Long noncoding RNA CTD-2245E15.3 promotes anabolic enzymes ACC1 and PC to support non-small cell lung cancer growth

Long noncoding RNAs (lncRNA) have been shown to play critical regulatory roles in the onset and progression of human cancers. However, the functions of a large proportion of lncRNAs are still unexplored. Here we describe a novel lncRNA, CTD-2245E15.3, that promotes lung tumorigenesis by regulating t...

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Veröffentlicht in:Cancer research (Chicago, Ill.) Ill.), 2021-07, Vol.81 (13), p.3509-3524
Hauptverfasser: Wang, Chen, Meng, Xiangfeng, Zhou, Yu, Yu, Jing, Li, Qing, Liao, Zhicong, Gu, Yuanyuan, Han, Jiayi, Linghu, Shuo, Jiao, Zichen, Wang, Tao, Zhang, Chen-Yu, Chen, Xi
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container_issue 13
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container_title Cancer research (Chicago, Ill.)
container_volume 81
creator Wang, Chen
Meng, Xiangfeng
Zhou, Yu
Yu, Jing
Li, Qing
Liao, Zhicong
Gu, Yuanyuan
Han, Jiayi
Linghu, Shuo
Jiao, Zichen
Wang, Tao
Zhang, Chen-Yu
Chen, Xi
description Long noncoding RNAs (lncRNA) have been shown to play critical regulatory roles in the onset and progression of human cancers. However, the functions of a large proportion of lncRNAs are still unexplored. Here we describe a novel lncRNA, CTD-2245E15.3, that promotes lung tumorigenesis by regulating the anabolic enzymes acetyl-CoA carboxylase 1 (ACC1) and pyruvate carboxylase (PC). Differentially expressed lncRNAs between non-small cell lung cancer (NSCLC) and paired adjacent nontumor tissues were identified by a microarray and validated using quantitative real-time polymerase chain reaction. CTD-2245E15.3 was significantly upregulated in NSCLC and was mainly located in the cytoplasm. Knockdown of CTD-2245E15.3 by specific antisense oligonucleotides suppressed cell growth in vitro and in vivo, largely due to cell cycle arrest and induction of apoptosis. Overexpression of CTD-2245E15.3 in an orthotopic model of lung cancer led to a significant increase in total tumor burden. CTD-2245E15.3 exerted its oncogenic function by binding ACC1 and PC, which are key anabolic factors for biomolecule synthesis in rapidly proliferating tumor cells. Knockdown of CTD-2245E15.3 increased phosphorylation of ACC1 at an inhibitory site for enzymatic activity and promoted PC degradation via ubiquitination. Supplements of palmitate or oxaloacetate, products of ACC1 and PC, alleviated the suppression of cell growth caused by loss of CTD-2245E15.3. These findings reveal the important role of CTD-2245E15.3 as an oncogenic lncRNA in the anabolic process for tumor growth.
doi_str_mv 10.1158/0008-5472.CAN-19-3806
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title Long noncoding RNA CTD-2245E15.3 promotes anabolic enzymes ACC1 and PC to support non-small cell lung cancer growth
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