Gene expression of bovine endometrial epithelial cells cultured in matrigel
Glandular epithelial cells (GE) in the endometrium are thought to support the elongation and survival of ruminant embryos by secreting histotrophs. In the present study, the gene expression of bovine endometrial epithelial cells cultured in matrigel was analyzed and examined whether it could be an i...
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| Veröffentlicht in: | Cell and tissue research 2021-07, Vol.385 (1), p.265-275 |
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| creator | Nishino, Daichi Kotake, Ai Yun, Chi Sun Rahman, Al-Nur Md. Iftekhar El-Sharawy, Mohamed Yamanaka, Ken-ichi Khandoker, M. A. M. Yahia Yamauchi, Nobuhiko |
| description | Glandular epithelial cells (GE) in the endometrium are thought to support the elongation and survival of ruminant embryos by secreting histotrophs. In the present study, the gene expression of bovine endometrial epithelial cells cultured in matrigel was analyzed and examined whether it could be an in vitro model of GE. Bovine endometrial epithelial cells (BEE) and stromal cells (BES) were isolated from the slaughterhouse uteri and cultured in DMEM/F12 + 10% FBS. BEE showed the gland-like structure morphological changes when cultured in 15% matrigel but could not be identified in higher concentrations of the matrigel (30% or 60%). The expression of typical genes expressed in GE,
SERPINA14
and
GRP
, was substantially high in matrigel-cultured BEE than in monolayer (
P
|
| doi_str_mv | 10.1007/s00441-021-03418-7 |
| format | Article |
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SERPINA14
and
GRP
, was substantially high in matrigel-cultured BEE than in monolayer (
P
< 0.05). P4 and INFα have no significant effect on the
SERPINA14
expression of BEE cultured in matrigel without co-culture with BES. On the other hand, when BEE were co-cultured with BES in matrigel culture, the expression of
FGF13
was increased by the P4 treatment (
P
< 0.05). Furthermore,
SERPINA14
and
TXN
expressions were increased by P4 + IFNα treatment (
P
< 0.05). These results demonstrate the appropriate conditions for BEE to form glandular structures in matrigel and the effect of co-culture with BES. The present study highlighted the possible use of matrigel for the culture of BEE to investigate the expression of cell-specific glandular epithelial genes as well as P4 and type-I IFN as factors controlling endometrial function during the implantation period.</description><identifier>ISSN: 0302-766X</identifier><identifier>EISSN: 1432-0878</identifier><identifier>DOI: 10.1007/s00441-021-03418-7</identifier><identifier>PMID: 33837849</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Abattoirs ; Animals ; Biocompatible Materials - therapeutic use ; Biomedical and Life Sciences ; Biomedicine ; Cattle ; Cell culture ; Cells, Cultured ; Collagen - therapeutic use ; Drug Combinations ; Embryonic development ; Embryos ; Endometrium ; Endometrium - physiopathology ; Epithelial cells ; Epithelial Cells - metabolism ; Ethylenediaminetetraacetic acid ; Female ; Gene expression ; Gene Expression - genetics ; Genes ; Human Genetics ; Laminin - therapeutic use ; Molecular Medicine ; Proteoglycans - therapeutic use ; Proteomics ; Regular Article ; Stromal cells ; α-Interferon</subject><ispartof>Cell and tissue research, 2021-07, Vol.385 (1), p.265-275</ispartof><rights>The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2021</rights><rights>2021. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.</rights><rights>COPYRIGHT 2021 Springer</rights><rights>The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2021.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c473t-d41f981e83e7c11840d86dfc318446ca1f4032222f73a43d8eed2f77cb690d553</citedby><cites>FETCH-LOGICAL-c473t-d41f981e83e7c11840d86dfc318446ca1f4032222f73a43d8eed2f77cb690d553</cites><orcidid>0000-0001-9254-2488</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00441-021-03418-7$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00441-021-03418-7$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33837849$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nishino, Daichi</creatorcontrib><creatorcontrib>Kotake, Ai</creatorcontrib><creatorcontrib>Yun, Chi Sun</creatorcontrib><creatorcontrib>Rahman, Al-Nur Md. Iftekhar</creatorcontrib><creatorcontrib>El-Sharawy, Mohamed</creatorcontrib><creatorcontrib>Yamanaka, Ken-ichi</creatorcontrib><creatorcontrib>Khandoker, M. A. M. Yahia</creatorcontrib><creatorcontrib>Yamauchi, Nobuhiko</creatorcontrib><title>Gene expression of bovine endometrial epithelial cells