Long noncoding RNA SNHG9 facilitates growth of glioma stem‐like cells via miR‐326/SOX9 axis
Background Glioma stem‐like cells (GSCs) are greatly responsible for the progression of glioma. Long noncoding RNAs (lncRNAs) play an important role in glioma tumor progression. This study aims to explore the role and underlying mechanism of lncRNA SNHG9 in regulating GSC cell growth. Methods GSCs w...
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| Veröffentlicht in: | The journal of gene medicine 2022-01, Vol.24 (1), p.e3334-n/a |
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| creator | Wang, Chao‐Jie Chao, Chu‐Rui Zhao, Wei‐Feng Liu, Hui‐Min Feng, Jiang‐Shan Cui, Yong‐Xia |
| description | Background
Glioma stem‐like cells (GSCs) are greatly responsible for the progression of glioma. Long noncoding RNAs (lncRNAs) play an important role in glioma tumor progression. This study aims to explore the role and underlying mechanism of lncRNA SNHG9 in regulating GSC cell growth.
Methods
GSCs were obtained from glioma cells (U87 and U251) and referred to as GSC‐87 and GSC‐251, respectively. The interactions between miR‐326 and SNHG9 or SOX9 were analyzed using luciferase reporter assay. Cell growth of GSCs was evaluated by EdU assay and sphere formation assay.
Results
SNHG9 expression was significantly higher in GSC‐87 and GSC‐251 cells than in U87 and U251 cells. SNHG9 overexpression promoted GSC cell growth, whereas SNHG9 knockdown inhibited GSC cell growth. Mechanistically, SNHG9 acted as a competitive endogenous RNA of miR‐326 to elevate the expression of SOX9, a direct target of miR‐326. Moreover, transfection with miR‐326 inhibitor counteracted SNHG9 knockdown‐mediated inhibition of GSC cell growth.
Conclusions
SNHG9 facilitates growth of GSCs via the miR‐326/SOX9 axis. This study provides a promising therapeutic target for glioma treatment.
LncRNA SNHG9 functions as a ceRNA of miR‐326 to elevate expression of SOX9, and thereby promotes cell proliferation of glioma stem cells (GSCs). Our findings suggest that SNHG9 may be a promising therapeutic target for the treatment of glioma. |
| doi_str_mv | 10.1002/jgm.3334 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2507730334</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2615878287</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3494-a738a96b68d9bb0e7f945a08eb7efbb70becb95395228856b48f9eeb4b5331ae3</originalsourceid><addsrcrecordid>eNp1kM1KAzEUhYMo_lTBJ5CAGzfTZpLJJFmKaFWqharQXUimd2rqzEQnU6s7H8Fn9EkcrT8guLqHy8fH4SC0G5NuTAjtzaZllzGWrKDNmNM4opQnq20mSkWJkuMNtBXCjJBYSKnW0QZjQirG1SbSA19NceWrzE9cm0aXh_jq8rSvcG4yV7jGNBDwtPaL5hb7HE8L50uDQwPl28tr4e4AZ1AUAT86g0s3ap-Mpr2r4Vhh8-TCNlrLTRFg5-t20M3J8fXRaTQY9s-ODgdRxhKVREYwaVRqUzlR1hIQuUq4IRKsgNxaQSxkVnGmOKVS8tQmMlcANrGcsdgA66CDpfe-9g9zCI0uXfhoZirw86ApJ0Iw0m7Uovt_0Jmf11XbTtM05lJIKsWvMKt9CDXk-r52pamfdUz0x-i6HV2zpXDvSzi3JUx-wO-VWyBaAgtXwPO_In3ev_gUvgM6Yoq9</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2615878287</pqid></control><display><type>article</type><title>Long noncoding RNA SNHG9 facilitates growth of glioma stem‐like cells via miR‐326/SOX9 axis</title><source>MEDLINE</source><source>Wiley Journals</source><creator>Wang, Chao‐Jie ; Chao, Chu‐Rui ; Zhao, Wei‐Feng ; Liu, Hui‐Min ; Feng, Jiang‐Shan ; Cui, Yong‐Xia</creator><creatorcontrib>Wang, Chao‐Jie ; Chao, Chu‐Rui ; Zhao, Wei‐Feng ; Liu, Hui‐Min ; Feng, Jiang‐Shan ; Cui, Yong‐Xia</creatorcontrib><description>Background
Glioma stem‐like cells (GSCs) are greatly responsible for the progression of glioma. Long noncoding RNAs (lncRNAs) play an important role in glioma tumor progression. This study aims to explore the role and underlying mechanism of lncRNA SNHG9 in regulating GSC cell growth.
