The alarming association between antibiotic resistance and reduced susceptibility to biocides in nosocomial MRSA isolates from two regional hospitals in Egypt
Methicillin-resistant Staphylococcus aureus is one of the major clinical problems in hospitals because of its resistance to many antimicrobials. Biocides are used in hospitals to control nosocomial infections. This work aimed to investigate the relationship between the presence of integrons and redu...
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| Veröffentlicht in: | Archives of microbiology 2021-08, Vol.203 (6), p.3295-3303 |
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| creator | Youssef, Christiana R. B. Kadry, Ashraf A. Shaker, Ghada H. El-Ganiny, Amira M. |
| description | Methicillin-resistant
Staphylococcus aureus
is one of the major clinical problems in hospitals because of its resistance to many antimicrobials. Biocides are used in hospitals to control nosocomial infections. This work aimed to investigate the relationship between the presence of integrons and reduced susceptibility to both biocides and antimicrobials in nosocomial multidrug-resistant (MDR)-MRSA isolates. A total of 114 clinical and eight environmental MRSA isolates were collected from Zagazig University Hospitals and El-Ahrar Educational Hospital, Egypt. These isolates were identified as MRSA by disk diffusion method (DDM) and confirmed by PCR. Susceptibility profile against 12 antibiotics and five biocides was determined by DDM and agar dilution method, respectively. Presence of integrons was investigated by PCR in MDR isolates. Seventy-five clinical and six environmental isolates were MDR and had reduced susceptibility to biocides. Class I integron was detected in plasmid DNA of 34 isolates and genomic DNA of 14 isolates. Meanwhile, class II integron was only detected in plasmid DNA of 10 clinical isolates. This study revealed a high prevalence of MDR-MRSA clinical and environmental isolates, both had reduced susceptibility to investigated biocides. Class I integron was more predominant in plasmid DNA of isolates, indicating that plasmid is a major carrier for integrons that transfer resistance genes
.
In conclusion, the association between antibiotic resistance and biocides reduced susceptibility is alarming. The selection of curative antibiotic should depend on the antimicrobial susceptibility profile. Furthermore, biocides should always be used at appropriate concentrations to prevent the evolution of resistance and to control the hospital-transmission of MRSA. |
| doi_str_mv | 10.1007/s00203-021-02314-6 |
| format | Article |
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Staphylococcus aureus
is one of the major clinical problems in hospitals because of its resistance to many antimicrobials. Biocides are used in hospitals to control nosocomial infections. This work aimed to investigate the relationship between the presence of integrons and reduced susceptibility to both biocides and antimicrobials in nosocomial multidrug-resistant (MDR)-MRSA isolates. A total of 114 clinical and eight environmental MRSA isolates were collected from Zagazig University Hospitals and El-Ahrar Educational Hospital, Egypt. These isolates were identified as MRSA by disk diffusion method (DDM) and confirmed by PCR. Susceptibility profile against 12 antibiotics and five biocides was determined by DDM and agar dilution method, respectively. Presence of integrons was investigated by PCR in MDR isolates. Seventy-five clinical and six environmental isolates were MDR and had reduced susceptibility to biocides. Class I integron was detected in plasmid DNA of 34 isolates and genomic DNA of 14 isolates. Meanwhile, class II integron was only detected in plasmid DNA of 10 clinical isolates. This study revealed a high prevalence of MDR-MRSA clinical and environmental isolates, both had reduced susceptibility to investigated biocides. Class I integron was more predominant in plasmid DNA of isolates, indicating that plasmid is a major carrier for integrons that transfer resistance genes
.