cultured in matrigel</title><title>Cell and tissue research</title><addtitle>Cell Tissue Res</addtitle><addtitle>Cell Tissue Res</addtitle><description>Glandular epithelial cells (GE) in the endometrium are thought to support the elongation and survival of ruminant embryos by secreting histotrophs. In the present study, the gene expression of bovine endometrial epithelial cells cultured in matrigel was analyzed and examined whether it could be an in vitro model of GE. Bovine endometrial epithelial cells (BEE) and stromal cells (BES) were isolated from the slaughterhouse uteri and cultured in DMEM/F12 + 10% FBS. BEE showed the gland-like structure morphological changes when cultured in 15% matrigel but could not be identified in higher concentrations of the matrigel (30% or 60%). The expression of typical genes expressed in GE,
SERPINA14
and
GRP
, was substantially high in matrigel-cultured BEE than in monolayer (
P
< 0.05). P4 and INFα have no significant effect on the
SERPINA14
expression of BEE cultured in matrigel without co-culture with BES. On the other hand, when BEE were co-cultured with BES in matrigel culture, the expression of
FGF13
was increased by the P4 treatment (
P
< 0.05). Furthermore,
SERPINA14
and
TXN
expressions were increased by P4 + IFNα treatment (
P
< 0.05). These results demonstrate the appropriate conditions for BEE to form glandular structures in matrigel and the effect of co-culture with BES. The present study highlighted the possible use of matrigel for the culture of BEE to investigate the expression of cell-specific glandular epithelial genes as well as P4 and type-I IFN as factors controlling endometrial function during the implantation period.</description><subject>Abattoirs</subject><subject>Animals</subject><subject>Biocompatible Materials - therapeutic use</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Cattle</subject><subject>Cell culture</subject><subject>Cells, Cultured</subject><subject>Collagen - therapeutic use</subject><subject>Drug Combinations</subject><subject>Embryonic development</subject><subject>Embryos</subject><subject>Endometrium</subject><subject>Endometrium - physiopathology</subject><subject>Epithelial cells</subject><subject>Epithelial Cells - metabolism</subject><subject>Ethylenediaminetetraacetic acid</subject><subject>Female</subject><subject>Gene expression</subject><subject>Gene Expression - genetics</subject><subject>Genes</subject><subject>Human Genetics</subject><subject>Laminin - therapeutic use</subject><subject>Molecular Medicine</subject><subject>Proteoglycans - therapeutic use</subject><subject>Proteomics</subject><subject>Regular Article</subject><subject>Stromal cells</subject><subject>α-Interferon</subject><issn>0302-766X</issn><issn>1432-0878</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp9kV1rFDEUhoModq3-AS9kQBBvpuZrk8xlKbaKBW8UvAvZ5GQ3JZOsyUzRf99Mt1orYkLI4eR5D-fkReglwScEY_muYsw56TFth3GievkIrQhntMdKqsdohRmmvRTi2xF6VusVxoQLMTxFR4wpJhUfVujTBSTo4Me-QK0hpy77bpOvw5JMLo8wlWBiB_sw7SAuoYUYa2fnOM0FXBdSN5oGbSE-R0-8iRVe3N3H6Ov5-y9nH_rLzxcfz04ve8slm3rHiR8UAcVAWkIUx04J5y1rIRfWEM8xo215yQxnTgG4Fku7EQN26zU7Rm8Pdfclf5-hTnoMdWnLJMhz1XRNCGVS3qKv_0Kv8lxS665RfFBsTTG9p7Ymgg7J56kYuxTVp0IoLMkgcKNO_kG17WAMNifwoeUfCN78IdiBidOu5jhP7Z_rQ5AeQFtyrQW83pcwmvJTE6wXq_XBat2s1rdWa9lEr-5GmzcjuN-SX942gB2A2p7SFsr97P8pewMP_rCm</recordid><startdate>20210701</startdate><enddate>20210701</enddate><creator>Nishino, Daichi</creator><creator>Kotake, Ai</creator><creator>Yun, Chi Sun</creator><creator>Rahman, Al-Nur Md. Iftekhar</creator><creator>El-Sharawy, Mohamed</creator><creator>Yamanaka, Ken-ichi</creator><creator>Khandoker, M. A. M. Yahia</creator><creator>Yamauchi, Nobuhiko</creator><general>Springer Berlin Heidelberg</general><general>Springer</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QP</scope><scope>7QR</scope><scope>7RV</scope><scope>7SS</scope><scope>7TK</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>NAPCQ</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-9254-2488</orcidid></search><sort><creationdate>20210701</creationdate><title>Gene expression of bovine endometrial epithelial cells cultured in matrigel</title><author>Nishino, Daichi ; Kotake, Ai ; Yun, Chi Sun ; Rahman, Al-Nur Md. Iftekhar ; El-Sharawy, Mohamed ; Yamanaka, Ken-ichi ; Khandoker, M. A. M. Yahia ; Yamauchi, Nobuhiko</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c473t-d41f981e83e7c11840d86dfc318446ca1f4032222f73a43d8eed2f77cb690d553</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Abattoirs</topic><topic>Animals</topic><topic>Biocompatible Materials - therapeutic use</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Cattle</topic><topic>Cell