Distinct chromatin signature of histone H3 variant H3.3 in human cells
Actively transcribed regions of the genome have been found enriched for the histone H3 variant H3.3. This variant is incorporated into nucleosomes throughout the cell cycle whereas the canonical isoforms are predominately deposited in association with replication. In order to obtain a global picture...
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| Veröffentlicht in: | Nucleus (Austin, Tex.) Tex.), 2014-09, Vol.5 (5), p.449-461 |
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| creator | Snyers, Luc Zupkovitz, Gordin Almeder, Marlene Fliesser, Marianne Stoisser, Anja Weipoltshammer, Klara Schöfer, Christian |
| description | Actively transcribed regions of the genome have been found enriched for the histone H3 variant H3.3. This variant is incorporated into nucleosomes throughout the cell cycle whereas the canonical isoforms are predominately deposited in association with replication. In order to obtain a global picture of the deposition pattern at the single cell level we expressed H3.3 in both normal and malignant human cells and analyzed nuclei using conventional and structured illumination imaging (SIM). We found that the distribution pattern of H3.3 in interphase differs from that of the canonical histone H3 variants and this difference is conveyed to mitotic chromosomes which display a distinct H3.3 banding pattern. Histone H3.3 localization positively correlated with markers for transcriptionally active chromatin and, notably, H3.3 was almost completely absent from the inactive X chromosome. Collectively, our data show that histone variant H3.3 occupies distinct intranuclear chromatin domains and that these genomic loci are associated with gene expression. |
| doi_str_mv | 10.4161/nucl.36229 |
| format | Article |
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This variant is incorporated into nucleosomes throughout the cell cycle whereas the canonical isoforms are predominately deposited in association with replication. In order to obtain a global picture of the deposition pattern at the single cell level we expressed H3.3 in both normal and malignant human cells and analyzed nuclei using conventional and structured illumination imaging (SIM). We found that the distribution pattern of H3.3 in interphase differs from that of the canonical histone H3 variants and this difference is conveyed to mitotic chromosomes which display a distinct H3.3 banding pattern. Histone H3.3 localization positively correlated with markers for transcriptionally active chromatin and, notably, H3.3 was almost completely absent from the inactive X chromosome. Collectively, our data show that histone variant H3.3 occupies distinct intranuclear chromatin domains and that these genomic loci are associated with gene expression.</description><identifier>ISSN: 1949-1034</identifier><identifier>ISSN: 1949-1042</identifier><identifier>EISSN: 1949-1042</identifier><identifier>DOI: 10.4161/nucl.36229</identifier><identifier>PMID: 25482197</identifier><language>eng</language><publisher>United States: Taylor & Francis</publisher><subject>Cell Nucleus - genetics ; chromatin ; Chromatin - genetics ; Chromosomes, Human, X - genetics ; gene expression ; Gene Expression Regulation ; genome ; Genome, Human ; genomics ; histones ; Histones - genetics ; Humans ; interphase ; lighting ; mitosis ; nuclear architecture ; nucleosomes ; Nucleosomes - genetics ; Single-Cell Analysis ; superresolution imaging ; transcription ; transcription (genetics) ; Transcription, Genetic</subject><ispartof>Nucleus (Austin, Tex.), 2014-09, Vol.5 (5), p.449-461</ispartof><rights>Copyright © 2014 Landes Bioscience 