Gene knockout revealed the role of gene feoA in cell growth and division of Lactobacillus delbrueckii subsp. bulgaricus
Gene feoA plays an important role in cell growth because of its function of transport Fe 2+ which is a necessary element for cells. In this study, the recombinant plasmid pUC19-feoA-Tet was successfully constructed using the inserted gene inactivation method. Using the homologous recombination techn...
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| Veröffentlicht in: | Archives of microbiology 2021-08, Vol.203 (6), p.3541-3549 |
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| creator | Han, Xue Tu, Yuanqiang Wu, Huiying Zhang, Lijuan Zhao, Sainan |
| description | Gene
feoA
plays an important role in cell growth because of its function of transport Fe
2+
which is a necessary element for cells. In this study, the recombinant plasmid pUC19-feoA-Tet was successfully constructed using the inserted gene inactivation method. Using the homologous recombination technique, the
tet
gene was used as a resistance screening marker to knock out the
feoA
gene of
Lactobacillus delbrueckii
subsp.
bulgaricus
34.5 (strain 34.5). Comparative analysis of growth curves revealed the growth changes in the absence of
feoA
gene in strain 34.5. The results showed that the growth of the bacteria was prolonged by 2 h and could be restored in the stationary phase. To further study whether
feoA
is related to the cell division of strain 34.5, the qPCR experiment was carried out. The results showed that, compared with the wild-type strain, the expression of genes related to cell division in the mutant strain was up-regulated in the pre-log phase, down-regulated in the late-log phase, and returned to the original level in the stationary phase. These findings provide ideas for
Lactobacillus delbrueckii
subsp.
bulgaricus
to control division and cell cycle. |
| doi_str_mv | 10.1007/s00203-021-02345-z |
| format | Article |
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feoA
plays an important role in cell growth because of its function of transport Fe
2+
which is a necessary element for cells. In this study, the recombinant plasmid pUC19-feoA-Tet was successfully constructed using the inserted gene inactivation method. Using the homologous recombination technique, the
tet
gene was used as a resistance screening marker to knock out the
feoA
gene of
Lactobacillus delbrueckii
subsp.
bulgaricus
34.5 (strain 34.5). Comparative analysis of growth curves revealed the growth changes in the absence of
feoA
gene in strain 34.5. The results showed that the growth of the bacteria was prolonged by 2 h and could be restored in the stationary phase. To further study whether
feoA
is related to the cell division of strain 34.5, the qPCR experiment was carried out. The results showed that, compared with the wild-type strain, the expression of genes related to cell division in the mutant strain was up-regulated in the pre-log phase, down-regulated in the late-log phase, and returned to the original level in the stationary phase. These findings provide ideas for
Lactobacillus delbrueckii
subsp.
bulgaricus
to control division and cell cycle.</description><identifier>ISSN: 0302-8933</identifier><identifier>EISSN: 1432-072X</identifier><identifier>DOI: 10.1007/s00203-021-02345-z</identifier><identifier>PMID: 33942158</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Biochemistry ; Biomedical and Life Sciences ; Biotechnology ; Cell Biology ; Cell cycle ; Cell division ; Cell growth ; Comparative analysis ; Ecology ; Gene expression ; Growth curves ; Homologous recombination ; Homology ; Inactivation ; Iron ; Lactobacilli ; Lactobacillus delbrueckii ; Life Sciences ; Microbial Ecology ; Microbiology ; Original Paper ; Stationary phase ; Tet gene</subject><ispartof>Archives of microbiology, 2021-08, Vol.203 (6), p.3541-3549</ispartof><rights>The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2021</rights><rights>The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2021.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c375t-6b1e0bb69da0919547310ce8b49cc6bf0d0741db5581780033eda22f57c807743</citedby><cites>FETCH-LOGICAL-c375t-6b1e0bb69da0919547310ce8b49cc6bf0d0741db5581780033eda22f57c807743</cites><orcidid>0000-0002-5276-8631</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00203-021-02345-z$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00203-021-02345-z$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,777,781,27905,27906,41469,42538,51300</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33942158$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Han, Xue</creatorcontrib><creatorcontrib>Tu, Yuanqiang</creatorcontrib><creatorcontrib>Wu, Huiying</creatorcontrib><creatorcontrib>Zhang, Lijuan</creatorcontrib><creatorcontrib>Zhao, Sainan</creatorcontrib><title>Gene knockout revealed the role of gene feoA in cell growth and division of Lactobacillus delbrueckii subsp. bulgaricus</title><title>Archives of microbiology</title><addtitle>Arch Microbiol</addtitle><addtitle>Arch Microbiol</addtitle><description>Gene
feoA
plays an important role in cell growth because of its function of transport Fe
2+
which is a necessary element for cells. In this study, the recombinant plasmid pUC19-feoA-Tet was successfully constructed using the inserted gene inactivation method. Using the homologous recombination technique, the
tet
gene was used as a resistance screening marker to knock out the
feoA
gene of
Lactobacillus delbrueckii
subsp.
