Strontium Laminarin polysaccharide modulates osteogenesis-angiogenesis for bone regeneration
Bone regeneration and repair has become one of the major clinical challenges worldwide and it involves multiple processes including inflammation, angiogenesis and osteogenesis. In this study, we synthesized strontium Laminarin polysaccharide (LP-Sr), a novel polysaccharide-metal complex that should...
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| Veröffentlicht in: | International journal of biological macromolecules 2021-06, Vol.181, p.452-461 |
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| creator | Ma, Fenbo Zhang, Yuanjun Hu, Liqiu Peng, Ying Deng, Yuqing He, Wenqi Ge, Yongmei Tang, Bin |
| description | Bone regeneration and repair has become one of the major clinical challenges worldwide and it involves multiple processes including inflammation, angiogenesis and osteogenesis. In this study, we synthesized strontium Laminarin polysaccharide (LP-Sr), a novel polysaccharide-metal complex that should have therapeutic effects on modulating osteogenesis and angiogenesis. The structure and composition of the as-fabricated LP-Sr were analyzed by EDS, XRD, FITR, 1H NMR, HPLC, etc. The results indicate that we successfully synthesized this novel polysaccharide complex. Moreover, we evaluated the biomedical potential of this complex in promoting osteogenesis and angiogenesis by cell proliferation assay, ALP staining, immunofluorescent staining of CD31 and reverse transcription polymerase chain reaction (RT-PCR). The biological experiment results show that LP-Sr can effectively promote proliferation and increase the expression of VEGF and EGFL6 in HUVECs and significantly up-regulate the expression of Col1α1 and OCN in MC3T3-E1. Besides, it is suggested that LP-Sr has positive effects on the suppression of pro-inflammatory factor IL6 in both HUVECs and MC3T3-E1. Moreover, the osteogenic and angiogenic markers, i.e. alkaline phosphatase (ALP) and CD31, exhibited high expression in LP-Sr group. Hence, we believe that LP-Sr should be a promising and novel polysaccharide complex in modulating osteogenesis-angiogenesis for bone regeneration. |
| doi_str_mv | 10.1016/j.ijbiomac.2021.03.136 |
| format | Article |
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In this study, we synthesized strontium Laminarin polysaccharide (LP-Sr), a novel polysaccharide-metal complex that should have therapeutic effects on modulating osteogenesis and angiogenesis. The structure and composition of the as-fabricated LP-Sr were analyzed by EDS, XRD, FITR, 1H NMR, HPLC, etc. The results indicate that we successfully synthesized this novel polysaccharide complex. Moreover, we evaluated the biomedical potential of this complex in promoting osteogenesis and angiogenesis by cell proliferation assay, ALP staining, immunofluorescent staining of CD31 and reverse transcription polymerase chain reaction (RT-PCR). The biological experiment results show that LP-Sr can effectively promote proliferation and increase the expression of VEGF and EGFL6 in HUVECs and significantly up-regulate the expression of Col1α1 and OCN in MC3T3-E1. Besides, it is suggested that LP-Sr has positive effects on the suppression of pro-inflammatory factor IL6 in both HUVECs and MC3T3-E1. Moreover, the osteogenic and angiogenic markers, i.e. alkaline phosphatase (ALP) and CD31, exhibited high expression in LP-Sr group. Hence, we believe that LP-Sr should be a promising and novel polysaccharide complex in modulating osteogenesis-angiogenesis for bone regeneration.</description><identifier>ISSN: 0141-8130</identifier><identifier>EISSN: 1879-0003</identifier><identifier>DOI: 10.1016/j.ijbiomac.2021.03.136</identifier><identifier>PMID: 33775764</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Bone repair ; Laminarin ; Osteogenesis-angiogenesis ; Strontium</subject><ispartof>International journal of biological macromolecules, 2021-06, Vol.181, p.452-461</ispartof><rights>2021</rights><rights>Copyright © 2021. Published by Elsevier B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c368t-732904f6e18fdd5ca28eaef9b1f28c231709a0c82da0cd6ecd30086260e324c73</citedby><cites>FETCH-LOGICAL-c368t-732904f6e18fdd5ca28eaef9b1f28c231709a0c82da0cd6ecd30086260e324c73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0141813021006784$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33775764$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ma, Fenbo</creatorcontrib><creatorcontrib>Zhang, Yuanjun</creatorcontrib><creatorcontrib>Hu, Liqiu</creatorcontrib><creatorcontrib>Peng, Ying</creatorcontrib><creatorcontrib>Deng, Yuqing</creatorcontrib><creatorcontrib>He, Wenqi</creatorcontrib><creatorcontrib>Ge, Yongmei</creatorcontrib><creatorcontrib>Tang, Bin</creatorcontrib><title>Strontium Laminarin polysaccharide modulates osteogenesis-angiogenesis for bone regeneration</title><title>International journal of biological macromolecules</title><addtitle>Int J Biol Macromol</addtitle><description>Bone regeneration and repair has become one of the major clinical challenges worldwide and it involves multiple processes including inflammation, angiogenesis and osteogenesis. In this study, we synthesized strontium Laminarin polysaccharide (LP-Sr), a novel polysaccharide-metal complex that should have therapeutic effects on modulating osteogenesis and angiogenesis. The structure and composition of the as-fabricated LP-Sr were analyzed by EDS, XRD, FITR, 1H NMR, HPLC, etc. The results