Targeting DDX3X Triggers Antitumor Immunity via a dsRNA-Mediated Tumor-Intrinsic Type I Interferon Response
Induction of nucleic acid sensing-mediated type I interferon (IFN) has emerged as a novel approach to activate the immune system against cancer. Here we show that the depletion of DEAD-box RNA helicase 3X (DDX3X) triggers a tumor-intrinsic type I IFN response in breast cancer cells. Depletion or inh...
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| Veröffentlicht in: | Cancer research (Chicago, Ill.) Ill.), 2021-07, Vol.81 (13), p.3607-3620 |
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| container_title | Cancer research (Chicago, Ill.) |
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| creator | Choi, Hyeongjwa Kwon, Juntae Cho, Min Soon Sun, Yifan Zheng, Xiaofeng Wang, Jing Bouker, Kerrie B Casey, John L Atkins, Michael B Toretsky, Jeffrey Han, Cecil |
| description | Induction of nucleic acid sensing-mediated type I interferon (IFN) has emerged as a novel approach to activate the immune system against cancer. Here we show that the depletion of DEAD-box RNA helicase 3X (DDX3X) triggers a tumor-intrinsic type I IFN response in breast cancer cells. Depletion or inhibition of DDX3X activity led to aberrant cytoplasmic accumulation of cellular endogenous double-stranded RNAs (dsRNA), which triggered type I IFN production through the melanoma differentiation-associated gene 5 (MDA5)-mediated dsRNA-sensing pathway. Furthermore, DDX3X interacted with dsRNA-editing ADAR1 and dual depletion of DDX3X and ADAR1 synergistically activated the cytosolic dsRNA pathway in breast cancer cells. Loss of DDX3X in mouse mammary tumors enhanced antitumor activity by increasing the tumor-intrinsic type I IFN response, antigen presentation, and tumor infiltration of cytotoxic T and dendritic cells. These findings may lead to the development of a novel therapeutic approach for breast cancer by targeting DDX3X in combination with immune-checkpoint blockade. SIGNIFICANCE: This study elucidates the novel role of DDX3X in regulating endogenous cellular dsRNA homeostasis and type I IFN signaling in breast cancer. GRAPHICAL ABSTRACT: http://cancerres.aacrjournals.org/content/canres/81/13/3607/F1.large.jpg. |
| doi_str_mv | 10.1158/0008-5472.CAN-20-3790 |
| format | Article |
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Here we show that the depletion of DEAD-box RNA helicase 3X (DDX3X) triggers a tumor-intrinsic type I IFN response in breast cancer cells. Depletion or inhibition of DDX3X activity led to aberrant cytoplasmic accumulation of cellular endogenous double-stranded RNAs (dsRNA), which triggered type I IFN production through the melanoma differentiation-associated gene 5 (MDA5)-mediated dsRNA-sensing pathway. Furthermore, DDX3X interacted with dsRNA-editing ADAR1 and dual depletion of DDX3X and ADAR1 synergistically activated the cytosolic dsRNA pathway in breast cancer cells. Loss of DDX3X in mouse mammary tumors enhanced antitumor activity by increasing the tumor-intrinsic type I IFN response, antigen presentation, and tumor infiltration of cytotoxic T and dendritic cells. These findings may lead to the development of a novel therapeutic approach for breast cancer by targeting DDX3X in combination with immune-checkpoint blockade. SIGNIFICANCE: This study elucidates the novel role of DDX3X in regulating endogenous cellular dsRNA homeostasis and type I IFN signaling in breast cancer. GRAPHICAL ABSTRACT: http://cancerres.aacrjournals.org/content/canres/81/13/3607/F1.large.jpg.