rg
1
and Rb
1
content was found under fluorescent light. The results presented might provide new strategies using LEDs for adequate micropropagation protocols of P. vietnamensis.</abstract><cop>England</cop><pub>Taylor & Francis</pub><pmid>26019644</pmid><doi>10.1080/13102818.2014.1000210</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Biotechnology, biotechnological equipment, 2015-03, Vol.29 (2), p.299-308 |
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| subjects | Agriculture and Environmental Biotechnology biotechnology Callus Embryonic growth stage Fluorescence fluorescent lighting Ginsenosides Herbal medicine High performance liquid chromatography LEDs Light emitting diodes Liquid chromatography Medicinal plants Micropropagation Panax Panax vietnamensis Panax vietnamensis Ha et Grushv Plant growth plant height Plantlets Saponins Somatic embryogenesis spectrum wavelengths Weight |
| title | Light-emitting diodes and their potential in callus growth, plantlet development and saponin accumulation during somatic embryogenesis of Panax vietnamensis Ha et Grushv |
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