The association between initial adhesion and cyanobacterial biofilm development
ABSTRACT Although laboratory assays provide valuable information about the antifouling effectiveness of marine surfaces and the dynamics of biofilm formation, they may be laborious and time-consuming. This study aimed to determine the potential of short-time adhesion assays to estimate how biofilm d...
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| Veröffentlicht in: | FEMS microbiology ecology 2021-04, Vol.97 (5) |
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| creator | Faria, Sara I Teixeira-Santos, Rita Morais, João Vasconcelos, Vitor Mergulhão, Filipe J |
| description | ABSTRACT
Although laboratory assays provide valuable information about the antifouling effectiveness of marine surfaces and the dynamics of biofilm formation, they may be laborious and time-consuming. This study aimed to determine the potential of short-time adhesion assays to estimate how biofilm development may proceed. The initial adhesion and cyanobacterial biofilm formation were evaluated using glass and polymer epoxy resin surfaces under different hydrodynamic conditions and were compared using linear regression models. For initial adhesion, the polymer epoxy resin surface was significantly associated with a lower number of adhered cells compared with glass (-1.27 × 105 cells.cm–2). Likewise, the number of adhered cells was significantly lower (-1.16 × 105 cells.cm–2) at 185 than at 40 rpm. This tendency was maintained during biofilm development and was supported by the biofilm wet weight, thickness, chlorophyll a content and structure. Results indicated a significant correlation between the number of adhered and biofilm cells (r = 0.800, p < 0.001). Moreover, the number of biofilm cells on day 42 was dependent on the number of adhered cells at the end of the initial adhesion and hydrodynamic conditions (R2 = 0.795, p < 0.001). These findings demonstrate the high potential of initial adhesion assays to estimate marine biofilm development.
Initial cell adhesion assays revealed a high potential to estimate cyanobacterial biofilm development, allowing high throughput screening of marine coatings under different conditions and providing indications about the biofilm behaviour in a short time. |
| doi_str_mv | 10.1093/femsec/fiab052 |
| format | Article |
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Although laboratory assays provide valuable information about the antifouling effectiveness of marine surfaces and the dynamics of biofilm formation, they may be laborious and time-consuming. This study aimed to determine the potential of short-time adhesion assays to estimate how biofilm development may proceed. The initial adhesion and cyanobacterial biofilm formation were evaluated using glass and polymer epoxy resin surfaces under different hydrodynamic conditions and were compared using linear regression models. For initial adhesion, the polymer epoxy resin surface was significantly associated with a lower number of adhered cells compared with glass (-1.27 × 105 cells.cm–2). Likewise, the number of adhered cells was significantly lower (-1.16 × 105 cells.cm–2) at 185 than at 40 rpm. This tendency was maintained during biofilm development and was supported by the biofilm wet weight, thickness, chlorophyll a content and structure. Results indicated a significant correlation between the number of adhered and biofilm cells (r = 0.800, p < 0.001). Moreover, the number of biofilm cells on day 42 was dependent on the number of adhered cells at the end of the initial adhesion and hydrodynamic conditions (R2 = 0.795, p < 0.001). These findings demonstrate the high potential of initial adhesion assays to estimate marine biofilm development.