bulgaricus
34.5 (strain 34.5). Comparative analysis of growth curves revealed the growth changes in the absence of
feoA
gene in strain 34.5. The results showed that the growth of the bacteria was prolonged by 2 h and could be restored in the stationary phase. To further study whether
feoA
is related to the cell division of strain 34.5, the qPCR experiment was carried out. The results showed that, compared with the wild-type strain, the expression of genes related to cell division in the mutant strain was up-regulated in the pre-log phase, down-regulated in the late-log phase, and returned to the original level in the stationary phase. These findings provide ideas for
Lactobacillus delbrueckii
subsp.
bulgaricus
to control division and cell cycle.</description><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>Cell Biology</subject><subject>Cell cycle</subject><subject>Cell division</subject><subject>Cell growth</subject><subject>Comparative analysis</subject><subject>Ecology</subject><subject>Gene expression</subject><subject>Growth curves</subject><subject>Homologous recombination</subject><subject>Homology</subject><subject>Inactivation</subject><subject>Iron</subject><subject>Lactobacilli</subject><subject>Lactobacillus delbrueckii</subject><subject>Life Sciences</subject><subject>Microbial Ecology</subject><subject>Microbiology</subject><subject>Original Paper</subject><subject>Stationary phase</subject><subject>Tet gene</subject><issn>0302-8933</issn><issn>1432-072X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kb1uFDEURi1ERDaBF6BAlmhoJlzb47WnjKIQIq2Uhkh0ln_ubJydHS_2OBF5embZABJFiisX93yfbR1C3jM4YwDqcwHgIBrgbB7RyubpFVmwVvAGFP_-mixAAG90J8QxOSnlHoBxrfUbcixE13Im9YI8XuGIdDMmv0l1ohkf0A4Y6HSHNKcBaerpeo_0mM5pHKnHYaDrnB6nO2rHQEN8iCWmcQ-urJ-Ssz4OQy004OByRb-JkZbqyu6MujqsbY6-lrfkqLdDwXfP5ym5_XL57eJrs7q5ur44XzVeKDk1S8cQnFt2wULHOtkqwcCjdm3n_dL1EEC1LDgpNVMaQAgMlvNeKq9BqVackk-H3l1OPyqWyWxj2f_BjphqMVxyzjpQUs_ox__Q-1TzOL9upqRgXHYCZoofKJ9TKRl7s8txa_NPw8DstZiDFjNrMb-1mKc59OG5urothr-RPx5mQByAMq_GNeZ_d79Q-wvL3Zh5</recordid><startdate>20210801</startdate><enddate>20210801</enddate><creator>Han, Xue</creator><creator>Tu, Yuanqiang</creator><creator>Wu, Huiying</creator><creator>Zhang, Lijuan</creator><creator>Zhao, Sainan</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-5276-8631</orcidid></search><sort><creationdate>20210801</creationdate><title>Gene knockout revealed the role of gene feoA in cell growth and division of Lactobacillus delbrueckii subsp. bulgaricus</title><author>Han, Xue ; Tu, Yuanqiang ; Wu, Huiying ; Zhang, Lijuan ; Zhao, Sainan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c375t-6b1e0bb69da0919547310ce8b49cc6bf0d0741db5581780033eda22f57c807743</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Biotechnology</topic><topic>Cell Biology</topic><topic>Cell cycle</topic><topic>Cell division</topic><topic>Cell growth</topic><topic>Comparative analysis</topic><topic>Ecology</topic><topic>Gene expression</topic><topic>Growth curves</topic><topic>Homologous recombination</topic><topic>Homology</topic><topic>Inactivation</topic><topic>Iron</topic><topic>Lactobacilli</topic><topic>Lactobacillus delbrueckii</topic><topic>Life Sciences</topic><topic>Microbial Ecology</topic><topic>Microbiology</topic><topic>Original Paper</topic><topic>Stationary