Renal claudin-14 expression is not required for regulating Mg2+ balance in mice

The kidneys play a crucial role in maintaining Ca2+ and Mg2+ homeostasis by regulating these minerals' reabsorption. In the thick ascending limb of Henle's loop (TAL), Ca2+ and Mg2+ are reabsorbed through the tight junctions by a shared paracellular pathway formed by claudin-16 and claudin...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:American journal of physiology. Renal physiology 2021-05, Vol.320 (5), p.F897-F907
Hauptverfasser: Ferreira, Patrícia G, van Megen, Wouter H, Tan, Rebecca, L Lee, Christy H, Svenningsen, Per, Alexander, R Todd, Dimke, Henrik
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page F907
container_issue 5
container_start_page F897
container_title American journal of physiology. Renal physiology
container_volume 320
creator Ferreira, Patrícia G
van Megen, Wouter H
Tan, Rebecca
L Lee, Christy H
Svenningsen, Per
Alexander, R Todd
Dimke, Henrik
description The kidneys play a crucial role in maintaining Ca2+ and Mg2+ homeostasis by regulating these minerals' reabsorption. In the thick ascending limb of Henle's loop (TAL), Ca2+ and Mg2+ are reabsorbed through the tight junctions by a shared paracellular pathway formed by claudin-16 and claudin-19. Hypercalcemia activates the Ca2+-sensing receptor (CaSR) in the TAL, causing upregulation of pore-blocking claudin-14 (CLDN14), which reduces Ca2+ and Mg2+ reabsorption from this segment. In addition, a high-Mg2+ diet is known to increase both urinary Mg2+ and Ca2+ excretion. Since Mg2+ may also activate CaSR, we aimed to investigate whether CaSR-dependent increases in CLDN14 expression also regulate urinary Mg2+ excretion in response to hypermagnesemia. Here, we show that a Mg2+-enriched diet increased urinary Mg2+ and Ca2+ excretion in mice; however, this occurred without detectable changes in renal CLDN14 expression. The administration of a high-Mg2+ diet to Cldn14−/− mice did not cause more pronounced hypermagnesemia or significantly alter urinary Mg2+ excretion. Finally, in vitro evaluation of CaSR-driven Cldn14 promoter activity in response to increasing Mg2+ concentrations revealed that Cldn14 expression only increases at supraphysiological extracellular Mg2+ levels. Together, these results suggest that CLDN14 is not involved in regulating extracellular Mg2+ balance following high dietary Mg2+ intake.
doi_str_mv 10.1152/ajprenal.00590.2020
format Article
fullrecord <record><control><sourceid>proquest</sourceid><recordid>TN_cdi_proquest_miscellaneous_2508892477</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2508892477</sourcerecordid><originalsourceid>FETCH-LOGICAL-p261t-7cebbf68f3a78069907e23b693fe77051a7227ff0ac2eaf647a781e4537ce0e93</originalsourceid><addsrcrecordid>eNpdj09LxDAQxYMouK5-Ai8BL4K0TiZt0hxl8R-sLIiCtyXtTkqWbrrbtODHN4uePM0M83vvzTB2LSAXosR7u90PFGyXA5QGcgSEEzZLG8xEodRp6o0UWVXqr3N2EeMWAIRAMWOr96OON52dNj4kmtN38orR94H7yEM_8oEOkx9ow10_pKGdOjv60PK3Fu94bTsbGuI-8J1v6JKdOdtFuvqrc_b59PixeMmWq-fXxcMy26MSY6YbqmunKietrkAZA5pQ1spIR1pDKaxG1M6BbZCsU4VOnKCilEkJZOSc3f767of-MFEc1zsfG-rSMdRPcY0lVJXBQuuE3vxDt_00pK-PlExZgIjyB_bPX5A</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2530510222</pqid></control><display><type>article</type><title>Renal claudin-14 expression is not required for regulating Mg2+ balance in mice</title><source>American Physiological Society</source><source>EZB-FREE-00999 freely available EZB journals</source><source>Alma/SFX