The effects of different potato dextrose agar media on secondary metabolite production in Fusarium
Potatoes contain several nutrients essential for fungal growth, making them an excellent component of media such as the popular Potato Dextrose Agar (PDA) medium. Commercially, PDA is available from multiple retailers offering virtually the same product. These media, however, could contain small dif...
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| Veröffentlicht in: | International journal of food microbiology 2021-06, Vol.347, p.109171-109171, Article 109171 |
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| container_title | International journal of food microbiology |
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| creator | Westphal, Klaus Ringsborg Heidelbach, Søren Zeuner, Emil Juel Riisgaard-Jensen, Marie Nielsen, Morten Eneberg Vestergaard, Sofie Zacho Bekker, Nicolai Sundgaard Skovmark, Jesper Olesen, Christian Kjær Thomsen, Katrine Hartmann Niebling, Sara Kramer Sørensen, Jens Laurids Sondergaard, Teis Esben |
| description | Potatoes contain several nutrients essential for fungal growth, making them an excellent component of media such as the popular Potato Dextrose Agar (PDA) medium. Commercially, PDA is available from multiple retailers offering virtually the same product. These media, however, could contain small differences in composition of nutrients affecting the expression of secondary metabolites. This study aims to investigate the use of four PDA media from different manufacturers (Fluka, Oxoid, Sigma, and VWR) and their effect on the metabolite profile of four species of Fusarium (F. fujikuroi, F. graminearum, F. pseudograminearum and F. avenaceum). Secondary metabolites were analysed using HPLC-HRMS, from which statistically significant differences in intensities were observed for 9 out of 10 metabolites.
•The choice of PDA manufacturer has an effect on the metabolite profile of several Fusarium species.•Custom made PDA is equally suited for metabolite experiments compared to commercially available PDA.•PDA induces secondary metabolite production in Fusaria. |
| doi_str_mv | 10.1016/j.ijfoodmicro.2021.109171 |
| format | Article |
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•The choice of PDA manufacturer has an effect on the metabolite profile of several Fusarium species.•Custom made PDA is equally suited for metabolite experiments compared to commercially available PDA.•PDA induces secondary metabolite production in Fusaria.</description><identifier>ISSN: 0168-1605</identifier><identifier>EISSN: 1879-3460</identifier><identifier>DOI: 10.1016/j.ijfoodmicro.2021.109171</identifier><identifier>PMID: 33872940</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Dextrose ; Fusarium ; High-performance liquid chromatography ; Liquid chromatography ; Metabolites ; Mycotoxins ; Nutrients ; Potato dextrose agar ; Potatoes ; Regulation ; Secondary metabolites ; Solanine ; Statistical analysis</subject><ispartof>International journal of food microbiology, 2021-06, Vol.347, p.109171-109171, Article 109171</ispartof><rights>2021</rights><rights>Copyright © 2021. Published by Elsevier B.V.</rights><rights>Copyright Elsevier BV Jun 2, 2021</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c405t-44e64b55d162cdbb55a8a510f950d6fd16c6c7bd526742dd3f1c8cdd7219491e3</citedby><cites>FETCH-LOGICAL-c405t-44e64b55d162cdbb55a8a510f950d6fd16c6c7bd526742dd3f1c8cdd7219491e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.ijfoodmicro.2021.109171$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33872940$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Westphal, Klaus Ringsborg</creatorcontrib><creatorcontrib>Heidelbach, Søren</creatorcontrib><creatorcontrib>Zeuner, Emil Juel</creatorcontrib><creatorcontrib>Riisgaard-Jensen, Marie</creatorcontrib><creatorcontrib>Nielsen, Morten Eneberg</creatorcontrib><creatorcontrib>Vestergaard, Sofie Zacho</creatorcontrib><creatorcontrib>Bekker, Nicolai Sundgaard</creatorcontrib><creatorcontrib>Skovmark, Jesper</creatorcontrib><creatorcontrib>Olesen, Christian Kjær</creatorcontrib><creatorcontrib>Thomsen, Katrine Hartmann</creatorcontrib><creatorcontrib>Niebling, Sara Kramer</creatorcontrib><creatorcontrib>Sørensen, Jens Laurids</creatorcontrib><creatorcontrib>Sondergaard, Teis Esben</creatorcontrib><title>The effects of different potato dextrose agar media on secondary metabolite production in Fusarium</title><title>International journal of food microbiology</title><addtitle>Int J Food Microbiol</addtitle><description>Potatoes contain several nutrients essential for fungal growth, making them an excellent component of media such as the popular Potato Dextrose Agar (PDA) medium. Commercially, PDA is available from multiple retailers offering virtually the same product. These media, however, could contain small differences in composition of nutrients affecting the expression of secondary metabolites. This study aims to investigate the use of four PDA media from different manufacturers (Fluka, Oxoid, Sigma, and VWR) and their effect on the metabolite profile of four species of Fusarium (F. fujikuroi, F. graminearum, F. pseudograminearum and F. avenaceum). Secondary metabolites were analysed using HPLC-HRMS, from which statistically significant differences in intensities were observed for 9 out of 10 metabolites.
