Impact of storage temperature, storage duration, and deproteinization on plasma amino acid concentrations in dogs
Reliability of canine plasma amino acid analysis depends on sample stability which can be influenced by pre-analytical handling techniques, storage temperature, storage time, and deproteinization status. Extrapolating data to dogs from research in other species is limited given discordant methodolog...
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| Veröffentlicht in: | Research in veterinary science 2021-05, Vol.136, p.416-421 |
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| creator | Chiang, Chih-Fan Larsen, Jennifer A. Sahtout, Mohammad Horoschak, Rachael E. Yu, Zeng-Shou Fascetti, Andrea J. |
| description | Reliability of canine plasma amino acid analysis depends on sample stability which can be influenced by pre-analytical handling techniques, storage temperature, storage time, and deproteinization status. Extrapolating data to dogs from research in other species is limited given discordant methodology and interspecies differences. The present study investigated the effects of deproteinization status (non-deproteinized or deproteinized) and storage temperature (at −20 °C or − 80 °C) on the concentration of 22 canine plasma amino acids during a 300-day storage period.
Storage time had a significant effect (p |
| doi_str_mv | 10.1016/j.rvsc.2021.03.022 |
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Storage time had a significant effect (p < 0.05) of overall declining concentration of most amino acids. Compared to non-deproteinized samples, deproteinization contributed to overall higher concentrations of cyst(e)ine and glutamic acid, and consistently modified the effect of storage time and temperature on cyst(e)ine, glutamic acid, and glutamine. Compared to −20 °C, storage at −80 °C contributed to a higher concentration of cyst(e)ine and glutamic acid, and modified the effect of storage time on arginine, glutamic acid, glutamine, and tryptophan.
Storage time had a consistent, significant effect on amino acid concentrations in canine plasma samples. Although sample deproteinization and low storage temperature modified the effect of storage time, these interactions were variable among analyzed amino acids. Therefore, timely sample analysis is recommended. If delayed sample analysis is inevitable, deproteinization should be performed prior to sample banking to preserve amino acid stability.
•The concentration of canine plasma amino acids fluctuated during 300-day storage.•Storage time had a significant effect on the concentration of all amino acids in canine plasma.•Timely analysis, deproteinization, and low storage temperature are recommended.</description><identifier>ISSN: 0034-5288</identifier><identifier>EISSN: 1532-2661</identifier><identifier>DOI: 10.1016/j.rvsc.2021.03.022</identifier><identifier>PMID: 33812284</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Amino acids ; Automation ; Canine ; Cysts ; Deproteinization ; Dogs ; Glutamic acid ; Glutamine ; Laboratories ; Plasma ; Plasma amino acid concentration ; Pre-analytical handling ; Reliability analysis ; Stability analysis ; Storage temperature ; Storage time ; Temperature ; Tryptophan ; Veterinary medicine</subject><ispartof>Research in veterinary science, 2021-05, Vol.136, p.416-421</ispartof><rights>2021 Elsevier Ltd</rights><rights>Copyright © 2021 Elsevier Ltd. All rights reserved.</rights><rights>2021. Elsevier Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c384t-1a01c3a0d977aa92332ccbbbdb45e416bb2578bcf4b7932c87a15950f70cc38a3</citedby><cites>FETCH-LOGICAL-c384t-1a01c3a0d977aa92332ccbbbdb45e416bb2578bcf4b7932c87a15950f70cc38a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S003452882100093X$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,65309</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33812284$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chiang, Chih-Fan</creatorcontrib><creatorcontrib>Larsen, Jennifer A.</creatorcontrib><creatorcontrib>Sahtout, Mohammad</creatorcontrib><creatorcontrib>Horoschak, Rachael E.</creatorcontrib><creatorcontrib>Yu, Zeng-Shou</creatorcontrib><creatorcontrib>Fascetti, Andrea J.</creatorcontrib><title>Impact of storage temperature, storage duration, and deproteinization on plasma amino acid concentrations in dogs</title><title>Research in veterinary science</title><addtitle>Res Vet Sci</addtitle><description>Reliability of canine plasma amino acid analysis depends on sample stability which can be influenced by pre-analytical handling techniques, storage temperature, storage time, and deproteinization status. Extrapolating data to dogs from research in other species is limited given discordant methodology and interspecies differences. The present study investigated the effects of deproteinization status (non-deproteinized or deproteinized) and storage temperature (at −20 °C or − 80 °C) on the concentration of 22 canine plasma amino acids during a 300-day storage period.
