Characterization and Monitoring of a Novel Light-heavy-light Chain Mispair in a Therapeutic Bispecific Antibody

Site-specific cysteine engineering, along with other genetic mutations, is broadly implemented in bispecific antibodies (bsAb). Thus far, homodimer, half hole antibody, one-light chain mispaired and light chain swapped variants have been reported as chain-pairing variants for the asymmetric IgG-like...

Ausführliche Beschreibung

Bibliographische Detailangaben

Veröffentlicht in:	Journal of pharmaceutical sciences 2021-08, Vol.110 (8), p.2904-2915
Hauptverfasser:	Cao, Mingyan, Parthemore, Conner, Jiao, Yang, Korman, Samuel, Aspelund, Matthew, Hunter, Alan, Kilby, Greg, Chen, Xiaoyu
Format:	Artikel
Sprache:	eng
Schlagworte:	Bispecific antibodies Correct pairing of cognate heavy chains Disulfide bond mapping Engineered cysteines Heterodimerization of heavy chains Knob-into-hole (KiH) light chains Mispair
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!