Can serum matrix metalloproteinase‐9 and SMAD‐2 levels predict lamina propria invasion in bladder urothelial carcinoma?
Introduction Matrix metalloproteinase‐9 (MMP‐9) has a role in the destruction of lamina propria (LP) of the bladder wall and SMAD‐2 promotes cell‐to‐cell adhesion. This study aimed to investigate the association between LP invasion and serum protein and mRNA expression levels of MMP‐9 and SMAD‐2 in...
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| Veröffentlicht in: | International journal of clinical practice (Esher) 2021-08, Vol.75 (8), p.e14277-n/a |
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| creator | Yitgin, Yasin Erdem, Selcuk Serilmez, Murat Duranyıldız, Derya Verep, Samed Gasimov, Kamranbay Tefik, Tzevat Sanli, Oner Ozcan, Faruk |
| description | Introduction
Matrix metalloproteinase‐9 (MMP‐9) has a role in the destruction of lamina propria (LP) of the bladder wall and SMAD‐2 promotes cell‐to‐cell adhesion. This study aimed to investigate the association between LP invasion and serum protein and mRNA expression levels of MMP‐9 and SMAD‐2 in bladder cancer (BC) patients.
Methods
Serum samples were taken from 57 patients with suspicious BC before TUR‐BT (Group 1) and 20 patients with benign diseases as control (Group 2). The mRNA expression and serum protein levels of MMP‐9 and SMAD‐2 were analyzed using Real‐Time PCR and ELISA methods, respectively. The comparison of protein and mRNA expression levels of MMP‐9 and SMAD‐2 were done statistically between Group 1 and 2, as well as for different T stages of BC.
Results
The protein levels of MMP‐9 (2448 vs 637.5 pg/mL, P = .0001) and SMAD‐2 (6.85 vs 1.61 P = .0001) were significantly higher in Group 1 compared to Group 2. The mRNA expression levels of MMP‐9 (P = .89) and SMAD‐2 (P = .99) did not significantly differ between the groups. The protein levels of MMP‐9 in T1 patients were significantly higher from both of pTa patients (P = .018) and pT2 (P = .02). The protein levels of SMAD‐2 were not statistically different between T stages. Similarly, the mRNA expression levels of MMP‐9 and SMAD‐2 were not different between T stages.
Conclusions
The protein levels of MMP‐9 and SMAD‐2 were increased in BC patients while mRNA expressions were not different. Furthermore, the increased protein level of MMP‐9 in T1 patients was more pronounced which may be related to LP invasion of the tumor. |
| doi_str_mv | 10.1111/ijcp.14277 |
| format | Article |
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Matrix metalloproteinase‐9 (MMP‐9) has a role in the destruction of lamina propria (LP) of the bladder wall and SMAD‐2 promotes cell‐to‐cell adhesion. This study aimed to investigate the association between LP invasion and serum protein and mRNA expression levels of MMP‐9 and SMAD‐2 in bladder cancer (BC) patients.
Methods
Serum samples were taken from 57 patients with suspicious BC before TUR‐BT (Group 1) and 20 patients with benign diseases as control (Group 2). The mRNA expression and serum protein levels of MMP‐9 and SMAD‐2 were analyzed using Real‐Time PCR and ELISA methods, respectively. The comparison of protein and mRNA expression levels of MMP‐9 and SMAD‐2 were done statistically between Group 1 and 2, as well as for different T stages of BC.
Results
The protein levels of MMP‐9 (2448 vs 637.5 pg/mL, P = .0001) and SMAD‐2 (6.85 vs 1.61 P = .0001) were significantly higher in Group 1 compared to Group 2. The mRNA expression levels of MMP‐9 (P = .89) and SMAD‐2 (P = .99) did not significantly differ between the groups. The protein levels of MMP‐9 in T1 patients were significantly higher from both of pTa patients (P = .018) and pT2 (P = .02). The protein levels of SMAD‐2 were not statistically different between T stages. Similarly, the mRNA expression levels of MMP‐9 and SMAD‐2 were not different between T stages.