In conclusion, the association between antibiotic resistance and biocides reduced susceptibility is alarming. The selection of curative antibiotic should depend on the antimicrobial susceptibility profile. Furthermore, biocides should always be used at appropriate concentrations to prevent the evolution of resistance and to control the hospital-transmission of MRSA.</description><identifier>ISSN: 0302-8933</identifier><identifier>EISSN: 1432-072X</identifier><identifier>DOI: 10.1007/s00203-021-02314-6</identifier><identifier>PMID: 33864112</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Antibiotic resistance ; Antibiotics ; Antiinfectives and antibacterials ; Antimicrobial agents ; Biochemistry ; Biocides ; Biomedical and Life Sciences ; Biotechnology ; Cell Biology ; Clinical isolates ; Deoxyribonucleic acid ; Dilution ; DNA ; Drug resistance ; Ecology ; Hospitals ; Life Sciences ; Methicillin ; Microbial Ecology ; Microbiology ; Multidrug resistance ; Nosocomial infection ; Original Paper ; Plasmids ; Polymerase chain reaction ; Public health ; Staphylococcus aureus ; Staphylococcus infections ; Susceptibility</subject><ispartof>Archives of microbiology, 2021-08, Vol.203 (6), p.3295-3303</ispartof><rights>The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2021</rights><rights>The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2021.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c375t-2ceaf7397a513bb680f8351dd56f036d3f42e57a543eae8da18872e3f671fd073</citedby><cites>FETCH-LOGICAL-c375t-2ceaf7397a513bb680f8351dd56f036d3f42e57a543eae8da18872e3f671fd073</cites><orcidid>0000-0002-2694-1998</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00203-021-02314-6$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00203-021-02314-6$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33864112$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Youssef, Christiana R. B.</creatorcontrib><creatorcontrib>Kadry, Ashraf A.</creatorcontrib><creatorcontrib>Shaker, Ghada H.</creatorcontrib><creatorcontrib>El-Ganiny, Amira M.</creatorcontrib><title>The alarming association between antibiotic resistance and reduced susceptibility to biocides in nosocomial MRSA isolates from two regional hospitals in Egypt</title><title>Archives of microbiology</title><addtitle>Arch Microbiol</addtitle><addtitle>Arch Microbiol</addtitle><description>Methicillin-resistant
Staphylococcus aureus
is one of the major clinical problems in hospitals because of its resistance to many antimicrobials. Biocides are used in hospitals to control nosocomial infections. This work aimed to investigate the relationship between the presence of integrons and reduced susceptibility to both biocides and antimicrobials in nosocomial multidrug-resistant (MDR)-MRSA isolates. A total of 114 clinical and eight environmental MRSA isolates were collected from Zagazig University Hospitals and El-Ahrar Educational Hospital, Egypt. These isolates were identified as MRSA by disk diffusion method (DDM) and confirmed by PCR. Susceptibility profile against 12 antibiotics and five biocides was determined by DDM and agar dilution method, respectively. Presence of integrons was investigated by PCR in MDR isolates. Seventy-five clinical and six environmental isolates were MDR and had reduced susceptibility to biocides. Class I integron was detected in plasmid DNA of 34 isolates and genomic DNA of 14 isolates. Meanwhile, class II integron was only detected in plasmid DNA of 10 clinical isolates. This study revealed a high prevalence of MDR-MRSA clinical and environmental isolates, both had reduced susceptibility to investigated biocides. Class I integron was more predominant in plasmid DNA of isolates, indicating that plasmid is a major carrier for integrons that transfer resistance genes
.
In conclusion, the association between antibiotic resistance and biocides reduced susceptibility is alarming. The selection of curative antibiotic should depend on the antimicrobial susceptibility profile. Furthermore, biocides should always be used at appropriate concentrations to prevent the evolution of resistance and to control the hospital-transmission of MRSA.</description><subject>Antibiotic resistance</subject><subject>Antibiotics</subject><subject>Antiinfectives and antibacterials</subject><subject>Antimicrobial agents</subject><subject>Biochemistry</subject><subject>Biocides</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>Cell Biology</subject><subject>Clinical isolates</subject><subject>Deoxyribonucleic acid</subject><subject>Dilution</subject><subject>DNA</subject><subject>Drug resistance</subject><subject>Ecology</subject><subject>Hospitals</subject><subject>Life Sciences</subject><subject>Methicillin</subject><subject>Microbial Ecology</subject><subject>Microbiology</subject><subject>Multidrug resistance</subject><subject>Nosocomial infection</subject><subject>Original Paper</subject><subject>Plasmids</subject><subject>Polymerase chain reaction</subject><subject>Public health</subject><subject>Staphylococcus aureus</subject><subject>Staphylococcus infections</subject><subject>Susceptibility</subject><issn>0302-8933</issn><issn>1432-072X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kUFrFTEUhYMo9ln9Ay4k4MbNaJI7mclbltKqUBG0grshM7nzmjKTjEmG8v6Mv9X7-qqCCxchXM53zk04jL2U4q0Uon2XhVACKqEkHZB11TxiG1mDqkSrvj9mGwFCVWYLcMKe5XwrhFTGmKfsBMA0tZRqw35e3yC3k02zDztuc46Dt8XHwHssd4iB21B872PxA0-YfS42DGQJjka3Duh4XvOAy4GafNnzEjnxg3eYuQ88RMqMs7cT__Tl6xn3OU62kDamOPNyFylnRwtJv4l58cVO976L3X4pz9mTkWZ88XCfsm-XF9fnH6qrz-8_np9dVQO0ulRqQDu2sG2tltD3jRGjAS2d080ooHEw1go1qTWgReOsNKZVCGPTytGJFk7Zm2PukuKPFXPpZk-fmiYbMK65U1rWjWhroQl9_Q96G9dEzz9QGqTStd4SpY7UkGLOCcduSX62ad9J0R3a647tddRed99e15Dp1UP02s_o_lh-10UAHIFMUthh-rv7P7G_AH2rqAo</recordid><startdate>20210801</startdate><enddate>20210801</enddate><creator>Youssef, Christiana R. B.