Preparation of Artificial Hexaplex Composed of Non‐Natural Nucleic Acid

This article describes synthetic procedures for acyclic D‐threoninol nucleic acid tethering of bifacial nucleobases. Because these nucleobases have complementary hydrogen bonding sites on both sides, their oligomers can form a hexaplex. These hexaplexes are suitable for use as metal or pH sensors and as supramolecular motifs. © 2021 Wiley Periodicals LLC. Basic Protocol 1: Synthesis of D‐threoninol backbone Basic Protocol 2: Synthesis of D‐aTNA tethering cyanuric acid Basic Protocol 3: Synthesis of D‐aTNA tethering a 2‐aminopyrimidine moiety Basic Protocol 4: Synthesis of D‐aTNA tethering a 2,4,6‐triaminopyrimidine moiety Basic Protocol 5: Synthesis of D‐aTNA oligomer tethering cyanuric acid, 2‐aminopyrimidine, or 2,4,6‐triaminopyrimidine