</description><identifier>ISSN: 0008-5472</identifier><identifier>ISSN: 1538-7445</identifier><identifier>EISSN: 1538-7445</identifier><identifier>DOI: 10.1158/0008-5472.CAN-20-3790</identifier><identifier>PMID: 33941613</identifier><language>eng</language><publisher>United States</publisher><subject>Animals ; Apoptosis ; Biomarkers, Tumor - genetics ; Biomarkers, Tumor - metabolism ; Breast Neoplasms - immunology ; Breast Neoplasms - metabolism ; Breast Neoplasms - pathology ; Breast Neoplasms - prevention & control ; Cell Proliferation ; DEAD-box RNA Helicases - antagonists & inhibitors ; DEAD-box RNA Helicases - genetics ; DEAD-box RNA Helicases - metabolism ; Female ; Gene Expression Regulation, Neoplastic ; Humans ; Immunity, Innate - immunology ; Interferon Type I - immunology ; Interferon Type I - metabolism ; Interferon-Induced Helicase, IFIH1 - genetics ; Interferon-Induced Helicase, IFIH1 - metabolism ; Mice ; Mice, Inbred BALB C ; Mice, Nude ; Prognosis ; RNA, Double-Stranded - genetics ; Survival Rate ; Tumor Cells, Cultured ; Xenograft Model Antitumor Assays</subject><ispartof>Cancer research (Chicago, Ill.), 2021-07, Vol.81 (13), p.3607-3620</ispartof><rights>2021 American Association for Cancer Research.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c460t-36940fa9ffaa0974cc559dbad8848c5ecaeb40d4e5c74d8f5d825eb3b422e5723</citedby><cites>FETCH-LOGICAL-c460t-36940fa9ffaa0974cc559dbad8848c5ecaeb40d4e5c74d8f5d825eb3b422e5723</cites><orcidid>0000-0001-6205-0837 ; 0000-0001-6276-168X ; 0000-0002-4896-9692</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,3343,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33941613$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Choi, Hyeongjwa</creatorcontrib><creatorcontrib>Kwon, Juntae</creatorcontrib><creatorcontrib>Cho, Min Soon</creatorcontrib><creatorcontrib>Sun, Yifan</creatorcontrib><creatorcontrib>Zheng, Xiaofeng</creatorcontrib><creatorcontrib>Wang, Jing</creatorcontrib><creatorcontrib>Bouker, Kerrie B</creatorcontrib><creatorcontrib>Casey, John L</creatorcontrib><creatorcontrib>Atkins, Michael B</creatorcontrib><creatorcontrib>Toretsky, Jeffrey</creatorcontrib><creatorcontrib>Han, Cecil</creatorcontrib><title>Targeting DDX3X Triggers Antitumor Immunity via a dsRNA-Mediated Tumor-Intrinsic Type I Interferon Response</title><title>Cancer research (Chicago, Ill.)</title><addtitle>Cancer Res</addtitle><description>Induction of nucleic acid sensing-mediated type I interferon (IFN) has emerged as a novel approach to activate the immune system against cancer. Here we show that the depletion of DEAD-box RNA helicase 3X (DDX3X) triggers a tumor-intrinsic type I IFN response in breast cancer cells. Depletion or inhibition of DDX3X activity led to aberrant cytoplasmic accumulation of cellular endogenous double-stranded RNAs (dsRNA), which triggered type I IFN production through the melanoma differentiation-associated gene 5 (MDA5)-mediated dsRNA-sensing pathway. Furthermore, DDX3X interacted with dsRNA-editing ADAR1 and dual depletion of DDX3X and ADAR1 synergistically activated the cytosolic dsRNA pathway in breast cancer cells. Loss of DDX3X in mouse mammary tumors enhanced antitumor activity by increasing the tumor-intrinsic type I IFN response, antigen presentation, and tumor infiltration of cytotoxic T and dendritic cells. These findings may lead to the development of a novel therapeutic approach for breast cancer by targeting DDX3X in combination with immune-checkpoint blockade. SIGNIFICANCE: This study elucidates the novel role of DDX3X in regulating endogenous cellular dsRNA homeostasis and type I IFN signaling in breast cancer. GRAPHICAL ABSTRACT: http://cancerres.aacrjournals.org/content/canres/81/13/3607/F1.large.jpg.