</creator><creator>Kadry, Ashraf A.</creator><creator>Shaker, Ghada H.</creator><creator>El-Ganiny, Amira M.</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-2694-1998</orcidid></search><sort><creationdate>20210801</creationdate><title>The alarming association between antibiotic resistance and reduced susceptibility to biocides in nosocomial MRSA isolates from two regional hospitals in Egypt</title><author>Youssef, Christiana R. B. ; Kadry, Ashraf A. ; Shaker, Ghada H. ; El-Ganiny, Amira M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c375t-2ceaf7397a513bb680f8351dd56f036d3f42e57a543eae8da18872e3f671fd073</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Antibiotic resistance</topic><topic>Antibiotics</topic><topic>Antiinfectives and antibacterials</topic><topic>Antimicrobial agents</topic><topic>Biochemistry</topic><topic>Biocides</topic><topic>Biomedical and Life Sciences</topic><topic>Biotechnology</topic><topic>Cell Biology</topic><topic>Clinical isolates</topic><topic>Deoxyribonucleic acid</topic><topic>Dilution</topic><topic>DNA</topic><topic>Drug resistance</topic><topic>Ecology</topic><topic>Hospitals</topic><topic>Life Sciences</topic><topic>Methicillin</topic><topic>Microbial Ecology</topic><topic>Microbiology</topic><topic>Multidrug resistance</topic><topic>Nosocomial infection</topic><topic>Original Paper</topic><topic>Plasmids</topic><topic>Polymerase chain reaction</topic><topic>Public health</topic><topic>Staphylococcus aureus</topic><topic>Staphylococcus infections</topic><topic>Susceptibility</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Youssef, Christiana R. 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B.</au><au>Kadry, Ashraf A.</au><au>Shaker, Ghada H.</au><au>El-Ganiny, Amira M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The alarming association between antibiotic resistance and reduced susceptibility to biocides in nosocomial MRSA isolates from two regional hospitals in Egypt</atitle><jtitle>Archives of microbiology</jtitle><stitle>Arch Microbiol</stitle><addtitle>Arch Microbiol</addtitle><date>2021-08-01</date><risdate>2021</risdate><volume>203</volume><issue>6</issue><spage>3295</spage><epage>3303</epage><pages>3295-3303</pages><issn>0302-8933</issn><eissn>1432-072X</eissn><abstract>Methicillin-resistant
Staphylococcus aureus
is one of the major clinical problems in hospitals because of its resistance to many antimicrobials. Biocides are used in hospitals to control nosocomial infections. This work aimed to investigate the relationship between the presence of integrons and reduced susceptibility to both biocides and antimicrobials in nosocomial multidrug-resistant (MDR)-MRSA isolates. A total of 114 clinical and eight environmental MRSA isolates were collected from Zagazig University Hospitals and El-Ahrar Educational Hospital, Egypt. These isolates were identified as MRSA by disk diffusion method (DDM) and confirmed by PCR. Susceptibility profile against 12 antibiotics and five biocides was determined by DDM and agar dilution method, respectively. Presence of integrons was investigated by PCR in MDR isolates. Seventy-five clinical and six environmental isolates were MDR and had reduced susceptibility to biocides. Class I integron was detected in plasmid DNA of 34 isolates and genomic DNA of 14 isolates. Meanwhile, class II integron was only detected in plasmid DNA of 10 clinical isolates. This study revealed a high prevalence of MDR-MRSA clinical and environmental isolates, both had reduced susceptibility to investigated biocides. Class I integron was more predominant in plasmid DNA of isolates, indicating that plasmid is a major carrier for integrons that transfer resistance genes
.
In conclusion, the association between antibiotic resistance and biocides reduced susceptibility is alarming. The selection of curative antibiotic should depend on the antimicrobial susceptibility profile. Furthermore, biocides should always be used at appropriate concentrations to prevent the evolution of resistance and to control the hospital-transmission of MRSA.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>33864112</pmid><doi>10.1007/s00203-021-02314-6</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0002-2694-1998</orcidid></addata></record> |
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| subjects | Antibiotic resistance Antibiotics Antiinfectives and antibacterials Antimicrobial agents Biochemistry Biocides Biomedical and Life Sciences Biotechnology Cell Biology Clinical isolates Deoxyribonucleic acid Dilution DNA Drug resistance Ecology Hospitals Life Sciences Methicillin Microbial Ecology Microbiology Multidrug resistance Nosocomial infection Original Paper Plasmids Polymerase chain reaction Public health Staphylococcus aureus Staphylococcus infections Susceptibility |
| title | The alarming association between antibiotic resistance and reduced susceptibility to biocides in nosocomial MRSA isolates from two regional hospitals in Egypt |
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