Conclusions
The protein levels of MMP‐9 and SMAD‐2 were increased in BC patients while mRNA expressions were not different. Furthermore, the increased protein level of MMP‐9 in T1 patients was more pronounced which may be related to LP invasion of the tumor.</description><identifier>ISSN: 1368-5031</identifier><identifier>EISSN: 1742-1241</identifier><identifier>DOI: 10.1111/ijcp.14277</identifier><identifier>PMID: 33928727</identifier><language>eng</language><publisher>India: Hindawi Limited</publisher><subject>Bladder ; Bladder cancer ; Cancer ; Carcinoma, Transitional Cell ; Cell adhesion ; Gene expression ; Humans ; Kinases ; Lamina propria ; Matrix metalloproteinase ; Matrix Metalloproteinase 9 - genetics ; Metalloproteinase ; Mucous Membrane ; Proteins ; Smad protein ; Smad2 Protein ; Urinary Bladder Neoplasms ; Urothelial carcinoma</subject><ispartof>International journal of clinical practice (Esher), 2021-08, Vol.75 (8), p.e14277-n/a</ispartof><rights>2021 John Wiley & Sons Ltd</rights><rights>2021 John Wiley & Sons Ltd.</rights><rights>Copyright © 2021 John Wiley & Sons Ltd</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c3527-ae123ba01ab6c1fac2d074ad566fdf8e346b6113997d2d391af9720d404808603</cites><orcidid>0000-0001-8502-2505 ; 0000-0001-8866-5573 ; 0000-0003-1111-6941 ; 0000-0001-5801-6898 ; 0000-0001-9187-451X ; 0000-0002-1398-8332 ; 0000-0002-3514-1394 ; 0000-0002-1086-6380 ; 0000-0001-7544-6638</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fijcp.14277$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fijcp.14277$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,777,781,1412,27905,27906,45555,45556</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33928727$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yitgin, Yasin</creatorcontrib><creatorcontrib>Erdem, Selcuk</creatorcontrib><creatorcontrib>Serilmez, Murat</creatorcontrib><creatorcontrib>Duranyıldız, Derya</creatorcontrib><creatorcontrib>Verep, Samed</creatorcontrib><creatorcontrib>Gasimov, Kamranbay</creatorcontrib><creatorcontrib>Tefik, Tzevat</creatorcontrib><creatorcontrib>Sanli, Oner</creatorcontrib><creatorcontrib>Ozcan, Faruk</creatorcontrib><title>Can serum matrix metalloproteinase‐9 and SMAD‐2 levels predict lamina propria invasion in bladder urothelial carcinoma?</title><title>International journal of clinical practice (Esher)</title><addtitle>Int J Clin Pract</addtitle><description>Introduction
Matrix metalloproteinase‐9 (MMP‐9) has a role in the destruction of lamina propria (LP) of the bladder wall and SMAD‐2 promotes cell‐to‐cell adhesion. This study aimed to investigate the association between LP invasion and serum protein and mRNA expression levels of MMP‐9 and SMAD‐2 in bladder cancer (BC) patients.
Methods
Serum samples were taken from 57 patients with suspicious BC before TUR‐BT (Group 1) and 20 patients with benign diseases as control (Group 2). The mRNA expression and serum protein levels of MMP‐9 and SMAD‐2 were analyzed using Real‐Time PCR and ELISA methods, respectively. The comparison of protein and mRNA expression levels of MMP‐9 and SMAD‐2 were done statistically between Group 1 and 2, as well as for different T stages of BC.
Results
The protein levels of MMP‐9 (2448 vs 637.5 pg/mL, P = .0001) and SMAD‐2 (6.85 vs 1.61 P = .0001) were significantly higher in Group 1 compared to Group 2. The mRNA expression levels of MMP‐9 (P = .89) and SMAD‐2 (P = .99) did not significantly differ between the groups. The protein levels of MMP‐9 in T1 patients were significantly higher from both of pTa patients (P = .018) and pT2 (P = .02). The protein levels of SMAD‐2 were not statistically different between T stages. Similarly, the mRNA expression levels of MMP‐9 and SMAD‐2 were not different between T stages.