Local Collection</source><creator>Ferreira, Patrícia G ; van Megen, Wouter H ; Tan, Rebecca ; L Lee, Christy H ; Svenningsen, Per ; Alexander, R Todd ; Dimke, Henrik</creator><creatorcontrib>Ferreira, Patrícia G ; van Megen, Wouter H ; Tan, Rebecca ; L Lee, Christy H ; Svenningsen, Per ; Alexander, R Todd ; Dimke, Henrik</creatorcontrib><description>The kidneys play a crucial role in maintaining Ca2+ and Mg2+ homeostasis by regulating these minerals' reabsorption. In the thick ascending limb of Henle's loop (TAL), Ca2+ and Mg2+ are reabsorbed through the tight junctions by a shared paracellular pathway formed by claudin-16 and claudin-19. Hypercalcemia activates the Ca2+-sensing receptor (CaSR) in the TAL, causing upregulation of pore-blocking claudin-14 (CLDN14), which reduces Ca2+ and Mg2+ reabsorption from this segment. In addition, a high-Mg2+ diet is known to increase both urinary Mg2+ and Ca2+ excretion. Since Mg2+ may also activate CaSR, we aimed to investigate whether CaSR-dependent increases in CLDN14 expression also regulate urinary Mg2+ excretion in response to hypermagnesemia. Here, we show that a Mg2+-enriched diet increased urinary Mg2+ and Ca2+ excretion in mice; however, this occurred without detectable changes in renal CLDN14 expression. The administration of a high-Mg2+ diet to Cldn14−/− mice did not cause more pronounced hypermagnesemia or significantly alter urinary Mg2+ excretion. Finally, in vitro evaluation of CaSR-driven Cldn14 promoter activity in response to increasing Mg2+ concentrations revealed that Cldn14 expression only increases at supraphysiological extracellular Mg2+ levels. Together, these results suggest that CLDN14 is not involved in regulating extracellular Mg2+ balance following high dietary Mg2+ intake.</description><identifier>ISSN: 1931-857X</identifier><identifier>EISSN: 1522-1466</identifier><identifier>DOI: 10.1152/ajprenal.00590.2020</identifier><language>eng</language><publisher>Bethesda: American Physiological Society</publisher><subject>Calcium (urinary) ; Calcium homeostasis ; Calcium-sensing receptors ; Diet ; Dietary intake ; Excretion ; Homeostasis ; Hypercalcemia ; Hypermagnesemia ; Kidneys ; Magnesium ; Minerals ; Reabsorption ; Tight junctions</subject><ispartof>American journal of physiology. Renal physiology, 2021-05, Vol.320 (5), p.F897-F907</ispartof><rights>Copyright American Physiological Society May 2021</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>Ferreira, Patrícia G</creatorcontrib><creatorcontrib>van Megen, Wouter H</creatorcontrib><creatorcontrib>Tan, Rebecca</creatorcontrib><creatorcontrib>L Lee, Christy H</creatorcontrib><creatorcontrib>Svenningsen, Per</creatorcontrib><creatorcontrib>Alexander, R Todd</creatorcontrib><creatorcontrib>Dimke, Henrik</creatorcontrib><title>Renal claudin-14 expression is not required for regulating Mg2+ balance in mice</title><title>American journal of physiology. Renal physiology</title><description>The kidneys play a crucial role in maintaining Ca2+ and Mg2+ homeostasis by regulating these minerals' reabsorption. In the thick ascending limb of Henle's loop (TAL), Ca2+ and Mg2+ are reabsorbed through the tight junctions by a shared paracellular pathway formed by claudin-16 and claudin-19. Hypercalcemia activates the Ca2+-sensing receptor (CaSR) in the TAL, causing upregulation of pore-blocking claudin-14 (CLDN14), which reduces Ca2+ and Mg2+ reabsorption from this segment. In addition, a high-Mg2+ diet