culture</topic><topic>Cells, Cultured</topic><topic>Collagen - therapeutic use</topic><topic>Drug Combinations</topic><topic>Embryonic development</topic><topic>Embryos</topic><topic>Endometrium</topic><topic>Endometrium - physiopathology</topic><topic>Epithelial cells</topic><topic>Epithelial Cells - metabolism</topic><topic>Ethylenediaminetetraacetic acid</topic><topic>Female</topic><topic>Gene expression</topic><topic>Gene Expression - genetics</topic><topic>Genes</topic><topic>Human Genetics</topic><topic>Laminin - therapeutic use</topic><topic>Molecular Medicine</topic><topic>Proteoglycans - therapeutic use</topic><topic>Proteomics</topic><topic>Regular Article</topic><topic>Stromal cells</topic><topic>α-Interferon</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nishino, Daichi</creatorcontrib><creatorcontrib>Kotake, Ai</creatorcontrib><creatorcontrib>Yun, Chi Sun</creatorcontrib><creatorcontrib>Rahman, Al-Nur Md. Iftekhar</creatorcontrib><creatorcontrib>El-Sharawy, Mohamed</creatorcontrib><creatorcontrib>Yamanaka, Ken-ichi</creatorcontrib><creatorcontrib>Khandoker, M. A. M. Yahia</creatorcontrib><creatorcontrib>Yamauchi, Nobuhiko</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Neurosciences Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Cell and tissue research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nishino, Daichi</au><au>Kotake, Ai</au><au>Yun, Chi Sun</au><au>Rahman, Al-Nur Md. Iftekhar</au><au>El-Sharawy, Mohamed</au><au>Yamanaka, Ken-ichi</au><au>Khandoker, M. A. M. Yahia</au><au>Yamauchi, Nobuhiko</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Gene expression of bovine endometrial epithelial cells cultured in matrigel</atitle><jtitle>Cell and tissue research</jtitle><stitle>Cell Tissue Res</stitle><addtitle>Cell Tissue Res</addtitle><date>2021-07-01</date><risdate>2021</risdate><volume>385</volume><issue>1</issue><spage>265</spage><epage>275</epage><pages>265-275</pages><issn>0302-766X</issn><eissn>1432-0878</eissn><abstract>Glandular epithelial cells (GE) in the endometrium are thought to support the elongation and survival of ruminant embryos by secreting histotrophs. In the present study, the gene expression of bovine endometrial epithelial cells cultured in matrigel was analyzed and examined whether it could be an in vitro model of GE. Bovine endometrial epithelial cells (BEE) and stromal cells (BES) were isolated from the slaughterhouse uteri and cultured in DMEM/F12 + 10% FBS. BEE showed the gland-like structure morphological changes when cultured in 15% matrigel but could not be identified in higher concentrations of the matrigel (30% or 60%). The expression of typical genes expressed in GE,
SERPINA14
and
GRP
, was substantially high in matrigel-cultured BEE than in monolayer (
P
< 0.05). P4 and INFα have no significant effect on the
SERPINA14
expression of BEE cultured in matrigel without co-culture with BES. On the other hand, when BEE were co-cultured with BES in matrigel culture, the expression of
FGF13
was increased by the P4 treatment (
P
< 0.05). Furthermore,
SERPINA14
and
TXN
expressions were increased by P4 + IFNα treatment (
P
< 0.05). These results demonstrate the appropriate conditions for BEE to form glandular structures in matrigel and the effect of co-culture with BES. The present study highlighted the possible use of matrigel for the culture of BEE to investigate the expression of cell-specific glandular epithelial genes as well as P4 and type-I IFN as factors controlling endometrial function during the implantation period.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>33837849</pmid><doi>10.1007/s00441-021-03418-7</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0001-9254-2488</orcidid></addata></record> |
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| ispartof | Cell and tissue research, 2021-07, Vol.385 (1), p.265-275 |
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| subjects | Abattoirs Animals Biocompatible Materials - therapeutic use Biomedical and Life Sciences Biomedicine Cattle Cell culture Cells, Cultured Collagen - therapeutic use Drug Combinations Embryonic development Embryos Endometrium Endometrium - physiopathology Epithelial cells Epithelial Cells - metabolism Ethylenediaminetetraacetic acid Female Gene expression Gene Expression - genetics Genes Human Genetics Laminin - therapeutic use Molecular Medicine Proteoglycans - therapeutic use Proteomics Regular Article Stromal cells α-Interferon |
| title | Gene expression of bovine endometrial epithelial cells cultured in matrigel |
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