indicate that we successfully synthesized this novel polysaccharide complex. Moreover, we evaluated the biomedical potential of this complex in promoting osteogenesis and angiogenesis by cell proliferation assay, ALP staining, immunofluorescent staining of CD31 and reverse transcription polymerase chain reaction (RT-PCR). The biological experiment results show that LP-Sr can effectively promote proliferation and increase the expression of VEGF and EGFL6 in HUVECs and significantly up-regulate the expression of Col1α1 and OCN in MC3T3-E1. Besides, it is suggested that LP-Sr has positive effects on the suppression of pro-inflammatory factor IL6 in both HUVECs and MC3T3-E1. Moreover, the osteogenic and angiogenic markers, i.e. alkaline phosphatase (ALP) and CD31, exhibited high expression in LP-Sr group. Hence, we believe that LP-Sr should be a promising and novel polysaccharide complex in modulating osteogenesis-angiogenesis for bone regeneration.</description><subject>Bone repair</subject><subject>Laminarin</subject><subject>Osteogenesis-angiogenesis</subject><subject>Strontium</subject><issn>0141-8130</issn><issn>1879-0003</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNqFkE1LAzEQhoMoWj_-Qtmjl10nSZvN3hTxCwoe1JsQ0mS2puwmNdkV_Pem1Hr1MsMM7zsv8xAypVBRoOJqXbn10oVem4oBoxXwinJxQCZU1k0JAPyQTIDOaCkphxNymtI6b8WcymNywnldz2sxm5D3lyEGP7ixLxa6d15H54tN6L6TNuYjTxaLPtix0wOmIqQBwwo9JpdK7VduPxRtiMUyeCwibldRDy74c3LU6i7hxW8_I2_3d6-3j-Xi-eHp9mZRGi7kUNacNTBrBVLZWjs3mknU2DZL2jJpGKc1NBqMZDZXK9BYDiAFE4CczUzNz8jl7u4mhs8R06B6lwx2nfYYxqTYPD8OQrImS8VOamJIKWKrNtH1On4rCmpLVq3VnqzaklXAVSabjdPfjHHZo_2z7VFmwfVOgPnTL4dRJePQG7QuohmUDe6_jB_WHI_D</recordid><startdate>20210630</startdate><enddate>20210630</enddate><creator>Ma, Fenbo</creator><creator>Zhang, Yuanjun</creator><creator>Hu, Liqiu</creator><creator>Peng, Ying</creator><creator>Deng, Yuqing</creator><creator>He, Wenqi</creator><creator>Ge, Yongmei</creator><creator>Tang, Bin</creator><general>Elsevier B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20210630</creationdate><title>Strontium Laminarin polysaccharide modulates osteogenesis-angiogenesis for bone regeneration</title><author>Ma, Fenbo ; Zhang, Yuanjun ; Hu, Liqiu ; Peng, Ying ; Deng, Yuqing ; He, Wenqi ; Ge, Yongmei ; Tang, Bin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c368t-732904f6e18fdd5ca28eaef9b1f28c231709a0c82da0cd6ecd30086260e324c73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Bone repair</topic><topic>Laminarin</topic><topic>Osteogenesis-angiogenesis</topic><topic>Strontium</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ma, Fenbo</creatorcontrib><creatorcontrib>Zhang, Yuanjun</creatorcontrib><creatorcontrib>Hu, Liqiu</creatorcontrib><creatorcontrib>Peng, Ying</creatorcontrib><creatorcontrib>Deng, Yuqing</creatorcontrib><creatorcontrib>He, Wenqi</creatorcontrib><creatorcontrib>Ge, Yongmei</creatorcontrib><creatorcontrib>Tang, Bin</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of biological macromolecules</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ma, Fenbo</au><au>Zhang, Yuanjun</au><au>Hu, Liqiu</au><au>Peng, Ying</au><au>Deng, Yuqing</au><au>He, Wenqi</au><au>Ge, Yongmei</au><au>Tang, Bin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Strontium Laminarin polysaccharide modulates osteogenesis-angiogenesis for bone regeneration</atitle><jtitle>International journal of biological macromolecules</jtitle><addtitle>Int J Biol Macromol</addtitle><date>2021-06-30</date><risdate>2021</risdate><volume>181</volume><spage>452</spage><epage>461</epage><pages>452-461</pages><issn>0141-8130</issn><eissn>1879-0003</eissn><abstract>Bone regeneration and repair has become one of the major clinical challenges worldwide and it involves multiple processes including inflammation, angiogenesis and osteogenesis. In this study, we synthesized strontium Laminarin polysaccharide (LP-Sr), a novel polysaccharide-metal complex that should have therapeutic effects on modulating osteogenesis and angiogenesis. The structure and composition of the as-fabricated LP-Sr were analyzed by EDS, XRD, FITR, 1H NMR, HPLC, etc. The results indicate that we successfully synthesized this novel polysaccharide complex. Moreover, we evaluated the biomedical potential of this complex in promoting osteogenesis and angiogenesis by cell proliferation assay, ALP staining, immunofluorescent staining of CD31 and reverse transcription polymerase chain reaction (RT-PCR). The biological experiment results show that LP-Sr can effectively promote proliferation and increase the expression of VEGF and EGFL6 in HUVECs and significantly up-regulate the expression of Col1α1 and OCN in MC3T3-E1. Besides, it is suggested that LP-Sr has positive effects on the suppression of pro-inflammatory factor IL6 in both HUVECs and MC3T3-E1. Moreover, the osteogenic and angiogenic markers, i.e. alkaline phosphatase (ALP) and CD31, exhibited high expression in LP-Sr group. Hence, we believe that LP-Sr should be a promising and novel polysaccharide complex in modulating osteogenesis-angiogenesis for bone regeneration.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>33775764</pmid><doi>10.1016/j.ijbiomac.2021.03.136</doi><tpages>10</tpages></addata></record> |
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| subjects | Bone repair Laminarin Osteogenesis-angiogenesis Strontium |
| title | Strontium Laminarin polysaccharide modulates osteogenesis-angiogenesis for bone regeneration |
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