</description><subject>Animals</subject><subject>Apoptosis</subject><subject>Biomarkers, Tumor - genetics</subject><subject>Biomarkers, Tumor - metabolism</subject><subject>Breast Neoplasms - immunology</subject><subject>Breast Neoplasms - metabolism</subject><subject>Breast Neoplasms - pathology</subject><subject>Breast Neoplasms - prevention & control</subject><subject>Cell Proliferation</subject><subject>DEAD-box RNA Helicases - antagonists & inhibitors</subject><subject>DEAD-box RNA Helicases - genetics</subject><subject>DEAD-box RNA Helicases - metabolism</subject><subject>Female</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>Humans</subject><subject>Immunity, Innate - immunology</subject><subject>Interferon Type I - immunology</subject><subject>Interferon Type I - metabolism</subject><subject>Interferon-Induced Helicase, IFIH1 - genetics</subject><subject>Interferon-Induced Helicase, IFIH1 - metabolism</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Mice, Nude</subject><subject>Prognosis</subject><subject>RNA, Double-Stranded - genetics</subject><subject>Survival Rate</subject><subject>Tumor Cells, Cultured</subject><subject>Xenograft Model Antitumor Assays</subject><issn>0008-5472</issn><issn>1538-7445</issn><issn>1538-7445</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kF1LwzAUhoMobk5_gpJLbzLzubaXY_Nj4AeMCt6FNDkd0bWdSSvs39vi3NXhPTzvOfAgdM3olDGV3lFKU6JkwqeL-SvhlIgkoydozJRISSKlOkXjIzNCFzF-9lExqs7RSIhMshkTY_SVm7CB1tcbvFx-iA-cB7_ZQIh4Xre-7aom4FVVdbVv9_jHG2ywi-vXOXkB500LDucDQ1Z1G3wdvcX5fgd4hfsFhBJCU-M1xF1TR7hEZ6XZRrg6zAl6f7jPF0_k-e1xtZg_EytntCVilklamqwsjaFZIq1VKnOFcWkqU6vAGigkdRKUTaRLS-VSrqAQheQcVMLFBN3-3d2F5ruD2OrKRwvbramh6aLminOWMSqzHlV_qA1NjAFKvQu-MmGvGdWDZz041IND3XvWnOrBc9-7ObzoigrcsfUvVvwC3oJ5tw</recordid><startdate>20210701</startdate><enddate>20210701</enddate><creator>Choi, Hyeongjwa</creator><creator>Kwon, Juntae</creator><creator>Cho, Min Soon</creator><creator>Sun, Yifan</creator><creator>Zheng, Xiaofeng</creator><creator>Wang, Jing</creator><creator>Bouker, Kerrie B</creator><creator>Casey, John L</creator><creator>Atkins, Michael B</creator><creator>Toretsky, Jeffrey</creator><creator>Han, Cecil</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-6205-0837</orcidid><orcidid>https://orcid.org/0000-0001-6276-168X</orcidid><orcidid>https://orcid.org/0000-0002-4896-9692</orcidid></search><sort><creationdate>20210701</creationdate><title>Targeting DDX3X Triggers Antitumor Immunity via a dsRNA-Mediated Tumor-Intrinsic Type I Interferon Response</title><author>Choi, Hyeongjwa ; Kwon, Juntae ; Cho, Min Soon ; Sun, Yifan ; Zheng, Xiaofeng ; Wang, Jing ; Bouker, Kerrie B ; Casey, John L ; Atkins, Michael B ; Toretsky, Jeffrey ; Han, Cecil</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c460t-36940fa9ffaa0974cc559dbad8848c5ecaeb40d4e5c74d8f5d825eb3b422e5723</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Animals</topic><topic>Apoptosis</topic><topic>Biomarkers, Tumor - genetics</topic><topic>Biomarkers, Tumor - metabolism</topic><topic>Breast Neoplasms - immunology</topic><topic>Breast Neoplasms - metabolism</topic><topic>Breast Neoplasms - pathology</topic><topic>Breast Neoplasms - prevention & control</topic><topic>Cell Proliferation</topic><topic>DEAD-box RNA Helicases - antagonists & inhibitors</topic><topic>DEAD-box RNA Helicases - genetics</topic><topic>DEAD-box RNA Helicases - metabolism</topic><topic>Female</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>Humans</topic><topic>Immunity, Innate - immunology</topic><topic>Interferon Type I - immunology</topic><topic>Interferon Type I - metabolism</topic><topic>Interferon-Induced Helicase, IFIH1 - genetics</topic><topic>Interferon-Induced Helicase, IFIH1 - metabolism</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Mice, Nude</topic><topic>Prognosis</topic><topic>RNA, Double-Stranded - genetics</topic><topic>Survival Rate</topic><topic>Tumor Cells, Cultured</topic><topic>Xenograft Model Antitumor Assays</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Choi, Hyeongjwa</creatorcontrib><creatorcontrib>Kwon, Juntae</creatorcontrib><creatorcontrib>Cho, Min Soon</creatorcontrib><creatorcontrib>Sun, Yifan</creatorcontrib><creatorcontrib>Zheng, Xiaofeng</creatorcontrib><creatorcontrib>Wang, Jing</creatorcontrib><creatorcontrib>Bouker, Kerrie B</creatorcontrib><creatorcontrib>Casey, John L</creatorcontrib><creatorcontrib>Atkins, Michael B</creatorcontrib><creatorcontrib>Toretsky, Jeffrey</creatorcontrib><creatorcontrib>Han, Cecil</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Cancer research (Chicago, Ill.