Electrochemical Impedance Spectroscopy as a Tool for Monitoring Cell Differentiation from Floor Plate Progenitors to Midbrain Neurons in Real Time
Here shows that electrical impedance spectroscopy can be used as a non‐invasive and real time tool to probe cell adhesion and differentiation from midbrain floor plate progenitors into midbrain neurons on Au electrodes coated with human laminin. The electrical data and equivalent circuit modeling ar...
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| Veröffentlicht in: | Advanced biology 2021-06, Vol.5 (6), p.e2100330-n/a |
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| creator | Elghajiji, Aya Wang, Xin Weston, Stephen D. Zeck, Guenther Hengerer, Bastian Tosh, David Rocha, Paulo R. F. |
| description | Here shows that electrical impedance spectroscopy can be used as a non‐invasive and real time tool to probe cell adhesion and differentiation from midbrain floor plate progenitors into midbrain neurons on Au electrodes coated with human laminin. The electrical data and equivalent circuit modeling are consistent with standard microscopy analysis and reveal that within the first 6 hours progenitor cells sediment and attach to the electrode within 40 hours. Between 40 and 120 hours, midbrain progenitor cells differentiate into midbrain neurons, followed by an electrochemically stable maturation phase. The ability to sense and characterize non‐invasively and in real time cell differentiation opens up unprecedented avenues for implantable therapies and differentiation strategies.
Here, it is shown that electrical impedance spectroscopy can be used as a non‐invasive and real time tool to probe cell adhesion and differentiation from midbrain floor plate progenitors into midbrain neurons on Au electrodes coated with human laminin. The ability to sense and characterize non‐invasively and in real time, cell differentiation opens avenues for implantable therapies and differentiation strategies. |
| doi_str_mv | 10.1002/adbi.202100330 |
| format | Article |
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Here, it is shown that electrical impedance spectroscopy can be used as a non‐invasive and real time tool to probe cell adhesion and differentiation from midbrain floor plate progenitors into midbrain neurons on Au electrodes coated with human laminin. The ability to sense and characterize non‐invasively and in real time, cell differentiation opens avenues for implantable therapies and differentiation strategies.</description><identifier>ISSN: 2701-0198</identifier><identifier>EISSN: 2701-0198</identifier><identifier>DOI: 10.1002/adbi.202100330</identifier><identifier>PMID: 33825335</identifier><language>eng</language><subject>bioelectronics ; biophysics ; cell differentiation ; electrochemical impedance spectroscopy ; spinal cord injuries</subject><ispartof>Advanced biology, 2021-06, Vol.5 (6), p.e2100330-n/a</ispartof><rights>2021 The Authors. Advanced Biology published by Wiley‐VCH GmbH</rights><rights>2021 The Authors. Advanced Biology published by Wiley-VCH GmbH.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4510-155a774a70870e9053147f76259090c7c89780a80eea282a20facf8134162dd53</citedby><cites>FETCH-LOGICAL-c4510-155a774a70870e9053147f76259090c7c89780a80eea282a20facf8134162dd53</cites><orcidid>0000-0002-8917-9101</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fadbi.202100330$$EPDF$$P50$$Gwiley$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fadbi.202100330$$EHTML$$P50$$Gwiley$$Hfree_for_read</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33825335$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Elghajiji, Aya</creatorcontrib><creatorcontrib>Wang, Xin</creatorcontrib><creatorcontrib>Weston, Stephen D.</creatorcontrib><creatorcontrib>Zeck, Guenther</creatorcontrib><creatorcontrib>Hengerer, Bastian</creatorcontrib><creatorcontrib>Tosh, David</creatorcontrib><creatorcontrib>Rocha, Paulo R. F.</creatorcontrib><title>Electrochemical Impedance Spectroscopy as a Tool for Monitoring Cell Differentiation from Floor Plate Progenitors to Midbrain Neurons in Real Time</title><title>Advanced biology</title><addtitle>Adv Biol (Weinh)</addtitle><description>Here shows that electrical impedance spectroscopy can be used as a non‐invasive and real time tool to probe cell adhesion and differentiation from midbrain floor plate progenitors into midbrain neurons on Au electrodes coated with human laminin. The electrical data and equivalent circuit modeling are consistent with standard microscopy analysis and reveal that within the first 6 hours progenitor cells sediment and attach to the electrode within 40 hours. Between 40 and 120 hours, midbrain progenitor cells differentiate into midbrain neurons, followed by an electrochemically stable maturation phase. The ability to sense and characterize non‐invasively and in real time cell differentiation opens up unprecedented avenues for implantable therapies and differentiation strategies.
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Here, it is shown that electrical impedance spectroscopy can be used as a non‐invasive and real time tool to probe cell adhesion and differentiation from midbrain floor plate progenitors into midbrain neurons on Au electrodes coated with human laminin. The ability to sense and characterize non‐invasively and in real time, cell differentiation opens avenues for implantable therapies and differentiation strategies.</abstract><pmid>33825335</pmid><doi>10.1002/adbi.202100330</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0002-8917-9101</orcidid><oa>free_for_read</oa></addata></record> |
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| source | Wiley Online Library Journals Frontfile Complete |
| subjects | bioelectronics biophysics cell differentiation electrochemical impedance spectroscopy spinal cord injuries |
| title | Electrochemical Impedance Spectroscopy as a Tool for Monitoring Cell Differentiation from Floor Plate Progenitors to Midbrain Neurons in Real Time |
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