Storage time had a significant effect (p < 0.05) of overall declining concentration of most amino acids. Compared to non-deproteinized samples, deproteinization contributed to overall higher concentrations of cyst(e)ine and glutamic acid, and consistently modified the effect of storage time and temperature on cyst(e)ine, glutamic acid, and glutamine. Compared to −20 °C, storage at −80 °C contributed to a higher concentration of cyst(e)ine and glutamic acid, and modified the effect of storage time on arginine, glutamic acid, glutamine, and tryptophan.
Storage time had a consistent, significant effect on amino acid concentrations in canine plasma samples. Although sample deproteinization and low storage temperature modified the effect of storage time, these interactions were variable among analyzed amino acids. Therefore, timely sample analysis is recommended. If delayed sample analysis is inevitable, deproteinization should be performed prior to sample banking to preserve amino acid stability.
•The concentration of canine plasma amino acids fluctuated during 300-day storage.•Storage time had a significant effect on the concentration of all amino acids in canine plasma.•Timely analysis, deproteinization, and low storage temperature are recommended.</description><subject>Amino acids</subject><subject>Automation</subject><subject>Canine</subject><subject>Cysts</subject><subject>Deproteinization</subject><subject>Dogs</subject><subject>Glutamic acid</subject><subject>Glutamine</subject><subject>Laboratories</subject><subject>Plasma</subject><subject>Plasma amino acid concentration</subject><subject>Pre-analytical handling</subject><subject>Reliability analysis</subject><subject>Stability analysis</subject><subject>Storage temperature</subject><subject>Storage time</subject><subject>Temperature</subject><subject>Tryptophan</subject><subject>Veterinary medicine</subject><issn>0034-5288</issn><issn>1532-2661</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNp9kc1rFTEUxYMo9rX6D7iQgBsXnTEfk0kG3Ejxo1Bwo-uQjzsljzfJNMkU7F9vnq924UIIBM79ncPlHoTeUNJTQscP-z7fF9czwmhPeE8Ye4Z2VHDWsXGkz9GOED50gil1hs5L2RNCBkrlS3TGuaKMqWGH7q6X1biK04xLTdncAq6wrJBN3TJcPol-a0pI8RKb6LGHNacKIYaHPypubz2YshhslhATNi547FJ0EOvJWHCI2Kfb8gq9mM2hwOvH_wL9_PL5x9W37ub71-urTzed42qoHTWEOm6In6Q0ZmKcM-estd4OAgY6WsuEVNbNg5VTmylpqJgEmSVxLcHwC_T-lNtWvdugVL2E4uBwMBHSVjQTRIlxGgfV0Hf_oPu05di2axSTggsqWaPYiXI5lZJh1msOi8m_NCX6WIje62Mh-liIJly3Qprp7WP0ZhfwT5a_DTTg4wmAdov7AFkXF6AdzocMrmqfwv_yfwPTPp2b</recordid><startdate>202105</startdate><enddate>202105</enddate><creator>Chiang, Chih-Fan</creator><creator>Larsen, Jennifer A.</creator><creator>Sahtout, Mohammad</creator><creator>Horoschak, Rachael E.</creator><creator>Yu, Zeng-Shou</creator><creator>Fascetti, Andrea J.</creator><general>Elsevier Ltd</general><general>Elsevier Limited</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QP</scope><scope>7QR</scope><scope>7T5</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>202105</creationdate><title>Impact of storage temperature, storage duration, and deproteinization on plasma amino acid concentrations in dogs</title><author>Chiang, Chih-Fan ; Larsen, Jennifer A. ; Sahtout, Mohammad ; Horoschak, Rachael E. ; Yu, Zeng-Shou ; Fascetti, Andrea J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c384t-1a01c3a0d977aa92332ccbbbdb45e416bb2578bcf4b7932c87a15950f70cc38a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Amino acids</topic><topic>Automation</topic><topic>Canine</topic><topic>Cysts</topic><topic>Deproteinization</topic><topic>Dogs</topic><topic>Glutamic acid</topic><topic>Glutamine</topic><topic>Laboratories</topic><topic>Plasma</topic><topic>Plasma amino acid concentration</topic><topic>Pre-analytical handling</topic><topic>Reliability analysis</topic><topic>Stability analysis</topic><topic>Storage temperature</topic><topic>Storage time</topic><topic>Temperature</topic><topic>Tryptophan</topic><topic>Veterinary medicine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chiang, Chih-Fan</creatorcontrib><creatorcontrib>Larsen, Jennifer A.