Initial cell adhesion assays revealed a high potential to estimate cyanobacterial biofilm development, allowing high throughput screening of marine coatings under different conditions and providing indications about the biofilm behaviour in a short time.</description><identifier>ISSN: 0168-6496</identifier><identifier>EISSN: 1574-6941</identifier><identifier>DOI: 10.1093/femsec/fiab052</identifier><identifier>PMID: 33784393</identifier><language>eng</language><publisher>England: Oxford University Press</publisher><subject>Adhesion ; Antifouling substances ; Assaying ; Bacterial Adhesion ; Biofilms ; Chlorophyll ; Chlorophyll A ; Cyanobacteria ; Ecology ; Epoxy resins ; Microbiology ; Polymers ; Regression analysis ; Regression models</subject><ispartof>FEMS microbiology ecology, 2021-04, Vol.97 (5)</ispartof><rights>The Author(s) 2021. Published by Oxford University Press on behalf of FEMS. 2021</rights><rights>The Author(s) 2021. Published by Oxford University Press on behalf of FEMS.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c357t-511a707bd0d092f01f7b3fdb4f08bd0109a7cb1f30e0881073e184b5f43f36223</citedby><cites>FETCH-LOGICAL-c357t-511a707bd0d092f01f7b3fdb4f08bd0109a7cb1f30e0881073e184b5f43f36223</cites><orcidid>0000-0002-4819-1892 ; 0000-0001-7546-9362</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,1598,27901,27902</link.rule.ids><linktorsrc>$$Uhttps://dx.doi.org/10.1093/femsec/fiab052$$EView_record_in_Oxford_University_Press$$FView_record_in_$$GOxford_University_Press</linktorsrc><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33784393$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Faria, Sara I</creatorcontrib><creatorcontrib>Teixeira-Santos, Rita</creatorcontrib><creatorcontrib>Morais, João</creatorcontrib><creatorcontrib>Vasconcelos, Vitor</creatorcontrib><creatorcontrib>Mergulhão, Filipe J</creatorcontrib><title>The association between initial adhesion and cyanobacterial biofilm development</title><title>FEMS microbiology ecology</title><addtitle>FEMS Microbiol Ecol</addtitle><description>ABSTRACT
Although laboratory assays provide valuable information about the antifouling effectiveness of marine surfaces and the dynamics of biofilm formation, they may be laborious and time-consuming. This study aimed to determine the potential of short-time adhesion assays to estimate how biofilm development may proceed. The initial adhesion and cyanobacterial biofilm formation were evaluated using glass and polymer epoxy resin surfaces under different hydrodynamic conditions and were compared using linear regression models. For initial adhesion, the polymer epoxy resin surface was significantly associated with a lower number of adhered cells compared with glass (-1.27 × 105 cells.cm–2). Likewise, the number of adhered cells was significantly lower (-1.16 × 105 cells.cm–2) at 185 than at 40 rpm. This tendency was maintained during biofilm development and was supported by the biofilm wet weight, thickness, chlorophyll a content and structure. Results indicated a significant correlation between the number of adhered and biofilm cells (r = 0.800, p < 0.001). Moreover, the number of biofilm cells on day 42 was dependent on the number of adhered cells at the end of the initial adhesion and hydrodynamic conditions (R2 = 0.795, p < 0.001). These findings demonstrate the high potential of initial adhesion assays to estimate marine biofilm development.
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Although laboratory assays provide valuable information about the antifouling effectiveness of marine surfaces and the dynamics of biofilm formation, they may be laborious and time-consuming. This study aimed to determine the potential of short-time adhesion assays to estimate how biofilm development may proceed. The initial adhesion and cyanobacterial biofilm formation were evaluated using glass and polymer epoxy resin surfaces under different hydrodynamic conditions and were compared using linear regression models. For initial adhesion, the polymer epoxy resin surface was significantly associated with a lower number of adhered cells compared with glass (-1.27 × 105 cells.cm–2). Likewise, the number of adhered cells was significantly lower (-1.16 × 105 cells.cm–2) at 185 than at 40 rpm. This tendency was maintained during biofilm development and was supported by the biofilm wet weight, thickness, chlorophyll a content and structure. Results indicated a significant correlation between the number of adhered and biofilm cells (r = 0.800, p < 0.001). Moreover, the number of biofilm cells on day 42 was dependent on the number of adhered cells at the end of the initial adhesion and hydrodynamic conditions (R2 = 0.795, p < 0.001). These findings demonstrate the high potential of initial adhesion assays to estimate marine biofilm development.
Initial cell adhesion assays revealed a high potential to estimate cyanobacterial biofilm development, allowing high throughput screening of marine coatings under different conditions and providing indications about the biofilm behaviour in a short time.</abstract><cop>England</cop><pub>Oxford University Press</pub><pmid>33784393</pmid><doi>10.1093/femsec/fiab052</doi><orcidid>https://orcid.org/0000-0002-4819-1892</orcidid><orcidid>https://orcid.org/0000-0001-7546-9362</orcidid></addata></record> |
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| issn | 0168-6496 1574-6941 |
| language | eng |
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| source | Oxford Journals Open Access Collection |
| subjects | Adhesion Antifouling substances Assaying Bacterial Adhesion Biofilms Chlorophyll Chlorophyll A Cyanobacteria Ecology Epoxy resins Microbiology Polymers Regression analysis Regression models |
| title | The association between initial adhesion and cyanobacterial biofilm development |
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