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Choi, Hyeongjwa</au><au>Kwon, Juntae</au><au>Cho, Min Soon</au><au>Sun, Yifan</au><au>Zheng, Xiaofeng</au><au>Wang, Jing</au><au>Bouker, Kerrie B</au><au>Casey, John L</au><au>Atkins, Michael B</au><au>Toretsky, Jeffrey</au><au>Han, Cecil</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Targeting DDX3X Triggers Antitumor Immunity via a dsRNA-Mediated Tumor-Intrinsic Type I Interferon Response</atitle><jtitle>Cancer research (Chicago, Ill.)</jtitle><addtitle>Cancer Res</addtitle><date>2021-07-01</date><risdate>2021</risdate><volume>81</volume><issue>13</issue><spage>3607</spage><epage>3620</epage><pages>3607-3620</pages><issn>0008-5472</issn><issn>1538-7445</issn><eissn>1538-7445</eissn><abstract>Induction of nucleic acid sensing-mediated type I interferon (IFN) has emerged as a novel approach to activate the immune system against cancer. Here we show that the depletion of DEAD-box RNA helicase 3X (DDX3X) triggers a tumor-intrinsic type I IFN response in breast cancer cells. Depletion or inhibition of DDX3X activity led to aberrant cytoplasmic accumulation of cellular endogenous double-stranded RNAs (dsRNA), which triggered type I IFN production through the melanoma differentiation-associated gene 5 (MDA5)-mediated dsRNA-sensing pathway. Furthermore, DDX3X interacted with dsRNA-editing ADAR1 and dual depletion of DDX3X and ADAR1 synergistically activated the cytosolic dsRNA pathway in breast cancer cells. Loss of DDX3X in mouse mammary tumors enhanced antitumor activity by increasing the tumor-intrinsic type I IFN response, antigen presentation, and tumor infiltration of cytotoxic T and dendritic cells. These findings may lead to the development of a novel therapeutic approach for breast cancer by targeting DDX3X in combination with immune-checkpoint blockade. SIGNIFICANCE: This study elucidates the novel role of DDX3X in regulating endogenous cellular dsRNA homeostasis and type I IFN signaling in breast cancer. GRAPHICAL ABSTRACT: http://cancerres.aacrjournals.org/content/canres/81/13/3607/F1.large.jpg.</abstract><cop>United States</cop><pmid>33941613</pmid><doi>10.1158/0008-5472.CAN-20-3790</doi><tpages>14</tpages><orcidid>https://orcid.org/0000-0001-6205-0837</orcidid><orcidid>https://orcid.org/0000-0001-6276-168X</orcidid><orcidid>https://orcid.org/0000-0002-4896-9692</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Apoptosis Biomarkers, Tumor - genetics Biomarkers, Tumor - metabolism Breast Neoplasms - immunology Breast Neoplasms - metabolism Breast Neoplasms - pathology Breast Neoplasms - prevention & control Cell Proliferation DEAD-box RNA Helicases - antagonists & inhibitors DEAD-box RNA Helicases - genetics DEAD-box RNA Helicases - metabolism Female Gene Expression Regulation, Neoplastic Humans Immunity, Innate - immunology Interferon Type I - immunology Interferon Type I - metabolism Interferon-Induced Helicase, IFIH1 - genetics Interferon-Induced Helicase, IFIH1 - metabolism Mice Mice, Inbred BALB C Mice, Nude Prognosis RNA, Double-Stranded - genetics Survival Rate Tumor Cells, Cultured Xenograft Model Antitumor Assays |
| title | Targeting DDX3X Triggers Antitumor Immunity via a dsRNA-Mediated Tumor-Intrinsic Type I Interferon Response |
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