Conclusions
The protein levels of MMP‐9 and SMAD‐2 were increased in BC patients while mRNA expressions were not different. Furthermore, the increased protein level of MMP‐9 in T1 patients was more pronounced which may be related to LP invasion of the tumor.</description><subject>Bladder</subject><subject>Bladder cancer</subject><subject>Cancer</subject><subject>Carcinoma, Transitional Cell</subject><subject>Cell adhesion</subject><subject>Gene expression</subject><subject>Humans</subject><subject>Kinases</subject><subject>Lamina propria</subject><subject>Matrix metalloproteinase</subject><subject>Matrix Metalloproteinase 9 - genetics</subject><subject>Metalloproteinase</subject><subject>Mucous Membrane</subject><subject>Proteins</subject><subject>Smad protein</subject><subject>Smad2 Protein</subject><subject>Urinary Bladder Neoplasms</subject><subject>Urothelial carcinoma</subject><issn>1368-5031</issn><issn>1742-1241</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kctu1TAQhq0K1Bvd9AGQJTYIKcW3xMkKVQcKRa2KBF1HE3ui-shJDnZSqNjwCDwjT8KUU1iwYDaekb75NNbP2LEUJ5LqZVi7zYk0ytodti-tUYVURj6iXld1UQot99hBzmshVFnWYpftad2o2iq7z76tYOQZ0zLwAeYUvvIBZ4hx2qRpxjBCxp_ffzQcRs8_Xp6-pkHxiLcYM98k9MHNPMJAII20FICH8RZymEZqeBfBe0x8IdsNxgCRO0gujNMAr56wxz3EjEcP7yG7PnvzafWuuLh6e746vSicLpUtAKXSHQgJXeVkD055YQ34sqp639eoTdVVUuqmsV553UjoG6uEN8LUoq6EPmTPt1668POCeW6HkB3GCCNOS25VqURdC-IJffYPup6WNNJ1RJXamNIYTdSLLeXSlHPCvqWPD5DuWina-0ja-0ja35EQ_PRBuXQD-r_onwwIkFvgS4h49x9Ve_5-9WEr_QV8K5hx</recordid><startdate>202108</startdate><enddate>202108</enddate><creator>Yitgin, Yasin</creator><creator>Erdem, Selcuk</creator><creator>Serilmez, Murat</creator><creator>Duranyıldız, Derya</creator><creator>Verep, Samed</creator><creator>Gasimov, Kamranbay</creator><creator>Tefik, Tzevat</creator><creator>Sanli, Oner</creator><creator>Ozcan, Faruk</creator><general>Hindawi Limited</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7T5</scope><scope>7TK</scope><scope>7TS</scope><scope>7U9</scope><scope>H94</scope><scope>K9.</scope><scope>NAPCQ</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-8502-2505</orcidid><orcidid>https://orcid.org/0000-0001-8866-5573</orcidid><orcidid>https://orcid.org/0000-0003-1111-6941</orcidid><orcidid>https://orcid.org/0000-0001-5801-6898</orcidid><orcidid>https://orcid.org/0000-0001-9187-451X</orcidid><orcidid>https://orcid.org/0000-0002-1398-8332</orcidid><orcidid>https://orcid.org/0000-0002-3514-1394</orcidid><orcidid>https://orcid.org/0000-0002-1086-6380</orcidid><orcidid>https://orcid.org/0000-0001-7544-6638</orcidid></search><sort><creationdate>202108</creationdate><title>Can serum matrix metalloproteinase‐9 and SMAD‐2 levels predict lamina propria invasion in bladder urothelial carcinoma?</title><author>Yitgin, Yasin ; Erdem, Selcuk ; Serilmez, Murat ; Duranyıldız, Derya ; Verep, Samed ; Gasimov, Kamranbay ; Tefik, Tzevat ; Sanli, Oner ; Ozcan, Faruk</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3527-ae123ba01ab6c1fac2d074ad566fdf8e346b6113997d2d391af9720d404808603</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Bladder</topic><topic>Bladder cancer</topic><topic>Cancer</topic><topic>Carcinoma, Transitional Cell</topic><topic>Cell adhesion</topic><topic>Gene expression</topic><topic>Humans</topic><topic>Kinases</topic><topic>Lamina propria</topic><topic>Matrix metalloproteinase</topic><topic>Matrix Metalloproteinase 9 - genetics</topic><topic>Metalloproteinase</topic><topic>Mucous Membrane</topic><topic>Proteins</topic><topic>Smad protein</topic><topic>Smad2 Protein</topic><topic>Urinary Bladder Neoplasms</topic><topic>Urothelial carcinoma</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yitgin, Yasin</creatorcontrib><creatorcontrib>Erdem, Selcuk</creatorcontrib><creatorcontrib>Serilmez, Murat</creatorcontrib><creatorcontrib>Duranyıldız, Derya</creatorcontrib><creatorcontrib>Verep, Samed</creatorcontrib><creatorcontrib>Gasimov, Kamranbay</creatorcontrib><creatorcontrib>Tefik, Tzevat</creatorcontrib><creatorcontrib>Sanli, Oner</creatorcontrib><creatorcontrib>Ozcan, Faruk</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Physical