Methods
GSCs were obtained from glioma cells (U87 and U251) and referred to as GSC‐87 and GSC‐251, respectively. The interactions between miR‐326 and SNHG9 or SOX9 were analyzed using luciferase reporter assay. Cell growth of GSCs was evaluated by EdU assay and sphere formation assay.
Results
SNHG9 expression was significantly higher in GSC‐87 and GSC‐251 cells than in U87 and U251 cells. SNHG9 overexpression promoted GSC cell growth, whereas SNHG9 knockdown inhibited GSC cell growth. Mechanistically, SNHG9 acted as a competitive endogenous RNA of miR‐326 to elevate the expression of SOX9, a direct target of miR‐326. Moreover, transfection with miR‐326 inhibitor counteracted SNHG9 knockdown‐mediated inhibition of GSC cell growth.
Conclusions
SNHG9 facilitates growth of GSCs via the miR‐326/SOX9 axis. This study provides a promising therapeutic target for glioma treatment.
LncRNA SNHG9 functions as a ceRNA of miR‐326 to elevate expression of SOX9, and thereby promotes cell proliferation of glioma stem cells (GSCs). Our findings suggest that SNHG9 may be a promising therapeutic target for the treatment of glioma.</description><identifier>ISSN: 1099-498X</identifier><identifier>EISSN: 1521-2254</identifier><identifier>DOI: 10.1002/jgm.3334</identifier><identifier>PMID: 33789359</identifier><language>eng</language><publisher>England: Wiley Periodicals Inc</publisher><subject>Brain Neoplasms - genetics ; Brain Neoplasms - pathology ; Brain tumors ; Cell growth ; Cell Line, Tumor ; Cell Proliferation - genetics ; Gene Expression Regulation, Neoplastic ; Gene therapy ; Glioma ; Glioma - pathology ; Glioma cells ; glioma stem cell ; Humans ; MicroRNAs - genetics ; MicroRNAs - metabolism ; miR‐326 ; Neoplastic Stem Cells - metabolism ; Neoplastic Stem Cells - pathology ; Non-coding RNA ; RNA, Long Noncoding - genetics ; RNA, Long Noncoding - metabolism ; SNHG9 ; SOX9 ; Sox9 protein ; SOX9 Transcription Factor - genetics ; SOX9 Transcription Factor - metabolism ; Therapeutic targets ; Transfection</subject><ispartof>The journal of gene medicine, 2022-01, Vol.24 (1), p.e3334-n/a</ispartof><rights>2021 John Wiley & Sons, Ltd.</rights><rights>2022 John Wiley & Sons, Ltd.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3494-a738a96b68d9bb0e7f945a08eb7efbb70becb95395228856b48f9eeb4b5331ae3</citedby><cites>FETCH-LOGICAL-c3494-a738a96b68d9bb0e7f945a08eb7efbb70becb95395228856b48f9eeb4b5331ae3</cites><orcidid>0000-0001-7887-315X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjgm.3334$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjgm.3334$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33789359$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Chao‐Jie</creatorcontrib><creatorcontrib>Chao, Chu‐Rui</creatorcontrib><creatorcontrib>Zhao, Wei‐Feng</creatorcontrib><creatorcontrib>Liu, Hui‐Min</creatorcontrib><creatorcontrib>Feng, Jiang‐Shan</creatorcontrib><creatorcontrib>Cui, Yong‐Xia</creatorcontrib><title>Long noncoding RNA SNHG9 facilitates growth of glioma stem‐like cells via miR‐326/SOX9 axis</title><title>The journal of gene medicine</title><addtitle>J Gene Med</addtitle><description>Background
Glioma stem‐like cells (GSCs) are greatly responsible for the progression of glioma. Long noncoding RNAs (lncRNAs) play an important role in glioma tumor progression. This study aims to explore the role and underlying mechanism of lncRNA SNHG9 in regulating GSC cell growth.
Methods
GSCs were obtained from glioma cells (U87 and U251) and referred to as GSC‐87 and GSC‐251, respectively. The interactions between miR‐326 and SNHG9 or SOX9 were analyzed using luciferase reporter assay. Cell growth of GSCs was evaluated by EdU assay and sphere formation assay.