</creatorcontrib><creatorcontrib>Sahtout, Mohammad</creatorcontrib><creatorcontrib>Horoschak, Rachael E.</creatorcontrib><creatorcontrib>Yu, Zeng-Shou</creatorcontrib><creatorcontrib>Fascetti, Andrea J.</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Animal Behavior Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Research in veterinary science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chiang, Chih-Fan</au><au>Larsen, Jennifer A.</au><au>Sahtout, Mohammad</au><au>Horoschak, Rachael E.</au><au>Yu, Zeng-Shou</au><au>Fascetti, Andrea J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Impact of storage temperature, storage duration, and deproteinization on plasma amino acid concentrations in dogs</atitle><jtitle>Research in veterinary science</jtitle><addtitle>Res Vet Sci</addtitle><date>2021-05</date><risdate>2021</risdate><volume>136</volume><spage>416</spage><epage>421</epage><pages>416-421</pages><issn>0034-5288</issn><eissn>1532-2661</eissn><abstract>Reliability of canine plasma amino acid analysis depends on sample stability which can be influenced by pre-analytical handling techniques, storage temperature, storage time, and deproteinization status. Extrapolating data to dogs from research in other species is limited given discordant methodology and interspecies differences. The present study investigated the effects of deproteinization status (non-deproteinized or deproteinized) and storage temperature (at −20 °C or − 80 °C) on the concentration of 22 canine plasma amino acids during a 300-day storage period.
Storage time had a significant effect (p < 0.05) of overall declining concentration of most amino acids. Compared to non-deproteinized samples, deproteinization contributed to overall higher concentrations of cyst(e)ine and glutamic acid, and consistently modified the effect of storage time and temperature on cyst(e)ine, glutamic acid, and glutamine. Compared to −20 °C, storage at −80 °C contributed to a higher concentration of cyst(e)ine and glutamic acid, and modified the effect of storage time on arginine, glutamic acid, glutamine, and tryptophan.
Storage time had a consistent, significant effect on amino acid concentrations in canine plasma samples. Although sample deproteinization and low storage temperature modified the effect of storage time, these interactions were variable among analyzed amino acids. Therefore, timely sample analysis is recommended. If delayed sample analysis is inevitable, deproteinization should be performed prior to sample banking to preserve amino acid stability.
•The concentration of canine plasma amino acids fluctuated during 300-day storage.•Storage time had a significant effect on the concentration of all amino acids in canine plasma.•Timely analysis, deproteinization, and low storage temperature are recommended.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>33812284</pmid><doi>10.1016/j.rvsc.2021.03.022</doi><tpages>6</tpages></addata></record> |
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| subjects | Amino acids Automation Canine Cysts Deproteinization Dogs Glutamic acid Glutamine Laboratories Plasma Plasma amino acid concentration Pre-analytical handling Reliability analysis Stability analysis Storage temperature Storage time Temperature Tryptophan Veterinary medicine |
| title | Impact of storage temperature, storage duration, and deproteinization on plasma amino acid concentrations in dogs |
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