is known to increase both urinary Mg2+ and Ca2+ excretion. Since Mg2+ may also activate CaSR, we aimed to investigate whether CaSR-dependent increases in CLDN14 expression also regulate urinary Mg2+ excretion in response to hypermagnesemia. Here, we show that a Mg2+-enriched diet increased urinary Mg2+ and Ca2+ excretion in mice; however, this occurred without detectable changes in renal CLDN14 expression. The administration of a high-Mg2+ diet to Cldn14−/− mice did not cause more pronounced hypermagnesemia or significantly alter urinary Mg2+ excretion. Finally, in vitro evaluation of CaSR-driven Cldn14 promoter activity in response to increasing Mg2+ concentrations revealed that Cldn14 expression only increases at supraphysiological extracellular Mg2+ levels. Together, these results suggest that CLDN14 is not involved in regulating extracellular Mg2+ balance following high dietary Mg2+ intake.</description><subject>Calcium (urinary)</subject><subject>Calcium homeostasis</subject><subject>Calcium-sensing receptors</subject><subject>Diet</subject><subject>Dietary intake</subject><subject>Excretion</subject><subject>Homeostasis</subject><subject>Hypercalcemia</subject><subject>Hypermagnesemia</subject><subject>Kidneys</subject><subject>Magnesium</subject><subject>Minerals</subject><subject>Reabsorption</subject><subject>Tight junctions</subject><issn>1931-857X</issn><issn>1522-1466</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNpdj09LxDAQxYMouK5-Ai8BL4K0TiZt0hxl8R-sLIiCtyXtTkqWbrrbtODHN4uePM0M83vvzTB2LSAXosR7u90PFGyXA5QGcgSEEzZLG8xEodRp6o0UWVXqr3N2EeMWAIRAMWOr96OON52dNj4kmtN38orR94H7yEM_8oEOkx9ow10_pKGdOjv60PK3Fu94bTsbGuI-8J1v6JKdOdtFuvqrc_b59PixeMmWq-fXxcMy26MSY6YbqmunKietrkAZA5pQ1spIR1pDKaxG1M6BbZCsU4VOnKCilEkJZOSc3f767of-MFEc1zsfG-rSMdRPcY0lVJXBQuuE3vxDt_00pK-PlExZgIjyB_bPX5A</recordid><startdate>20210501</startdate><enddate>20210501</enddate><creator>Ferreira, Patrícia G</creator><creator>van Megen, Wouter H</creator><creator>Tan, Rebecca</creator><creator>L Lee, Christy H</creator><creator>Svenningsen, Per</creator><creator>Alexander, R Todd</creator><creator>Dimke, Henrik</creator><general>American Physiological Society</general><scope>7X8</scope></search><sort><creationdate>20210501</creationdate><title>Renal claudin-14 expression is not required for regulating Mg2+ balance in mice</title><author>Ferreira, Patrícia G ; van Megen, Wouter H ; Tan, Rebecca ; L Lee, Christy H ; Svenningsen, Per ; Alexander, R Todd ; Dimke, Henrik</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p261t-7cebbf68f3a78069907e23b693fe77051a7227ff0ac2eaf647a781e4537ce0e93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Calcium (urinary)</topic><topic>Calcium homeostasis</topic><topic>Calcium-sensing receptors</topic><topic>Diet</topic><topic>Dietary intake</topic><topic>Excretion</topic><topic>Homeostasis</topic><topic>Hypercalcemia</topic><topic>Hypermagnesemia</topic><topic>Kidneys</topic><topic>Magnesium</topic><topic>Minerals</topic><topic>Reabsorption</topic><topic>Tight junctions</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ferreira, Patrícia G</creatorcontrib><creatorcontrib>van Megen, Wouter H</creatorcontrib><creatorcontrib>Tan, Rebecca</creatorcontrib><creatorcontrib>L Lee, Christy H</creatorcontrib><creatorcontrib>Svenningsen, Per</creatorcontrib><creatorcontrib>Alexander, R Todd</creatorcontrib><creatorcontrib>Dimke, Henrik</creatorcontrib><collection>MEDLINE - Academic</collection><jtitle>American