Results
SNHG9 expression was significantly higher in GSC‐87 and GSC‐251 cells than in U87 and U251 cells. SNHG9 overexpression promoted GSC cell growth, whereas SNHG9 knockdown inhibited GSC cell growth. Mechanistically, SNHG9 acted as a competitive endogenous RNA of miR‐326 to elevate the expression of SOX9, a direct target of miR‐326. Moreover, transfection with miR‐326 inhibitor counteracted SNHG9 knockdown‐mediated inhibition of GSC cell growth.
Conclusions
SNHG9 facilitates growth of GSCs via the miR‐326/SOX9 axis. This study provides a promising therapeutic target for glioma treatment.
LncRNA SNHG9 functions as a ceRNA of miR‐326 to elevate expression of SOX9, and thereby promotes cell proliferation of glioma stem cells (GSCs). Our findings suggest that SNHG9 may be a promising therapeutic target for the treatment of glioma.</description><subject>Brain Neoplasms - genetics</subject><subject>Brain Neoplasms - pathology</subject><subject>Brain tumors</subject><subject>Cell growth</subject><subject>Cell Line, Tumor</subject><subject>Cell Proliferation - genetics</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>Gene therapy</subject><subject>Glioma</subject><subject>Glioma - pathology</subject><subject>Glioma cells</subject><subject>glioma stem cell</subject><subject>Humans</subject><subject>MicroRNAs - genetics</subject><subject>MicroRNAs - metabolism</subject><subject>miR‐326</subject><subject>Neoplastic Stem Cells - metabolism</subject><subject>Neoplastic Stem Cells - pathology</subject><subject>Non-coding RNA</subject><subject>RNA, Long Noncoding - genetics</subject><subject>RNA, Long Noncoding - metabolism</subject><subject>SNHG9</subject><subject>SOX9</subject><subject>Sox9 protein</subject><subject>SOX9 Transcription Factor - genetics</subject><subject>SOX9 Transcription Factor - metabolism</subject><subject>Therapeutic targets</subject><subject>Transfection</subject><issn>1099-498X</issn><issn>1521-2254</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kM1KAzEUhYMo_lTBJ5CAGzfTZpLJJFmKaFWqharQXUimd2rqzEQnU6s7H8Fn9EkcrT8guLqHy8fH4SC0G5NuTAjtzaZllzGWrKDNmNM4opQnq20mSkWJkuMNtBXCjJBYSKnW0QZjQirG1SbSA19NceWrzE9cm0aXh_jq8rSvcG4yV7jGNBDwtPaL5hb7HE8L50uDQwPl28tr4e4AZ1AUAT86g0s3ap-Mpr2r4Vhh8-TCNlrLTRFg5-t20M3J8fXRaTQY9s-ODgdRxhKVREYwaVRqUzlR1hIQuUq4IRKsgNxaQSxkVnGmOKVS8tQmMlcANrGcsdgA66CDpfe-9g9zCI0uXfhoZirw86ApJ0Iw0m7Uovt_0Jmf11XbTtM05lJIKsWvMKt9CDXk-r52pamfdUz0x-i6HV2zpXDvSzi3JUx-wO-VWyBaAgtXwPO_In3ev_gUvgM6Yoq9</recordid><startdate>202201</startdate><enddate>202201</enddate><creator>Wang, Chao‐Jie</creator><creator>Chao, Chu‐Rui</creator><creator>Zhao, Wei‐Feng</creator><creator>Liu, Hui‐Min</creator><creator>Feng, Jiang‐Shan</creator><creator>Cui, Yong‐Xia</creator><general>Wiley Periodicals Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7TK</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>K9.</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-7887-315X</orcidid></search><sort><creationdate>202201</creationdate><title>Long noncoding RNA SNHG9 facilitates growth of glioma stem‐like cells via miR‐326/SOX9 axis</title><author>Wang, Chao‐Jie ; Chao, Chu‐Rui ; Zhao, Wei‐Feng ; Liu, Hui‐Min ; Feng, Jiang‐Shan ; Cui, Yong‐Xia</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3494-a738a96b68d9bb0e7f945a08eb7efbb70becb95395228856b48f9eeb4b5331ae3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Brain Neoplasms - genetics</topic><topic>Brain Neoplasms - pathology</topic><topic>Brain tumors</topic><topic>Cell growth</topic><topic>Cell Line, Tumor</topic><topic>Cell Proliferation - genetics</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>Gene therapy</topic><topic>Glioma</topic><topic>Glioma - pathology</topic><topic>Glioma cells</topic><topic>glioma stem cell</topic><topic>Humans</topic><topic>MicroRNAs - genetics</topic><topic>MicroRNAs - metabolism</topic><topic>miR‐326</topic><topic>Neoplastic Stem Cells - metabolism</topic><topic>Neoplastic Stem Cells - pathology</topic><topic>Non-coding RNA</topic><topic>RNA, Long Noncoding - genetics</topic><topic>RNA, Long Noncoding - metabolism</topic><topic>SNHG9</topic><topic>SOX9</topic><topic>Sox9 protein</topic><topic>SOX9 Transcription Factor - genetics</topic><topic>SOX9 Transcription Factor - metabolism</topic><topic>Therapeutic targets</topic><topic>Transfection</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Chao‐Jie</creatorcontrib><creatorcontrib>Chao, Chu‐Rui</creatorcontrib><creatorcontrib>Zhao, Wei‐Feng</creatorcontrib><creatorcontrib>Liu, Hui‐Min</creatorcontrib><creatorcontrib>Feng, Jiang‐Shan</creatorcontrib><creatorcontrib>Cui, Yong‐Xia</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>The journal of gene medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Chao‐Jie</au><au>Chao, Chu‐Rui</au><au>Zhao, Wei‐Feng</au><au>Liu, Hui‐Min</au><au>Feng, Jiang‐Shan</au><au>Cui, Yong‐Xia</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Long noncoding RNA SNHG9 facilitates growth of glioma stem‐like cells via miR‐326/SOX9 axis</atitle><jtitle>The journal of gene medicine</jtitle><addtitle>J Gene Med</addtitle><date>2022-01</date><risdate>2022</risdate><volume>24</volume><issue>1</issue><spage>e3334</spage><epage>n/a</epage><pages>e3334-n/a</pages><issn>1099-498X</issn><eissn>1521-2254</eissn><abstract>Background
Glioma stem‐like cells (GSCs) are greatly responsible for the progression of glioma. Long noncoding RNAs (lncRNAs) play an important role in glioma tumor progression. This study aims to explore the role and underlying mechanism of lncRNA SNHG9 in regulating GSC cell growth.
Methods
GSCs were obtained from glioma cells (U87 and U251) and referred to as GSC‐87 and GSC‐251, respectively. The interactions between miR‐326 and SNHG9 or SOX9 were analyzed using luciferase reporter assay. Cell growth of GSCs was evaluated by EdU assay and sphere formation assay.
Results
SNHG9 expression was significantly higher in GSC‐87 and GSC‐251 cells than in U87 and U251 cells. SNHG9 overexpression promoted GSC cell growth, whereas SNHG9 knockdown inhibited GSC cell growth. Mechanistically, SNHG9 acted as a competitive endogenous RNA of miR‐326 to elevate the expression of SOX9, a direct target of miR‐326. Moreover, transfection with miR‐326 inhibitor counteracted SNHG9 knockdown‐mediated inhibition of GSC cell growth.
Conclusions
SNHG9 facilitates growth of GSCs via the miR‐326/SOX9 axis. This study provides a promising therapeutic target for glioma treatment.
LncRNA SNHG9 functions as a ceRNA of miR‐326 to elevate expression of SOX9, and thereby promotes cell proliferation of glioma stem cells (GSCs). Our findings suggest that SNHG9 may be a promising therapeutic target for the treatment of glioma.</abstract><cop>England</cop><pub>Wiley Periodicals Inc</pub><pmid>33789359</pmid><doi>10.1002/jgm.3334</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0001-7887-315X</orcidid></addata></record> |
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| subjects | Brain Neoplasms - genetics Brain Neoplasms - pathology Brain tumors Cell growth Cell Line, Tumor Cell Proliferation - genetics Gene Expression Regulation, Neoplastic Gene therapy Glioma Glioma - pathology Glioma cells glioma stem cell Humans MicroRNAs - genetics MicroRNAs - metabolism miR‐326 Neoplastic Stem Cells - metabolism Neoplastic Stem Cells - pathology Non-coding RNA RNA, Long Noncoding - genetics RNA, Long Noncoding - metabolism SNHG9 SOX9 Sox9 protein SOX9 Transcription Factor - genetics SOX9 Transcription Factor - metabolism Therapeutic targets Transfection |
| title | Long noncoding RNA SNHG9 facilitates growth of glioma stem‐like cells via miR‐326/SOX9 axis |
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