Metal-Free Polymer-Based Affinity Medium for Selective Purification of His6-Tagged Proteins

We report a metal free synthetic hydrogel copolymer with affinity and selectivity for His6-tagged peptides and proteins. Small libraries of copolymers incorporating charged and hydrophobic functional groups were screened by an iterative process for His6 peptide affinity. The monomer selection was gu...

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Veröffentlicht in:	Biomacromolecules 2021-04, Vol.22 (4), p.1695-1705
Hauptverfasser:	Yoshimatsu, Keiichi, Fruehauf, Krista R, Zhu, Quanhong, Weisman, Adam, Fan, Jun, Xue, Min, Beierle, John M, Rose, Paul E, Aral, Jennifer, Epstein, Linda F, Tagari, Philip, Miranda, Les P, Shea, Kenneth J
Format:	Artikel
Sprache:	eng
Online-Zugang:	Volltext
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