phase</topic><topic>Tet gene</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Han, Xue</creatorcontrib><creatorcontrib>Tu, Yuanqiang</creatorcontrib><creatorcontrib>Wu, Huiying</creatorcontrib><creatorcontrib>Zhang, Lijuan</creatorcontrib><creatorcontrib>Zhao, Sainan</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection (Proquest)</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>ProQuest Biological Science Journals</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Archives of microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Han, Xue</au><au>Tu, Yuanqiang</au><au>Wu, Huiying</au><au>Zhang, Lijuan</au><au>Zhao, Sainan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Gene knockout revealed the role of gene feoA in cell growth and division of Lactobacillus delbrueckii subsp. bulgaricus</atitle><jtitle>Archives of microbiology</jtitle><stitle>Arch Microbiol</stitle><addtitle>Arch Microbiol</addtitle><date>2021-08-01</date><risdate>2021</risdate><volume>203</volume><issue>6</issue><spage>3541</spage><epage>3549</epage><pages>3541-3549</pages><issn>0302-8933</issn><eissn>1432-072X</eissn><abstract>Gene
feoA
plays an important role in cell growth because of its function of transport Fe
2+
which is a necessary element for cells. In this study, the recombinant plasmid pUC19-feoA-Tet was successfully constructed using the inserted gene inactivation method. Using the homologous recombination technique, the
tet
gene was used as a resistance screening marker to knock out the
feoA
gene of
Lactobacillus delbrueckii
subsp.
bulgaricus
34.5 (strain 34.5). Comparative analysis of growth curves revealed the growth changes in the absence of
feoA
gene in strain 34.5. The results showed that the growth of the bacteria was prolonged by 2 h and could be restored in the stationary phase. To further study whether
feoA
is related to the cell division of strain 34.5, the qPCR experiment was carried out. The results showed that, compared with the wild-type strain, the expression of genes related to cell division in the mutant strain was up-regulated in the pre-log phase, down-regulated in the late-log phase, and returned to the original level in the stationary phase. These findings provide ideas for
Lactobacillus delbrueckii
subsp.
bulgaricus
to control division and cell cycle.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>33942158</pmid><doi>10.1007/s00203-021-02345-z</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0002-5276-8631</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0302-8933 |
| ispartof | Archives of microbiology, 2021-08, Vol.203 (6), p.3541-3549 |
| issn | 0302-8933 1432-072X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2522190758 |
| source | SpringerLink Journals - AutoHoldings |
| subjects | Biochemistry Biomedical and Life Sciences Biotechnology Cell Biology Cell cycle Cell division Cell growth Comparative analysis Ecology Gene expression Growth curves Homologous recombination Homology Inactivation Iron Lactobacilli Lactobacillus delbrueckii Life Sciences Microbial Ecology Microbiology Original Paper Stationary phase Tet gene |
| title | Gene knockout revealed the role of gene feoA in cell growth and division of Lactobacillus delbrueckii subsp. bulgaricus |
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