Education Index</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Premium</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of clinical practice (Esher)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yitgin, Yasin</au><au>Erdem, Selcuk</au><au>Serilmez, Murat</au><au>Duranyıldız, Derya</au><au>Verep, Samed</au><au>Gasimov, Kamranbay</au><au>Tefik, Tzevat</au><au>Sanli, Oner</au><au>Ozcan, Faruk</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Can serum matrix metalloproteinase‐9 and SMAD‐2 levels predict lamina propria invasion in bladder urothelial carcinoma?</atitle><jtitle>International journal of clinical practice (Esher)</jtitle><addtitle>Int J Clin Pract</addtitle><date>2021-08</date><risdate>2021</risdate><volume>75</volume><issue>8</issue><spage>e14277</spage><epage>n/a</epage><pages>e14277-n/a</pages><issn>1368-5031</issn><eissn>1742-1241</eissn><abstract>Introduction
Matrix metalloproteinase‐9 (MMP‐9) has a role in the destruction of lamina propria (LP) of the bladder wall and SMAD‐2 promotes cell‐to‐cell adhesion. This study aimed to investigate the association between LP invasion and serum protein and mRNA expression levels of MMP‐9 and SMAD‐2 in bladder cancer (BC) patients.
Methods
Serum samples were taken from 57 patients with suspicious BC before TUR‐BT (Group 1) and 20 patients with benign diseases as control (Group 2). The mRNA expression and serum protein levels of MMP‐9 and SMAD‐2 were analyzed using Real‐Time PCR and ELISA methods, respectively. The comparison of protein and mRNA expression levels of MMP‐9 and SMAD‐2 were done statistically between Group 1 and 2, as well as for different T stages of BC.
Results
The protein levels of MMP‐9 (2448 vs 637.5 pg/mL, P = .0001) and SMAD‐2 (6.85 vs 1.61 P = .0001) were significantly higher in Group 1 compared to Group 2. The mRNA expression levels of MMP‐9 (P = .89) and SMAD‐2 (P = .99) did not significantly differ between the groups. The protein levels of MMP‐9 in T1 patients were significantly higher from both of pTa patients (P = .018) and pT2 (P = .02). The protein levels of SMAD‐2 were not statistically different between T stages. Similarly, the mRNA expression levels of MMP‐9 and SMAD‐2 were not different between T stages.
Conclusions
The protein levels of MMP‐9 and SMAD‐2 were increased in BC patients while mRNA expressions were not different. Furthermore, the increased protein level of MMP‐9 in T1 patients was more pronounced which may be related to LP invasion of the tumor.</abstract><cop>India</cop><pub>Hindawi Limited</pub><pmid>33928727</pmid><doi>10.1111/ijcp.14277</doi><tpages>5</tpages><orcidid>https://orcid.org/0000-0001-8502-2505</orcidid><orcidid>https://orcid.org/0000-0001-8866-5573</orcidid><orcidid>https://orcid.org/0000-0003-1111-6941</orcidid><orcidid>https://orcid.org/0000-0001-5801-6898</orcidid><orcidid>https://orcid.org/0000-0001-9187-451X</orcidid><orcidid>https://orcid.org/0000-0002-1398-8332</orcidid><orcidid>https://orcid.org/0000-0002-3514-1394</orcidid><orcidid>https://orcid.org/0000-0002-1086-6380</orcidid><orcidid>https://orcid.org/0000-0001-7544-6638</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Bladder Bladder cancer Cancer Carcinoma, Transitional Cell Cell adhesion Gene expression Humans Kinases Lamina propria Matrix metalloproteinase Matrix Metalloproteinase 9 - genetics Metalloproteinase Mucous Membrane Proteins Smad protein Smad2 Protein Urinary Bladder Neoplasms Urothelial carcinoma |
| title | Can serum matrix metalloproteinase‐9 and SMAD‐2 levels predict lamina propria invasion in bladder urothelial carcinoma? |
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