2014</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c402t-a46a62ac106963ba28d4f63e505c6c1b1ebd0cf08b498e32b47641cb8008ced93</citedby><cites>FETCH-LOGICAL-c402t-a46a62ac106963ba28d4f63e505c6c1b1ebd0cf08b498e32b47641cb8008ced93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.tandfonline.com/doi/pdf/10.4161/nucl.36229$$EPDF$$P50$$Ginformaworld$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.tandfonline.com/doi/full/10.4161/nucl.36229$$EHTML$$P50$$Ginformaworld$$Hfree_for_read</linktohtml><link.rule.ids>314,776,780,27479,27901,27902,59116,59117</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25482197$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Snyers, Luc</creatorcontrib><creatorcontrib>Zupkovitz, Gordin</creatorcontrib><creatorcontrib>Almeder, Marlene</creatorcontrib><creatorcontrib>Fliesser, Marianne</creatorcontrib><creatorcontrib>Stoisser, Anja</creatorcontrib><creatorcontrib>Weipoltshammer, Klara</creatorcontrib><creatorcontrib>Schöfer, Christian</creatorcontrib><title>Distinct chromatin signature of histone H3 variant H3.3 in human cells</title><title>Nucleus (Austin, Tex.)</title><addtitle>Nucleus</addtitle><description>Actively transcribed regions of the genome have been found enriched for the histone H3 variant H3.3. This variant is incorporated into nucleosomes throughout the cell cycle whereas the canonical isoforms are predominately deposited in association with replication. In order to obtain a global picture of the deposition pattern at the single cell level we expressed H3.3 in both normal and malignant human cells and analyzed nuclei using conventional and structured illumination imaging (SIM). We found that the distribution pattern of H3.3 in interphase differs from that of the canonical histone H3 variants and this difference is conveyed to mitotic chromosomes which display a distinct H3.3 banding pattern. Histone H3.3 localization positively correlated with markers for transcriptionally active chromatin and, notably, H3.3 was almost completely absent from the inactive X chromosome. Collectively, our data show that histone variant H3.3 occupies distinct intranuclear chromatin domains and that these genomic loci are associated with gene expression.</description><subject>Cell Nucleus - genetics</subject><subject>chromatin</subject><subject>Chromatin - genetics</subject><subject>Chromosomes, Human, X - genetics</subject><subject>gene expression</subject><subject>Gene Expression Regulation</subject><subject>genome</subject><subject>Genome, Human</subject><subject>genomics</subject><subject>histones</subject><subject>Histones - genetics</subject><subject>Humans</subject><subject>interphase</subject><subject>lighting</subject><subject>mitosis</subject><subject>nuclear architecture</subject><subject>nucleosomes</subject><subject>Nucleosomes - genetics</subject><subject>Single-Cell Analysis</subject><subject>superresolution imaging</subject><subject>transcription</subject><subject>transcription (genetics)</subject><subject>Transcription, Genetic</subject><issn>1949-1034</issn><issn>1949-1042</issn><issn>1949-1042</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>0YH</sourceid><sourceid>EIF</sourceid><recordid>eNqFkF1LwzAUhoMobszd-AMklyJ05uM0bS9lOicMvNHrkKapi7TJTFpl_97OzV0Jnpvzwnl4OTwIXVIyAyroret1M-OCseIEjWkBRUIJsNNj5jBC0xjfyTAAGUnpORqxFHJGi2yMFvc2dtbpDut18K0aMo72zamuDwb7Gq-Hu3cGLzn-VMEq1w1xxvHArftWOaxN08QLdFarJprpYU_Q6-LhZb5MVs-PT_O7VaKBsC5RIJRgSlMiCsFLxfIKasFNSlItNC2pKSuia5KXUOSGsxIyAVSXOSG5NlXBJ-h637sJ_qM3sZOtjbsPlDO-j5KlDBhwCvRflAoOGUsFhQG92aM6-BiDqeUm2FaFraRE7izLnWX5Y3mArw69fdma6oj-Oh2AdA9YV_vQqi8fmkp2atv4UAfltI2S_1H8DQj2iIo</recordid><startdate>20140901</startdate><enddate>20140901</enddate><creator>Snyers, Luc</creator><creator>Zupkovitz, Gordin</creator><creator>Almeder, Marlene</creator><creator>Fliesser, Marianne</creator><creator>Stoisser, Anja</creator><creator>Weipoltshammer, Klara</creator><creator>Schöfer, Christian</creator><general>Taylor & Francis</general><scope>0YH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope></search><sort><creationdate>20140901</creationdate><title>Distinct chromatin signature of histone H3 variant H3.3 in human cells</title><author>Snyers, Luc ; Zupkovitz, Gordin ; Almeder, Marlene ; Fliesser, Marianne ; Stoisser, Anja ; Weipoltshammer, Klara ; Schöfer, Christian</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c402t-a46a62ac106963ba28d4f63e505c6c1b1ebd0cf08b498e32b47641cb8008ced93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Cell Nucleus - genetics</topic><topic>chromatin</topic><topic>Chromatin - genetics</topic><topic>Chromosomes, Human, X - genetics</topic><topic>gene expression</topic><topic>Gene Expression Regulation</topic><topic>genome</topic><topic>Genome, Human</topic><topic>genomics</topic><topic>histones</topic><topic>Histones - genetics</topic><topic>Humans</topic><topic>interphase</topic><topic>lighting</topic><topic>mitosis</topic><topic>nuclear architecture</topic><topic>nucleosomes</topic><topic>Nucleosomes - genetics</topic><topic>Single-Cell Analysis</topic><topic>superresolution imaging</topic><topic>transcription</topic><topic>transcription (genetics)</topic><topic>Transcription, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Snyers, Luc</creatorcontrib><creatorcontrib>Zupkovitz, Gordin</creatorcontrib><creatorcontrib>Almeder, Marlene</creatorcontrib><creatorcontrib>Fliesser, Marianne</creatorcontrib><creatorcontrib>Stoisser, Anja</creatorcontrib><creatorcontrib>Weipoltshammer, Klara</creatorcontrib><creatorcontrib>Schöfer, Christian</creatorcontrib><collection>Taylor & Francis Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>Nucleus (Austin, Tex.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Snyers, Luc</au><au>Zupkovitz, Gordin</au><au>Almeder, Marlene</au><au>Fliesser, Marianne</au><au>Stoisser, Anja</au><au>Weipoltshammer, Klara</au><au>Schöfer, Christian</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Distinct chromatin signature of histone H3 variant H3.3 in human cells</atitle><jtitle>Nucleus (Austin, Tex.)</jtitle><addtitle>Nucleus</addtitle><date>2014-09-01</date><risdate>2014</risdate><volume>5</volume><issue>5</issue><spage>449</spage><epage>461</epage><pages>449-461</pages><issn>1949-1034</issn><issn>1949-1042</issn><eissn>1949-1042</eissn><abstract>Actively transcribed regions of the genome have been found enriched for the histone H3 variant H3.3. This variant is incorporated into nucleosomes throughout the cell cycle whereas the canonical isoforms are predominately deposited in association with replication. In order to obtain a global picture of the deposition pattern at the single cell level we expressed H3.3 in both normal and malignant human cells and analyzed nuclei using conventional and structured illumination imaging (SIM). We found that the distribution pattern of H3.3 in interphase differs from that of the canonical histone H3 variants and this difference is conveyed to mitotic chromosomes which display a distinct H3.3 banding pattern. Histone H3.3 localization positively correlated with markers for transcriptionally active chromatin and, notably, H3.3 was almost completely absent from the inactive X chromosome. Collectively, our data show that histone variant H3.3 occupies distinct intranuclear chromatin domains and that these genomic loci are associated with gene expression.</abstract><cop>United States</cop><pub>Taylor & Francis</pub><pmid>25482197</pmid><doi>10.4161/nucl.36229</doi><tpages>13</tpages><oa>free_for_read</oa></addata></record> |
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| source | Taylor & Francis Open Access; MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central |
| subjects | Cell Nucleus - genetics chromatin Chromatin - genetics Chromosomes, Human, X - genetics gene expression Gene Expression Regulation genome Genome, Human genomics histones Histones - genetics Humans interphase lighting mitosis nuclear architecture nucleosomes Nucleosomes - genetics Single-Cell Analysis superresolution imaging transcription transcription (genetics) Transcription, Genetic |
| title | Distinct chromatin signature of histone H3 variant H3.3 in human cells |
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