•The choice of PDA manufacturer has an effect on the metabolite profile of several Fusarium species.•Custom made PDA is equally suited for metabolite experiments compared to commercially available PDA.•PDA induces secondary metabolite production in Fusaria.</description><subject>Dextrose</subject><subject>Fusarium</subject><subject>High-performance liquid chromatography</subject><subject>Liquid chromatography</subject><subject>Metabolites</subject><subject>Mycotoxins</subject><subject>Nutrients</subject><subject>Potato dextrose agar</subject><subject>Potatoes</subject><subject>Regulation</subject><subject>Secondary metabolites</subject><subject>Solanine</subject><subject>Statistical analysis</subject><issn>0168-1605</issn><issn>1879-3460</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNqNkUFv3CAQhVGVqtmk_QsVVS69eAsYsH2sVkkTKVIu6RlhGFqstdkCjtp_34k2jaKecmL0-IYZ3iPkE2dbzrj-Mm3jFFLyc3Q5bQUTHPWBd_wN2fC-G5pWanZCNsj2DddMnZKzUibGmGpb9o6ctm3fiUGyDRnvfwKFEMDVQlOgPmKdYan0kKqtiXr4XXMqQO0Pm-kMPlqaFlrApcXb_Aelase0jxXoISe_uhrxPi70ai02x3V-T94Guy_w4ek8J9-vLu93183t3beb3dfbxkmmaiMlaDkq5bkWzo9Y2d4qzsKgmNcBZaddN3oldCeF923grnfed4IPcuDQnpPPx3dxjV8rlGrmWBzs93aBtBYjFFe6l0IrRC_-Q6e05gW3Q6odlOoEk0gNRwpdLiVDMIccZ_yz4cw8BmEm8yII8xiEOQaBvR-fJqwjmvbc-c95BHZHANCShwjZFBdhcWhwxjCMT_EVY_4CtYqgLQ</recordid><startdate>20210602</startdate><enddate>20210602</enddate><creator>Westphal, Klaus Ringsborg</creator><creator>Heidelbach, Søren</creator><creator>Zeuner, Emil Juel</creator><creator>Riisgaard-Jensen, Marie</creator><creator>Nielsen, Morten Eneberg</creator><creator>Vestergaard, Sofie Zacho</creator><creator>Bekker, Nicolai Sundgaard</creator><creator>Skovmark, Jesper</creator><creator>Olesen, Christian Kjær</creator><creator>Thomsen, Katrine Hartmann</creator><creator>Niebling, Sara Kramer</creator><creator>Sørensen, Jens Laurids</creator><creator>Sondergaard, Teis Esben</creator><general>Elsevier B.V</general><general>Elsevier BV</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7QR</scope><scope>7T7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20210602</creationdate><title>The effects of different potato dextrose agar media on secondary metabolite production in Fusarium</title><author>Westphal, Klaus Ringsborg ; Heidelbach, Søren ; Zeuner, Emil Juel ; Riisgaard-Jensen, Marie ; Nielsen, Morten Eneberg ; Vestergaard, Sofie Zacho ; Bekker, Nicolai Sundgaard ; Skovmark, Jesper ; Olesen, Christian Kjær ; Thomsen, Katrine Hartmann ; Niebling, Sara Kramer ; Sørensen, Jens Laurids ; Sondergaard, Teis Esben</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c405t-44e64b55d162cdbb55a8a510f950d6fd16c6c7bd526742dd3f1c8cdd7219491e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Dextrose</topic><topic>Fusarium</topic><topic>High-performance liquid chromatography</topic><topic>Liquid chromatography</topic><topic>Metabolites</topic><topic>Mycotoxins</topic><topic>Nutrients</topic><topic>Potato dextrose agar</topic><topic>Potatoes</topic><topic>Regulation</topic><topic>Secondary metabolites</topic><topic>Solanine</topic><topic>Statistical analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Westphal, Klaus Ringsborg</creatorcontrib><creatorcontrib>Heidelbach, Søren</creatorcontrib><creatorcontrib>Zeuner, Emil Juel</creatorcontrib><creatorcontrib>Riisgaard-Jensen, Marie</creatorcontrib><creatorcontrib>Nielsen, Morten Eneberg</creatorcontrib><creatorcontrib>Vestergaard, Sofie Zacho</creatorcontrib><creatorcontrib>Bekker, Nicolai Sundgaard</creatorcontrib><creatorcontrib>Skovmark, Jesper</creatorcontrib><creatorcontrib>Olesen, Christian Kjær</creatorcontrib><creatorcontrib>Thomsen, Katrine Hartmann</creatorcontrib><creatorcontrib>Niebling, Sara Kramer</creatorcontrib><creatorcontrib>Sørensen, Jens Laurids</creatorcontrib><creatorcontrib>Sondergaard, Teis Esben</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of food microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Westphal, Klaus Ringsborg</au><au>Heidelbach, Søren</au><au>Zeuner, Emil Juel</au><au>Riisgaard-Jensen, Marie</au><au>Nielsen, Morten Eneberg</au><au>Vestergaard, Sofie Zacho</au><au>Bekker, Nicolai Sundgaard</au><au>Skovmark, Jesper</au><au>Olesen, Christian Kjær</au><au>Thomsen, Katrine Hartmann</au><au>Niebling, Sara Kramer</au><au>Sørensen, Jens Laurids</au><au>Sondergaard, Teis Esben</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The effects of different potato dextrose agar media on secondary metabolite production in Fusarium</atitle><jtitle>International journal of food microbiology</jtitle><addtitle>Int J Food Microbiol</addtitle><date>2021-06-02</date><risdate>2021</risdate><volume>347</volume><spage>109171</spage><epage>109171</epage><pages>109171-109171</pages><artnum>109171</artnum><issn>0168-1605</issn><eissn>1879-3460</eissn><abstract>Potatoes contain several nutrients essential for fungal growth, making them an excellent component of media such as the popular Potato Dextrose Agar (PDA) medium. 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| source | Elsevier ScienceDirect Journals Complete |
| subjects | Dextrose Fusarium High-performance liquid chromatography Liquid chromatography Metabolites Mycotoxins Nutrients Potato dextrose agar Potatoes Regulation Secondary metabolites Solanine Statistical analysis |
| title | The effects of different potato dextrose agar media on secondary metabolite production in Fusarium |
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