journal of physiology. Renal physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ferreira, Patrícia G</au><au>van Megen, Wouter H</au><au>Tan, Rebecca</au><au>L Lee, Christy H</au><au>Svenningsen, Per</au><au>Alexander, R Todd</au><au>Dimke, Henrik</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Renal claudin-14 expression is not required for regulating Mg2+ balance in mice</atitle><jtitle>American journal of physiology. Renal physiology</jtitle><date>2021-05-01</date><risdate>2021</risdate><volume>320</volume><issue>5</issue><spage>F897</spage><epage>F907</epage><pages>F897-F907</pages><issn>1931-857X</issn><eissn>1522-1466</eissn><abstract>The kidneys play a crucial role in maintaining Ca2+ and Mg2+ homeostasis by regulating these minerals' reabsorption. In the thick ascending limb of Henle's loop (TAL), Ca2+ and Mg2+ are reabsorbed through the tight junctions by a shared paracellular pathway formed by claudin-16 and claudin-19. Hypercalcemia activates the Ca2+-sensing receptor (CaSR) in the TAL, causing upregulation of pore-blocking claudin-14 (CLDN14), which reduces Ca2+ and Mg2+ reabsorption from this segment. In addition, a high-Mg2+ diet is known to increase both urinary Mg2+ and Ca2+ excretion. Since Mg2+ may also activate CaSR, we aimed to investigate whether CaSR-dependent increases in CLDN14 expression also regulate urinary Mg2+ excretion in response to hypermagnesemia. Here, we show that a Mg2+-enriched diet increased urinary Mg2+ and Ca2+ excretion in mice; however, this occurred without detectable changes in renal CLDN14 expression. The administration of a high-Mg2+ diet to Cldn14−/− mice did not cause more pronounced hypermagnesemia or significantly alter urinary Mg2+ excretion. Finally, in vitro evaluation of CaSR-driven Cldn14 promoter activity in response to increasing Mg2+ concentrations revealed that Cldn14 expression only increases at supraphysiological extracellular Mg2+ levels. Together, these results suggest that CLDN14 is not involved in regulating extracellular Mg2+ balance following high dietary Mg2+ intake.</abstract><cop>Bethesda</cop><pub>American Physiological Society</pub><doi>10.1152/ajprenal.00590.2020</doi><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 1931-857X
ispartof American journal of physiology. Renal physiology, 2021-05, Vol.320 (5), p.F897-F907
issn 1931-857X
1522-1466
language eng
recordid cdi_proquest_miscellaneous_2508892477
source American Physiological Society; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection
subjects Calcium (urinary)
Calcium homeostasis
Calcium-sensing receptors
Diet
Dietary intake
Excretion
Homeostasis
Hypercalcemia
Hypermagnesemia
Kidneys
Magnesium
Minerals
Reabsorption
Tight junctions
title Renal claudin-14 expression is not required for regulating Mg2+ balance in mice
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-24T03%3A40%3A47IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Renal%20claudin-14%20expression%20is%20not%20required%20for%20regulating%20Mg2+%20balance%20in%20mice&rft.jtitle=American%20journal%20of%20physiology.%20Renal%20physiology&rft.au=Ferreira,%20Patr%C3%ADcia%20G&rft.date=2021-05-01&rft.volume=320&rft.issue=5&rft.spage=F897&rft.epage=F907&rft.pages=F897-F907&rft.issn=1931-857X&rft.eissn=1522-1466&rft_id=info:doi/10.1152/ajprenal.00590.2020&rft_dat=%3Cproquest%3E2508892477%3C/proquest%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2530510222&rft_id=info:pmid/&rfr_iscdi=true